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SlnA l- Wtichofthe following organismscan useRNA as its genetic material? A- Bacterium B- Virus C- Human t< D- Plant E- None of the above
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is 2. Which of the following enz)rmes usedfor making new strandsof DNA? A. Restrictionendonuclease B- RNA polymerase ,/ C- DNA polymerase ( D- Ligase E- None of the abc ''e

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is/areNOlf true formitosis? Z. Which of the following statements place in all typesof cells F A. It takes daughtercells that are identicai I B. It generates occursbetweenhomologouspairsof chromosomes process recombination of C. The
D; AandC E. BandC is/areFALSE for the human genome? 4. Which of the following statement(s) {

A. B. C. D. E.

Most of the DNA code for proteinsp Most genescontain introns-f Exonsdefine rbgionsof DNA which will not codefor proteins ? AandB AandC

Which of the following processinvolves forrnation of hydrogen bondsbetweenDNA and RNA? A. Replication B. Translation C. Transcription D. Splicing E. None of the above of 6. Which of the f-ollowingtypesof RNA is/areinvolvedin the process translation? A, Ribosomal RNA ,.2 /, B. TransferRNA RNA ' C- Messenger , D- Ribosomal RNA and transfer RfiA RNA E. RibosomaiRNA, trapsferRNA and rnessenger

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13.Recombinationduring meiosisis importantfor creating A. mutations B. gene diversity C. chromosomestabilify D. better genes E- longer chromosomes Questionsl4 to 19 refer to the following paragraph: _iy

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Kevin, aged 30, doesNOT developkidney cancerdespitethat one of its alleles gene for T carries a rnutation as depicted above. Prrrrlr.t. 14.What is the modeof suchmutations? A. Inversion B- Insertion C- Deletion D. Duplication E. {Jnknown

r q Rt r 6 r h f \ t q Cq - n G A qn p b " u ' A q F 6 ( o h c o 6 n f ll

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15-Kevin's wife is free from this disorderanddiagnostic testsconfirmedthat both her iilleles T haveno suchmutations.What is the chanceof their childrengetting the

C. 5OY" D. 75yo E. lA0yo

disegg:. in the future\ T A.Oo 1 B. 2s%o 1

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A- 250A B. 500A C. 75o/o
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E. Not enoughinformationto predict it

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is/are TRUE for the synthesis of proteins in qukaryotes.?. 7. Which statement(s) A. This is a decodingprocessmediatedby H-bonding between:tRNA and mRNA B- Proteins can be modified after translation 1 . C. IRNA are derived from transcriptionof its own gene{-. D. Aa ndB E. A,BandC 8 . Translationis a processof making A. DNA B. mRNA C. rRNA D. IRNA E. Protein

9. If a doqblg:stranded,Bryl has a cytosinecomposition of l5%o,what is the composition of its adenines?

A. 15Yo

B. zOYo C. 25Yo D. 35Yo

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E. Not enoughinformation to determine

10.If the codiag of sequence a DNA is 5'...GG(ccdrr{a""1.S,*at I

uu rr('bPu r r ur uB Ds r lus r r es r ur r r . 5. c r r u r r d r s {) corresponding sequencefor its.template?;' i _ n c i r { t
-r-o! \ [,

A. B. C. D. E.

5 '- .-GGGCCCTTTA A A ---3' i, : - i- i' i' ' ' i; / 5'...GGGCCCUUUAAA...3' 5'. ..TTTAJ{AGGGCCC...3' 5 '...UUUA A A GGGCCC...3' None of the above

A i rr
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6A 6 ccc TTTAAn
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From the Questionl0 above, for what is the correspondingsequence its mRNA? A. 5'...GGGCCCUUUAAA...3' 3' (cc Goc. AA;P-V!1tr g B. 5'. -.TTTAAAGGGCCC...3' C. 5'.. -IJTruAAAGGGCCC...3' 6(( i .c'-\'''.*1AFF D. 5'...AAATTTCCCGGG. -.3' E. None of the above

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12.The processof exchanging two regionsof hornologouschromosomes alsoknown is

AS

A. B. C. D. E.

mutation translocation cross-over transposition None of the above

17. Which'of the following descriptionsBEST describesthe role or properties ofprotein T? A. Wildqpe protein T is highly soluble B. Mutant protein T is highly soluble C. Mutant protein T causesformation of cancersonly when its wildtype counterpart is absentin cells D. Mutant protein T causesformation of cancerseven when its wildtype counterpart is present cells in E. None of the abovedescription is correct Refer to the genetic codon table provided in Appendix, and assumethat AAA represents of the codons in the gene,what effect would sucha mutation have on one the protein it encodes? h0 A- It replacesone amino acid with anothertype B- It lengthensthe ptotein ,,lAh C. It shortensttre protein ti r-. .._ D. It doesnot causeany changeto the protein at E. No protein is expressed all Wo|Y * '19. GeneT is madeup of 3 exons and2 introns with their length indicated below. \ Exon l:267 nucleotides rA \.\: , Intron l: 1003 nucleotides / t bi Exon 2:603 nucleotides \V-'-' Intron 2:3000 lgqleotides -A \\nt Exon 3:l26hucleotides

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translation, what is Assuming that all nucleotidesin the 3 exons are usedin subsequent the total length of the protein encodedby gene T? t cu'O' -'Z{'',,11';lr;d'VA. 133aminoacids B. 313 aminoacids qqb -- 3)2 C. 331 aminoacids -4D. 669 amino acids z <_r\1 \ --)il r t E. 996 amino acids {

Xc-

':L I (Td'f oCrv' t

20. Which of the following descriptionsfor the DNA skucture is FALSE? A. It is double-stranded with anti-parallglhelix -I B. It is a polyrner of nucleotides linked from one nucleotideto the next by phosphodiester linkage{' C. The polymer carriesa net negativechargedue to the chargeson its bases D . Aa n dB E. Ban dC

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Which of the followine human bhromosomes is the smallest? 'JtrilF-L 'tt^if A. Chromosome I B. Chromosome 5 C- Chromosome 15 D. Chrornogome 18 E. Chromosome 22

22.Which of the following statements below correctlydescribe(s) organization the of DNA in various species? A. Viral genomesare very compactand can utilize different frames for synthesisof different proteins T B. Humangenomemainly consists ofnon-codingsequence {

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rouna ineukaryott";f:*f;*"-' i-.

E_A,B and c

23. Which of the following orqrnization? (< sign denotessmallerthan) ( Tissue< cell ( chromosome nucleus< DNA < nucleotide "{ ' p-Cell < tissue< nucleus< chromosome DNA < nucleotide < 'Ila Tissue< nucleus< cell < chromosome DNA < nucleotide < l"E') Nuct"otide < chromosome DNA ( nucleus< cell < tissue < E. None of the abovehas the correct order
t

V hol in^ prr( py..D{*'L ./ w\Aa' vc{erlfo -/ statements below correctly describesthe relative order of

tl'*\' \Y,ni ,^' hr \-.!,, r"^1u

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are | 24.How many types of chromosomes therein a healthy humanrygs$r? v A.2z B. 23

c. 24
D. 46 E. None [.-t 15 ' -J Differentpropertiesof amino acidsaredue to differencesin their -l A. charges B. sizes -i C. centralcarbon atoms F D. Aa ndB E. Aa ndC

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26. More than 100,000proteinscanstill be generated from the 35,000genes predicted in genomebecause the human of A- altemativeRNA splicing B. modificationof proteinsaftertheir synthesis C. geneduplications D . AandB E. Ba ndC

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/Zl.Which of the following statement(s)is/are lBtiE for human cells? A. All human cells can replicate y- toralCfXlp I B. All somatic cells are haploi{F daff;a C. All germ cells are diploid )r lV$t;a D- All somatic cells have identical geneticmake-up T E- All germ cells have identical geneticmake-up 7 28. Successful development biotechnologyindustryrequires of A. extensivemanpower B. soundfunding/financial support C- relwnt topics of research D. Aan dB E. A,BandC

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29. Amino acids in prottiins are linked up through chemical bonds termed A. hydrogenbonds B. phosphodiester linkages C. peptidebonds D. ionic interactions E. hydrophobic interactions 30. Cenhal dogma of molecularbiology describes A. how genetic information canbe transmittedin cells B. how proteins can fold to proper shapes C- how DNA.mutat.ionsaffect our life D. how genes aremddified E. how cells undergoadaptation

