A SEMINAR ON:

SEROLOGICAL REACTION

Submitted By:
Mr.SUJIT J. TAMHANE

For The Year 2006-07

ACKNOWLEDGEMENT
I am greatful to our trustee Mr. Tekchand
Shah for giving me the opporturnity to do this
project work.
I am greatful to our respected principle Dr.
(Mrs)Sudha Rathod for giving me
encouragement & inspiration.
I am also thankful to Mrs. Smita mahajan
(Project Incharge) for giving me valuable
guidance in the preparation of this project
work.
I thanks to all faculty members, librarian, and
my friends for giving me helps to me
complete this project.
 DEFINATION OF
SEROLOGICAL
REACTIONS:

FEATURES OF ANTIGEN-
ANTIBODY REACTION :
FOLLOWING TYPES OF
SEROLOGICAL REACTION :

1.NEUTRALISATION REACTION

2.PRESIPITAION REACTION

3.AGLUTINATION REACTION

4.FIOCULATION REACTION

5.COMPLIMENT FIXATION
1.NEUTRALIZATION REACTIONS :

2.PRECIPITIN REACTIONS :
3.FLOCCULATION REACTIONS
:
4.AGGLUTINATION REACTIONS
:
5.COMPLEMENT FIXATION
TEST:
ENZYME – LINKED
IMMUNOSORBENT ASSAY
(ELISA) :
ELISA PLATE :
WESTERN BLOT :
ELECTROPHORESIS
IMMUNOELECTROPHORESIS
:

IMMUNOFLUORESCENCE :
Antigen detection :Agar-gel
immunodiffusion (AGID)
Counterimmunoelectrophoresis
(CIEP)
Passive haemagglutination (PHA)
Immunofluorescence (IF)
Immunoperoxidase staining (IPS)
Immunocapture ELISA
Electron microscopy (EM)
Polymerase chain reaction (PCR)
1.AGAR-GEL
IMMUNODIFFUSION (AGID) :
2.COUNTERIMMUNOELECTROP
HORESIS(CIEP) :

RESULT
3.IMMUNOFLUORESCENCE (IF) :
METHOD:
DIRECT IMMUNOFLUORESCENCE
A

C
INDIRECT
IMMUNOFLUORESCENCE :
4.ELECTRON MICROSCOPY (EM) :

METHODS

ASSESSMENT OF RESULT

5.POLYMERASE CHAIN REACTION

(PCR) :