Universidade Federal do ABC

BC 1439 ntroduo Bioinformtica

ATIVIDADE PRTICA 1
Turma Diurno 1 quadrimestre 010
Cssia de Soua Carvalho
Prof Ana Carolina Simes
Santo Andr, 14 de maro de 2010
Parte 1.1 - Sequnca A1 e as modadades da ferramenta BAST
> A1
ACCGACATGGCGGCCGTCTTCGCTGTGGTGACTTTAACTCTCGGTTTTCGGTTATAGCCGGCCGGCGCTC
ACTTGTCTTCAGGAAGCTCGGAGCCTTTGGTGGAGCCGGGGAGAGGAAGGGTGGGTGCAAGAGTGAAAGG
CGAGAGGGGACTGCAAGCATCCGGGTCGGCTCCTGGCCGGAGCAAGATGGCTGAGGGCGAGCGGCAGCCG
CCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACTCAGAACTTCATCATTCCAAAAAAGGAGATCCACA
CAGTTCCAGACATGGGCAAATGGAAGCGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTCACCCT
CAACGAAGGTGTGAAGGGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAGGCCATTGAGAAACTAGTC
GCTCTTCTCAACACGCTGGACAGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTTGGGA
ATAAGGCATACAGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCC
TACCCATCTGGCAGCCGCTGTGCCTGAGGTGGCTGTTTACCTAAAGGAGTCAGTGGGGAACTCCACGCGC
ATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGTCTCTGCAAGATTGGGGTGC
TCCGGGTGGATGACCAAATAGCTATTGTCTTCAAGGTGTTCAATCGGTACCTTGAGGTTATGCGGAAACT
CCAGAAAACATACAGGATGGAGCCAGCCGGCAGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTCTG
CCCTTCATCTGGGGCAGTTCGCAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGATGAGA
AGGCCGTGAATGAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGAC
TGGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCCGTCCCTTCCTGGTCCAAAGTGAAC
CAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAGAAGTTCCCTGTGATCCAGCACTTCAAGTTCG
GGAGCCTGCTGCCCATCCATCCTGTCACGTTGGGCTAGGAGGGGCCAAGCCGAAGAGCCACCCAGGCCAC
AGTTCCTGTGCCTGCCTTCCCCACCCCAGCAGTGGCCCCTCCCCATCCCCTCCCTCTGTTCGTCCCGTTT
GATGAGAGGCTGTTTACTGGGGTGGGGTGGCGAGATGGGCTTGAGGGGGCTCAGAGCATAAGGCTTCAGG
GCCCAAGTTGGGAGAAGTGACCAAAGTGTAGCCAGTTTTCTGAGTTCCCGTGTGCTAGACTGGCCAGAAG
AGAGGGTCTGGGGCCTGGTCACTCGGCCACTCTCTCCTGTTTCTGGCCTCTTCTCCCTTCACTCCCGTCC
AGTCTGGTTTTGAGAGCAGGGGCTGTTCTGCAGCACCGCAGGGAAGGGAGGAGAGATACCTGCTGCTTCC
ATTGCTTTTCCCTTCCTGGAGTCGATGCCTTTCTAAGGGTTGGAGCTGCTCCTTGCAGGGGCGGGTCAGT
TTCCCAGGCCATGCCGGG
Utiliou-se a sequncia A1 em formato FASTA primeiramente com a ferramenta
(Figura 1.1.1), que compara uma sequncia dada de nucleotdeos com
sequncias de nucleotdeos dos bancos de dados do NCB. O banco de dados default
(Human genomic plus transcript) foi modificado para Nucleotide collection
(nr/nt), mais abrangente contm All GenBank + Refeq Nucleotides +
MBL + BJ + PB sequences (ecluding HTG0,1,2, T, G, T,
PAT, G Tambm foi alterada para 0000 a opo de nmero mximo de sequncias
alvo a ser mostrado.
Figura 1.1.1 Tela de busca do programa blastn
Os resultados obtidos mostram sequncias com alto score de alinhamento, maior
ou igual a 00 (Figura 1.1.). H sequncias encontradas com cobertura total (coverage,
ou seja, alinhamentos com poucos gaps e mesmo nmero de pares de base), mas h
tambm sequncias com gaps e tamanho diferentes, encontradas em animais
filogeneticamente mais distantes do homem. A identidade varia de 100% a 75%, sendo
que a grande maioria situa-se entre 100% e 99%. O critrio de ordenao default das
tabelas o E value (expectation value, estatisticamente, o valor esperado de acertos a
serem obtidos numa busca ao banco de dados quanto mais baixo o E value, mais
significativo o score). No topo esto as sequncias com e-value 0.0
Figura 1.1. Distribuio grfica dos alinhamentos das sequncias pelo blastn.
A primeira sequncia listada possui score mximo, identidade 100% e E value 0.0.
Trata-se de um cDNA, obtido pelo mNA de Homo sapiens do gene responsvel pela
protena fosfatase famlia A (Figura 1.1.3 e Figura 1.1.4). As trs primeiras sequncias
listadas so de genoma humano, e a quarta a de um primata da famlia Hominidae, Pan
troglodytes, que apresenta uma identidade de 9%, alm de cobertura de 100%. A maioria
das sequncias pertencem a primatas e mamferos.
Figura 1.1.3 Sequncias obtidas, com destaque para a primeira, a de Homo sapiens para
a produo da protena fosfatase A.
Figura 1.1.4 Parte do alinhamento da primeira sequncia, com identidade 100%. O ene
D da sequncia foi destacado em verde.
Usando a mesma sequncia com a ferramenta , que compara uma
sequncia traduida em todos os seis quadros (frames) de leitura com um banco de
dados de protenas. anteve-se o banco de dados Non-redundant protein
sequences (nr)(Figura 1.1.5) e alterou-se o alvo para 0000 sequncias. A matri de
substituio escolhida foi a default BLOUM 62, que apresenta somente resultados com
score de alinhamento igual ou superior a % (Figura 1.1.).
Figura 1.1.5 tela do programa blast.
Figura 1.1. Parmetros do algoritmo, com destaque para a matri de pontuao
utiliada, a BLOUM62.
isualmente, v-se no grfico do blast (Figura 1.1.7) uma diferena em relao
ao blastn realiado anteriormente. mbora os scores de alinhamento continuem altos,
agora a cobertura do alinhamento comparativamente menor m mdia, somente cerca
de 1000 pares de bases esto alinhados sequncia isca, que possui 1 bp. No
aparecem gaps internos s sequncias. sso pode ser explicado pela naturea do
algoritmo de buscas. Como a sequncia de nucleotdeos traduida para cada um dos
seis possveis quadros de leitura, so preditas protenas que existem apenas como
possibilidade, mas no so funcionais, logo podem possuir partes hipotticas que as
verdadeiras protenas identificadas no banco de dados no possuem. ogo, o
alinhamento de uma forma geral apresenta menor cobertura.
Figura 1.1.7 iso grfica do alinhamento das sequncias com o uso do blast
Apenas as oito primeiras sequncias esto com E value 0.0 (Figura 1.1.). A
diferena no E value coincide com alinhamentos de menor cobertura e menor score. A
sequncia com maior score e E value 0.0 foi a do mesmo gene encontrado como primeiro
resultado do blastn.
Figura 1.1. esultados da tela do blast.
Figura 1.1.9 Parte do alinhamento de melhor match, mostrando a protena predita versus
a protena dos bancos de dados pesquisados.
O programa tblast fa a traduo da sequncia de nucleotdeos dada em todos
os seis quadros de leitura possveis e compara com as protenas obtidas pelo mesmo
processo, usando as sequncias de nucleotdeos de seus bancos de dados. sso exige
maior processamento e as buscas tornam-se mais lentas. Na primeira tentativa de
utiliao com A1, tendo como banco de dados Nucleotide collection (nr/nt),
no se completou, sendo apresentada a mensagem de erro no servidor. Pode ser um erro
temporrio, mas repetiu-se at a data do presente relatrio. O banco de dados foi ento
restrito Refeqs, com menor nmero de sequncias, s ento apresentou resultados
(Figura 1.1.10). Pela primeira ve apareceram regies do alinhamento com scores abaixo
de 00, inclusive trechos em que est abaixo dos 40 pontos.
Figura 1.1.10 isualiao dos alinhamentos obtidos com o tblast.
Figura 1.1.11 esultados do tblast. A primeira coluna a de scores e a segunda, a de
E values.
O tblast acaba ento encontrando relacionamentos mais distantes entre as
sequncias. No aparecem tantas sequncias de humanos, e animais mais distantes
evolutivamente esto com E value 0.0, o que no ocorreu na modalidade blast. (Figura
1.1.1) Novamente a primeira sequncia listada, com os melhores resultados de
alinhamento a do gene humano para a fosfatase (Figura 1.1.1).
Figura 1.1.1 Parte do alinhamento da primeira sequncia encontrada no tblast.
Os programas blast e tblast faem a traduo dos nucleotdeos em todos os
seis quadros de leitura. as improvvel obter seis protenas diferentes a partir de cada
quadro. H combinaes em que aparecem muitos cdons de parada, no sendo possvel
determinar um gene. O uso do programa pRA32 dedu os OFs (Open Reading
Frame, uma poro do genoma que pode codificar uma protena) da sequncia (Figura
1.1.13). possvel observar dois possveis OFs em A1, um orientado no sentido 5 3
(nomeado aqui com simples objetivo de diferenciao, de OF1) e outro no sentido 3
5 (OF).
Figura 1.1.13 Tela do softare pDA3 com anlise da sequncia A1. As setas pretas
indicam o tamanho e a orientao dos OFs preditos.
A traduo predita dos OFs em protenas mostrada na Figura 1.1.14. ssas
duas protenas foram posteriormente utiliadas no programa blastp.
Figura 1.1.14 Protenas obtidas a partir dos OFs.
esultado semelhante foi obtido com o uso da ferramenta ORF Finder do NCB.
Foi modificado o parmetro do nmero mdio de aminocidos de 100 para 300. O ORF
Finder tra recursos interessantes. O default mostra protenas iniciadas pelo cdon AT,
mas possvel visualiar uma protena iniciada com CT (boto Alternative Initiation
Codons Figura 1.1.15). Aceitando a sequncia default, visualia-se (boto View) o OF
selecionado e sua protena traduida em formato GenPept (Figura 1.1.1). Tambm
possvel a partir da tela de resultados faer uma busca com o blastp, escolhendo qual
banco de dados e se selecionada a opo com parmetros pode-se ajustar a matri de
pontuao, nmero de sequncias alvo etc.
Figura 1.1.15 esultado obtido com ORF Finder, procurando por OFs com mdia de
300 pares de base e cdon AT como iniciador da sequncia.
LOCU A1 1628 bp linear 10-MAR-2010
FINITION No definition line found.
ACCION A1
VRION
KYOR .
OURC Unknown.
ORGANIM Unknown.
Unclassified.