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1. Which statementis not true about DNA replication?

ontyJ lt proceedsin a 5' to 3' direction . ^ {'"a. fasier than the other strand{ fwtu\t .ltr*l e)!,i,!g t dr( One strand,ofDNA is replicated ]lke Replieationoccurs as each base is pairedwith anotherexaetfry' it F base pairs are held together by hydrogenbonds Complementary a cell, of strand the doublehelix is foundin eachdaughter 2 . Becauseone original processis called the replication A. B. C. D. A. BC. D. Proofreading Semi- conservative Freeingof DNA Red'undant bacteriawere
+ t'ttitod\e/

3- Transformatisn of bacteria was shown to occur when injectedinto the mice and the mice
'Sfu{Dth-

A. Live, smooth;died to,"'.gh hqqv\lix B. Live, rough;didn't die 9-Live, smoothand died, smooth;died lD. /Live, rough and dead, smooth;died

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4. Which is not a major functionof the genetic material? . A. Stories information-{ B. Catalyzes chemical feactions C. Replieatesitself \ D- Undergoesmutatibnrf -

5. DNA was provento be the transfsrmingsubstancewhen only the enzymes could inhibit transfor.mation. A. B. C. D. Proteinase RNase DNase Lipase

C.
..- \--r

with G, whichof theseis true? 6. Knowingthat A bin{s wjth T and C blind,s

y( A+-r= c-c c. n=c,i=c

D. A=C, T=G

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would 7. lf a speciescontains 23"/oA in its DNA, what is the pereentageof G.:it eontain? A- 234/" &_ 46%

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c. 27%
D. 25o/a

carriesthe geneticinformation needed 8- Whichof the classesof RNA molecules of for the construction a protein?
a

A. B. C. D.

RibosomalRNA TransferRNA RNA Messe.nger Primarym RNA,transcript

9. Which is the process by which a protein is constructed? A. B. C. D. Translation Transcripfon Transforinb{pn Transposifion*
(\

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10.Whichof the followingnucleotide bases is only found in RNA, not DNA? A. B. C. D. (G) Guanine Cytosine(C) Uracil(U) Thymine(T)

11.Whichof the classesof RNAmoleculesis neverfound in the cytoplasm? A. B. C. D. Ribosomal RNA rf t TransferRNA J MessengerRNA Pre- RNA

12. A nonsgllg [i1ta!io-n one that is

A. B. C. of acidsfrom thepointof D . Produces completely inco\g0tsequence arnino a

themutation the end of thg$rotein J to

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13. Whicfl of these Is NOT a fomi of a point mutation? A. B. C. D' Nonsense rnutatlon)' MlssensemtrtationY Sifentmuta i:on \ Frameshift rnuta{ion - cvrq1*'rtt

14. Whatis the functionof a,vector geneticengineering? in A. B. C. D. CutDNA into many fragments DNA intoa new cell Carry of Linktogether newlyjoinedfragments DNA Makemillionsof copiesof a specificsegmentof DNA

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enzyme in genetic engineering? 15. What is,the functicn of a restriction A B. C. D. Cut DNA into many fragments :' CarryDNA into a new cett of Linkiogethernewlyjoinedfragment DNA Makemillionsof copiesof a specificsegmentof DNA

16.Whatis the functionof DNA ligasein geneticengineering? A. B. C. D. Cut DNA into rnanyfraEments GarryDNA into a new cell Linktogether.newly joined fragmentof DNA Makemillions of copies of aspedfic segmentof DNA

17.Whatis the polymerasechainreaction? _ I A. B. C. D. . A reaction which duplicatesproteins A reaction which produeesmanyRNA molecules A reactionwhich producesmanyDNA fragments A reactionwhichis like DNAtranscription

lB.Whichis not a cycleof PCR? A. Denaturatign/ L'enaturailP C. ElongatioV D. Translation

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D- f\ilHearililgr

inhenited triction site polymorphismsiare

D. By alwaYsfollowingthe father detectedby 20.A change in DNA that affectsa restrictionsi!:e,1s , A. PhenotYPiccharacteristies B. Genome sequencing . C. Difierence in restrictlonfragments D. Cloning diseasesdre 21. Gene-tic A. B. C. D. Birth defects Very common EasYto detect Mosily untreatable

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i3 22.Down's sYndrome causedbY A. B. C. D. DNA deletions DNA insertions Foint mutations Excess chromosomes

of 23.With the advancement molecularbiotogy, A. B. [h b. lt is possibleto detect all genetic diseases lt is possibleto diagnoseall chrornosomedisorders tt is possibleto predicta birth defect tt is possibleto correcta birth defect

completethe sentence'The humangenome 24.Choosethe best statementto to project is a Project A. Study hum{ns B. Analyze all humanchromosomes in G. Determinethe DhlA sequences all human chromosomes D. Diagnosehumandiseases
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25.PCR is a rnolecular biology;technique which can A. B. C. D. Ampliff genes lsolategenes Amplifuproteins lsolate pr,oteins

celtanemiais a 26.Sickle 6 " A., Geneticdisease B. Acquireddisease C. Female-associateddlseSe D. A male- associated diseff;e

27.Genetherapy a therapyusing is A. Viruses B. Vaccines genes C. Engineered D. ChemicalDrugs 28.A|DS a is , A. B. C. D. Geneticdisease Viral disease Bacterialdisease Environrnental disease

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29.Microbes be useful'inbioremediation can because 'tt,\,'' / v/ A. They can break down many organiccompounds B. They can break down rnany plants C. They canbreak down many animals D. They can break down many nutrients

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because 30.Molecular biology can helpthe environment .\rf n. 'lt can createnew molecules . .-'. B. ..ltcan release CO 3 : ' '.fr- lt can release'CO2 the behind, environment the - D. lt can helpus understand physiology

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lfy engineeredmierobes AB. CD. Always grow betterthan natural ones Are alwaysuseful Are alwayswidely spread around the world the loealenvironment Can ahange

when is 32.Gene therapy possible A. B. C. D. / causingthe diseasehas been identified The gene(s) 'i' The diseaseis causedby a singleEene defect / fg(tors The diseaseis causedby multipleEenes and environmental// \ (A) + (B) snowis made by A. B. C. D. Dead ice-formingbacteria Live ice-forming-bacteria Bacteriain which the ice- nucleationgene was removed material Non- bacteria

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engineeredmicrobes that can break down crude oil 34.GeneticallyA. B. C. DAre not yet in existence in Are already use Are planned Cannotbe created

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the 35.Who is holding patentfor PCR? A. B. C. D. Kary Mullis Du Pont Cetus Noneof the above

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36.The patentfiled by StanleyCohen of Stanford Universityand Herbert Boyerof UC in 1974was for the inventionof A. B. C, D. DNA te${nology / Recombinapt Humanin$1lin \ Human grriwthhorlnohe None of the above

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vectors,such as _ into cells usingbiological 37.DNA can be transferred and means, such as -, or directphysical -

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GENES AND SOCIETY SEMESTERI2OOI/2002 l. A. B. C. D. E.

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Which ofthe following samplesmiglrtcontain DN.A? a dinosaurfossil a drop of pond water iilthe backyard a chunk of a tressskin a cooked)6sh dedund \Xh all of the above

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Bacterium A. is a single cell of organism B. is a prokaryote t/ C. contains acellwall v/ -D. can be beneficial'to human \/' E- all of the above 3. A. B. C. D. E.

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Southern blot is a technique used in DNA fingerprinting J' geneshansfer PCR transformation \./ . gel electrophoreg{ \\

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4- A single nucleotide Aglgllgn3t the 6e amino codon of the hemoglobin gene was found. What will happen to the hemoglobin protein produced? A. a diffgrettt p ill be produced,which has no hemoglobin "o*plet"ly stt{e ctll qutctei^.-rtt*.

tuffi\

B. a shortenedprotein will be prodXced C. no protein will bs prob{ced D. a normal hemoglobin {vitt be pro}dced E. a slightly different hemoglobinwill be produced 5. A. B. C. DE. 6. A. B. C. D. E. DNA fingerprinting is a specific DNA patternof a person the DNA on a person's finger capturedby camera can be seeneasily with a microscope none of the above of Which of the following is not a constituent a nucleotide? ribose \ -/ phosphategroup y' bases r/ amino iicids none ofthe above
-)

.F j A

7A. B-. C. D. E.

