AN ISO 9001 : 2008 CERTIFIED

INSTITUTE FOR REGENERATIVE MEDICINE

INSTITUTE FOR REGENERATIVE MEDICINE
AN ISO 9001 : 2008 CERTIFIED
STEPPING TOWARDS NEW HORIZON

To all my Patients, Friends and Well-wishers

Chaitanya Stem Center was established one year back with dedication to treat and rehabilitate
disabled children and to make them self-dependant. We believe that nobody deserves a
handicapped life, so we are taking genuine efforts to get rid of the unhealthy, unhappy phase of
your life and to return your smile back to you.
Since last one year, at our Stem Cell Center, we have treated more than 80 patients having
chronic intractable diseases like Cerebral palsy, Mental retardation, Spinal Paraplegia, Autism
and other neurological Disorders etc. We have treated 8 cases of Diabetes Mellitus I & II with
good results.
We are using Bone Marrow Stem Cells / Cord Blood Stem Cells to induce regeneration and
replace the damaged cells in various 75 disorders . We studied 80 cases of Stem Cell
Transplantation with remarkable improvement in around 70 % which were otherwise
untreatable.
I will be glad to extend support of Chaitanya Family to you all and wish you a prosperous , healthy
and blossoming life.

Dr. Anant E. Bagul

M.S. (Ortho.)
 INDEX

Topic Page No.

1. What are stem cells ? 2
2. Stem Cell Sources 2
3. Cerebral Palsy and stem cells 3
4. Diabetes Mellitus 3
5. Cerebrovascular accidents and stroke 4
6. Autism & Stem Cells 4
7. Spinal Cord Injury and stem cells 4
8. Stem Cell Therapy – Principles 5
9. Promise of stem cell research 6
10. Stem cell procedure 7
11. Stem cell collection and processing 8
11. Frequently Asked Questions 10
WHAT ARE STEM CELLS ?

The discovery of the stem cell has lead to a revolution in modern medicine. Stem
cells are the "basic cells" of our bodies, which can develop and differentiate in
any type of cell.
These cells migrate to injured areas within the body and get transplanted and
transform themselves into new tissue cells that replace the damaged ones. Stem
cells have the capacity to multiply and to renew themselves almost indefinitely.
This contrasts with nerve cells, muscle cells and blood cells, which cannot
multiply themselves and have limited life spans.
Stem cells are the foundation cells for every organ, tissue and every cell of body.
Thus first stem cells originate within the developing embryo.

Sources of Stem cells

Adult stem cells:
Stem cells do not completely disappear after birth, but are stored at some sites as
reserves to replenish the damage. These reservoirs can be brought to use by their
(stem cells) extraction from certain sites like bone marrow, peripheral blood and
some tissues
Embryonic stem cells:
Embryonic stem cells are derived from inner cell mass of blastocyst. They fertilize
and divide into different cells. But for obtaining these stem cells the embryo has to
Potentials of Stem Cells
be destroyed in the laboratory which is morally and legally is unacceptable in
* Ability for self renewal indefinately
India.
*Ability to differentiate to desired tissue i.e Brain, pancreas etc..
Cord blood:
Cord blood is a rich source of stem cell. 150 cc of cord blood is collected under
strict aseptic precaution and stored in cord blood bank. Cord blood can be stored
for 21 years and can be used for almost 120 diseases for the same child in future.
CEREBRAL PALSY
functional independence. No single treatment is best and treatment is
Cerebral palsy typically tailored to the child's needs.
Cerebral palsy refers to a group of non-progressive, non-contagious Many medications are used to treat autism spectrum disorder (ASD)
conditions that cause physical disability and applies to the cerebrum in the symptoms that interfere with integrating a child into home or school when
brain and the disorder of movement. behavioral treatment fails. More than half of children diagnosed with ASD
Cerebral palsy cannot be cured but Almost 70% of the cerebral palsy are prescribed psychoactive drugs or anticonvulsants, with the most
patients treated with stem cells at the Chaitanya stem Cell-Center show common drug classes being antidepressants, stimulants, and
improvement. antipsychotics.
Most cerebral palsy patients are treated by Autologous / Cord Blood /
Combined Stem Cell Transplantation by local / Intrathecal / Intra arterial Most Autism patients are treated by Autologous / Cord Blood / Combined
route -in 3 cycles Stem Cell Transplantation by local / Intrathecal / Intra arterial route -in 3
Patient need about 16 days stay cycles
Treatment Patient need about 16 days stay
Follow up statistics from 50 cerebral
palsy patients completed in March
2010 show that close to 67%
experienced improvements after
stem cell therapy.
The type of improvements reported
include: decreased spasticity; better
coordination; improved motor
function, improved posture stability; better cognition resulting in
communication improvements; gaining the ability to sit, stand or even walk
unassisted.Many patients shows significant improvement in their MRI

