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  • Preparation of 0.1M Phosphate Buffers at pH 5.8-8.0

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    vanadispyridine
    891 vues1 page
    The document provides instructions for preparing 0.1M potassium phosphate buffer and 0.1M sodium phosphate buffer at pH values ranging from 5.8 to 8.0. For each buffer and pH, it specifies the volumes of 1M potassium or sodium phosphate stock solutions needed to achieve the desired pH when diluted to 1 liter with distilled water. The buffers are intended for use at 25°C.

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    The document provides instructions for preparing 0.1M potassium phosphate buffer and 0.1M sodium phosphate buffer at pH values ranging from 5.8 to 8.0. For each buffer and pH, it specifies the volumes of 1M potassium or sodium phosphate stock solutions needed to achieve the desired pH when diluted to 1 liter with distilled water. The buffers are intended for use at 25°C.

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    891 vues1 page

    Preparation of 0.1M Phosphate Buffers at pH 5.8-8.0

    Transféré par

    vanadispyridine
    The document provides instructions for preparing 0.1M potassium phosphate buffer and 0.1M sodium phosphate buffer at pH values ranging from 5.8 to 8.0. For each buffer and pH, it specifies the volumes of 1M potassium or sodium phosphate stock solutions needed to achieve the desired pH when diluted to 1 liter with distilled water. The buffers are intended for use at 25°C.

    Droits d'auteur :

    Attribution Non-Commercial (BY-NC)
    Téléchargez comme PDF, TXT ou lisez en ligne sur Scribd
    Signaler comme contenu inapproprié
    sur 1

    Preparation of 0.

    1M Potassium
    Phosphate Buffer at 25C
    Desired
    pH
    5.8
    6.0
    6.2
    6.4
    6.6
    6.8
    7.0
    7.2
    7.4
    7.6
    7.8
    8.0

    Volume of 1M
    K2HPO4 (mL)

    Volume of 1M
    KH2PO4 (mL)

    8.5
    13.2
    19.2
    27.8
    38.1
    49.7
    61.5
    71.7
    80.2
    86.6
    90.8
    94.0

    91.5
    86.8
    80.8
    72.2
    61.9
    50.3
    38.5
    28.3
    19.8
    13.4
    9.2
    6.0

    Dilute the combined 1M stock solution to 1 litre with distilled H2O.

    Preparation of 0.1M Sodium


    Phosphate Buffer at 25C
    Desired
    pH
    5.8
    6.0
    6.2
    6.4
    6.6
    6.8
    7.0
    7.2
    7.4
    7.6
    7.8
    8.0

    Volume of 1M
    Na2HPO4 (mL)

    Volume of 1M
    NaH2PO4 (mL)

    7.9
    12.0
    17.8
    25.5
    35.2
    46.3
    57.7
    68.4
    77.4
    84.5
    89.6
    93.2

    92.1
    88.0
    82.2
    74.5
    64.8
    53.7
    42.3
    31.6
    22.6
    15.5
    10.4
    6.8

    Dilute the combined 1M stock solution to 1 litre with distilled H2O.

    Fisher Scientific UK, Bishop Meadow Road, Loughborough,


    Leicestershire LE11 5RG
    Tel: +44 01509 231166

    Fax: +44 01509 231893

    E-mail: lifesci@fisher.co.uk

    www.fisher.co.uk

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