J-aines WhBou and F'ranei$Hok diseovered the struotureof Pennicillirr the str-uctureof DIIA the structure of Insulirl the structure of E. Coli the structure of }trf,V

8. DNA cloning requires the use of rnolecutrar scissorscalied A. RNA polyrr,rerises "B. DNA ployr,r,rerises Restriction endonucleases r C. D. Helicases E. Recombinase

/9 . A. B. C. D. E.

Which statement below appliesto the techniqueof PCR? it is very tedious it doesnot requireamplification ot requires the knowledge'ofrestriction enzymecutting sites it was co-inventedby Buyer and Cohe\',-', ,', , i', , None of the above

10.Which statementis true? A. most prokaryotic genes contain interveningsequencecalled in(ns e B. virus is a tlpe of bacteriurn that affect humans F C. r ni tf ^. ,-,.$ D- lNt"*'+"r,"""t1t*;e*"ti" Uy@sjlption in the nucleus tr +vqt{.Y}Pt -!^r.. ' pnocrue Most proteins are generated *Fin, . E. Transforrnationrefers to the linkipTf trvo DNA moleculesinto one F
-/ 11. A changein DNA that affectsa restriction enzyme recognition site is detectedby A. phenotypic characteristics B. genomesequencing C. difference in reshiction fragrnents D- cloning E. hansformation //:-\\: '{,'.12--iekle anemia a caused cell is by l . l: -t:r r bNa mutation .../ . 'i.l.. -i.i l;,? '\.a... B- Malnutrition

c.

D. E. Ribosemutation

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C. 1970s

7A. B-. C. DE.

JarnesW+Bou and F'raricie griok d:iscovered the struotureof Pennicillir* the skucture of DNA the structure of Insulirl the structure o,fE. Colf, the structure ef ltrf,V

8. DNA cloning requires thc use of rnolecutrar scissorscalied A. RNA potyr,r,rerises .8. DNA ployn-erises Restriction endonucleases "C. D. Helicases E. Recombinase /9 . A. B. C. D. E. Wbich statement below appliesto the techniqueof PCR? it is very tedious it doesnot requireamplification ot requires the knowledge ofrestriction enzymecutting sites it was co-invented Buyer and Cohe\'','''r.:, , ,r , by None of the above

tr

l0 . Which statement true? is A. most prokaryotic genes contain interveningsequencecalled inSns e B. virus is a type of bacteriurn that affect humans F C. RNAcanalsofunctionaseeneticinformationstoragematerialT. .u r -.i .rr Ah,rrr,, ipt'- '+'-< "' D- Most proteins are generatedby franscriptionin the nucleus tr +vqb{(Y , 1';;i; ' ''-tr' E. Transformationrefers to the linkip o-ftrvo DNA moleculesinto one F J 11. A changein DNA that affectsa restriction enzyme recognition site is detectedby A. phenotypic characteristics B. genomesequencing C. difference in reskiction fragrnents D- cloning ( E. hansformation by i, .lp.S,iAAesell anemiais a caused ' ,.i.. A. DNA mutation .../ B-'Malnutrition

i'i'n{'

C. D. E. Ribosemutation

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( ( 13.|.ecombinant DNA technology is first developedin the \ \ A. ,zl950s

t96os C. 197Os

D. 1980s E. 1990s / 14. Hybridization can occur r DNA strandso{Y A- betweentwo complementary single strandednualeic acids B. betweentw'o cornplementary C. betweentwo .o-pl"*"ntary single strandedXWn o\V D. betweena protein and a single strandedDNA E. betweena plasmid DNA and a chromosomalDNA only

15 .Which statement(s)is/are'notcor,rectfor the synthesis of proteins in eulqgrotes? A. this is a decodingprocessmediatedby H-bonding between tRNA and mRNA T B. proteins can be modified after translation 1 by C. maturedproteins are generated splicin-gsegmentsof peptidesafter translation 7 D. AandB E. None of the above / t0- Which of the following applicationsneed to involve the useof restriction digests of DNA? \AITR by PCR'p < ,/ Mapping "{g"W" clonin$/ Gene AandB BandC

wt

17. Antibiotic selectionis an important step in genecloning in bacteriabecause DNA (containinginsert) from the parental A. it helpsdistinguishrecombinant plasmid relegated B. it allows selectionfor transformant(harboring the vector) whether the vector containsan insertif not C. it ensuresthat bacteriaused in researchis not released to the public strain ofbacteria to be isolated D. it allows new antibiotic-resistant n J) E- it is a cheap and easyway of obtaining a gene U qr is 18. Which of the following statement true for Sciuthernblot? A. it canbe performe! bV-lhemelhodsof RFLrff B. it requiresa labeledprolp J \ , C. it may or may not invslve gel eleg\(Phoresis Y\ D. itinvolveshybridiiang/ 1 E. BandD (i**'''-,'';''" cells 19. Protein synthesisin eukaryotic A. always starts from apartiiular amino acid { nrc\\;it*M" ! B. startsrandomly 6 few aminoacidsi C. canstart from a selected D. can start from 3 different aminoacids -1-

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E. doesnot start with a anrino aeid

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^ description the risk mutation on_hgr chromosome, X whatis the mostaccurate of . .. itt ',.,if I 11 -r,6rst^ tosusan'd/sop)GF:T.^Ei;J n,"1 rrr,-,ta-{r"1,, i{ ---ev11^ frrltv^, A. if hisfathliilso carried mutatfn,"tti*iiiai.Eutdl_g* tire diseaserc the ru -^.

Y\{ T \,

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inherited from his grandmothegz ,@\h" may get the disease \9t'tre may be a carrierfor the disease\-D, he may be a carrierfor the disease'butwill definitely not be sick F /'f'5 th" gly kind sf RNA witlr activity in the'nucleus F the only kind of RNA wittr aetiviry in cytoplasmf6-r,,rnfu[' ,

is theonly kind of RNA involved ,transe_ription iis theonly kind of RNA inv,olvedfr-nslal6n I nsnofthe answers correct is

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BC. D. E-

a hiplet of deoxyribonucleotides which is read fron 3' to 5' direction F a triplet otribonuchgtiges which is read from 5' to 3' directionf a triplet ofEnffior-iid=is which is read from 3' to 5' direction a triple of amino acidswhich rnakesup the polypeptidechain F

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23. I havetwo plants- with yellow andred flowers respectively.I do not know theirn genotlpes, but know that red.is.,:tlEi order to determine the exactgenetic makeup, I cross ttremand get 12 offsprings. I' :-----_--:.. . I A. ifthered:yellow ratio is 6:6, the yellow wd prb-bablyprne-breds theJed a vi il' and hybrid B. if the red:yellow ratio is 6:6, bsth were probably pure-breds p C . if I get I red and4 yellow, they were probably both hybrids (i-e. one yellow and one red gene in eachplant) D- if I get 8 red and4 yellow, ttiey were probably a red pure bred and a yellow hybrid E. if they were all red, the red was certainly pure bred,but I still don't know any {,, more about the yellow 24- Which of the following is/are required for transcription ' A. DNA polymerase \ f / B. A singleskand of DNA for use asa template ,,/ piece ofQNA, both are usedastempl C. A double stranded

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D. Free tsFhg"cleotides
E. A andD bre coFect

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25. The direcfion of information flow defined by the centraldogma is A. Protein ---+RNA --+ DNA

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RNA DNA RNA DNA

--+DNA --_.> protein -.> p:otein + RNA ----+ protein -+ DNA -> RNA -+ protein

26- Polymerascchain reaetion(PCR.)is very usefrrlbeoause A. it can cut DNA B. it is patented C. it can amplify DNA D. it is createdbyNobel Iaureate E. it can puriry DNA 27- A recombinantprotein is A. a protein which is a combinationof two other proteins B- a protein that is produced from bacteria C. a protein that is producedby geneticengineeringmethod D- a protein that is producedby virus E- not really a protein

t-

28. A biological vector is A- a vehicle which can be usedto ferry genesfrom one organisrn to another B. a vehicle which can be usedto ferry viruses ftom one organism to another C. a vehicle which can carryproteins into the nucleus D. a vehicle which can carrybiologieal weapons E. a containerwhich can storebiological specimens 29.Aggl""

.$

q r.Gl,F i' A. si7.-udrqccpc{ice:;g rn c i1 B. 5,T CGA GA GGTCA T3' 5',T CGA GA GGfCA T3'. . . . . ^ t I . . , I I ' l ra A I -^ r. r, ^ . uc'r ut" 9 rialdLc\ I C. 5'uccAcaccucll l'h ' , .tr ^ .i{ D. 5' AGCUGAGGUCUA 3 E. none of the above
30, Which of the followingprocesses involve tRNA ? L A. transcriptiM ,

$t' .' Iiln:.in.se;AGd b,,ti sene? iACCITCTFC this ' DNH irgJlil $4'p"nfj:{Pr" . rir.,rf )Itew^1u,k
^

F"dl"P I c.qdllgJgand 5' ATGIACCIICTCGA Which of thefollowingis the is 3'.

