V{Fe 3+} (ml) Sample # [Fe 3+] (M) 1/[Fe 3+] (L/N) A400 A455 A500

0 0 0
1 5 1 0 558.66 0.19 0.39 0.29
5 2 0 0.19 0.39 0.29
5 3 0 0.19 0.39 0.29
STDev= 3.06E-04 4.00E-04 2.65E-04
2 6 1 0 465.55 0.22 0.45 0.33
6 2 0 0.22 0.45 0.33
6 3 0 0.22 0.45 0.33
STDev= 3.06E-04 1.53E-04 3.00E-04
3 7 1 0 399.04 0.24 0.5 0.37
7 2 0 0.24 0.5 0.37
7 3 0 0.24 0.5 0.37
STDev= 1.20E-03 2.17E-03 1.88E-03
4 8 1 0 349.16 0.26 0.54 0.4
8 2 0 0.26 0.54 0.4
8 3 0 0.26 0.54 0.4
STDev= 1.56E-03 2.14E-03 2.77E-03
5 9 1 0 310.37 0.28 0.58 0.43
9 2 0 0.28 0.59 0.43
9 3 0 0.29 0.58 0.43
STDev= 1.04E-03 1.94E-03 7.57E-04
6 10 1 0 279.33 0.3 0.63 0.46
10 2 0 0.31 0.63 0.47
10 3 0 0.3 0.62 0.46
STDev= 1.51E-03 2.48E-03 2.69E-03
7 11 1 0 253.94 0.32 0.67 0.49
11 2 0 0.32 0.67 0.5
11 3 0 0.32 0.67 0.49
STDev= 9.87E-04 1.04E-03 2.01E-03
8 12 1 0 232.77 0.34 0.7 0.52
12 2 0 0.34 0.71 0.52
12 3 0 0.35 0.71 0.53
STDev= 3.05E-03 3.93E-03 3.63E-03
9 13 1 0 214.87 0.36 0.73 0.54
13 2 0 0.36 0.73 0.54
13 3 0 0.36 0.73 0.54
STDev= 5.29E-04 1.76E-03 1.00E-03
10 14 1 0.01 199.52 0.37 0.75 0.56
14 2 0.01 0.36 0.75 0.55
14 3 0.01 0.36 0.75 0.55
STDev= 1.67E-03 2.06E-03 3.03E-03
[SCN-] / A400 [SCN-] / A455 [SCN-] / A500

0 0 0
0 0 0
0 0 0

0 0 0
0 0 0
0 0 0

0 0 0
0 0 0
0 0 0

0 0 0
0 0 0
0 0 0

0 0 0
0 0 0
0 0 0

0 0 0
0 0 0
0 0 0

0 0 0
0 0 0
0 0 0

0 0 0
0 0 0
0 0 0

0 0 0
0 0 0
0 0 0

0 0 0
0 0 0
0 0 0

[SCN-] stock (M) [Fe 3+] stock

0 0.0179 M
 [SCN-]Total/Aλ vs. 1/[Fe 3+]total
0 0.8
0 0.7
[SCN-] / A400
0 0.6
[SCN-] / A455
[SCN-]Total/Aλ

Absorbance ()
0 [SCN-] / A500 0.5
0 Linear regression, 0.4
[SCN-] / A400
0 Linear regression, 0.3
[SCN-] / A455
0 0.2
Linear regression,
0 [SCN-] / A500 0.1
0 0
0 100 200 300 400 500 600 0
1/[Fe 3+]total
 [Fe 3+] vs Absorbance
0.8

0.7

0.6
Absorbance ()

0.5
A400
0.4
A455
0.3 A500
0.2

0.1

0
0 0 0 0 0 0 0 0 0 0 0.01 0.01
[Fe 3+] (M)