FATUR Location/Qualifiers
source 1..1628
C 187..1158,73..1158
/note=predicted coding region
/translation=MAGRQPPPAPPATQNFIIPKKIHTVPMGKKRQA
YAYIGFILTLNGVKGKKLTFYRVAIKLVALLNTLRITPPVQPRFGN
KAYRTYAKLANLVATVVPTHLAAAVPVAVYLKVGNTRIYGTGHAAFA
AFLCCLCKIGVLRVQIAIVFKVFNRYLVMRKLQKTYRMPAGQGVGLFQFL
PFIGQLIHPYLPRHFVKAVNNHKYMFLCILFITMKTGPFAHNQL
NIAVPKVNQGLIRMYKACLKFPVIQHFKFGLLPIHPVTLG
BA COUNT 338 a 1 c 87 g 362 t
ORIGIN
1 accgacatgg cggccgtctt cgctgtggtg actttaactc tcggttttcg gttatagccg
61 gccggcgctc acttgtcttc aggaagctcg gagcctttgg tggagccggg gagaggaagg
121 gtgggtgcaa gagtgaaagg cgagagggga ctgcaagcat ccgggtcggc tcctggccgg
181 agcaagatgg ctgagggcga gcggcagccg ccgccagatt cttcagagga ggcccctcca
21 gccactcaga acttcatcat tccaaaaaag gagatccaca cagttccaga catgggcaaa
301 tggaagcgtt ctcaggcata cgctgactac atcggattca tcctcaccct caacgaaggt
361 gtgaagggga agaagctgac cttcgagtac agagtctccg aggccattga gaaactagtc
21 gctcttctca acacgctgga caggtggatt gatgagactc ctccagtgga ccagccctct
81 cggtttggga ataaggcata caggacctgg tatgccaaac ttgatgagga agcagaaaac
51 ttggtggcca cagtggtccc tacccatctg gcagccgctg tgcctgaggt ggctgtttac
601 ctaaaggagt cagtggggaa ctccacgcgc attgactacg gcacagggca tgaggcagcc
661 ttcgctgctt tcctctgctg tctctgcaag attggggtgc tccgggtgga tgaccaaata
721 gctattgtct tcaaggtgtt caatcggtac cttgaggtta tgcggaaact ccagaaaaca
781 tacaggatgg agccagccgg cagccaggga gtgtggggtc tggatgactt ccagtttctg
81 cccttcatct ggggcagttc gcagctgata gaccacccat acctggagcc cagacacttt
01 gtggatgaga aggccgtgaa tgagaaccac aaggactaca tgttcctgga gtgtatcctg
61 tttattaccg agatgaagac tggcccattt gcagagcact ccaaccagct gtggaacatc
1021 agcgccgtcc cttcctggtc caaagtgaac cagggtctca tccgcatgta taaggccgag
1081 tgcctggaga agttccctgt gatccagcac ttcaagttcg ggagcctgct gcccatccat
111 cctgtcacgt tgggctagga ggggccaagc cgaagagcca cccaggccac agttcctgtg
1201 cctgccttcc ccaccccagc agtggcccct ccccatcccc tccctctgtt cgtcccgttt
1261 gatgagaggc tgtttactgg ggtggggtgg cgagatgggc ttgagggggc tcagagcata
1321 aggcttcagg gcccaagttg ggagaagtga ccaaagtgta gccagttttc tgagttcccg
1381 tgtgctagac tggccagaag agagggtctg gggcctggtc actcggccac tctctcctgt
11 ttctggcctc ttctcccttc actcccgtcc agtctggttt tgagagcagg ggctgttctg
1501 cagcaccgca gggaagggag gagagatacc tgctgcttcc attgcttttc ccttcctgga
1561 gtcgatgcct ttctaagggt tggagctgct ccttgcaggg gcgggtcagt ttcccaggcc
1621 atgccggg
//
Figura 1.1.1 Sequncia de bases e sequncia da protena traduida do OF1
As protenas obtidas utiliando-se ambos programas foram as mesmas para seus
respectivos OFs.
Usando as duas sequncias de protenas, foi realiado o ltimo BAST, o ,
que compara uma dada sequncia de protenas com o banco de protenas. Foi
novamente utiliada a matri de substituio BOSU contra 0000 sequncias alvo.
Para identificao, adotou-se Protena1 para aquela predita a partir do OF1 e Protena2
para a correspondente ao OF.
A Figura 1.1.17 mostra parte dos resultados da tela do blastp para a Protena1.
Primeiramente, visualia-se o domnio (elemento estrutural compacto de uma protena
que possui estabilidade prpria)1.
Figura 1.1.17 Domnio da super famlia PTPA encontrado pelo blastp.
A cobertura para os 33 aminocidos quase total, e novamente so encontradas
muitas sequncias com E value 0.0 (Figuras 1.1 e 1.19).
Figura 1.1.1 Distribuio dos alinhamentos no blastp.
Figura 1.1.19 Tela de resultados do blastp.
Figura 1.1.0 Alinhamento completo da sequncia da Protena1 com a protena
Fosfatase A.
Os resultados obtidos no blastp para a Protena traduida a partir do OF so
bem diferentes dos anteriores. -se um alinhamento com baixo score nenhum E value
igual a zero e a baixa identidade (Figuras 1.1 e 1.). A primeira sequncia relacionada
de um organismo distante evolutivamente do homem (Clavispora lusitaniae um fungo
Figura 1.1.3). Biologicamente, no comum de se encontrar em eucariotos dois genes
numa mesma sequncia de mNA capaes de codificar protenas. mbora a Protena
tenha sido predita pelo pRA e pelo ORF Finder, os resultados no encontram
respaldo nas buscas realiadas pelo blastp contra um banco Refeq (he Reference
Sequence collection, um conjunto de sequncias confiveis, testadas e abrangentes,
incluindo DNA genmico, NA e protenas). Conclui-se ento que esta Protena no
deve ser considerada.
Figura 1.1.1 Distribuio dos alinhamentos para a Proteina no blastp.
Figura 1.1. esultados obtidos com blastp para Proteina.
Figura 1.1.3 Alinhamento da protena predita com uma protena hipottica do fungo C
Lusitanae
Por fim, foi realiada a busca da Protena1 com o tblastn, que recebe uma
sequncia de protenas e compara com sequncias traduidas a partir de nucleotdeos
encontrados em seus bancos. Como matri de substituio, usou-se a BLOUM62. O
resultado apresentado nas Figuras 1.1.4, 1.1.5 e 1.1..
So apresentados dados bastante semelhantes aos encontrados com o blastp,
ou seja, alta cobertura e E values 0.0.
Figura 1.1.4 Alinhamentos do tblastn.
Figura 1.1.4 esultados do tblastn, ordenados pelo E value.
Figura 1.1.5 Alinhamento da primeira sequncia encontrada no tblastn. O Gene I
em destaque verde o mesmo encontrado nas buscas anteriores.
Com todas as buscas realiadas, utiliando bancos de dados que incluem Refeq,
conclui-se que o gene da sequncia A1 proveniente de mNA humano e codifica a
protena Fosfatase A. sse gene encontrado no Gene atabase do NCB pelo
identificador 5524uid. Como em todas as buscas esse mesmo Gene I apareceu
sempre em primeiro lugar (exceto para a Protena) com identidade 99% ou 100%, E
value 0.0 e mais alto score, chegou-se sua descrio no banco ene clicando-se no seu
prprio lin (http.ncbi.nlm.nih.govsitesentre
dbgenecmdsearchterm554DT59H7014loggeneexplicitnuclblast_ran
1).
Trs artigos relacionados a esse gene que so de domnio pblico podem ser lidos
online nos endereos a seguir
Peter P. uvolo, ingming Deng, Taahio to, Boyd . Carr and . Stratford ay.
Ceramide Induces Bcl2 ephosphorylation via a echanism Involving itochondrial PP2A
J. Biol. Chem. 1999 74 09-0300. doi10.1074jbc.74.9.09
Full text http.jbc.orgcontent74909.full
Soner Altio, in u and Bruce . Spiegelman PPARy induces cell cycle
withdrawal: inhibition of E2F/P NA-binding activity via down-regulation of PP2A enes
Dev. August 1, 1997 11 197-199; doi10.1101gad.11.15.197
Full text httpgenesdev.cshlp.orgcontent1115197.full
Tournebie, S S Andersen, F erde, Dore, arsenti, and A A Hyman.
istinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics
during mitosis. BO J. 1997 September 15; 1(1) 55375549. doi
10.1093emboj1.1.5537. PCD PC11701
Full text http.ncbi.nlm.nih.govpmcarticlesPC11701pdf005537.pdf
Parte 1.2 - Contedo CG e reges ntrncas e exncas.
A porcentagem de contedo C para a sequncia A1 calculada pelo softare Gene
can, de 5.97% (Figura 1..1).
Figura 1..1 Porcentagem de contedo C pelo Gene can.
O programa pDA oferece um mapa da sequncia, indicando o percentual de
C em cada trecho (Figura 1..). Comparando-se com as regies exnicas, ou seja,
com o OF1, aquele que codifica a protena fosfatase A, v-se pela cores da legenda,
uma presena de C entre 90% e 50%. egies com alto contedo C tendem a
apresentar alta densidade gnica.
Figura 1.. Tela do pDA apresentando a legenda para contedo C.
A ltima anlise para a sequncia A1 foi realiada utiliando-se a ferramenta online
Genome Browser. Os detalhes visualiados na Figura 1..3 pertencem sequncia
encontrada com maior similaridade. O grfico mostra a variao de contedo C ao longo
da sequncia. Novamente apresentado como match o gene para a fosfatase A
humana, e tambm mostrado que esse gene parte do cromossomo 9 humano.
Figura 1..3 Anlise do Genome Browser para a sequncia A1.
Parte 2 - Sequnca GI 289450895
A sequncia de GI 289450895 foi pesquisada no banco Nucleotide do NCB, e
salvou-se a sequncia correspondente no formato FASTA (Anexo 1). Trata-se de um BAC,
(Bacterial Artificial Chromosome), um plasmdeo contendo DNA alheio por instncia, um
fragmento de genoma humano .
Primeiramente foi feita a busca pelo aplicativo oftberr, ferramenta FGNH
(Anexo ). A Figura .1 tra um panorama da quantidade de genes encontrados e de
xons.
Figura .1 Primeira parte da anlise realiada pelo Softberry. egenda mostra nmero de
genes e xons preditos.
Com outra ferramenta utiliada, o aplicativo Gene can, o resultado diferiu no
nmero de genes e xons encontrados. A sada graficamente apresentada na Figura
..
Figura . esultados do ene Scan.
No total, foram preditos 13 genes (Anexo 3).
A busca no aplicativo ORF Finder usando-se essa sequncia apresentou como
resultado (Figura .3) possveis 43 genes, algo bastante improvvel.
Diante de tais diferenas, como determinar o nmero de genes Uma alternativa
realiar buscas no BAST com os genes protenas preditas. uitas das sequncias no
retornam nenhum resultado significativo, ou apresentaro baixa correlao com as
sequncias dos bancos, o que indicar uma predio no respaldada com os dados de
Refeqs, por exemplo. m meio a tantos pares de nucleotdeos, a possibilidade de se
encontrar ao acaso, sequncias que iniciem com AT e terminem com algum stop codon
grande, mesmo que na realidade essa protena nunca tenha sido sintetiada pelo
organismo humano. Como os algoritmos utiliados em cada softare diferem, os
resultados obtidos so distintos.