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fo it''iuedlu"q

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/ S\tt"tt bqlq\-q

T{ ' J '

B. DNA '"pr{}l( " C. Transfo.midoir.

D. Translation'-Y

E. tu\Asprifte

Qy

Ss+ F

GEM 1527 Quiz20.0212003 Semzr K

lWhich of the following is Not a constittent of,a nucleotide? A. Ribose / ./ Y B. Phospha;egroup C. Base '/ D .Primer / E. Carbon element ,'/ A. Southernblot is a techniquethat involves A. Nucleic acid hybridization B. DliIA ligation

b"0., nibcr, frr*pl.a1b

,/ D \-./ ,/

c. PcR.

'

D- Transformation E. Antibiotic selection 3-

.
h

genewas A singlenucleotide insertionat the6e amino acidcodonof thehemoglobin protein produced? fouff-$niit wiif nappen the hemoglobin to
A. BC. D. E. An hemoglobin protein containing an extra amino acid will beproduced. No protein will be produced. A normal hemoglobin will be produ(d A sliglrtly differenthemoglobin will be produced A cornpletely different hemoglobin will be producedwhich hasno hemoglobin function. /

4-

Gregor Mg!g4-an4 Thomas Morgan discovered: A. B. C. DEThe antibiotic PeniciVin DNA is the genetictfraieSf The gardenpea and frui$fly ' \ The law of heredity The chromoslQes

5.

DNA cloning requiresthe use of carriers called A. B. C. D. E. Virus Gene.gun Plasmid Antibiotic Template

.L.
'. I

" I tK{ - (Jir r { 1 r ' ,h U\ v '\l t ur

rr'f.r f Il( 11 tt

1 r. I rLTl

r ., '\-

bry Wtlicilei,sr'nq"t

P
78-

Te.tt

BIot? to thetegl]:iqucof Sotther,n

lh. B. C. D: E.

It is ateeh*ique basedon the plasmidX It requires FCR amplifioation It*oqtr-iied gel electrophoresis . ./ and Cohen { It wasico-i,nvented tsoyer' by None of the above

by Restriction Fragment Length Folymorpllisrn(Rl, PJ is generated A. B. C. D. E. Variations in ph Variations in chro Difference.in individualts blood,types Cloning : A, change in DNA sequencethat affects a restriction enzynrerecognition sit

The structureof DNA is determinedin A. B. C. D. Et95os 1960s 1970s 1920s 1930s lqEt Vwrt:*t ait?

tr*

9.

Hybridization can occur A. B. C. D. EDNA strands Ontybetween two complementary single strandednucleicacid Betweenany two complernentary Onlybetween two complementarysingle strandedRNA Between a protein and a single strandedDNA Between a plasmid DNAand a chrornosomalDNA

10.

Which statement(s) for is/are nelg9r,r99t the synthesisof proteins in eukaryotes? A. B. CD. EtRNA and mRNA -T This is decodingprocessmediatedby H-bonding between ' 'f Proteinscan be modified after translation t/ by Matured proteins are generated splicing segmentsofEgetr$es after translation F A andB None of the above

Antibiotic selection iS an imporrant step in gene eloning into a plasrnid because A- It helps to distinguish recombinant DNA(containing insert) from the parentalreligatedplasmid B. It allows selection f,ortransformant(harboring the vector) regari\ whethel the vector containsan insertor not C . It ensuresthat bacteriaused in researchis not releasedto the public D. It allows new antibiotic-resistantskain of bacteriato be isolated E. It is a way to keep the plasmid clean

6(9) Proteln s1'nthesisin eukaryoticcells

A. B. C. D. E.

J:\

&

Occurs in he cytoplasm',r/ a Always stop At a parlicular amino acid f- t\^1a.cr CrH rtdcn . $. "te-npf Can stop at a selectedfew amino acid p RequiresRNA polymeraseI Does not require rRNA I "$. J{K-ltnted r6e5*v& John's father had mophilia which is causedby a mutation on his X ohromosome,.rr What is the most accuratedescription of the risk of John's

,Y,t

r
)

I \

(''l

John's daughterwill get hemophilia inherited from John's fatherf . 9 John's daughterwill definitely be a carriei for hemophilia p .. D. If John'swife is a carrier,John's daughtermay be a carrier 1 "l9. John's daughterwill definitely be sick fr ;'g( ;y

$. 'd.

ff lunn's wife is fuFig5 John'sdaughterwill definitelygethemophilia F

tyi' xl'y

IRNA

{7\
-<\

r\wtu

A. Is the only kind of RNA producedin the nucleus P' B- Is the only kind of RNA produced in the cytoplasm p. C- Is the only kind of RNA involved in transcription F l
I

J-"t'rq )

t
\ \

Q a triplet of ribonucleotideswhich is read from the 3' to the 5' direction E. A triplet of amino acidswhich makesup the polypeptidechain

.7

.-4

(\

16.

You were glven two plants- with yellow flower/short body and purple fl'swer/tall body respectively.It is known that purple and - In order to deterr'nine exact genetic*ut the and got 24 offSprir,gs. A. If u got two types of plants,the ptrple/tall: purple/shortwith a ration of 12:12, -T then the purple/tallplani must be a purebreed in the colourtrait and a hybrid in { the heighttrait. B. If all wire purple/tall, both plants were pure breeds 1 C. If u got two types of plants,purple/tall: yellow/tall with a ratio of 12:12,then the purple/tall plant must be a pure breed in the height trait and a hybrid in the colour -; ' \ trait. D- If pulple/shortand yellow/tall plants appearedin equalnumbersasthe yellodshort and purple/tall plants, then the purple/tall plant must be a hybrid in both hait. I E. All of the abdveN r fi f Which of the following is/arerequired fsr PCR? A. DNA polymerase B- Primers -z' C- A piece of double shanded DNA as ternplates
D. Free deoxyribonucleotides E. All of the above -,/' (-v,^

17-

18.

The centraldogmaof molecularbiology dictatesthe informationflow as A- Protein -+RNA-+DNA B- RNA >DNA > protein +protein-->RNA C- DNA D- RNA -protein --|DNA E. DNA ____>RNA_>protein

19.

A virus is A. B. CDE. An organism which canlive independently F An organism which is a parasitethat lives inside a cell I . A DNA molecular which is very small F A RNA molecular which carries geneticinforrnation ? A container which cansorebiologicalspecimens f

.l -L' \rn
' r'
I ' a. j.:.,i-

, !.

r
' \

I li r
i

if

20.

A gene's'Jitivestrand 5' ,{TQgCQfCffpCA 3'- Which of the following is the is I \ by this ,"n"t _,'BNA gdffted g r ,, A. 5'AUGACCUCUCGA3'

. a{.gilrv.Je J+'-'$f.{t I

n{o c llucc[Hc "'

E. None of the above ^it, Which of the following is/areresultsgfDN+.single nucleotidemutation? A. Stickle cell anerHa '

e'RNff"?' CN,* \r
\f/

B. Down'ssyrdrfi
C. Turner's s5mdronie D. Huntinglon disease E. A&D. rWhichof the following statement tr,ue? id A. B. C. D.
I

v,

Introns only exist in prokaryotic gegres F In eukaryols, most of the time a mRNA can generatemultiple nrot-ei1s 'O (I) In bacteria, transcriptionand translation cannot occur simultaneously-pIn human cells, transcription from a Fg.rqfirst generatesa RNA that is different from mRNA found in the cytoplasm\ I ) E. In human cells, tRNA anainNe ur"it/d" directly in the cytoplasm F

Lxels{gur
23. 1r :
''i .'