Autism
Autism is a brain development disorder characterized by impaired social
interaction and communication, and by restricted and repetitive behavior.
These signs all begin before a child is three years old. Autism involves
many parts of the brain. How it occurs is not well understood.
There is no cure for Autism. The main goals of treatment are to lessen
associated deficits and family distress, and to increase quality of life and
CEREBROVASCULAR ACCIDENT SPINAL CORD INJURY
A cerebrovascular accident is also known as a stroke in stroke. An injured spinal cord loses its ability to regenerate myelin-forming cells,
Brain is deprived of adequate oxygen. Strokes are dangerous (with the leading to paralysis. Fehlings showed that where stem cells restored
third leading cause of death). myelin in the injured spine, patients showed some recovery and walked
There are two kinds of strokes: with more coordination.
Ischemic strokes occur when the blood supply to the brain is interrupted, Every year, India gets over 20,000 cases of spinal cord injury patients. A
usually by a blood clot / atherosclerotic plaques originating from the new Mesenchymal stem cell (MSC) therapy could be the answer for these
carotid arteries. patients. The research, conducted by a team of doctors at Bangalore-
Hemorrhagic strokes occur when there is bleeding into or around the based Stem Cell Research, has shown promising results. In the pilot
brain. The risk of stroke increases with age, high blood pressure, a brain study, MSCs were injected directly into the spinal cord of the patient for
injury and carotid artery dissection. maximum efficacy. It was injected either intra-arterially or through intra-
Common stroke consequences: spinal (site of injury) route. Stem Cell Therapy is used routinely in many
Paralysis and loss of feeling, most often in opposite countries with promising results in this un-treatable disease.
body parts
Loss of vision Stem Cells & Spinal Cord Injury
Speaking problems Stem cell therapy can be defined as a part of a group of new techniques, or
Aphasia (inability to communicate) technologies that rely on replacing diseased or dysfunctional cells with
Reasoning and memory disturbances healthy, functioning ones. These new techniques are being applied
Incontinence experimentally to neurological diseases such as Parkinson's disease ,
Changes in character and emotions spinal cord injuries and 120 intractable diseases.
Is there any treatment?
Stroke caused by a blood clot (ischemic stroke) is treated acutely with
different kinds of medications, where as stroke caused by a bleeding in the
vessels (hemorrhagic stroke) is treated surgically. Besides these kinds of
treatments, stem cell therapy can be used to minimize the chronic
consequences of stroke.
Other neurological diseases
Other neurological diseases such us Huntington's
Disease, movement disorders, ataxia, neurological intoxications,
Alzheimer can be treated with the therapies described in this document.
 Stem cell therapy is useful in certain degenerative diseases like
• Parkinson's
STEM CELL THERAPY - PRINCIPLES • Cerebral palsy
• Mental retardation / Autism
• Alzheimer's
Cell-based therapy is an empirical therapy. Stem cell therapy is the use
• Multiple sclerosis
of adult stem cells to treat more than 75 intractable diseases. • Transverse myelitis
Stem cell therapy is the use of stem cells in the therapy. Treatment can be • Hemiplegia / Stroke
divided in three types. There are high hopes from stem cell therapy. Hence there is much on
going research into various types of stem cells, their potential use and
Autologous stem cell therapy- which includes use of patients his own stem their therapeutic applications.
cells obtained from blood, bone marrow etc.

Embryonic stem cell therapy- Embryonic stem cells are derived from inner
cell mass of blastocyst. They fertilize and divide into different cells. But for
obtaining these stem cells the embryo has to be destroyed in the
laboratory which is morally and legally is unacceptable in India.

Allogenic stem cell therapy-includes transplanted stem cells donated by

some other donor. In a number of diseases or disorders, allogenic (i.e.
foreign) stem cells may be rejected by the body.