REFERNCIAS
1 es, Arthur . Introduo Bioinformtica - . ed. - Porto AlegreArtmed, 00.
es, Arthur . Introduction to genomics. Oxford University Press, 007
FGENESH 2. 6 Predict ion of pot ent ial genes in Homo_sapiens genomic DNA
Seq name: t est sequence
Lengt h of sequence: 172772
Number of predict ed genes 9: in + chain 6, in - chain 3.
Number of predict ed exons 53: in + chain 38, in - chain 15.
Posit ions of predict ed genes and exons: Variant 1 from 1, Score: 564. 839160
CDSf CDSi CDSl CDSo PolA TSS
1
16602 18000 19000 20000 21000 22000 23029
1 2
1 + TSS 16602 -5.69
1 + 1 CDSf 16676 - 16680 6.80 16676 - 16678 3
1 + 2 CDSl 22253 - 22400 8.80 22254 - 22400 147
1 + PolA 23029 -1.07
2
24909 40000 50000 60000 70000 80000 90000 103558
1 2 345 6 7891011121314 1516 17 1819 2021 2223 2425 26
2 + TSS 24909 -6.79
2 + 1 CDSf 25535 - 25651 2.10 25535 - 25651 117
2 + 2 CDSi 39151 - 39255 9.80 39151 - 39255 105
2 + 3 CDSi 55474 - 55609 4.56 55474 - 55608 135
2 + 4 CDSi 56061 - 56167 8.97 56063 - 56167 105
2 + 5 CDSi 56475 - 56597 11.32 56475 - 56597 123
2 + 6 CDSi 58519 - 58621 12.50 58519 - 58620 102
2 + 7 CDSi 66323 - 66506 6.54 66325 - 66504 180
2 + 8 CDSi 67209 - 67317 18.88 67210 - 67317 108
2 + 9 CDSi 68082 - 68221 12.73 68082 - 68219 138
2 + 10 CDSi 69249 - 69330 3.06 69250 - 69330 81
2 + 11 CDSi 71040 - 71131 5.68 71040 - 71129 90
2 + 12 CDSi 72005 - 72104 11.39 72006 - 72104 99
2 + 13 CDSi 73072 - 73300 22.19 73072 - 73299 228
2 + 14 CDSi 74834 - 74938 6.89 74836 - 74937 102
2 + 15 CDSi 75394 - 75490 3.26 75396 - 75488 93
2 + 16 CDSi 77055 - 77162 0.17 77056 - 77160 105
2 + 17 CDSi 79581 - 79677 3.68 79582 - 79677 96
2 + 18 CDSi 79924 - 80031 4.39 79924 - 80031 108
2 + 19 CDSi 80967 - 81080 13.03 80967 - 81080 114
2 + 20 CDSi 81426 - 81560 12.87 81426 - 81560 135
2 + 21 CDSi 82740 - 82900 17.62 82740 - 82898 159
2 + 22 CDSi 83493 - 83634 13.80 83494 - 83634 141
2 + 23 CDSi 84601 - 84687 11.45 84601 - 84687 87
2 + 24 CDSi 87824 - 87934 10.73 87824 - 87934 111
2 + 25 CDSi 89109 - 89232 18.70 89109 - 89231 123
2 + 26 CDSl 103238 - 103536 0.58 103240 - 103536 297
2 + PolA 103558 1.12
3
107766 110000 112000 114000 116000 118000 119946
1 2 3 4 5
3 - PolA 107766 1.12
3 - 1 CDSl 107955 - 108051 4.97 107955 - 108050 96
3 - 2 CDSi 108287 - 108417 1.85 108289 - 108417 129
3 - 3 CDSi 111862 - 112032 7.43 111862 - 112032 171
3 - 4 CDSi 112851 - 113006 17.41 112851 - 113006 156
3 - 5 CDSf 117319 - 117489 12.30 117319 - 117489 171
3 - TSS 119946 -8.69
4
120441 121000 121500 122000 122500 122767
1
4 + TSS 120441 -8.59
4 + 1 CDSo 121985 - 122689 21.67 121985 - 122689 705
4 + PolA 122767 -5.58
5
122851 124000 125000 126000 127000 128000 128697
1 2 3 4 5
5 + TSS 122851 -12.79
5 + 1 CDSf 122914 - 123180 17.94 122914 - 123180 267
5 + 2 CDSi 123854 - 124427 12.76 123854 - 124426 573
5 + 3 CDSi 124962 - 125621 15.92 124964 - 125620 657
5 + 4 CDSi 126881 - 126978 7.87 126883 - 126978 96
5 + 5 CDSl 127716 - 128072 -3.82 127716 - 128072 357
5 + PolA 128697 1.12
6
136089 140000 145000 150000 154439
1 2 3 4 5 6 7
6 - PolA 136089 1.12
6 - 1 CDSl 136289 - 136388 1.07 136289 - 136387 99
6 - 2 CDSi 143579 - 143694 -3.28 143581 - 143694 114
6 - 3 CDSi 145710 - 145802 6.49 145710 - 145802 93
6 - 4 CDSi 146768 - 146791 2.75 146768 - 146791 24
6 - 5 CDSi 147026 - 147121 3.46 147026 - 147121 96
6 - 6 CDSi 148162 - 151153 207.93 148162 - 151152 2991
6 - 7 CDSf 154219 - 154298 1.39 154221 - 154298 78
6 - TSS 154439 -5.79
7
155933 156500 157000 157500 158000 158500 159282
1
7 + TSS 155933 -6.59
7 + 1 CDSo 158588 - 158863 20.50 158588 - 158863 276
7 + PolA 159282 1.12
8
159821 160500 161000 161500 162000 162500 162848
1 2 3
8 + TSS 159821 -7.29
8 + 1 CDSf 160522 - 161172 19.33 160522 - 161172 651
8 + 2 CDSi 161236 - 161943 4.69 161236 - 161943 708
8 + 3 CDSl 161999 - 162727 14.76 161999 - 162727 729
8 + PolA 162848 -1.07
9
163073 164000 165000 166000 167000 168000 169000 170000 170878
1 2 3
9 - PolA 163073 1.12
9 - 1 CDSl 164379 - 164397 -5.66 164379 - 164396 18
9 - 2 CDSi 164665 - 164795 14.56 164667 - 164795 129
9 - 3 CDSi 170771 - 170878 11.46 170771 - 170878 108
Predicted protein(s):
>FGENESH:[mRNA] 1 2 exon (s) 16676 - 22400 153 bp, chain +
ATGAAGGAGAAGTTGGAAAAGGATGCAGATCTGGATGGTGTTTTTGCCTGCAGGGAGAAG
TCGGAAAAGGATGCAGATCTGGATGGTGTTTTTGCCTGCAGGGAGAAGTTGGAAAAGGAT
GCAGATCTGGATGGTGTTTCAACAGGGAAATAA
>FGENESH: 1 2 exon (s) 16676 - 22400 50 aa, chain +
MKEKLEKDADLDGVFACREKSEKDADLDGVFACREKLEKDADLDGVSTGK
>FGENESH:[mRNA] 2 26 exon (s) 25535 - 103536 3315 bp, chain +
ATGTTTCTTGGTGGTGGGAGCCCAGGGTTAGTTAGAAGCAGTGAGGGAAGTGTCCCTGAG
AGAATAAACGTCCACGGAATGTTAGCAGAACCTTCTTCTCTGGTAGCTTATGGTCAGGTT
ATTACACCACAACGAAAAATCACTCTGGCTGCACCCAACCGGAAAGACATGGAAGAATGG
ATTAACATCATAAAAACCATCCAACAGGGAGAAATTTATAAGATACCTGCAGCAGAAAAC
AACCCTTTTCTTGTTGGAATGCATTGTTGGTACTCCAGTTACAGCCACCGGACCCAGCAC
TGCAATGTTTGTCGAGAGAGCATTCCTGCCTTATCTAGAGATGCCATCATCTGTGAAGTG
TGCAAAGTGAAATCTCACAGATTGTGTGCTTTGAGAGCAAGCAAAGACTGCAAGTGGAAT
ACATTGTCTATCACTGATGACCTCCTTCTGCCTGCAGATGAAGTAAACATGCCCCATCAA
TGGGTAGAAGGAAACATGCCTGTCAGCTCTCAGTGTGCAGTGTGTCATGAGAGCTGTGGC
AGTTATCAAAGACTTCAAGACTTCCGCTGCCTGTGGTGTAATTCTACGGTGCATGATGAC
TGTAGGAGACGGTTTTCCAAGGAATGTTGCTTCAGAAGCCATCGCTCATCAGTCATTCCT
CCCACTGCTCTAAGCGACCCCAAAGGCGATGGCCAATTAGTAGTATCTTCAGACTTCTGG
AATCTTGATTGGTCATCAGCCTGTTCATGTCCCTTGCTCATCTTCATCAACTCCAAAAGT
GGCGATCATCAGGGGATCGTCTTCCTCCGAAAATTCAAGCAATACCTTAACCCATCTCAA
GTGTTCGACTTATTGAAGGGTGGACCTGAAGCAGGGCTGTCTATGTTCAAGAACTTTGCT
CGCTTTCGCATTCTGGTTTGTGGTGGAGATGGCAGCGTGAGCTGGGTCTTATCTCTGATT
GATGCCTTTGGATTACATGAAAAGTGTCAGTTGGCAGTCATCCCACTTGGAACCGGCAAT
GATCTGGCTCGTGTTCTGGGCTGGGGTGCATTCTGGAACAAAAGCAAGTCACCTCTGGAC
ATCCTCAACAGAGTGGAGCAGGCTAGTGTGAGGATCCTAGACAGATGGAGTGTGATGATT
CGTGAGACTCCCAGACAAACCCCGCTGCTAAAAGGACAGGTTGAAATGGATGTACCACGA
TTTGAGGCTGCTGCCATCCAACACTTAGAATCTGCAGCCACCGAGTTGAACAAAATCCTG
