1

' ' 3
. E.coli E.coli . E.coli
[ ( 0 20 40 60 90 120 ) 1 720 984 1872 ' ] 10 5 2 828 1032 1928 [ 1 138 140 160 188 285 484

"

1'
' ] 10 6 2 167 171 170 197 284 600

2'
(' ) 0 20 40 60 90 120 N(10-5) [ " /' ]
1.55*108 2.02*108 3.80*108

10 5 774 1008 1900

10 6 153 156 165 193 285 542

N(10-6) [ " /' ]

3.05*108 3.11*108 3.30*108 3.85*108 5.69*108 1.08*109

[ " /' ]
2.30*10
8 8 8

Log N
8.3615 8.4087 8.5502 8.5855

O.D nm660 0.179 0.212 0.300 0.396 0.490

2.56*10 3.55*10 3.85*10 5.69*10 1.08*10

8.7551

0.579
9.035

:
(O.D. = 0.007)

O.D.

: :10 5
720 + 828 = 774 ' 2 ml

t=0 ) N(10-5)

: ( " 0.5
774 105 = 1.54 108 ' 0.5 ml

E.coli
E.coli
10 4
1 114 4 2 93 1 1 29 0

3'
10-6
4 -5 -6

10-5
2 14 0 1

10
2

10

10

A [ " /' ]

Log N

O.D 660 nm

A
60 90 120

5 2

2 0

103.5 2.5

21.5 0

3.5 1

1.3*108 8.114 4.5*106 6.649 6.8*105 5.8346

0.05 0.048 0.024

E.coli
( ) 1 10 4 2 1 118 239 10-5 2 156 254 1 20 24 10-6 2 10 4

10-5

4'
10-6

B [ " /' ]

Log N

O.D nm660

B
60 90 120

1.26*108 18 23 137 246.5 19 23.5 3.27*10

7

8.101 7.5145 7.3711

0.042 0.051 0.051

2.35*107

E.coli
( )
1

'
10-6
2 1 2

5'
10-5 10-6

10 4
2 1

10-5

10 4

C [ " /' ]

Log N

O.D nm660

C
60

90 120

0 2

0 1

0 0

0 0

1 0

0 0

0 1.5

0 0

0.5 0

1.21*108 3.33*105 1*104

8.084 5.5229 4

0.032 0.040 0.028

: . . ' , '

O.D 660 nm 0.700 0.600 0.500 0.400 0.300 0.200 0.100 0.000 8.3 8.4 8.5 8.6 8.7

y = 0.6152x - 4.9415

8.8

8.9

9.1 log N

NB . :
y = 0.6152 x 4.9415

E.coli ' "

:1'

t=60min A,B,C
t=60min
' ml

log N
8.114 8.101 8.084

:6'

O.D (660nm ) 0.05 0.042 0.032 A B 55 ' C :

1.3 *108 1.26*108 1.21*108

. (O.D.A = 0.010, O.D.B = 0.008, O.D.C = 0.006) : O.D. . A,B,C A,B,C :A

y = 0.6152 x 4.9415 0.05 = 0.6152 x 4.9415

1'

" (.t=60min) , .

t=60min : O.D.

x = log N = 8.114 N = 10 x = 108.114 = 1.3 108 '

ml

O.D (660 nm) 0.70 0.60 0.50 0.40 0.30 0.20 0.10 0.00 0 20 40 60 80

55

100

120

140 t [min]

E .coli 55 ' . " ,

log N

' ,

E . coli

2'
55

10 8 6 4 2 0 0 20 40 60 80 100 120 140

t[min]

37 .

-E.coli ' .

' .

, 55 '

:3'

E.coli
" ' : E .coli " . . , . .

E.coli
" " . . . .3 '

E.coli
. 3+2 , " " RNA " . 4' . "

S50 ,

E.coli
) , " . . ' ' : . 30 40 0 c .

'
E.coli " ( 55o c . 5' ,

'

6 : 100= t min 6.3 10 8 3.1 10 6 3.1 10 7 1 10 5 .log .3 ' : log N

8.8 6.5 7.5 5

' 100

A B 55 ' C . " 100 log N

"

( ( 55
log N

55 ( (

) )

10 9 8 7 6 5 4 3 2 1 0 0 20 40 60 80 100 120 140

t[min]

y = -0.0122x + 8.7564 y = -0.038x + 10.284 y = -0.0681x + 11.996

E.coli

55

, '

:3'

"

. .

end point .

" E. coli : , . ( . .

) " " " : . .

. .

:'

E. coli End point 10-5 10 6 10 7 10 8 10 9 2 [ "

'

1 , ,

1.46 109 1 109 1 109

78 12 2 0

67 8 0 0

2 E. coli End point 10-5 10 6 10 7 10 8 10 9 [ " '

6.5 109 1.1 109 1.5 109

2 , 70 10 2 0 :
-7

1 , 60 12 1 0

. . : "

10

'

1' " 0.1 "

"

0.1ml 1 1ml x x= 1 1ml = 10 0.1ml

' . " , " : 10

1*108

N=

10 = 1 108cfu 107 ml

:
. 10 9 , end point , . .2 .1

:'
,

NA

'

NA

NA NA

:
. . . , , . NA . . , , .4 , .1 .2 .3

NA NA

.5 .6

:
:' ,( end point . ) 2

4 :' . " , . , . . . " , , , , NA . + .4 .5 , . .2 .3 , .1

5
. :

Bacillus subtillis Penicillin Eritromycin Gentamicin Sulfadiazine Ampicillin

Chloramphenicol

Staphylococcus aureus

Bacillus cereus

E. coli
0.2 1.1 2.2 1.4 1.8 2.2 -

Sarcina lutea
1.5 2.7 1.3 1.6 4 3.1 3.1 1.4 1.6 2.7 1.8 1.1 4.6 3.3 3.2 1.6

Serratia 1.7 1.7 1.6 3 1.4 1.6 1.6 1.6 1.4 2.9 0.7 1.8

Proteus vulgaris
0.4 0.9 1.2 1.1 1 1.1 0.8 0.7 1.2

Tetracycline Neomycin

0.5 3.2 1.9 2.4 2 3.2 3.4 1.7

3.5 2 1.6 2.1 2.8 3.5 1.8

0.5 1.9 1.9 1.6 3 2.2 2.7 1

2 1.45 2.7 2.3 2.6 0.8

1.5 1.9 2.2 2.8 1.3

3 2.9 2.2 2.8 1.8

. "

"" "

Penicillin

Ampicillin

Sulfadiazine

Chloramphenicol

Neomycin

Gentamicin

Eritromycin

Tetracycline

" Bacillus subtillis Sarcina lutea Proteus vulgaris Bacillus cereus

Staphylococcus aureus

Serratia

E. coli

E. coli

.E. coli :

time (min) 0
'

20
0.101 0.116 0.109 0.118 0.102 0.122 0.073 0.099 0.081 0.100

40
0.131 0.163 0.167 0.118 0.104 0.163 0.076 0.104 0.180 0.120

60
0.185 0.229 0.221 0.140 0.116 0.243 0.136 0.111 0.216 0.164

80
0.280 0.303 0.313 0.153 0.141 0.350 0.226 0.134 0.242 0.214

100
0.336 0.365 0.360 0.154 0.147 0.400 0.300 0.159 0.260 0.271

120
0.400 0.430 0.410 0.160 0.140 0.500 0.380 0.190 0.360 0.360

160
0.420 0.457 0.417 0.156 0.134 0.518 0.400 0.220 0.333 0.390

140
0.463 0.494 0.412 0.156 0.125 0.617 0.435 0.269 0.363 0.454

180
0.500 0.520 0.416 0.157 0.130 0.642 0.435 0.303 0.280 0.481

K
0.0185 0.0194 0.0185 0.006 0.011 0.020 0.019 0.012 0.009 0.017

T
53.93 51.58 54.14 156.18 91.08 49.47 53.35 83.88 113.09 58.80

1 2 3 4 5 6 7 8 9 10

0.075 0.080 0.060 0.070 0.068 0.065 0.049 0.049 0.049 0.082

K
.

11 '

:
N log t (log N t log N 0 ) N0 1 K= = ;T = 0.301 t 0.301 t 1 K [min ]
min

:(
Nt 0.400 log N0 0.185 K= = 0.301 t min1 0.301 (120 60) log

)'

120

'

60

= 0.0202min 1
min 1

:K
1 1 T= = 49.47 min K 0.02

log

: . " 4.5 C1 V1 = C2 (V2 + V1 ) 2% " 0.5 :1'

0.02 0.5ml = C2 (0.5 + 4.5) ml C2 = 0.02 0.5ml = 0.002 = 0.2% 5ml

:

O.D(660nm) 0.600
0.2% 1

0.500 0.400 0.300 0.200 0.100 0.000 0 20

0.08% 2 0.04% 3 4

40

60

80

100

120

140

160

180

t[mIn]

:1'

. . .

(4

: ) 13 4' 13

lag
3' .

2 1

120

O.D. (660 nm)

0.500 0.450 0.400 0.350 0.300 0.250 0.200 0.150 0.100 0.050 0.000 0 0.05 0.1 0.15

0.2

0.25
[ %]

2'

0.04% .

0.08%
3'

k [ 1/min]

0.0250 0.0200 0.0150 0.0100 0.0050 0.0000 0 0.04 0.08 0.2 [%]

K-

E.coli
. 0.04%

3'

9 . ,6' " E.coli

O.D. (660 nm) 0.700 0.600 0.500 0.400 0.300 0.200 0.100 0.000 0 20 40 60 80 100 120 140 160 180 -6 t[min]

E.coli
,

4'
.

O.D. (660 nm) 0.180 0.160 0.140 0.120 0.100 0.080 0.060 0.040 0.020 0.000 0 20 40 60 80 100 120 140 160 180 t[min]

-4 -5

5'

10

O.D. (660 nm) 0.500 0.400 0.300 0.200 0.100 0.000 0 20 40 60 80 100 120 140 160 180 t[min] -7 -8

6'

40 .

O.D. (660 nm)

0.600 0.500 0.400 0.300 0.200 0.100 0.000 0 20 40 60 80

37 25

-1 -9

t[min]

100

120

140

160

180

7'

o 37C

o . 25C

120

o 25C

' .

'

11

O.D. ( 660 nm )

0.600 0.500 0.400 0.300 0.200 0.100 0.000 0 20 40 60

-1 - 10

80

100

120

140

160

180 t[min]

8'

.E.coli . ,3 ' 1' 120 , , , " .( . , , " ) .

1'

, " 0.1% . . . . , , , . , ,5' , ' 4' , 3' 2' 3'

, .

E.coli

. . . , ,

12 . 6' . . . . 30 40 ' o 37C ' 40 .

lag ,

E.coli
. 7' . . , , , . , , , , , 8' . . .
o 25C

E.coli

. , :