However Allogenic use of stem cells is not legally acceptable in India,

hence autologous stem cells (adult stem cells) are used in this therapy to
treat certain diseases. The stem cells are the patients own blood bone
marrow and /fat. Stem cells are progenitor cells that lead to creation of new
cells. They are generative cells.
 Identity drug targets and test potential therapeutics
Toxicity Testing
Cultured Pluripotent Stem Cells
Tissues / Cells for Transplantation
Bone marrow for leukemia & chemotherapy
Nerve cells for Parkinsons & Alzhiemer's disease
Heart muscle cells for heart disease
Pancreatic islet cells for diabetes
Stem cell therapy functioning
Study cell differentiation
Understanding prevention & treatment of birth defects
?
The Promise of Stem Cell Research

Stem cell therapy functioning

Stem Cell Therapy - Procedure
The entire procedure consists of the following phases
A. Thorough physical and blood examination will be done by panel of consultants. You will be asked to get PET scan / MRI to check eligibility for the
therapy.
B. Stem cell collection will be done next day. The Chaitanya stem Cell- procedure employs autologous adult stem cells. These cells are collected
from your own bone marrow, Sample will be send to laboratory where the stem cells will be isolated from bone and processing, isolation and
quality checking of the cell will be done.
C. First dose of Isolated stem cell will be given by intrathecal / intralesional injection. You will be discharged on next day. You will need regular
follow-up as recommended by us.
D. Second dose of Stem Cell Injection after 3 month or as per our instruction, & 3rd dose, if needed later on. You will be subjected to repeated PET
scan to monitor progress of stem cell implantation. However the treatment pattern may change as per patients status and disease

Fig 1 :- Local anaesthetic Injection Fig 2:- Palpation of Iliac crest Fig 3:- Inserting needle in Iliac crest Fig 4:- Aspiration of Bone Marrow
/ General Anaesthesia
STEM CELL COLLECTION AND PROCESSING Patient may need 2-3 follow up injections as per the improvement.

Possible Adverse Events from the treatment

Prior to scheduling bone marrow collection, you will be asked to fill out the
After the procedure, you might experience the adverse events. These
patient consent form. This form grants explicit written permission for bone
events can be intense but usually subside within two or three days.
marrow collection.
Nausea
You must discontinue using any blood diluting medications at least 10
Headache
days before your collection date by consulting your physician.
Backache and/or Leg pain
On collection day, we'll have everything prepared to begin collection and
Treatment advantages:
make it comfortable for you. Physician will collect approximately 100 ml of
Simple
bone marrow from your hip bone. Stem cell collection is performed as an
Less invasive than surgery
in-patient procedure under local / general anaesthesia in sterile
Treatment disadvantages:
conditions. It is comparable in pain to a dentist visit. Afterwards, the
Less precise application
collection site might look like a "blue spot" and be a bit painful for 3 - 4 days.
Stem cells are introduced in cerebrospinal fluid via lumbar puncture. Since
Follow-up
the cerebrospinal fluid circulates, stem cells are transported directly to the
Once you have returned home, a member of our medical team will
damaged tissue in the brain or the spinal cord.
regularly monitor your progress via telephone and email. For your
convenience, a telephone 'hotline' is always at your disposal
As with any minimally invasive intervention, intrathecal stem cell
implantation carries a very small risk of infection. We minimize this risk by
working in a sterile environment and by prescribing prophylactic antibiotics
to you. If there are no complications, you can return home after 2 days

For the treatment patient needs 2- 3 days

admission during which his examinations and
various investigations are conducted as per
the International Council – Stem Cell
Research Therapy protocol(IC-SCRT).
During processing the stem cells are
separated according International Quality
STEM CELL PROCESSING
Control protocol. These stem cells are
processed and cryopreserved for further more 2-3 follow up injection
doses. Improvement is evident from 8 weeks onwards to 1 year as stated
according to the previous experiences and studies.