AAGGCCAAGTACCCCACAGAGATGATCATCGCAACCAGATTCTTGTGTTCAGCTGTGGAA
GATTTTGTGGTTGATATTGTAAAGGCCTGGGGTCAGATAAAACAGAACAACACTGCAATA
GTGTCTGTGATTTTGAAAAGTGACTTAATGTATGATAGGCTCAGTGTCCTGATCGATGTC
CTGGCTGAGGAGGCAGCAGCTACTTCTGCTGAAAAAAGTGCTACAGAATATGCAGACAGC
AGCAAGGCAGATAGGAAGCCCTTCATTCCTCAAATAGACCACATAGCCAAGTGCAAGTTG
GAGCTGGCTACAAAGGCCCAGAGTCTCCAGAAATCCTTGAAACTCATCATATTTCAAGTT
GAACAAGCTCTGGATGAGGAAAGCAGACAGACAATATCTGTTAAGAACTTTAGTTCAACT
TTCTTCCTGGAAGATGACCCAGAAGATATTAACCAGACAAGCCCACGACGCCGTTCTCGT
CGTGGCACTTTGTCTTCTATATCTTCTCTCAAAAGTGAGGACCTGGACAACCTTAACTTG
GATCACTTACATTTTACACCTGAATCTATACGCTTCAAAGAAAAATGTGTCATGAACAAC
TACTTCGGAATTGGACTGGATGCTAAAATTTCTCTGGACTTCAACACCAGAAGAGATGAA
CACCCAGGGCAATACAATAGCCGCCTTAAGAACAAGATGTGGTATGGCCTTCTGGGAACC
AAAGAACTTTTGCAGCGCTCTTACAGGAAACTGGAAGAACGAGTGCATTTGGAGTGTGAT
GGAGAAACCATCTCCTTGCCAAACCTGCAAGGCATTGTAGTGCTCAACATTACCAGCTAT
GCTGGAGGTATCAACTTCTGGGGAAGCAACACAGCAACCACGGAATATGAGGCTCCTGCA
ATCGATGATGGGAAACTGGAGGTGGTGGCAATCTTTGGTTCTGTGCAGATGGCAATGTCC
CGTATCATCAACCTGCATCATCATCGCATTGCCCAGTGCCATGAGGTGATGATAACCATT
GATGGTGAAGAAGGTATCCCAGTGCAGGTGGATGGGGAGGCCTGGATTCAGAGACCAGGC
CTTATCAAAATTAGATACAAGAACGCTGCCCAGATGCTGACAAGAGATCGGGACTTTGAG
AACTCAATGAAAATGTGGGAATACAAGCATACTGAAATTCAAGCTGCCCCTCAACCCCAG
CTGGACTTCCAGGACTCTCAAGAGAGCCTCTCTGACGAGGAGTATGCCCAGATGCAGCAC
TTAGCTCGGCTTGCAGAAAACCTCATCAGCAAACTTAATGACCTGAGCAAGATCCACCAG
CATGTGTCTGTCCTCATGGGTTCTGTGAATGCCAGCGCTAACATCCTGAATGATATATTT
TACGGCCAAGACAGTGGCAATGAGATGGGTGCAGCTTCCTGTATTCCCATTGAAACTCTA
AGCAGAAATGATGCCGTAGATGTTACATTTAGTCTTAAAGGTCTCTACGATGACACCACA
GCTTTCCTGGATGAAAAGCTGCTGAGAAGTGCTGAGGATGAGACTGCACTACAAAGCGCC
CTGGATGCCATGAATAAGGAGTTCAAAAAGCTATCTGAGATTGACTGGATGAATCCAATC
TTTGTTCCAGAGGAAAAATCTTCGGACACTGACAGTAGAAGCCTCAGGCTGAAAATTAAG
TTCCCCAAATTGGGAAAGAAAAAGGTAGAAGAGGAACGCAAGCCTAAATCAGGCCAGAGT
GTCCAGAGTTTTATTGGACTAGAAAACACAATCCTAAAATTCATATGGAACCAAAAAAGA
GCCCGCATAGCCAAAGCAAGACTAAGCAAAAAGAACAAATCTGGAGGCATCACATTACTT
GACTTCAAACCATACTATAAGGGTATAGTTACCAAAACAGGATGGTACTGGTATAAAAAT
AGGCACATAGACCAATGGAACAGAATAGAGAACCCAGAAATAAAGCCAAATATGTACAGC
CAACTGATCTTCAACAAAGCAAACAAAAACAAAGTGGAAAAAGAAAACCCTATTCAACAA
ATGGTGTTGGGATAA
>FGENESH: 2 26 exon (s) 25535 - 103536 1104 aa, chain +
MFLGGGSPGLVRSSEGSVPERINVHGMLAEPSSLVAYGQVITPQRKITLAAPNRKDMEEW
INIIKTIQQGEIYKIPAAENNPFLVGMHCWYSSYSHRTQHCNVCRESIPALSRDAIICEV
CKVKSHRLCALRASKDCKWNTLSITDDLLLPADEVNMPHQWVEGNMPVSSQCAVCHESCG
SYQRLQDFRCLWCNSTVHDDCRRRFSKECCFRSHRSSVIPPTALSDPKGDGQLVVSSDFW
NLDWSSACSCPLLIFINSKSGDHQGIVFLRKFKQYLNPSQVFDLLKGGPEAGLSMFKNFA
RFRILVCGGDGSVSWVLSLIDAFGLHEKCQLAVIPLGTGNDLARVLGWGAFWNKSKSPLD
ILNRVEQASVRILDRWSVMIRETPRQTPLLKGQVEMDVPRFEAAAIQHLESAATELNKIL
KAKYPTEMIIATRFLCSAVEDFVVDIVKAWGQIKQNNTAIVSVILKSDLMYDRLSVLIDV
LAEEAAATSAEKSATEYADSSKADRKPFIPQIDHIAKCKLELATKAQSLQKSLKLIIFQV
EQALDEESRQTISVKNFSSTFFLEDDPEDINQTSPRRRSRRGTLSSISSLKSEDLDNLNL
DHLHFTPESIRFKEKCVMNNYFGIGLDAKISLDFNTRRDEHPGQYNSRLKNKMWYGLLGT
KELLQRSYRKLEERVHLECDGETISLPNLQGIVVLNITSYAGGINFWGSNTATTEYEAPA
IDDGKLEVVAIFGSVQMAMSRIINLHHHRIAQCHEVMITIDGEEGIPVQVDGEAWIQRPG
LIKIRYKNAAQMLTRDRDFENSMKMWEYKHTEIQAAPQPQLDFQDSQESLSDEEYAQMQH
LARLAENLISKLNDLSKIHQHVSVLMGSVNASANILNDIFYGQDSGNEMGAASCIPIETL
SRNDAVDVTFSLKGLYDDTTAFLDEKLLRSAEDETALQSALDAMNKEFKKLSEIDWMNPI
FVPEEKSSDTDSRSLRLKIKFPKLGKKKVEEERKPKSGQSVQSFIGLENTILKFIWNQKR
ARIAKARLSKKNKSGGITLLDFKPYYKGIVTKTGWYWYKNRHIDQWNRIENPEIKPNMYS
QLIFNKANKNKVEKENPIQQMVLG
>FGENESH:[mRNA] 3 5 exon (s) 107955 - 117489 726 bp, chain -
ATGCAGATCTGTGTCGTGTGTGTGTTCCTCCAGGTGTCCTTTGAGATGACCCATGAGACC
CTGTACTTGGCAGTGAAGCTGGTGGATCTCTACCTAATGAAGGCAGTATGCAAGAAGGAT
AAGTTACAACTCCTTGGTGCCACTGCCTTTATGATTGCAGCAAAATTTGAGGAGCACAAC
TCACCTCGTGTGGATGACTTTGTGTACATCTGTGATGATAATTATCAGCGATCTGAGGTA
CTCAGCATGGAAATCAACATCCTGAACGTCCTCAAATGTGACATTAACATTCCCATCGCC
TACCATTTTCTGCGCAGATATGCTAGGTGTATCCACACCAACATGAAGACACTGACCTTG
TCCCGCTACATCTGCGAGATGACCCTGCAGGAATACCACTATGTCCAGGAGAAGGCTTCC
AAGCTAGCTGCTGCCTCCTTACTCCTGGCCCTCTACATGAAGAAGCTCGGATACTGGGTA
AACACTTGCGAGATAGGGGTTCCCTTCCTGGAGCATTACAGTGGCTACAGTATCTCTGAG
CTTCACCCCTTGGTCAGACAGCTGAACAAACTGCTGACTTTCAGTTCTTACGATAGTCTC
AAGGCTGTGTATTACAAGTATTCTCACCCGGTCTTCTTTGAAGTCGCCAAAATCCCTGCC
TTGGATATGTTGAAGCTGGAGGAGATTTTGAACTGTGATTGTGAGGCTCAGGGCCTGGTA
CTCTAG
>FGENESH: 3 5 exon (s) 107955 - 117489 241 aa, chain -
MQICVVCVFLQVSFEMTHETLYLAVKLVDLYLMKAVCKKDKLQLLGATAFMIAAKFEEHN
SPRVDDFVYICDDNYQRSEVLSMEINILNVLKCDINIPIAYHFLRRYARCIHTNMKTLTL
SRYICEMTLQEYHYVQEKASKLAAASLLLALYMKKLGYWVNTCEIGVPFLEHYSGYSISE
LHPLVRQLNKLLTFSSYDSLKAVYYKYSHPVFFEVAKIPALDMLKLEEILNCDCEAQGLV
L
>FGENESH:[mRNA] 4 1 exon (s) 121985 - 122689 705 bp, chain +
ATGTTGAAAAATTTCAAAAAGGGATTTAAGGGAGACTATGGAGTTACTATGACACCAGGA
AAACTTAGAACTTTGTGTGAGATAGACTGGCCAGCATTAGAGGTGGGTTGGCCATCAGAA
GGAAGCCTGGACAGGTCCCTTGTCTCGAAGGTATGGCACAGGGGAACCTGTAAGCCAGGG
CACCCAGATCAGTTCCCGTATATAGATTCTTGGTTACAGCTAGTTTTGGATCCCCCACAG
TGGTTAAGAGGACAGGCAGCAGCAGTACTAGTAGCAAAGGGACAGTTAGTTAAGGAAGGC
TGTCACTCCACCCACCGAGGGAAGTCGGCACCAAAAGTCCTGTCCGAGCCAACACCGGAA
GAATCATGGCAGGAATTGGTACCAGCAGTGCCTCCTCCTTATCGAGAGGAAGGGCTCCCC
ATTCCTGAGCCCACAGCACCTCCACTTCCACCAGATATCCATACTCCTAGACCACCCAGA
GTAGACAAAAGAAGAAGTGAAGCCATGGGAGAAACTCCTCCCTTGGCAGCTCACTTACGG
CCCAAGACTGAAATCCAAATGCCCGTGAGAGAACAGCAATATACTGGGGTAGATGAGGAC
AGACACATGGTGAAAAGGCAGCCTTTGTGTATCAACCTTTCACCTCTGCTGACCTCAATT
GGAAAAATAATACTCCAACTTACACCGAAAAGCCTCAAGCTTTAA
>FGENESH: 4 1 exon (s) 121985 - 122689 234 aa, chain +
MLKNFKKGFKGDYGVTMTPGKLRTLCEIDWPALEVGWPSEGSLDRSLVSKVWHRGTCKPG
HPDQFPYIDSWLQLVLDPPQWLRGQAAAVLVAKGQLVKEGCHSTHRGKSAPKVLSEPTPE
ESWQELVPAVPPPYREEGLPIPEPTAPPLPPDIHTPRPPRVDKRRSEAMGETPPLAAHLR
PKTEIQMPVREQQYTGVDEDRHMVKRQPLCINLSPLLTSIGKIILQLTPKSLKL
>FGENESH:[mRNA] 5 5 exon (s) 122914 - 128072 1956 bp, chain +
ATGGAGAGGCTAAGACAGTACCATGCGGCATGGATAGAAGGTCTAAAGAAAGAGGCTCAA
AAGGATACAAATGTAAATAAGGTCTCTGAGGTCATCCAAGGAAAAGAGGAGAGTCCAGCG
CAATTCTATGAACGACTGTGTGAGGCTTACCGTATGTACACTCCTTTTGATCCAGATAGT
CCTGAAAATCAGAGAATGATTAATATGGCCTTAGTCAAAGTGTGGAAGATATCAGGAGAA
AATTGCAGAAACAGGCTGGGTTTGCAGGGCTCTTTACAGCTAAACTTACCAGAAACAGGA