A;{$!R A. B. C. D. E.

(variablenumber tandemrepeat}

t'

) Existin ouf ls unique in every indivi W;4 Is of a fixed length Differs onlybetween male and female Is essentialfor Southernblot

uNfrcl' Trrihj sor\'t

trYm

.'.'

',1

Questions24 to 30 refer to the following paragraph:

A-'

DiseaseMisknowntobe@,a",fiJ""grrentofthechromosornethat is responsible fur this disorder is localized on cg4qa!@I Jihere there is a cofunntafon from the sequence the coebst_rand of the gene M from: of

s'-.-cAch.4te@ryf*"i 3'to5'cAclcc4t&@{$.{ia,e.c -:'

Where the kiplet grouping indicate the readingframg suffer from this disorder althoughthey carry onl
gene. IVIr Lee and his wife both

Vtvt i*onlr
24-What ,d. B. C. D. E. 25is the name given to the different forms of a gene? Haploids Diploids Alleles Genotlpes Mutants
./

,fl

What is the mode of inheritancefor the disease? A. B. C. D. E.

whr+e A P d,iteaov

Autofmal, recessive \l \.., Autog{rnal,dominant I Sex-lirit recessive Sex.linlq d3rnlrr3lt .j Not enoughinformation on whether it is dominant or recessive

>(F x"

s l*

LJ.r' ._./

f\

,/

.v
/\

ii'p,

{Y I.t- t' itrT| c. 750h

26.

Wlren their child is born, what is the chance of him,/hergetting the disease?

x KI xJ"
7

ra ynys xo.f gP xo'i

X.r

D. 100% E. Not enoughinformation to predict that If theif child were to suffer from the same disorder,what is the chanceof him/her TFboring bo_th sppiespf the faulty genes?
-{-----__ __L

/ "rfg
F r ^r\ r'riVd K

Al25Yo

.,8. lz.g%" c. 66.7% D. 100%

E. Not'bnoughinformation to predictit

3\\u)< \> -

-"\

R.s

28.

Wha,ttypeof,mutatlon is this?
A.. BL. Di E. fnse,rti.ol* Dbletion Iransposrcron Pointrnutatio* Trar*stroeation

ft.{
29-

If M:. and Mrs- I eehad a healthy son,what is the ehancethat this son will have a diseased child? A. If this son marriesa healthywife, thenhis childrenwill all be healthy. J B. If this ssn married a diseasedwife who car,ries two copies of the mutated geDe, thenhis children will all be sick. f C. If this son marries a diseased wife who carries only one copy,of the mutaled gen6, thenhis children can be either healthyor sick T D. If this son marries a diseasedwife who carriesonly one copy of the mutatedgene, -lthenever5r new born child will have 5OYo chance1obe sick I \ E. All of the above possibilities If Mi- and Mrs. Lesshad a sick daughter who only carries one copy of the mutated gene,which of the following-statement true? is AB. C. D.

I 1, tY '1-1 ya
?.
30.

,v

1f

If this daughter marries a healthyhusband,thenher son will all be healthy. F If this daughter marries a diseased husband,the her son will all be sick F If this daughtermarriesa diseased husband, then50olo chanceher son will be sickT If this daughter marries a diseased husband,then 50olochanceher daughterwill be F . \ sick E. All the above possibilities
_/ ..Sat \t
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Process lfaUsrti{tfi_, X

vVff'A4rc\ Process

1-5 Questions belowrefer to the following diagramdepictingpart of the centraldogmaof molecular biology. Studythe diagramcarefully.

o
- f . r ." ;' ; f '1.In
ri r)

Z Process {WnSl*fbn
)

r)

't'
rl

I
I

'

7s\

))' --*J J,. -\--z

"/

which organisms theseproc6seslX -z){Utngplace? are A.Bacteria .' fJ J" B.Vi r us | \ {t

C.Playtdrotrnlralpelis dkcrTft' } D.Pro{aryo\es{S E.All o{p}abbve

/ 2. Which of the following statement(s) is/areNOT true for Process This process X? X takesplacein humansomaticcells. strandas thetemplate. {uses the antisense (involves oneof the enzymes calledRNA polymerase. on {determines the sequences mRNAs,rRNAs, andtRNAs. E. AII of the aboveare conect.

1 /\

Y? of below is/areTRUE for Process ,' [ 3.,/vvhich the statement(s) in a, tatl tatt^ \Xsynttresis of the new moleculeproceeds 3' to 5' direction oll{ B. Only the exonsget transcribed maturemRNAs in theprokaryoticcells. gulcnrg$v io y1rura DllA doublehelix molecule. in C.It canhappen all six framesof th-eTaffie maturemRNAs havea capanda polyA tail in the baclg!4!99!ls. XThe E. None of the aboveis true.

ta a
a

I --

4. Which of the following factorscontrolswhereand when Process shouldtake place? X ,A(Exon B. Special codonsnamelyTAA, TAG andTGA (referto the codontableon the lastpage) D. Activator and repressorproteins E. Noneof the above

fP)

5. Which of the following statement(s) is/areNOT true? A. Products from Process havea limited shelf-life.-fos.yr'{Ntttzyrah Y B. Products from Process Ztre further modified in all organismssuch as virus. 6tttg1e$c4yflo C. Process requires Z accurate H-bondpairing u, J D .Aan d B E.Ba n d C

6 Questions to l0 refer to the following paragraph: Disease is known to be an inheritablegeneticdisorder.The A chromosome is responsible this disorderis localizedat that for therpis a commonmutation frog the sequence the of

I{,Prgene iflrOr

ccccc....3'.

5' .. GGGGGATCCCCCTAillq.CC G-cc...S.fo S

TCC CCC TAA

Unfortunatety tiiiiiG trasalsobeendiagilpsed tiTe sufferingfrom the onsetof Disease with all symptorfrs A qpsociate1r&1th disease. the

tfrffi5fue{nlrfnd, fr?t.??;r' ;Fil.frf,$:,t #,n" F# ;,,""o" *.;',i#_ o,,,",

D. Autosomal, enoughinformationon whetherit is dominantor recessive. not E. Noneof the aboveis correct. 7. Whentheir sonis born, what is the chance him sufferingfrom the samedisease? of A. Atleast2io/o B. At least50% C. At least757o D. 100% E. Not enoughinformation to determine.

v/

fi* P'h|\"
C\ W F1*aa

r\^

xt,

).

Y"

XYb EX

Y xy &Yv

)oA 4 v& Xpxu

Y Xyo roYv
Y tD

xT

YvY,

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,l i;

rrt

(f

n .:t

-.<-

3{ tt-UU GqE frnh ''ecL c{6\6,rgArnChV 5

"t ./13. What is the first three amino acid sequence the'peptideencoded this gene? of by \r (refer to the codontable on the last page) A. Met-Glu-Asp

B'Phe-Lvs-Pro

3:fr1-tl'il,t-si'
,/

E.Notenough information determin{larou5o*ftareafu to rtler,, posSi}t,ruhry -fryrg

14.If the entire coding sequence above(i.e from the ATG to TAA, and excluding intron) is 636 nucleotideslong, how many aminoacidsin the protein that this genewould

f rofrvcprnnl' | rs'r\ .' ,,t rl 0o41 it^i3 0s'h{ , noJpt4

speciff? 4.2t2 B.2ll io$ t, a s,t+ccJn

c.2rc

D.2t3 E. Not enough information determine to

l tz -1

.-A

''<.'=v

I#":j
/to+ r) / (A+ c)
,)

v.Ai G f \

'-t

'1)) Si,;6f1
c. ii,iii,i, i,ii,v
i. : j' :

-r

xiii,w
fi

the first division in meiosis (meiotic division f) or mitosis. ? D.lVleiosisand mitosis both produce cellsthat are geneticallyidentical.ftCovd4qfy-r th ^486 mitosisandmeiosis, parental is Cnploid. cell the fl flnboth ( ,.^ I ^.rt I f . -, J

I Pn k^lY'iJ tr

4 A Whentheir child is born, what is the chanceofhimlher zuffering from the same disease? A. Atleast2i%o B. At least50%

',^

' c. Af{dffitb/o-ii....