This price includes: Q. 1. What are stem cells?
1. Complete examination by panel of consultants,
2. Thorough blood & other investigations,
3. Bone marrow collection and transportation by international
standard.
4. Stem cell processing, storage and transportation for all three doses for
6 months
Q. 2
5. Intrathecal/ intralesional injection
6. 2 days hospital stay
7. Follow up injection upto 3 doses of stem cell insertion
This price does not include travel expenses such as airfares, meals and
hotel accommodations.
Q. 3 What is stem cell therapy?
General
No additional charges will be incurred unless you are required to extend
your stay at the medical center as a result of complications. Costs do not
include additional surgical treatments. If another treatment is necessary,
We will discuss potential options with you.
Q. 4
You will receive an invoice one week prior to treatment.
This invoice must be paid in-full in Demand draft before treatment can
begin.
Note:
If your bone marrow sample is negative or the stem cells cannot be
administered due to unforeseen medical circumstances, you will only be
required to pay charges incurred to that point. In the case of a negative
bone marrow sample, it might be possible to schedule another bone
marrow collection.
You may be having many questions on stem cells and the therapy. You will
find answers to most of your questions in this brochure. However even
after reading the brochure if you have any queries or you feel like
discussing this topic in detail with our experts; please do not hesitate to
contact us. We have provided with our contact details on the back page of Q. 5
this brochure.
FREQUENTLY ASKED QUESTIONS
Stem cells are the foundation cells for every organ, tissue and
every cell of body. They have capability to differentiate along
cell line or into any type of cell in body. They divide and
multiply within the body. The first stem cells originate
within the developing embryo.
What are the sources of stem cells?
Adult stem cells: involves use of stem cell derived from bone
marrow, peripheral blood and some tissues. Embryonic stem
cells: are derived from inner cell mass of blastocyst. They
fertilize and divide into different cells.
Stem cell therapy is the use of adult stem cells to treat certain
diseases. The stem cells are the patients own blood bone
marrow and /fat. Stem cells are progenitor cells that lead to
creation of new cells. They are generative cells.
What conditions are being treated by stem cell
therapy?
• Stem cell therapy is useful in certain degenerative
diseases like
• Cerebral palsy
• Stroke
• Parkinson's
• Mental retardation
• Alzheimer's
• Multiple sclerosis
• Transverse myelitis
• Hemiplegia
• Autism
What is the procedure for stem cell therapy?
During the stem cell therapy, the stem cells are acquired by
extraction of the bone marrow. The extracted material is sent to
 stem cell based biological laboratory During processing the stem
cells are separated according to the International Quality Control
protocol. These stem cells are introduced in the body to reach
the damaged part and work accordingly.
Q. 6 What examinations and investigations are to be performed
before the therapy?
A few primary blood investigations along with some Radiological
investigations according the diseases are conducted. These help
to understand the chronicity of the disease and provide detailed
information.
Q. 7 How long do the patient need to stay at the clinic?
During the therapy the patient needs to stay at the hospital for
about 2-3 days. However the period of stay depends on the
condition of the patient and severity of the disease.
Q. 8 What amount of results/ progress can be expected from stem
cell therapy?
This is an experimental therapy with expected report up to 30 – 50
%. How ever rare cases may not show desired results. Efficacy of
the treatment varies from patient to patient depending on the
nature and the type of disease.
Q. 9 How many treatments are needed/ given?
Generally 3-4 treatments are given. If more are needed the doctors
st
will address you about it during the 1 appointment. Treatment is
given over a period of 3-5 days depending the disease condition
and recommendation of the doctor.
Q. 10 Can the other treatments be taken at the same time?
Typically will review what other medication the patient is already
on. Medical decision about what should be included or deleted
from the patients regimen during the treatment will therefore be
made on an individual basis.
Q. 11 Are there any known side effects of the therapy?
Doctors who have treated patients have not reported any side
effects other than temporary pain at the site.
Q. 12 Is any special diet required?
No.
Q. 13 Are any nutritional supplements/ medications taken along
with stem cell treatment?
Your treating physician/doctor may prescribe nutritional
supplements that may enhance the benefits of stem cell therapy.
Such prescriptions will be made on an individual basis, depending
on the patients particular evaluation.
Q. 14 Does drinking or smoking affect the therapy?
Alcohol consumption and cigarette smoking both be detrimental to
the new progenitor stem cells. Hence it is advisable that patients do
not smoke or drink during the treatment.
Q. 15 Can I get detailed information on Internet?
You can get the information on Chaitanya hospital Website:
www.edisability.org www.stemcure.co.in
www.chaitanyastemcell.com Similarly on internet the info is
available on www.google.com - search for -“stem cell therapy”
http.//en.wikipeida.org/wiki/stemcell
Q. 16 What is the estimated cost for the treatment?
This therapy is provided for about 20,000 dollars in foreign
countries. However CHAITANYA HOSPITAL provides this therapy
for a very reasonable cost. We follow all the international Quality
Control protocols and rules during the therapy
For more information contact:
CHAITANYA HOSPITAL
RAHI SAKHA APPARTMENT, 133,
Parvati, Pune - Sinhgad Road, Pune - 411 009.
Tel. : 00-91-20-24329666, 020 24328600.
E-mail : anantbagul@yahoo.com