GTTATCATGGCTCTTATGGTCACCAGGGAAAAAGAATGGAGACTTTTTCTGACCGAGCCA
GGCCAAGAGATAAAACCAGCTCTAGCTAAGCGATGGCCCCGAATATGGGCGGATGATAAT
CCTCCGGGACTGGTGGTCAACCAAGACCCTGTACTCATAGAAGTTAAGCCTGGGGCCCAG
CCAATTAGACAAAAGCAGTATCCGGTTCCCAGAGAAGCTCTCGAAGGAATCCAGGTTCAT
CTCAGGCACTTGAAAGCCTTTGGAATTATAGTTCCTTGCCAGTCTCCATGGAACCTCCCC
TTCCTCCCTGTCCCTAAGCCAGGGACCAAGGACTACCAGCCAGTACAGGACTTGTGCTTG
GTCAACCAAGCTACAGTGACTCTGCACCCAACAGTTCCTAACCTTTACACATTGTTAGGG
CTGCTGCAGGCTGAGGACAGCTGGTTTACCTGTCTGGACTTAAAAGATGCCTTCTTTAGC
ATCAGACTAGCTCCTGAAAGCCAGAAGCTGTTTGCCTTTCAGTGGGAAGATCCGGAGTCA
GTCCCAGGACTACCAGATTTGACAAAGCCCTTTACACTCTATGTGTCAGAAAGAGAAAAA
ATGGCAGTTGGAGTTTTAACCCAGACTGTGGGGCCCTGGCCAAGGCCAGTGGTCTATCTC
TCAAAACAACTAGATGGGGTTTCCAAAGTCTGGCCACCATGTCTAAGGGCCCTGGCAGCA
ACAGCCCTGTTAGCACAAGAAGCAGATAAACTAACCCTTGGGCAAAACCTGAATATAAAG
GCCCCCCATGCTGTGGTAACTTTGATGAAAACCAAAGGACATCATTGGCTAACAAATGCT
AGATTAACAAAGTACCAAAGCTTGCTGTGTGAAAATCCCCACATAACCACTGAAGTCTGT
AACACCCTAAATCCCGCCACCCTGCTCCTAGTATCAGAGAGCCTGGTAGAGCATAACTGT
GTAGAGGTGTTGGACTCAGTTTATTCTAGCAGACCTGACCTTTGGGACCAGCCATGGGCA
TCAGTAGACTGGGAGTTATACATGGACGGGAGCAGCTTCATCAACCCACAAGGAGAAAGA
TGTGCAGGATATGTGATGGTAACTTTGGATGCTGTCATTGAAGCCAAACCATTGCCACAG
GGCACTTCAGCCCAGAAGGCTGAGCTCATTGCTTTAACTCGGGCTCTAGAACTCAGTGAA
GGTGACCACGTGTGGATGAAGGATTGGAATGTAGCCCCTTTGCGGCCACAGTGGAAAGGA
CCTCAGACCGTCATCCTGACCACCCCCAAGGCTGTAAAGAAAGGAAAAAGTGGCTCTTCC
TGTACTAAGGGACAATGTAACCCCTTAGAGCTGGTAATAACCAATCCCCTTGATTCTCGC
TGGAAAAAAGGGGAGCGTGTGGCCTTAGGAATCAGTGGGGCCAGACTGAATCCTCGAGTA
AATATCTTAGTTCGAGGAGAAGTTTACAAACGCTCTCCTGAGCCAATGTTTCAAACTTTC
TATGATGAACTACATGTGCCAGTACCAGAAATTCCAGGAAAAACAAGAAATTTGTTTTTG
CAATTAGCCGAGCATGTAGCCCAGTCTCTCAATGTCACTTCATGTTATGTATGTGGAGGA
ATTGTAATGGGAGACCAATGGCCATGGCAAGCCTGA
>FGENESH: 5 5 exon (s) 122914 - 128072 651 aa, chain +
MERLRQYHAAWIEGLKKEAQKDTNVNKVSEVIQGKEESPAQFYERLCEAYRMYTPFDPDS
PENQRMINMALVKVWKISGENCRNRLGLQGSLQLNLPETGVIMALMVTREKEWRLFLTEP
GQEIKPALAKRWPRIWADDNPPGLVVNQDPVLIEVKPGAQPIRQKQYPVPREALEGIQVH
LRHLKAFGIIVPCQSPWNLPFLPVPKPGTKDYQPVQDLCLVNQATVTLHPTVPNLYTLLG
LLQAEDSWFTCLDLKDAFFSIRLAPESQKLFAFQWEDPESVPGLPDLTKPFTLYVSEREK
MAVGVLTQTVGPWPRPVVYLSKQLDGVSKVWPPCLRALAATALLAQEADKLTLGQNLNIK
APHAVVTLMKTKGHHWLTNARLTKYQSLLCENPHITTEVCNTLNPATLLLVSESLVEHNC
VEVLDSVYSSRPDLWDQPWASVDWELYMDGSSFINPQGERCAGYVMVTLDAVIEAKPLPQ
GTSAQKAELIALTRALELSEGDHVWMKDWNVAPLRPQWKGPQTVILTTPKAVKKGKSGSS
CTKGQCNPLELVITNPLDSRWKKGERVALGISGARLNPRVNILVRGEVYKRSPEPMFQTF
YDELHVPVPEIPGKTRNLFLQLAEHVAQSLNVTSCYVCGGIVMGDQWPWQA
>FGENESH:[mRNA] 6 7 exon (s) 136289 - 154298 3501 bp, chain -
ATGGATGAAGCTGGAAACCACTGTTCTCAGCAAACTATCGCAAGGACAAGAAACCAAACA
CCGCATGTTCTCACTCATAGGCATAAGCTGGAAGTCACACCAGTAGTAGCCTCTACTACC
GTGGTACCAAACATTATGGAGAAACCACTCATTCTAGACATATCCACCACCTCCAAAACA
CCCAACACTGAGGAGGCATCTCTCTTCAGAAAGCCATTAGTTTTAAAGGAGGAACCCACT
ATTGAGGATGAAACCCTTATCAATAAGTCATTATCTTTAAAAAAGTGCTCAAATCATGAG
GAGGTGTCCTTACTGGAAAAGCTACAGCCCCTGCAGGAGGAGAGTGACAGTGATGATGCG
TTTGTTATAGAGCCAATGACTTTTAAGAAGACACATAAAACTGAGGAGGCAGCCATCACC
AAGAAGACATTATCCTTAAAGAAGAAGATGTGTGCAAGTCAGCGGAAGCAGTCCTGCCAG
GAAGAGTCGTTGGCTGTGCAGGATGTCAATATGGAAGAGGATTCCTTCTTTATGGAGTCA
ATGAGTTTTAAGAAGAAGCCTAAAACTGAGGAGTCAATCCCCACCCATAAGTTATCATCT
TTAAAGAAGAAATGTACCATTTATGGGAAGATATGCCACTTTAGGAAGCCACCAGTATTG
CAGACAACCATCTGTGGAGCAATGTCCTCCATTAAGAAGCCTACCACTGAGAAGGAGACA
CTTTTCCAAGAGCTATCTGTATTGCAAGAGAAACACACCACTGAGCATGAGATGTCCATC
TTGAAGAAATCATTGGCCTTGCAGAAGACCAACTTTAAAGAGGATTCCCTTGTTAAGGAG
TCGTTAGCCTTTAAGAAGAAGCCTAGCACTGAGGAGGCAATCATGATGCCAGTAATATTG
AAGGAGCAGTGCATGACTGAGGGGAAGAGGTCCCGTCTGAAGCCATTAGTATTGCAGGAG
ATCACCTCTGGAGAGAAGTCGCTCATTATGAAGCCATTGTCCATTAAAGAAAAGCCATCT
ACTGAGAAGGAGTCCTTTTCCCAGGAACCATCTGCATTGCAAAAGAAGCACACCACTCAG
GAGGAGGTTTCCATCTTAAAGGAGCCCTCGTCCTTGCTAAAGTCTCCAACTGAGGAGTCA
CCTTTTGATGAGGCTTTGGCTTTTACAAAGAAGTGTACCATTGAGGAGGCACCCCCCACC
AAGAAGCCTTTAATTTTAAAGAGGAAGCATGCCACTCAGGGGACAATGTCCCACTTGAAG
AAACCACTAATATTACAGACCACCTCTGGAGAAAAGTCACTTATTAAGGAGCCACTGCCC
TTTAAAGAAGAAAAAGTGTCTTTAAAGAAAAAGTGTACCACACAAGAGATGATGTCCATC
TGTCCAGAACTGTTGGACTTTCAGGATATGATTGGTGAAGATAAGAATTCTTTCTTTATG
GAGCCAATGTCATTTAGGAAGAACCCTACAACTGAGGAGACAGTACTTACCAAGACATCG
TTGTCTTTACAGGAAAAGAAAATTACTCAGGGGAAGATGTCCCACTTAAAGAAGCCACTG
GTCTTGCAGAAGATCACTTCTGAGGAGGAGTCATTCTATAAGAAGCTGTTGCCCTTTAAG
ATGAAATCTACAACGGAAGAAAAGTTCCTCTCCCAGGAACCATCTGCATTGAAAGAGAAG
CATACCACCTTGCAGGAAGTGTCCCTCTCAAAAGAGTCATTGGCCATCCAAGAGAAGGCT
ACCACTGAGGAGGAATTCTCTCAGGAACTATTTTCATTGCATGTTAAGCATACCAACAAA
AGTGGGTCCCTCTTCCAGGAGGCTTTGGTCTTGCAAGAGAAGACTGATGCCGAAGAGGAT
TCCTTGAAGAACTTGTTGGCTTTGCAGGAGAAAAGCACCATGGAAGAAGAGTCCCTTATC
AATAAGCTATTGGCTCTGAAGGAGGAGCTTTCTGCTGAGGCAGCCACAAACATACAGACA
CAATTATCTTTAAAGAAGAAGTCCACTTCTCATGGAAAAGTGTTCTTCCTGAAGAAGCAG
TTGGCTTTGAATGAGACCATCAATGAAGAGGAGTTCCTTAATAAGCAGCCACTGGCCTTG
GAGGGGTATCCCAGCATTGCGGAGGGGGAGACCCTCTTCAAGAAGCTTTTGGCCATGCAG
GAGGAGCCCAGCATTGAGAAGGAAGCTGTCCTCAAGGAGCCCACTATTGACACAGAAGCT
CACTTTAAGGAACCTTTGGCCTTGCAGGAGGAGCCCAGCACTGAGAAGGAGGCTGTCCTC
AAGGAGCCCAGTGTTGACACAGAAGCTCACTTTAAGGAAACTTTGGCCTTGCAGGAGAAG
CCCAGCATTGAGCAGGAGGCCCTCTTTAAGCGACACTCAGCTTTGTGGGAGAAGCCCAGC
ACTGAGAAGGAGACCATCTTCAAGGAGTCTTTGGACTTGCAAGAGAAGCCCAGCATTAAG
AAAGAGACCCTCCTCAAAAAGCCATTAGCCTTGAAGATGTCTACCATCAATGAGGCAGTC
CTCTTCGAAGATATGATAGCTCTGAATGAGAAACCCACCACTGGGAAGGAGTTGTCCTTC
AAGGAGCCATTAGCCTTACAAGAGAGTCCCACCTACAAGGAAGACACCTTTCTCAAAACA
TTGTTGGTCCCCCAAGTTGGAACCAGCCCAAATGTGTCTAGCACTGCCCCTGAATCCATA
ACCAGCAAGTCCAGCATTGCTACCATGACCAGTGTGGGCAAATCTGGTACCATCAATGAG
GCATTCCTCTTCGAAGATATGATAACTCTGAATGAGAAACCCACCACTGGGAAGGAGTTG
TCCTTCAAGGAGCCATTGGCCTTACAAGAGAGTCCCACCTGCAAGGAAGACACCTTTCTG
GAAACATTCTTGATCCCCCAAATTGGAACCAGCCCATATGTGTTTAGCACCACCCCTGAA
TCCATAACAGAGAAGTCCAGCATTGCAACCATGACCAGCGTGGGCAAGTCCAGGACCACC
ACCGAGTCCAGTGCATGTGAATCTGCTTCTGATAAACCTGTCTCACCACAGGCCAAGGGA
ACACCAAAGGAGATAACCCCACGGGAAGATATTGATGAGGACAGCAGTGATCCAAGTTTC
AACCCAATGTATGCCAAGGAAATCTTCAGTTACATGAAAGAGAGAGAGATAGAGGAAACT
GCCCAGAGTCATGAACAGTTTATACTTACAGATTACATGAACAGGCAGATTGAAATCACC
AGTGACATGAGGGCCATTCTTGTGGACTGGTTGGTGGAGGTGCAGTATTCCATGGTACAG
