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The purpose of this lab was to calculate the equilibrium constant for the reaction
of iron (III) ions with thiocyanate ions. The reaction is tested under different conditions
to determine if the equilibrium constant always has the same numerical value. In order to
test for this, two parts were necessary for this experiment.
The first part consisted of mixing two sets of solutions. The first of which was a
series of five reference solutions which contained a large excess of Fe3+ ions with known
amounts of SCN- ions. Ideally, the Fe3+ ions should have converted all of the SCN- ions
into the blood red FeSCN2+ ions. Therefore the concentration of the FeSCN2+ in these
solutions should have been equal to the initial concentration of the SCN- ions. The second
set of solutions was a series of five test solutions which contained a constant amount of
Fe3+ ions with varying amounts of SCN- ions. These solutions did not have Fe3+ ions in a
equilibrium.
The second part of this experiment consisted of using colorimetry to find the
spectrophotometer was first calibrated using a test tube filled with distilled water as the
zero absorbance reference. It was then used to find the percent transmittance of each of
the reference and test solutions. The percent transmittance was converted to absorbance
and recorded. The absorbance was then graphed against the concentration of FeSCN2+
ions at equilibrium for the reference solutions. This produced a calibration curve which
was used as a reference which, when combined with the known absorbance values, was
used to calculate the concentration of FeSCN2+ ions at equilibrium in the test solutions.
Once these concentrations were found, the concentrations of Fe3+ ions and SCN- ions at
equilibrium were found for the test solutions and all three sets of values were used to
determine the value of the equilibrium constant for each solution and the then the average
The average value of the equilibrium constant for this reaction was found to be 86
and the average deviation was approximately 8. Despite the great variation in initial
concentrations of the reactants in the test solutions, the values of the equilibrium constant
for each solution were fairly precise. This precision despite the varying initial
There were several sources of error in this lab that produced the lack of exact
precision in the lab. Aside from basic human error such as the natural error in estimating
values when reading measurements, one source of error came from how clean the test
tubes were. The test tubes were cleaned and wiped but were cleaned aggressively or
sterilized of particulates so it is very possible that some dust particles and other
particulates in the air remained of the tubes and contributed to slightly lower percent
transmittance and thus a higher absorbance value than would have been obtained
otherwise. Another possible source of error comes from the fact that the same test tube
was not used for each solution. Absorbance is defined as the product of the molar
concentration of the substance absorbing the light, the absorptivity of the sample, and the
width of the test tube through which the light passes. Because substance that absorbs the
light, the FeSCN2+ ions, is the same in each solution, the absorptivity of the sample
remains constant. Ideally, the width of the test tube would remain constant and the
absorbance would vary directly with the molar concentration of the FeSCN2+ ions.
However because the same test tube was not used for each solution, it is possible that the
width of the test tube varied from solution to solution due to varying radii as well as
varying thicknesses of the glass. This variance would alter the value of the absorbance as
a greater width would produce a higher absorbance value and a smaller width would
produce a lower absorbance value than would have been found otherwise.