D. 100% E. Not enoughinformation to determine

9. What is the type of mutation? A.Insertion B. Deletion C. Transposition D,Inversion E. Translocation

\,t
1

B. It does not causeanv change to the protein. any doesnot cause change thp pgotein. ' -l t the protein.r,, It shortens the proiein.,.i C,It C, shortens J , 'D. It lengthens protein. , the {'. ,g

provided thelastpage, *J,urrpnq*tt .Cb! on codon table 10.Refer thegenetic to gene, of whatis theeffect such[rutatt6r in represents of thecodons the one tg]h" J - J (', p-t"irritencodes? \ J t / /f' A.It replaces amino withanother one acid Wt f \ l,l UAC ! '
\ \ j

n^RND

is E.Noprotein exqY

y}

J'

a".,ti6;\,eJ*#

: DNA r",rowing sequence

two regions 5'.......:AryAiffiCCCGGG..........TTTCCCA']MGGG....f#',represents gene.Dotted lines refer to unknown sequences surroundingthe of a paiicdlar exon in a indicatednucleotides.

l,^.,-fafz,,/rr.*u,ffi" IJfufi""tary sequence? DNA

!

-+

-', J

CCCGGGTTTCAT....3' ..........CCCTTTGGGAAA........... . 8 .5' . .,. . . .. ..AUGtu{{CCCGGG..,. .. . .UWCCCAAAGGG .. ...3' A ... .. ..CCCTTTGGGA'Tq.A. .' ....CCCGGGTTTAA. . ..'.3' c . 5' . . ...3' .. D.5' ..CCCUlruGGGAirAiA.. . . . . ..CCCGGGUUUALAuA . . E . 3 '. . .. .. . . ....TACTTTGGGCCC.. . . ..... . fuMGGGTTTGGG.. . . .5' . ..
4. 5' -rl

12.Which probe shgu!(be chosenfor the Southe4 Blot to identify the above

ff,

3', 3',
T3'

fr*P,u*q

C. 5' CCCTTTGGGAJEA. D. 5' CCCTTTGGGA.A\r{........ E.Any oneof theabove.

AT3'& ,*t ^Affi

A
,7

il

\rvlo, tA^.{a

{, to the only act from the"5,,r"end3'end to synthesize new , \,/r'17. Why do DNA polymerases n, /rucleotide? to \ y A. The triphosphateat the 5' end is unstable provideenergyfor the synthesis. B. Thereis a capat the 5' endto block synthesis. C. The DNA polymerase bind to the OH goxp at the 3' endfirst andthenjoin it will with a new nucleotide molecule. D. A, B, andC arecorrect. E. A, B, andC areincorrect. rc.Different forms of the samegenearecblled A. alleles B. gametes C. pairsof gene D. homologous chromosomes

tJ ..''. , I ,*u"ni"b"#JrIA*JL ," into nucleotides ageneti. t many of . y't.The insertionhow

V mecodethanthe insertionof othernumbers nucleotides? of f

E.gene locus

A. I

8.2
c.4 D.5 r \
f \ /'

ri 'l

fl J ,
J

. \ J

E.6

t -../"

, ",

-\

J"

'il

phosphate of 20. whb f,r}nnr#O that the DNA is composed the sugardeoxyribose, V{ - .! gtoups,M nitrogenous bases? A. P. A. Levene B. FredrickGriffith C. HerbertBoyer D. Erwin Chargaff E. FriedrichMiescher 21. The two pyrimidines in the DNA are ( ). The two purinesare ( A. cytosineand thyminel guanineand adenine B. thymine and guanine;adenineand cytosine C. guanineand adenine;thymine and cytosine D. guanineand cytosine;adenineand thymine

f't ),J)\.\

. ,/ v

that producestwo identical DNA . i'Li.What is the nameof the DNA replicationprocess parentstrandand one daughterstrand? molecules,eachconsistingof one "/ A. Conservative DNA replication B. Semiconservative DNA replication C. Parent-daughter DNA replication D. Dispersivereplication E. None of the aboveis correct

is A/ ,t,Which of the following statements not true? V +( Fredrick Griffith discorvered hereditarymaterialscould be transferredfrom one that bacteriumtoanother.{ $ Hersheyand Chaseusedradioactiveisotopesto label phageprotein and phageDNA and found that DNA was geneticmaterial. I C. There are 46Qk"gggfggp! in human soilatic..nr. r3y4m F D. The condition in-EfficfiT personhasan abnormalnumberof chromosor4es c$led is
z\ltrl Rtffi-w-drolne

peoplehavethreechromosomes 2l{

J, r { t \ I | ,.\' 1 ) )
X*"r: ^l-',

24.Whodiscovered inside and "nuclein" cells f,#isrf,r\"rf;

A.P.A.Levene B.Fr e d rickcriffith C. Thomas Hunt Morgan ri 'l l'. : -\ , )* J*

producing blunt endsare chosenover enzymes B. Restriction enzymes producing sticky endsfor geneticcloning. .A C. Liposomesare lipid basedsystems deliver DNA into cells. { to pf, Antibiotic markersand blue-white selectionare commonlyusedfor the selectionof plasmids. qgrcmbinant B. Virusescan deliver geneticmaterialsandintegratethem into the host's genome. below appliesto the techniqueof DNA sequencing? r,/ 26, Which of the statements 2( lt was inventedby Boyer and Cohen. 5An7+r B. It requires useof reshictionenzymes. the ( It usesoneof the four Sboxy-ribonucleic acidsasa "stopping"reagent. productis madefrom its owR3' to 5' direction. sequencing }('Th" E. It requires the knowledgeof gel electrophoresis.

".:V

\t./

V ,/

Zl. Which of the following correctly describes dideoxy-nucleotide? a A. Two oxygenmolecules missingat the 3' and4' carbonatompositions. are B. Two oxygenmoleculesare nissing at the 2' anil3' carbon atom positions. C. Two oxygenmolecules missingatthe4' and5' carbonatompositionsare D. Two oxygenmolecules missing-- ---- and4' carbonatompositions. are -a atthe2' E. None of the aboveis correct

')cr

v I
I

r/ ) {}
.{

1 ,
/ {

XiY
caused a mutationon both of her X 28. Mr Lee's motherhad a rarerecessive disease by chromosomes his father andhis wife havethe normal genotypes but (they arenot 1' Uf Xa . , caniers). What is the risk of Mr Lee's daughter? y A. Shewill definitely be a carrier but will not be sick B. Shewill get the disease inheritedfrom her grandmother. (about50%chance). C: Shemay be a carrierfor the disease D. Shewill not be a carrier. \\ E. None of the aboveis correct. ?'\

,YX
XrhR F/,r :Y XY YY

\ }i:1r
) '\

'Lj

o
Ct/

B. X D. E.
./

Huntinglon disease neurologicaldisorder Down's syndrorne chromosome disorder Tay Sachsdisease blindness AII the aboveare correctlv,matched

31. Which of these autosomal diseasesis a dominant trait?

A. B. C. D. E.

Cystic fibrosis Iluntington disease Down's syndrome (PKU) Phenylkeytonuria Sicklecell anemia

.:

F t'

d)
f: 3;i''"
C. l:2:l D . l :l E. None of the aboveis correct

.o\"-u1$"1n*
A n" ''s'a n
r ftr"

is phenotlpic ratio . / ,r.what the "r#ffirfi:r:rrr' v

;'t'g;nd

on A o

33. When inheritanceof a particulartrait follows a patternof ( ), individuals that are heterozygous the trait havesomeylgilnldugegphenotypes of the two homozygous for individuali. dominantand recessive A. incompletedominance B. co-dominance C. completedominance D. polygenic inheritance E. Noneof the aboveis correct. \ ., I

1V ,H:'fil1il'J'useturfor 11
B.tr a n sformation C. cloning D. DNA sequencing E. genetransfer. r ' ) ( ,', ?/ \J

J, r i t. t I
\\l

r,.\ 3:
t \J, \ J

Ju

paragraph: rouo*ing auestifr/s\s ryf !t+,od

Diseaset')i tj*udt to be an inheritablegeneticdisorder. A commonmutation in the sequenctdfthe codingstrandfor the geneX from: s'..A\rqA TAT CCCGGGTCT..3' to s'..A\rM TGA CCCGGG TCT..3' (refertothe codontableon the lastprage) {.-r,, {tsy -r'

f \')'

vv

i:':'

r,/ lS. ii thf nameof'mutation? A. Nonsense mutation.Sotr^q B. Silent mutation. C. Point mutation. D. Substitutionmutation E. Noneofthe aboveis correct.