ATGTACCACGGTAATATGGTTTGGCTATGTCCACACCCAAATCTTATCTTGAATTATAGC
TCCCATAATCCCCACGTGTTGTGGGAGGGACGCAGTGGGAGGGACATGGATGAAGCTGGA
AACCATCGTTCTCAGCAAACTATCGCAAGGACAAAAAACCAAACACCGCATGTTCTCACT
CAGGTGGGAGTTGAACAATGA
>FGENESH: 6 7 exon (s) 136289 - 154298 1166 aa, chain -
MDEAGNHCSQQTIARTRNQTPHVLTHRHKLEVTPVVASTTVVPNIMEKPLILDISTTSKT
PNTEEASLFRKPLVLKEEPTIEDETLINKSLSLKKCSNHEEVSLLEKLQPLQEESDSDDA
FVIEPMTFKKTHKTEEAAITKKTLSLKKKMCASQRKQSCQEESLAVQDVNMEEDSFFMES
MSFKKKPKTEESIPTHKLSSLKKKCTIYGKICHFRKPPVLQTTICGAMSSIKKPTTEKET
LFQELSVLQEKHTTEHEMSILKKSLALQKTNFKEDSLVKESLAFKKKPSTEEAIMMPVIL
KEQCMTEGKRSRLKPLVLQEITSGEKSLIMKPLSIKEKPSTEKESFSQEPSALQKKHTTQ
EEVSILKEPSSLLKSPTEESPFDEALAFTKKCTIEEAPPTKKPLILKRKHATQGTMSHLK
KPLILQTTSGEKSLIKEPLPFKEEKVSLKKKCTTQEMMSICPELLDFQDMIGEDKNSFFM
EPMSFRKNPTTEETVLTKTSLSLQEKKITQGKMSHLKKPLVLQKITSEEESFYKKLLPFK
MKSTTEEKFLSQEPSALKEKHTTLQEVSLSKESLAIQEKATTEEEFSQELFSLHVKHTNK
SGSLFQEALVLQEKTDAEEDSLKNLLALQEKSTMEEESLINKLLALKEELSAEAATNIQT
QLSLKKKSTSHGKVFFLKKQLALNETINEEEFLNKQPLALEGYPSIAEGETLFKKLLAMQ
EEPSIEKEAVLKEPTIDTEAHFKEPLALQEEPSTEKEAVLKEPSVDTEAHFKETLALQEK
PSIEQEALFKRHSALWEKPSTEKETIFKESLDLQEKPSIKKETLLKKPLALKMSTINEAV
LFEDMIALNEKPTTGKELSFKEPLALQESPTYKEDTFLKTLLVPQVGTSPNVSSTAPESI
TSKSSIATMTSVGKSGTINEAFLFEDMITLNEKPTTGKELSFKEPLALQESPTCKEDTFL
ETFLIPQIGTSPYVFSTTPESITEKSSIATMTSVGKSRTTTESSACESASDKPVSPQAKG
TPKEITPREDIDEDSSDPSFNPMYAKEIFSYMKEREIEETAQSHEQFILTDYMNRQIEIT
SDMRAILVDWLVEVQYSMVQMYHGNMVWLCPHPNLILNYSSHNPHVLWEGRSGRDMDEAG
NHRSQQTIARTKNQTPHVLTQVGVEQ
>FGENESH:[mRNA] 7 1 exon (s) 158588 - 158863 276 bp, chain +
ATGGCAAAGAAGTTAAAAACTTTGAAAAAAAAAATAGACGAATGGCTAACTAGAATAACC
AATGCAGAGAAGTCCTTAAAGGACCTGATGGAGCTGAAAACCATGGCACGAGAACTACGT
GACGAATGCACAAGCCTCAGTAAACAAGGCGATCAACTGGAAGAAAGGGTATCAGCAACG
GAAGACGAAATGAATGAAATGAAGCATGAAGAGAAGTTTAGAGAAAAAGAATACAAAGAA
ATGAACAAAGCCTCCAAGAAATATGGGACTATGTGA
>FGENESH: 7 1 exon (s) 158588 - 158863 91 aa, chain +
MAKKLKTLKKKIDEWLTRITNAEKSLKDLMELKTMARELRDECTSLSKQGDQLEERVSAT
EDEMNEMKHEEKFREKEYKEMNKASKKYGTM
>FGENESH:[mRNA] 8 3 exon (s) 160522 - 162727 2088 bp, chain +
ATGAAGGCAATAGAGACACAAAAATCCCTTCAAAAAATCAATGAATCCAGGAGCTGGTTT
TTTGAAAAGATCAACAAAATTGATAGACCGCTAGCAAGAATAATAAAGAAGAAAACAGAG
AAGAATCAAATAGACGCAATAAAAAATGACAAAGGGGATATCACCACCGATCCCACAGAA
ATACAAACTACCATCAGAGAATACTATAAACACCTCTACACAAATAAACTAGAAATTCTA
GAAGAAATGGATAAATTTTTCGACACATACACTCTCCCAAGACTAAATCAGGAAGAAGTT
GAATCTCTGAATAGACAAATAACAGACTCTGAAATTGAGGCAATAATTAATAGCTTACCA
ACCAAAAAAAGTCCAGGACCAGATGGATTCACAGCCGAATTCTACCAGAGGTACAAGGAG
GAGCTGGTACCATTCCTTCTGAAACTATTCCAATCAATAGAAAAAGAGGGAATCCTCCCT
AACTCATTTTATGAGGCCAGCATCATTCTGATACCAAAGCCTGGCAGAGACACAACAAAA
AAAGAGAATTTTAGACCAATATCTTTGATGAACATCGATGCAAAAATCCTCAATAAAATA
CTGGCAAAAGGAATCCAGCAGCACAGCAAAAAGCTTATCCACCATGATCAACATTTAAAC
AGAACCAAAGACAAAAACCACATGATTATCTCAATAGATGCAGAAAAGGCCTTTGACAAA
ATTCAACAACCCTTCATGCTAAAAACTCTCAATAAATTAGGTATTGATGGGACGTATCTC
AAAATAATAAGAGATATCTGTGACAAACCCACAGCCAATATCATACTGAATGGACAAAAA
CTGGAAGCATTCCCTTTGAAAACTGGCACAAGACAGGGATGCCCTCTCTCACCACTCCTA
TTCAACATAGTGTTGGAAGTTCTGGCCAGGGCAATCAGGCAGCAGAAGGAAATAAAGGGC
ATTCAATTAGGAAAAGAGGAAGTCAAATTGTCCCTGTTTGCAGATGACATGATTGTATAT
CTAGAAAACCCCATTGTCTCAGCCCAAAATCTCCTTAAGCTAATAAGCAACTTCAGCAAA
TTCTCAGGATACAAAATCCATGTGCAAAAATCACAAGTATTCTTATACACCAATAACAGA
CAAACAGAGAGCCAAATCATGAGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAA
TACCTAGGAATCCAACTTACAAGGGATGTGAAGGACCTTTTCAAGAAGAACTACAAACCA
CTGCTCAATGAAATAAAAGAGGATACAAACAAATGGAAGAACATTCCATGCTCATGGGTA
GGAAGAATCAATATTGTGAAAATGGCCATACTGTCCAAGAATTGGAAAAAAACTACTTTA
AAGTTCATATGGAACCAAAAAAGAGCCCGCATTGACAAGTCAATCCTAAGCCAAAAGAAC
AAAGCTGGAGGCATCACGCTACCTGACTTCAAACTATACTACAAGGCTATAGTAACCAAA
ACAGCATGGTACTGGTACCAAAACAGAGATATAGACCAATGGAACAGAACAGAGCCCTCA
GAAATAATACCACACATCAACAACTATCTGATCTTTGACAAACCTGACAAAAACAAGCAA
TGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACTGGCTAGCCATATGTAGA
AAGCTGAAACTGGATCCCTTCCTTTCACCTTATACAAAAATTAATTCAAGATGGATTAAA
GACTTAAATGTTAGACCTAAAACCATAAAAACCCTAGAAGAAAACCTAGGCAATACCATT
CAGGACATAGGCATGGGCAAGGATTTCATGTCTAAAACACCAAAAGCAATGGCAACAGAA
GCCAAAATTGACAAATGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCAAAAGAAACC
ACCATCAGAGTGAACAGGAAACCTACAGAATGGGAGAAAATTTTTGCAACCTACTCATCT
GACAAAGGGCTAATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAAGCA
AACAACCCCATCAAAAAGTGGGTGAAGGATATGAACAGACATTTCTGA
>FGENESH: 8 3 exon (s) 160522 - 162727 695 aa, chain +
MKAIETQKSLQKINESRSWFFEKINKIDRPLARIIKKKTEKNQIDAIKNDKGDITTDPTE
IQTTIREYYKHLYTNKLEILEEMDKFFDTYTLPRLNQEEVESLNRQITDSEIEAIINSLP
TKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTK
KENFRPISLMNIDAKILNKILAKGIQQHSKKLIHHDQHLNRTKDKNHMIISIDAEKAFDK
IQQPFMLKTLNKLGIDGTYLKIIRDICDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLL
FNIVLEVLARAIRQQKEIKGIQLGKEEVKLSLFADDMIVYLENPIVSAQNLLKLISNFSK
FSGYKIHVQKSQVFLYTNNRQTESQIMSELPFTIASKRIKYLGIQLTRDVKDLFKKNYKP
LLNEIKEDTNKWKNIPCSWVGRINIVKMAILSKNWKKTTLKFIWNQKRARIDKSILSQKN
KAGGITLPDFKLYYKAIVTKTAWYWYQNRDIDQWNRTEPSEIIPHINNYLIFDKPDKNKQ
WGKDSLFNKWCWENWLAICRKLKLDPFLSPYTKINSRWIKDLNVRPKTIKTLEENLGNTI
QDIGMGKDFMSKTPKAMATEAKIDKWDLIKLKSFCTAKETTIRVNRKPTEWEKIFATYSS
DKGLISRIYNELKQIYKKKANNPIKKWVKDMNRHF
>FGENESH:[mRNA] 9 3 exon (s) 164379 - 170878 258 bp, chain -
ACGGGGGAGAATTGCCAAACGAAGATATCTCCATCTTCACTTCAGGAGTCTCCATCTTCA
CTTCAGGGAGCACTCAAAAAGAGATCAGCTTTTGAAGATCTCACTAATGCTTCTCAATGT
CAACCTGTCCAGCCCAAGAAAGAAGCCAATAAAGAGTTTGTAAAAGTTGTTTCCAAGAAG
ATAAACAGGAACACACATGCTCTTGGACTGGCCAAAAAGAATAAGCGGAATCTAAAATGT
AATTCAGTACCTGTTTGA
>FGENESH: 9 3 exon (s) 164379 - 170878 85 aa, chain -
TGENCQTKISPSSLQESPSSLQGALKKRSAFEDLTNASQCQPVQPKKEANKEFVKVVSKK
INRNTHALGLAKKNKRNLKCNSVPV
GENSCAN Output
View gene model output: PS | PDF
GENSCAN 1.0 Date run: 14-Mar-110 Time: 19:06:16
Sequence /tmp/03_14_10-19:06:14.fasta : 172778 bp : 40.67% C+G : Isochore 1 ( 0 - 43 C+G%)
Parameter matrix: HumanIso.smat
Predicted genes/exons:
Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------
1.02 Intr - 3385 3245 141 1 0 22 90 87 0.333 1.93
