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Allele Biotech-Introducing Cost Effectiveness to Research
T
he aim of Allele’s Human REX1-OCT3/4-Nanog Methylation primer sets is to provide researches with -tion statuses of the Oct3/4, Rex1 and NANOG promoter regions. In Takahashi’s 2006 iPS publication, it was dis-covered that these promotor regions were highly unmethyl-ated. Instead of ordering individual oligos, these complete for 50 reactions for each gene. Each batch of primers is vigorously tested for oligo integrity.
 Pre-tested and complete set of PCR primers for: 3 promotor genes.
 All sequences are identical to those as published by Takahashi et al. in their 2006 publication in
Cell
that
 All oligos were produced in-house at Allele Biotech with quality control provided at uniform concentrations for easy reaction setup.
1.
 
Takahashi, K. and S. Yamanaka, Induction of pluri-
 PCR protocol (using Allele-in-One Mix):
follows:
 Denature 94°C for 30 sec Anneal 58°C for 30 sec Anneal and extend 72°C for 1min
Human REX1-OCT3/4-Nanog Methylation
,
set of PCR primers for validation of methylation status of iPSC promoters.
Box 1 | Product InfoHuman REX1-OCT3/4-Nanog Methylation Detection Primer Set
CAT# ABP-SC-iPShMSP 
 
Contents
3 pairs of PCR primers (50μl of 20μM oligo)
FeaturesProtocols
Box 3 | Recommended PCR Setup
ComponentVolumeFinal Conc.
Taq DN
A
 Polymerase
5 unitsNA
Template DN
A
 
(1ng/µl)
 
1µl20 pg/µl
Upstream primer 
 (20 µM)
1 µl 0.6 µM
Downstream gene-
(20 µM)
1 µl 0.6 µM
10 X PCR buffer 
5 µl1X
10 mM dNTP mix
1 µl0.2 mM
Nuclease-free water 
of 50 µl
References
Name
Primers Sequence
mehREX1-F1-S
GGT TTA AAA GGG TAA ATG TGA TTA TAT TTA
mehREX1-F1- AS
CAA ACT ACA ACC ACC CAT CAA C
mehOCT3/4 F2-S
GAG GTT GGA GTA GAA GGA TTG TTT TGG TTT
mehOCT3/4 F2-AS
CCC CCC TAA CCC ATC ACC TCC ACC ACC TAA
mehNANOG-F1-S
TGG TTA GGT TGG TTT TAA ATT TTT G
mehNANOG-F1-AS
 AAC CCA CCC TTA TAA ATT CTC AAT TA

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