,qs TJ. ?^ tdt&at

6tV Sry-

l0 / tu. Which sequence(s) give you alsilentmu will A. 5'..4A,{ TAT CCC GGG TCG..3' B. s'..A.l44 ccc rAr 99949J.:3-' L\s f)- Pbs C. 5'..Atu{TATCCCGGTTCT..3'Lrx-6*- fa D. 5'..AAG TAT CCCGGTTCG..3' h,t \, Yuo E. AII ofthe above sequences. y.

t,a\< 1t'"an^aof the

G\

UrV J.tr
fer

eene X?

Grq sqQ'Y *r

in lhich of the follgwing aminoacidshasthe mostredundancy codonusage? Startcodon -'*- l' " {. .*.' X Stopcodons 3 C. Leucine (LeuX D. Valine (Val)Y V

',at

(rv02 E. Lysine
t) t''. ,,,/rr.whichofthefollowingisNortrue? .,r , I g. Aminoacids building are blocks a polypeptide4t,rt "'H. of r t \ I 'Bi. Deoxyribonucleotides buitdingbtocks DNA an(ftx$ of are /, J ) together. Y K e*iOL linkage joiningtrvoaminJacids is J a D. Histones present eukaryotic are in chromosffes f \ J 'J *:E. All arecorrect r l \ tr J
o*,uado* geneticcodonsand amino acids? 39. Which statement(s)ris/die,$OT X- one aminoacid cpn\" s5fgn"a by morethanonecodon #more thanonetype of aminoacidsp .4,O"f"4onfcgilsp\ci! rC. )Mr*atiohs aftht *ilr6 baseof the codonhavea moreprofoundeffectthanth

t'

Xa

E. B antl C

it
V '

+O.I havetwo dogs- with greenandwhite hair, respectively. do not know their I genot5pes, do know that greenis the dominantcolor. In order to determinethe exact but geneticmake-up,I crossed them and get 4 offsprings. Which of the following is correct? if ttre green:white ratio is2:2, the greenparntis probably pure-bredand the white a Y hybrid Xq if I get 3 greenand I white, the parentswere probablyboth hybrids q<if I get 3 greenand I white, the greanis probablya pure-bredand the white a hybrid D. if they were all green,both parent dogswere probably pure bred. E. None of the answersis correct. sl el l \^J l'^J aia
y'-l

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La

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41. How manychromosomes therein a humr are

4.22 8.23 c.24 D.44 8.46

+2. First usedin geneticstudies Thomas Morganin the early 1900's,this organism by hasbecomethe standard experimentalorganismfor geneticresemchers.What is this organism? pea A. Garden plant. B. Fruit fly. C. A type ofbacteria. D. Yeast.

,,/ ' n'

E' Mouse' , .t'43.During Southern analysis, can in"r"ur"ffi the blot how one

t) tt'

"rl{h d["-t (', ofthedetectionprocess? \ J rr' A.Bydecreasingthetemperature.Ivt.lreadwl f \ ' ) ) B. By addingmore probeand loadirg rponfDX* il4oihy'gef C. By reducing saltconcentration ttretryUriAllatffi buffer.indc6'4 , , / the lrl D. Bi usingra-dioactive probesratheltha[non-ffioactive probes. frna k0(tion E. By increasinethe the probe to the targCt. \Sp{hornobgi'of
r/ r \ [\--l Siwllau+fi4 'f 'p-aL!.

44. ttd \r,t6rt frot *. ror meiosis? -.2 A. It occurg)oriltrn the testis andovary.f
B. C. D. E. It is'irrfolvedin producinghaploidceihT It givesrise to identical cellsto the parent cells. nal{og It is involvedin producing spennandeggcells. All of the abovearetrue
.: ::l r:i-\/

rtrl
\

t'A

is a I /a5, Which of the following steps NOT usedto Feggerce genome? suchas 5-10Kilo bases. >4( DigestganomicDNA into smallerfragments V" $ Clone small DNA fragmentsinto plasmids. C. funplifi/ DNA templatesfor sequencing reactions. p( ferform Southernblots to constructmap. E. All of the aboveare used.

t2
V 46- IrDNA, adenine alwayspairswith ( ). alwayspairswith ( ), while guanine A. guanine,thymine B. thymine, cytosine C. cytosine,adenine D. guanine,adenine E. thymine,adenine

tlU.1 I '*

^n / 47, If four nucleotidesspeciff onecodon,how manypossiblereadingframescanoneget

from a doublestranded DNA molecule?

,
,'.:(,

*.i
C .6

lovc &&hs+r'^' ' a')f +{ta^*A

tt,-'),,b

,}o

D. 8
E. l0

*xL

vA.

,/+9. Watsonand Crick are famousfor discovering

the structure of DNA. B. the chemicalcomponents DNA. of ?, C. the basepairing of DNA. D. that DNA replicationis semiconryrlative. .. E. thatDNA is the geneticmaterial. ,
)

j ?'f

]'\

rtg. A.
B. C. D. E.

rJ
oc$lf

nucleicacidsare

J' :'i

fl

(Met) (referto the codontableon the lastpage)? 50. What is the anticodonof methionine I r A. 3'AUG 5' 3 \Ahc 5 B. 3'ATG 5' C. 3'UA C 5' D. 3'TAC 5' E. Not enoughinformation to determine.

5 ' A\A?T

l3

Snd,base in *odun

til
c
E tt

C. A
Ser $g,r 5,g.r 5cr

ffi
,,,Glfis,,
. gT0tr f.fO ."

L3 G

Ffte Phe L+u La*r

8.su f.eu -cu Lsu
*ls' *le *l*

TEr, , Tfr,
*Jls *li_s,
8|fi

',u,,. E.
,,i[ G

STOft

:EL
:R'

ri*

sTntr

Fro
'Fro

&.,19

(D ,tft, ftf. {*

ffi

il*st r/.u$

Fro Fru T:ftr Thr T*rr

SI

,ftrs
5ss Ssr

Sl;n

frts Ats

U G A
F,
U ffi

tr ar
s

s
*'il

s
3

\;*'

.ren L'.Ys *-ys

fisF
A$p G$.* 6$u.,

Gt

*ru

T,&rr n

&rs
trgrllf (ilv cfy G'tr

lt

tlla$ Va* Uat

Afa *la Ala

U G

{i'it\'J

tl,i", :,

.{- ..

\' '

cr

I (' l f
i ... r.!l

Se+ -

pennf@Wwf?
u/ *:, Ff-Biondsca*beft.nned: / (a) duri*gDNA rep,EeationJ <: l'
,:,ri.l! "

DNA *1 ry-n**n and,RNA,Zrf * A and C

{d} (e) A,B and C

Bf,within an RNA molecule I lfttF

2.
,(a)

,1I
or their mRNA?

1{b)

(e) (d) (e) Ban d C

.f{t')

3. Which of he following RNA moleculesserve as a "scaffold" or support within the translational machinery? (a) Ribosomal RNA (b) Transfer RNA (c) Messenger RNA (d) Srnall nuclear RNA (e) None of the above

(y

4. (a) (b) (c) (d) (e)

Theprocess of transcription is required to produce: DNA only mRNA only mRNA and rRNA only mRNA and IRNA only mRNA, rRNA and IRNA

{*y
@u

5- lMrich of the following is not a form of gene mutation? (a) Inversion )r,-

ft) (c) (d) (e)

6. (a) (b) (c) (d) (e)

Insertion# r Deletion3'., . DuplicatiohJ-' Replication.'

The process of homologous recombination during the meiosis is also best known as: exchange translocation cross-over transposition none of the above

,.y

\ri 7. Geneallele W encodesa non-functional protein. What is the most likely mode,of genetic inheritance for the diseasethat resultsonly from the presenceof two copies of the allele W and transmitted bv human chromosomernlrnber22?

(a) ft) (c) (d) (e)

Autosomal, recessive Autosomal, dominant Sex-linked, red,(sive Sex-linked, dofrirant Not enough inforination whetherit is dominant or recessive. on

fln

r ::: 'l:'La1 .E l' t:

t-

.,

/*.