1.01 Init - 5876 5796 81 2 0 65 96 65 0.928 6.02
1.00 Prom - 5984 5945 40 -2.25
2.00 Prom + 7347 7386 40 -6.25
2.01 Init + 8676 8887 212 2 2 91 35 134 0.499 6.70
2.02 Term + 13352 13808 457 2 1 10 44 205 0.125 1.91
2.03 PlyA + 15360 15365 6 1.05
3.00 Prom + 16606 16645 40 -3.05
3.01 Init + 16682 16686 5 1 2 103 100 0 0.741 2.22
3.02 Intr + 22259 22386 128 2 2 43 -8 228 0.622 8.10
3.03 Intr + 25325 25516 192 0 0 127 -30 115 0.169 2.14
3.04 Term + 27429 27652 224 1 2 43 53 156 0.278 3.70
3.05 PlyA + 28461 28466 6 -0.45
4.00 Prom + 28809 28848 40 -6.35
4.01 Init + 28936 29079 144 0 0 83 23 95 0.274 2.67
4.02 Intr + 37138 37218 81 0 0 83 75 39 0.534 1.02
4.03 Intr + 39157 39261 105 0 0 43 116 121 0.948 9.89
4.04 Term + 44436 44534 99 2 0 88 42 95 0.943 2.05
4.05 PlyA + 45122 45127 6 1.05
5.05 PlyA - 45149 45144 6 1.05
5.04 Term - 48535 48471 65 2 2 90 36 39 0.568 -4.03
5.03 Intr - 48916 48743 174 2 0 85 52 66 0.464 1.69
5.02 Intr - 51653 51480 174 0 0 68 81 131 0.817 9.39
5.01 Init - 51985 51868 118 1 1 72 61 103 0.912 6.41
5.00 Prom - 52350 52311 40 -6.95
6.00 Prom + 53595 53634 40 -4.95
6.01 Init + 53726 53905 180 1 0 51 58 55 0.814 -1.97
6.02 Intr + 55480 55615 136 0 1 84 77 98 0.885 7.52
GENSCAN Output http://genes.mit.edu/cgi-bin/genscanw_py.cgi
6.03 Intr + 56067 56173 107 1 2 105 86 43 0.944 4.81
6.04 Intr + 56481 56603 123 2 0 112 107 40 0.995 8.16
6.05 Intr + 58525 58627 103 0 1 147 101 56 0.998 11.63
6.06 Intr + 66329 66512 184 0 1 85 98 112 0.976 9.82
6.07 Intr + 67215 67323 109 0 1 146 115 69 0.999 14.77
6.08 Intr + 68088 68227 140 2 2 90 111 140 0.998 14.84
6.09 Intr + 69255 69336 82 0 1 78 78 72 0.998 3.92
6.10 Intr + 71046 71137 92 2 2 73 82 150 0.854 10.77
6.11 Intr + 72011 72110 100 2 1 97 106 64 0.999 8.29
6.12 Intr + 73078 73306 229 0 1 102 116 202 0.996 20.92
6.13 Intr + 74840 74944 105 0 0 94 69 128 0.999 10.77
6.14 Intr + 75400 75496 97 2 1 81 92 79 0.944 5.75
6.15 Intr + 77061 77168 108 0 0 46 80 95 0.740 2.98
6.16 Intr + 79587 79683 97 0 1 42 88 67 0.579 1.19
6.17 Intr + 79930 80037 108 0 0 69 61 104 0.778 5.36
6.18 Intr + 80973 81086 114 2 0 53 96 214 0.874 18.42
6.19 Intr + 81432 81566 135 2 0 95 90 106 0.992 11.34
6.20 Intr + 82746 82906 161 2 2 70 108 203 0.999 18.36
6.21 Intr + 83499 83640 142 0 1 86 100 166 0.999 17.03
6.22 Intr + 84607 84693 87 0 0 113 105 125 0.999 15.95
6.23 Intr + 87830 87940 111 1 0 82 92 103 0.997 9.76
6.24 Intr + 89115 89238 124 2 1 104 111 80 0.995 11.14
6.25 Intr + 89287 89406 120 2 0 10 100 88 0.481 1.75
6.26 Term + 90645 90724 80 1 2 64 41 79 0.433 -2.25
6.27 PlyA + 90767 90772 6 -0.45
7.10 PlyA - 90991 90986 6 1.05
7.09 Term - 93770 93591 180 2 0 50 43 118 0.751 0.13
7.08 Intr - 94489 94280 210 1 0 75 84 148 0.877 11.29
7.07 Intr - 105109 104838 272 2 2 38 49 157 0.424 3.14
7.06 Intr - 108057 107987 71 2 2 99 40 54 0.575 -0.39
7.05 Intr - 108423 108293 131 0 2 61 105 100 0.716 7.57
7.04 Intr - 112038 111889 150 0 0 70 32 201 0.577 12.14
7.03 Intr - 113012 112857 156 2 0 116 90 197 0.999 21.99
7.02 Intr - 117422 117325 98 0 2 69 105 44 0.542 3.01
7.01 Init - 117744 117537 208 0 1 43 70 130 0.549 5.73
7.00 Prom - 119993 119954 40 -9.35
8.00 Prom + 120443 120482 40 -7.15
8.01 Init + 121991 122599 609 1 0 54 22 503 0.722 35.66
8.02 Intr + 122803 123186 384 0 0 -36 80 320 0.870 12.92
8.03 Intr + 123569 124433 865 1 1 -11 38 492 0.644 24.29
8.04 Intr + 124542 124792 251 1 2 9 80 209 0.398 8.43
8.05 Intr + 124873 124933 61 0 1 30 78 38 0.448 -5.61
8.06 Term + 124968 125875 908 1 2 17 48 471 0.396 27.47
8.07 PlyA + 125998 126003 6 1.05
GENSCAN Output http://genes.mit.edu/cgi-bin/genscanw_py.cgi
9.00 Prom + 126009 126048 40 -18.05
9.01 Init + 126077 126308 232 1 1 70 75 182 0.509 13.57
9.02 Intr + 126887 126984 98 0 2 102 70 101 0.820 8.51
9.03 Term + 127752 128078 327 2 0 -1 54 171 0.288 -1.48
9.04 PlyA + 128703 128708 6 1.05
10.06 PlyA - 131375 131370 6 -0.45
10.05 Term - 132647 132588 60 2 0 86 55 53 0.145 -1.37
10.04 Intr - 145808 145716 93 2 0 62 115 87 0.973 8.04
10.03 Intr - 146797 146774 24 1 0 84 103 23 0.618 0.70
10.02 Intr - 147127 147032 96 1 0 52 53 126 0.796 4.79
10.01 Init - 151104 148168 2937 0 0 59 51 2476 0.843 232.51
10.00 Prom - 154481 154442 40 -3.65
11.00 Prom + 156034 156073 40 -5.05
11.01 Sngl + 158594 158869 276 1 0 47 48 247 0.614 11.73
11.02 PlyA + 159288 159293 6 1.05
12.00 Prom + 159823 159862 40 -6.15
12.01 Sngl + 160528 161220 693 0 0 83 48 267 0.767 18.15
12.02 PlyA + 161226 161231 6 1.05
13.05 PlyA - 163084 163079 6 1.05
13.04 Term - 164403 164385 19 2 1 92 49 12 0.565 -5.49
13.03 Intr - 164801 164671 131 2 2 133 101 144 0.745 18.77
13.02 Intr - 170884 170777 108 1 0 93 82 103 0.993 9.76
13.01 Intr - 171464 171405 60 2 0 41 72 86 0.333 0.31
Suboptimal exons with probability > 1.000
Exnum Type S .Begin ...End .Len Fr Ph B/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------
NO EXONS FOUND AT GIVEN PROBABILITY CUTOFF
Predicted peptide sequence(s):
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_1|74_aa
MGNPEGKRKHCRPSDGTHKESSQPEKRIKLGLPKEHQSLAVASQSSQKRESLGIVLLTPS
SKFKCQVQEKFDTK
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_2|222_aa
MADTISDAWIYIHKTNWREQGYSVYALTLQHLLLLRDLSSDIKFGYPDKVSKILRLLTVD
DIFYSQLSISSTGSPGQSNQARERNKGHPNRKRGSQTILFADNMILYLENPIVLAPKLLQ
GENSCAN Output http://genes.mit.edu/cgi-bin/genscanw_py.cgi
LINNFSKLSRYKINLEKPLAFLYTNNSQAKSQIRNAIPFTIAIKGIKYLRIQLTKEVKNL
YKDNYNTLVKEIRDDTNTWKNIPCSWMGRINIIKMTTLPKEI
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_3|182_aa
MKEKLEKDADLDGVFACREKSEKDADLDGVFACREKLEKDADLDGLWLRALNKIMHVKQG
QIAGICQPETNLFLWRRRVEEKLREEIATPAASNEGHRQSHNRSHSSHRSSGRRFADTVL
ADLRGRKIWLFSVRMTTRSQFAQDPPDCSAETPGKTRTVGHPIPHPAAAALRQPGGKIPV
VA
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_4|142_aa
MDLGIGLSKRIQNETEAEFSKALAFSARAAMVLAKTILCEHCTQDLNYFAHFETIDLSQA
TVAESSCRNLCHSFCVITPQRKITLAAPNRKDMEEWINIIKTIQQGEIYKTYTSGNITPT
