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Biology 301
Lecture #6
Roosevelt University
Biology 301
Cellular and Molecular Biology
Spring 2002
Overall outline
The Central Dogma
Nucleotide Chemistry
Nucleic Acid Structure
Nucleic Acid Function
Ogan Gurel, MD
Biology 301
Lecture #6
Roosevelt University
Nucleotide Chemistry
Synthesis of Polymers
Breakdown of Polymers
Phosphoanhydrides & Phosphoesters
Chemistry of Phospho-derivatives
The structures of nucleotides and polynucleotides
Ogan Gurel, MD
Biology 301
Lecture #6
Roosevelt University
Synthesis of Polymers
Ogan Gurel, MD
Biology 301
Lecture #6
Breakdown of Polymers
Roosevelt University
Ogan Gurel, MD
Phosphoesters
Phosphoanhydrides are
common in ATP and other
“high-energy compounds.”
Phosphodiesters compose the
backbone of DNA and RNA.
Ogan Gurel, MD
derivatives
Adenosine triphosphate
(ATP) is a
phosphoanhydride
composed of an adenine
base, a ribose sugar and a
triphosphate group.
Phosphoanhydrides are
“High-energy” compounds.
High-energy is not an
intrinsic property of the
bond per se, but rather a
relative energy between
the phosphoanhydride and
its highly stable hydrolysis
products.
Ogan Gurel, MD
and polynucleotides
Ogan Gurel, MD
Biology 301
Lecture #6
Roosevelt University
Structure Determination
Roosevelt University
Ogan Gurel, MD
Biology 301
Lecture #6
DNA Structure
Roosevelt University
DOUBLE HELIX
{
OO
P
H O O 1. Xray fiber diffraction
H H 2. Structure of nucleotides
3. Chargaff’ s R u l e s ( G = C , A = T )
H O H
O CH2 Base Base CH2 O
{
H O O
H P
H O O
H H
H H
{ O
P
O H
H O H
O O CH2 Base Base CH2 O
H O H
{
H
O O H
P
O O
4. Suggested possible mechanism for REPLICATION
Proteins usually have a hydrophobic interior and a hydrophilic exterior. Describe how this
paradigm also applies to DNA.
Ogan Gurel, MD
Biology 301
Lecture #6
Roosevelt University
Chromatin Structure