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  • Inhibition

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    Sari Shara
    25 vues17 pages
    1. Enzyme inhibitors can be reversible or irreversible, with irreversible inhibitors permanently modifying the target enzyme. 2. The mechanism of inhibition can be determined by comparing inhibited and uninhibited reaction kinetics, categorizing inhibition as competitive, uncompetitive, or noncompetitive. 3. Competitive inhibitors compete with the substrate for binding to the active site, while noncompetitive inhibitors bind elsewhere and induce conformational changes, and uncompetitive inhibitors bind the enzyme-substrate complex.

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    1. Enzyme inhibitors can be reversible or irreversible, with irreversible inhibitors permanently modifying the target enzyme. 2. The mechanism of inhibition can be determined by comparing inhibited and uninhibited reaction kinetics, categorizing inhibition as competitive, uncompetitive, or noncompetitive. 3. Competitive inhibitors compete with the substrate for binding to the active site, while noncompetitive inhibitors bind elsewhere and induce conformational changes, and uncompetitive inhibitors bind the enzyme-substrate complex.

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    25 vues17 pages

    Inhibition

    Transféré par

    Sari Shara
    1. Enzyme inhibitors can be reversible or irreversible, with irreversible inhibitors permanently modifying the target enzyme. 2. The mechanism of inhibition can be determined by comparing inhibited and uninhibited reaction kinetics, categorizing inhibition as competitive, uncompetitive, or noncompetitive. 3. Competitive inhibitors compete with the substrate for binding to the active site, while noncompetitive inhibitors bind elsewhere and induce conformational changes, and uncompetitive inhibitors bind the enzyme-substrate complex.

    Droits d'auteur :

    Attribution Non-Commercial (BY-NC)
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    sur 17

    INHIBITION

    INHIBITION
    Mostly enzyme inhibitors act reversibly , they do not cause any permanent changes in the enzyme. However, there are also irreversible inhibitors that permanently modify the target enzyme.

    INHIBITION
    The mechanism of action of an ihibitor its inhibiton type can be determined by comparing the kinetics of the inhibited and uninhibited reactions. Based of the different kinetics , inhibition can typed to :
    Competitive inhibitors Uncompetitive inhibitors Noncompetitive inhitors

    TYPES OF INHIBITION

    TYPES OF INHIBITION

    Competitive inhibition
    There is competition between the substrate S , and the inhibitors I for the enzyme.
    If an inhibiting substance can bind at enzyme active site. Enzyme that binded inhibitors can not bind the substrate or the other way

    KINETICS

    Non-competitive inhibition
    The inhibitors is persumed not to bind to an active site on the enzyme.
    But rather to bind at some other site. This complex formation involve some changes confromation of the enzyme. Makes it impossible for the substrate to bind in active site.

    TYPES OF INHIBITION

    Uncompetitive inhibition
    Inhibitors combines reversibly with the enzyme-substrate complex after it forms. Its assumed that this complex is so stable that it does not lead to formation of the expected product. If this were not so, this case would reduce to a special case of noncompetitive inhibition.

    KINETICS

    COMPETITIVE

    NONCOMPETITIVE

    UNCOMPETITIVE

    Conclusion
    This leads to a decrease in the rate of the reaction not only because some substrate is consumed, but also because of the decrease in the effective concentration of the enzyme

    HOMEWORK
    Sketch a Lineweaver Burke plot for this enzyme in the presence and absence of inhibitor and report values for the table below given the following equations : Vmax app Kmax app V max Km
    No inhibitor inhibitor y = 1.52x + 1.51 y = 1.58x + 4.27 0.662 1.006 0.233 0.370

    What the type of inhibitor, justify your respon and explain what about the mode of inhibitor binding.

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