DQQGSANAVALYSGPIHGMILC
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_5|176_aa
MQSITELLSKKSPELEDLENSQPIHIAKPKKVCPAENIKENGRMTSRGSSEASKAAERGS
EARDAAVAIIGPKNVGPRGRGTASLVPEGGVVSWVPEAGAVPQRISLELWFLSGKRELKA
DIQLPHISGPFKGSPLLSCPTGNIGSTSRARSLGVSQGITVRPAITHGGTAGPIQP
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_6|1057_aa
MLVELQSSDLGSRKVLGAQSAKAKSMSLPQDSPSSILITLELSSVLMYNKHYNHKAIPEH
IPAAENNPFLVGMHCWYSSYSHRTQHCNVCRESIPALSRDAIICEVCKVKSHRLCALRAS
KDCKWNTLSITDDLLLPADEVNMPHQWVEGNMPVSSQCAVCHESCGSYQRLQDFRCLWCN
STVHDDCRRRFSKECCFRSHRSSVIPPTALSDPKGDGQLVVSSDFWNLDWSSACSCPLLI
FINSKSGDHQGIVFLRKFKQYLNPSQVFDLLKGGPEAGLSMFKNFARFRILVCGGDGSVS
WVLSLIDAFGLHEKCQLAVIPLGTGNDLARVLGWGAFWNKSKSPLDILNRVEQASVRILD
RWSVMIRETPRQTPLLKGQVEMDVPRFEAAAIQHLESAATELNKILKAKYPTEMIIATRF
LCSAVEDFVVDIVKAWGQIKQNNTAIVSVILKSDLMYDRLSVLIDVLAEEAAATSAEKSA
TEYADSSKADRKPFIPQIDHIAKCKLELATKAQSLQKSLKLIIFQVEQALDEESRQTISV
KNFSSTFFLEDDPEDINQTSPRRRSRRGTLSSISSLKSEDLDNLNLDHLHFTPESIRFKE
KCVMNNYFGIGLDAKISLDFNTRRDEHPGQYNSRLKNKMWYGLLGTKELLQRSYRKLEER
VHLECDGETISLPNLQGIVVLNITSYAGGINFWGSNTATTEYEAPAIDDGKLEVVAIFGS
VQMAMSRIINLHHHRIAQCHEVMITIDGEEGIPVQVDGEAWIQRPGLIKIRYKNAAQMLT
RDRDFENSMKMWEYKHTEIQAAPQPQLDFQDSQESLSDEEYAQMQHLARLAENLISKLND
LSKIHQHVSVLMGSVNASANILNDIFYGQDSGNEMGAASCIPIETLSRNDAVDVTFSLKG
LYDDTTAFLDEKLLRSAEDETALQSALDAMNKEFKKLSEIDWMNPIFVPEEKSSDTDSRS
LRLKIKFPKLGKKKVEEERKPKSGQSVQSFIAWLPPRDLKPPLEMFVGMVMMKREAIWES
AVSDTKPYLGQGNLWHRRHREDEAEGDDPLTPSRSQL
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_7|491_aa
MCPLKALIEKAEHFSLICKAGQLPKCVRIDNASVLKGTGDIHRNLAEAVSVFVAESQLVR
TVLDVADTPVKLVDLYLMKAVCKKDKLQLLGATAFMIAAKFEEHNSPRVDDFVYICDDNY
QRSEVLSMEINILNVLKCDINIPIAYHFLRRYARCIHTNMKTLTLSRYICEMTLQEYHYV
QEKASKLAAASLLLALYMKKLGYWVPFLEHYSGYSISELHPLVRQLNKLLTFSSYDSLKA
VYYKYSHPVFFEVAKIPALDMLKLEEILNCDCFYTKSFDFGDVSIKVQSENRNHSSYFEQ
RKFNAGNWLLKDRRAKKSHTQCEASVILRAGGTVAKRWCHQTPRCQGYQAGFGTSDQLGP
MRDHPESQLGFRVLLGNPFSIMIIPDGDSWFALLLSSLTCHRDLHVACLELGWPPKPTCP
GENSCAN Output http://genes.mit.edu/cgi-bin/genscanw_py.cgi
PFPPPFNSLYAEHRAFLEKGTHNCLPPIVLTPEAVGGSSNVSAFSSPLFFWPGIAYPTNS
SESATKDTTKP
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_8|1025_aa
MLKNFKKGFKGDYGVTMTPGKLRTLCEIDWPALEVGWPSEGSLDRSLVSKVWHRGTCKPG
HPDQFPYIDSWLQLVLDPPQWLRGQAAAVLVAKGQLVKEGCHSTHRGKSAPKVLSEPTPE
ESWQELVPAVPPPYREEGLPIPEPTAPPLPPDIHTPRPPRVDKRRSEAMGETPPLAAHLR
PKTEIQMPVREQQYTGVDEDRHMAATKWLEEHVLANYQNPQEYRRIQLPGTGHQWDLNKG
PDMERLRQYHAAWIEGLKKEAQKDTNVNKVSEVIQGKEESPAQFYERLCEAYRMYTPFDP
DSPENQRMINMALVKVWKISGENCRNRLGLQEPTVRITIGGKDIKFVVDTGTEHSVVTTL
VTPLSKETIDIIRATGVSTKQAFCLPWTFSVGGHEIVHQFLYMPDCPLPLLGRDLLSKLR
ATISFTKQGSLQLNLPETGVIMALMVTREKEWRLFLTEPGQEIKPALAKRWPRIWADDNP
PGLVVNQDPVLIEVKPGAQPIRQKQYPVPREALEGIQVHLRHLKAFGIIVPCQSPWNLPF
LPVPKPGTKDYQPVQDLCLVNQATVTLHPTVPNLYTLLGLLQAEDSWFTCLDLKDAFFSI
RLAPESQKLFAFQWEDPESDLGLLQYTDDLLLGHSTAVGCTKGTDALLWHLEDCGYKVSK
KKAQICRQQVHYLGFTIQKGERSLESERKQVICSLPEPKTRRQGYRGGDWEPFEWGPLQQ
QAFFPGLPDLTKPFTLYVSEREKMAVGVLTQTVGPWPRPVVYLSKQLDGVSKVWPPCLRA
LAATALLAQEADKLTLGQNLNIKAPHAVVTLMKTKGHHWLTNARLTKYQSLLCENPHITT
EVCNTLNPATLLLVSESLVEHNCVEVLDSVYSSRPDLWDQPWASVDWELYMDGSSFINPQ
GERCAGYVMVTLDAVIEAKPLPQGTSAQKAELIALTRALELSEGKTVNIYIDSRYAFLTL
QVHGTLYKEKGLLNSGGKDIKYQQEILQLLEAVWKPQKVAVMHCRGHQRASISVALGSSR
ADSEA
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_9|218_aa
MHETTHPGQESLEKLLGRYFYISQLPALAKAVTQQQCVTCQQHNARQGPTVPPSIQAYGA
APFEDLQVDFTEMPKCGGDHVWMKDWNVAPLRPQWKGPQTVILTTPKAVKGQCNPLELVI
TNPLDSRWKKGERVALGISGARLNPRVNILVRGEVYKRSPEPMFQTFYDELHVPVPEIPG
KTRNLFLQLAEHVAQSLNVTSCYVCGGIVMGDQWPWQA
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_10|1069_aa
MEKPLILDISTTSKTPNTEEASLFRKPLVLKEEPTIEDETLINKSLSLKKCSNHEEVSLL
EKLQPLQEESDSDDAFVIEPMTFKKTHKTEEAAITKKTLSLKKKMCASQRKQSCQEESLA
VQDVNMEEDSFFMESMSFKKKPKTEESIPTHKLSSLKKKCTIYGKICHFRKPPVLQTTIC
GAMSSIKKPTTEKETLFQELSVLQEKHTTEHEMSILKKSLALQKTNFKEDSLVKESLAFK
KKPSTEEAIMMPVILKEQCMTEGKRSRLKPLVLQEITSGEKSLIMKPLSIKEKPSTEKES
FSQEPSALQKKHTTQEEVSILKEPSSLLKSPTEESPFDEALAFTKKCTIEEAPPTKKPLI
LKRKHATQGTMSHLKKPLILQTTSGEKSLIKEPLPFKEEKVSLKKKCTTQEMMSICPELL
DFQDMIGEDKNSFFMEPMSFRKNPTTEETVLTKTSLSLQEKKITQGKMSHLKKPLVLQKI
TSEEESFYKKLLPFKMKSTTEEKFLSQEPSALKEKHTTLQEVSLSKESLAIQEKATTEEE
FSQELFSLHVKHTNKSGSLFQEALVLQEKTDAEEDSLKNLLALQEKSTMEEESLINKLLA
LKEELSAEAATNIQTQLSLKKKSTSHGKVFFLKKQLALNETINEEEFLNKQPLALEGYPS
IAEGETLFKKLLAMQEEPSIEKEAVLKEPTIDTEAHFKEPLALQEEPSTEKEAVLKEPSV
DTEAHFKETLALQEKPSIEQEALFKRHSALWEKPSTEKETIFKESLDLQEKPSIKKETLL
KKPLALKMSTINEAVLFEDMIALNEKPTTGKELSFKEPLALQESPTYKEDTFLKTLLVPQ
VGTSPNVSSTAPESITSKSSIATMTSVGKSGTINEAFLFEDMITLNEKPTTGKELSFKEP
LALQESPTCKEDTFLETFLIPQIGTSPYVFSTTPESITEKSSIATMTSVGKSRTTTESSA
CESASDKPVSPQAKGTPKEITPREDIDEDSSDPSFNPMYAKEIFSYMKEREIEETAQSHE
GENSCAN Output http://genes.mit.edu/cgi-bin/genscanw_py.cgi
QFILTDYMNRQIEITSDMRAILVDWLVEVQTGKAVVSEELEECSNQKHL
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_11|91_aa
MAKKLKTLKKKIDEWLTRITNAEKSLKDLMELKTMARELRDECTSLSKQGDQLEERVSAT
EDEMNEMKHEEKFREKEYKEMNKASKKYGTM
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_12|230_aa
MKAIETQKSLQKINESRSWFFEKINKIDRPLARIIKKKTEKNQIDAIKNDKGDITTDPTE
IQTTIREYYKHLYTNKLEILEEMDKFFDTYTLPRLNQEEVESLNRQITDSEIEAIINSLP
TKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTK
KENFRPISLMNIDAKILNKILAKGIQQHSKKLIHHDQVGFIPGMQGWFNI
>/tmp/03_14_10-19:06:14.fasta|GENSCAN_predicted_peptide_13|105_aa
PTPVSVENLSSMKPVPGAKKTGENCQTKISPSSLQESPSSLQGALKKRSAFEDLTNASQC
QPVQPKKEANKEFVKVVSKKINRNTHALGLAKKNKRNLKCNSVPV
Back to GENSCAN
GENSCAN Output http://genes.mit.edu/cgi-bin/genscanw_py.cgi
ORF Finder (Open Reading Frame
Finder)
PubMed Entrez BLAST OMIM Taxonomy Structure
gi|289450895|gb|AC239396.3|
View 1 GenBank Redraw 300
Frame from to Length
-3 148138..151098 2961
+2 125021..125869 849
+2 123776..124510 735
+2 121985..122689 705
+1 160522..161214 693
+1 161266..161952 687
+1 159484..160137 654
+3 119625..120209 585
-1 104661..105191 531
+1 158923..159420 498
+2 148154..148621 468
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