m1

m2
v2
v1
Primer ID pico mole req.pic mole total vol. req.Vol
LEC Water
ROS-F
164
10
100
6.1
93.9
Oxid-F
161
10
100
6.2
93.8
Delta-F
164
10
100
6.1
93.9
PIP2-F
164
10
100
6.1
93.9
lea5-F
166
10
100
6.0
94.0
Photo-F
165
10
100
6.1
93.9
Rbcsib-F
163
10
100
6.1
93.9
GhTPS-F
163
10
100
6.2
93.8
MPK-7
163
10
100
6.1
93.9
Ghsp-26
162
10
100
6.2
93.8
ABA2-F
162
10
100
6.2
93.8
Guni-F
165
10
100
6.1
93.9
Gapdh-F
147
10
100
6.8
93.2
ROS-R
160
10
100
6.3
93.7
Oxid-R
167
10
100
6.0
94.0
Delta-R
164
10
100
6.1
93.9
PIP2-R
165
10
100
6.1
93.9
lea5-R
167
10
100
6.0
94.0
Photo-R
164
10
100
6.1
93.9
Rbcsib-R
166
10
100
6.0
94.0
GhTPS-R
164
10
100
6.1
93.9
MPK7-R
162
10
100
6.2
93.8
Ghsp26-R
165
10
100
6.1
93.9
ABA2-R
164
10
100
6.1
93.9
Guni-R
165
10
100
6.0
94.0
Gapdh-R
147
10
100
6.8
93.2
HspcembF
133
10
100
7.5
92.5
HspcembR
130
10
100
7.7
92.3
Real-F
162
10
100
6.2
93.8
Real-R
164
10
100
6.1
93.9
GAPDH-F
162
10
100
6.2
93.8
GAPDH-R
165
10
100
6.1
93.9