(B. Sc.

Biochemistry)
Fourth Semester 2005
Full Marks: 100
Subject: Biochem 222 (Energetic & Metabolism I) Pass Marks: 45
Time: 3 hours
Candidates are required to give their answers in their own words as far as practicable. The
figure in the margin indicates full marks.
Group (A) Long Questions (Any three) ×14 = 42)
(3×
1. Explain Bioenergetics and thermodynamics. Describe the role of ATP in energy transfer
during metabolism.
2. Describe the various steps of regulation of TCA cycle.
3. Describe pentose phosphate pathway.
4. What is oxidative phosphorylation? Describe chemiosmotic model for ATP synthesis.

Group (B) Short Questions: (Any six) ×7 = 42)

(6×
1. What are the steps involved in glycolysis?
2. Write short note on regulation of glycolysis
3. Calculate total yield of ATP when a molecule of glucose is completely oxidized to
Carbon dioxide and water.
4. Describe the structure of the enzyme Pyruvate Dehydrogenase complex.
5. List out the various steps of Kreb's cycle.
6. Describe the modes of electron transfer in biological oxidation-reduction reaction.
7. Explain different types of electron acceptor involved in oxidative phosphorylation.
8. Describe various energy rich compounds other than ATP.
Group (C) Very Short Question: (Any eight) ×8 = 16)
(2×

1. Define metabolism.
2. What do you mean by reducing equivalent?
3. How does oxidation reduction potential play role in electron transfer mechanism?
4. How does entropy change occur when a glucose molecule is degraded completely?
5. What do you mean by phosphorylation potential?
6. What is the importance of isocitrate lyase?
7. How does a acetate molecule oxidize?
8. What is mass action ratio in ETS? How does it affect electron transfer and ATP
synthesis?
9. What will be the fate of pyruvate in the medium with lactic acid bacteria ?
10. Explain Pyruvate dehydrogenase complex.
11. How does a molecule of fructose metabolize?
12. Give a schematic representation of Malate- Aspartate Shuttle.

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