(a) Singlepoint rnutationin the middle of the intron for the gene\4Q/ ' , , (b) krsertionof,threenucleotidesin the fust exonof the g|(" SntCfiVm\) (.9)Deletion of onenucleotide in the fiffion of the genq,a (d) A and C
Not enough infortnatisn to ptedict it

From the Q,uestion7, what is thd least likely reason that could geeountfor the loss of protein fimetioneneodedbythealfeleVrt? / NUtUaNu{'O

a a ?-

{x{
l:-

t I I
t

t
t

sequnse 5'--.AA
is the first tlnee amino'aeid sequenceof

(a) Met-Glu-Asp

I .
I

ffrpNn

8i Xtf:l*

s'ft$u 3' ccc{6tc Iooclvvu@

r{+-{.,v<r}c,q-l'

_:

C>
for
n\onl,ht)

/(dFrhich of thefollowing r6/lt is double-stranded wi V,r,tU^c1i pollmer iS.).,* is a backbone.

prope,rties is/are corrmt?

-Zr{1ip.rn1bt linked.fromone nucleotideto the nextbfpf;osphare

r\--

rffi;

I vl

(c) The pol5rrner carries a net negative charge .7 (d) A and B (e) A and C

N, c
A-andmRNAXpr un"r*r,;t4r'"{X;14^.gtJpk 4s ',, ' 'rn,.lrtftrlti"lM, (,
\/

/ ItDrring the synthesisofplgte[gs i W (a) the decodingprocessisE-ediatetl

(b) proteins can be heavily modified by attachment of,sugar (c) IRNA can be recycled aud re-charged with am-inoacidsf (d) A and B

(e ) B a n d C

12. The processof exchange between two\-+ different parts of non-hornologous chromcsoms is ---/ bestlsrownas: (a) exchange (b) translocation (c) cross-over (d) kansposition I l r tt (e) recombination i Y''\,' Question 13 to 15 refer to the following paragraph: 'r
\ ', .-'

The following figurerepresents restrictionmap for a geneS ry{blbgj{qindicated byEt ,82 the and E3, generating fragment A, B and C. A specifi 3 corresponding a region that covers to part ofthe fraghent C (Indic?tetlylhqlhick line) was used to carry out hybridisationafter Southem blot. Disease resultsfrom a mutatiolin BOTH allelesof S
the gene at E2 site that have destroyed this recognisation site.

I lt

i
!:-

y/fl.WWeh of the following$4ternent(s) is/areTRtlE for anafysisoftliewildtlpegrtleleS? *) Fragment C travels theflrstcs&duling gel b) FragryentA andB represent*llelesof the (e) Emyrnes'that recognise El and E2 arc called DNA (d) Theprobe m-ust homotrogous possibleto the region of interest be as (e) Noneof the:above 14- Dwing ur*eprdbing, how can one inercase the specifieity.and aeeuraelr,of$edetesttoh? reducing the temperattxe, !*l !y (b) BV increasing the temperatu$ the probe u{td,h" target iri gy increasin[the degreeof irornology,between (d) A and C

a_@gr /(t ther@egg, how many fragmentswill berevealed on the blot after DNA n"strictionI t/ i "f enzlqresthat cut Fl,Ez andE3 sites, and then followed by Southemblotting \7 fiatffiG
using ttre probeindicated abo*e? (a) I

O r t\S-ifn" rnapaboverepresents mapof thewildtlpe allele S. For an individualbeing s the

(e) tsandC

; V> .i'i :

oho ler',4tl^ . . tu k9'

,? oW lq^af{t^
c

t./

(d) 4 (e) Unknown

(a) (b) (c) (d) (e) Not enough infbrmation to determine

If alsiri RrySA* cytosine compositionof ll%o,what is its compositionof its aderlifrEs? t^:t i+s -siugtekd t5% ' Slre<,,r.ltgcf GC ' 20% 25% (4) e 35%

17. Which of the bllowing organisms/cellshasits RNA as the source of geneticmaterial? (a) Certain types of bacteria ft) All human cells

i"i *lVi*""'

(d) Certain mice cells (e) None of the above fol.lowing staternentscorrectly describes the relative order of organization?(< C 18- Whieh of thesmaller than) sign denotes (a) (b) (c) (d) (e) Tissuer< Cell < Chrornosome( Nucleus < DNA Nucleotide < DNA < Nucleus < Chromosome< < Tissue< Nucleus'< Cell < Chromosome DNA Cell < Tissue < Nucleus < Chromosome< DN{A None of the aboveorder is conect ide

5risq$
leotide ucleotide

t'L'c\{ c{ i "l c {' t* 0.,{ c.f,wri,t. r

-:-:-a.:

-t "F 'E 4

(a) 22

1.-9..' F*

rf **y';ytr>* ef efuornosome$ *iere iS t6eski*uelX u$g are

.@$?

[3]3i'-*tt' (d) 46
(e) None
restriction digestsv/

r&1n'ts
J

' ^t\\\

' t$ s rr$*'

M:
e/

20. Theprocess detecting presence onegene interest {f_E[n]eps of DNA the of in of of tuglTts by Southeyn the blottingwouldreqtrire followingstenflj!!9 .. (a)
.v,/' (b) electrophoresis (c) transferandprobing (d) h)&idiz4tisn,,/ (e)'cloning 21. Mitssis canonly takeplace during the processtat generates (a) sperryrc/(s (b) ovum $ls , (c) skin c{ts,,/ (d) A andB (e) B and C

,y'

22. tvfultipletypesof proteinsin our body canbe encoded from onesingle genebecause of:

t " l @/

(b) &<Ffcation of proteinsafter synthesis t/ (c) high degree geneduplications of (d) A andB (e) ts andC
23- Agoodplasrnid used in genetic engineering must contain one ormore of the following

features: \'/ ;;s";freplication 6) marker ''/ 1U;*tiUiotic-resistantgeneas a selection ,/ (c) multiplepotentialsitesfor the insertto be ligatedinto ,r/ (d) A andB

the bacteria that survive &e antibiotics selection still fail to give the intended

rnsert,This most likely because plasmids: is the / {a) keepsduplicatingin the host (b) undergoextensiveinsertion in the hsst
(c) self-ligate before they are transfened to the host (d) fail to be tansferred to the host (") no longer proceed with DNA replication "* . r f-"\ L t,/ - /. ' l: -

25. Which of the following enz).mes is/are NOT necessaf,)r making a recombinant plasmid for in the test tube? (a) Reskiction endonucleasesr" (b).ligases J (c) DNApolymerase (d) A andB (e) B andC

l$(a) \." (b) (c) (d) (e)

fhs,:gfatS is,sue that is currentXyfaeed by tlte genetie gnglneering industr-y is: the intellectual poperties rights the social, ethicat and biosafety issues the high demand of manpower \1 the intense conepelition frorn othenindustries the prospectsdwing the economic down-turn

t-

^.'/',
27. Missing.28. Evolutionof a species could be drivenby: (a) adaptationof the sppciesto new environment w (b) gene mutation (c) genetic engineering "/ (d) A and B {e) A, B and C V/.

e)

29. Vlhich of the,fonowing statement(s) is/are TRUE? (a) Genorne of a{viruq allows reading of multiple frames from a sequence

(b) Large proport'on:of thehumangl**".obes fo.r-BroteSs F | (c) Bacterial DNA is nackased hw histone nrotd.indf ; \ (c) BacierialDNA is packaged histone protdin{by f (d) A and B
(e) B and C 30- All living organisms contain: (a) DNA as the genetic material (b) RNA as the genetic material (c) DNA,_rd RNA as the genetic material (d) DN,A.or RNA as the geneticmaterial (e) proteins-asthe genetic material

vr

. -sE. k

9"eu T)
.

E lj t:

t:
t:

{ ,
J

3' I fuage9), the strandencodedgeneA inthe following sequence .{lnfigure G.ATCCA.-.-GGTGCA 5' is ealled 6gc\tvq ! a.,ue i''-aS|ne (I) ternplatestran{ (2) coding strandJ .-h,r;.rgta-{ecv$" tetnte atth*e (3) activestrand{ (4) inactive strand.{ (5) sensestrandJ A. | &.2 i B. l&3

I t 2

'p

{ *

I {
l: i *"
li. 4' 1. i
!

c.2 &3 D.2 &.4 E. l &s

t t:

Figure I and Table I (pages 9 & l0), the first amino acid of the pol5peptide ff.Yasdon "is A. Cysteine(Cys) B. Threonine(Thr) C: Isoleucine(Ile) D- Aspartic acid (Asp) E. