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Ann DEL ROSARIO, Kariza Fabiola DELA CRUZ, Cathleen May DELA CRUZ, Jose Miguel GROUP 2 B1
At
the end of the conference the students would be able to: Discuss the catabolism of heme
Differentiate the different types of jaundice Explain the occurrence of jaundice in the ff:
Physiologic jaundice of the newborn Hemolytic Jaundice Polycythemia Blood Extravasation Defects in conjugation Breast milk jaundice Obstruction of the biliary tract
Jaundice,
also known as icterus, refers to the yellowish discoloration of the skin or sclera yellow pigments begin to deposit in the sclera of the eyes and in the skin when the bilirubin concentration in the plasma is elevated (hyperbilirubinemia)
The
the
derived when
About
80% comes from the destruction of heme-containing proteins (myoglobin, cytochromes) and catabolism of heme
large
Globin
Within
Formation
Glycine activated by pyridoxal phosphate(coenzyme) Reaction of Glycine with Succinyl CoA(from Kreb s Cycle) by ALA synthase forms -aminolevulinic acid (ALA) Occurs in the mitochondria
Formation
of Porphobilinogen(PBG)
Two molecules of ALA are condensed by the enzyme ALA dehydratase to form porphobilinogen Occurs in the cytosol sensitive to inhibition by heavy metal ions
Formation of Hydroxymethylbilane(HMB) condensation of four porphobilinogens produces the linear tetrapyrrole, hydroxymethylbilane by PBG deaminase Formation of Uroporhyrinogen III Hydroxymethylbilane is isomerized and cyclized by uroporphyrinogen III synthase to produce the asymmetric uroporphyrinogen III by Uroporphyrinogen synthase
Formation of Protoporphyrinogen IX
Coproporphyrinogen III enters the mitochondria Two proprionate side chains are decarboxylated to vinyl groups, generating Protoporphyrinogen IX Enzyme is coproporphyrinogen oxydase
Formation of Protoporphyrin IX
Formation of Heme
introduction of iron (as Fe+2) into protoporphyrin IX occurs randomly Enhanced by the enzyme ferrochelatase Like ALA ferrochelatase is inhibited by lead
Prehepatic
Hepatic
Post Hepatic
Test
used for estimating conjugated as well as unconjugated bilirubin levels in plasma diazo reagent (diazotized sulphanilic acid) is added to conjugated bilirubin a reddish brown coloration is obtained
Erlichs
Unconjugated
bilirubin, which is waterinsoluble, has to be dissolved in methanol to complete the Van den Berg reaction is added to the solution to direct the presence of unconjugated bilirubin
A reddish violet coloration obtained upon adding = presence of unconjugated bilirubina indirect Van den Berg reaction/ indirect bilirubin
Methanol
Hyperbilirubinemia
bilirubin levels in the blood are elevated above the normal range of 0.3-1 mg/dL
Jaundice
rise
in the water-insoluble, unconjugated or indirect-reacting bilirubin in the blood not usually accompanied by a rise in urinary bilirubin
Urinary
bilirubin rise if the water-soluble, conjugated or direct-reacting bilirubin is in excess in the blood
Types
of hyperbilirubinemia:
(1)
Retention hyperbilirubinemia
(2)
Regurgitation hyperbilirubinemia
Unconjugated
hyperbilirubinemia
retention of unconjugated bilirubin in the blood production of bilirubin exceeds the capacity of the liver for its uptake, conjugation and biliary excretion may lead to acholuric jaundice with unconjugated hyperbilirubinemia
usually With
increased urinary and fecal urobilinogens produced in excess from bilirubin may also arise from the crossing of the blood brain barrier by nonpolar unconjugated bilirubin (kernicterus)
Encephalopathy
Unconjugated
In
liver dysfunctions, liver cells fail to remove unconjugated bilirubin from blood and to conjugate and excrete it in bile increases serum unconjugated bilirubin producing jaundice.
