GMR INSTITUTE OF TECHNOLOGY-RAJAM DEPARTMENT OF CHEMICALL ENGINEERING Bio Chemical Engineering Assignment-2 1. Define and describe Enzyme immobilization.

Give its advantages and disadvantages. 2. Enlist various immobilization methods. 3. Explain production of high fructose syrups from corn starch with neat flow sheet. 4. Give the significance of Damkohler number with respect to immobilized enzyme kinetics. 5. Explain diffusion effects in a porous immobilized enzyme matrix. 6. Describe monod chemostat model? 7. Explain growth cycle phases for batch cultivation. 8. A) Growth of filamentous organism means growth in size. Explain. B) Give a note on thermal death kinetics of cells and spores. 9. Consider an organism which follows the monod equation where max = 0.5h-1and Ks = 2gm/lt. In a continuous perfectly mixed vessel at steady state with no cell death, If S0 = 50g/lt, YX/S = 1.0 gcell/g sub. a) What dilution rate D will give cell concentration x=0. b) What dilution rate D will give the maximum total rate of cell production c) At this dilution rate D calculate cell concentration X, Substrate concentration s for sterile feed. 10. A strain of mold was grown in a batch culture on glucose and the following data were obtained. Time hr Cell conc. gm/lt Glucose conc. gm/lt 0 1.25 100 9 2.45 97 16 5.1 90.4 23 10.5 76.9 30 22 48.1 34 33 20.6 36 37.5 9.38 40 41 0.63

a) Calculate the maximum specific growth b) Calculate apparent growth yield c) What maximum cell concentration could one expect if 150gm of glucose were used with same size of inoculum?

19-01-2012

Submission date: 27-01-2012