This
Conjugated
hyperbilirubinemia
Conjugated
or direct-reacting bilirubin may rise in the serum may appear in the urine, producing choluric jaundice
bilirubin
Regurgitation
of conjugated bilirubin into the blood from the biliary duct system may occur
obstructed by a tumor or gallstone (regurgitation hyperbilirubinemia)
May
Hepatocellular impaired:
disease
Excretion
= most affected
choluric
urine
urobilinogen levels
reduction in hepatic reuptake of the urobilinogen urobilinogen is eliminated in larger amounts in urine
urine
urobilinogen decrease
reduced excretion of bilirubin into the intestine with consequent reduction in the intestinal urobilinogen synthesis
For
the same reason, a rising urinary urobilinogen level in a patient with jaundice under treatment is a good sign
Type 1/B1
Type 2/B2
Pre-hepatic
- The pathology is occurring prior to the liver. - The pathology is located within the liver.
Hepatic
Post-hepatic
also
called physiologic jaundice of the newborn 5 mg/dl of hyperbilirubinemia present normally may be
appears within 2 to 5 days of birth and last for about a week yellowing of the skin and other tissues of a newborn infant
In
the fetus, bilirubin is removed from circulation by the placenta. birth, the newborn has to suddenly excrete its own bilirubin but its hepatic conjugation of bilirubin is still inadequate due to reduced UDP-glucuronyltransferase activity. As a result, hyperbilirubenimia occurs.
At
can
be reduced by phototherapy
Exposure
of skin to white or blue lights causes photoisomerization of bilirubin to water-soluble bilirubin excreted in bile without requiring any conjugation
rapidly
Bilirubin
levels that deviate from the normal range and requiring intervention would be defined as pathological jaundice. of jaundice within 24 hours
Appearance
Increase
peak
levels above the expected normal range of clinical jaundice beyond 2 weeks bilirubin (dark urine staining the
presence
conjugated
clothes)
unconjugated hyperbilirubinemia develops after the first week and continues up to the sixth week of life Yellowing of the skin and sclera (white part of the eye) 3-alpha-20-beta pregnanediol uridine diphosphoglucuronic acid (UDPGA) glucuronyl transferase Phototherapy
Hemo
excess breakdown of red blood cells. rupture of red cells is followed by the release of hemoglobin Increase in unconjugated bilirubin Level
The
also
called Plethora
hyperbilirubinemia, in which there are too many red blood cells in the blood circulation Polycythemia vera Secondary polycythemia reddish-purple coloring, respiratory distress, and low blood sugar are the symptoms of Polycythemia
Deep
leakage of blood from a vessel into tissues surrounding it occur in injuries or burns or allergic reaction
can
After
bleeding has started, the bruise continues to deepen in color until bleeding stops. Bruising of the skin develops
biliary obstruction refers to the blockage of any duct occurs when bilirubin cannot reach the intestinal area gallstones, malignancy, infection, and biliary cirrhosis pale stools conjugated bilirubin
Increase
rare autosomal recessive disorders are characterized by genetic defects of hepatic bilirubin-conjugating enzymes.
results from the genetic deficiency of the bilirubin-UDP glucuronyl transferase for converting bilirubin monoglucuronide unconjugated or indirect-reacting bilirubin rises in the serumk high unconjugated bilirubin in the serum damages the corpus striatum and other cerebral ganglia, leading to toxic encephalopathy
results from the genetic deficiency of the hepatic glucuronyl transferase for glucuronidating bilirubin Unconjugated or indirect-reacting bilirubin also rises in the serum Jaundice is milder, not necessarily fatal
Gilberts disease
may result from rare autosomal dominant genetic deficiencies in the hepatic uptake of unconjugated bilirubin from blood or in the hepatic bilirubin-UDP glucuronyl transferase benign congenital jaundice with a mild unconjugated hyperbilirubinemia
Dubin-Johnson Syndrome
rare
congenital jaundice also called the chronic idiopathic jaundice an autosomal recessive trait genetic deficiency of the liver in secreting conjugated bilirubin leads to its reflux into the blood and the consequent conjugated hyperbilirubinemia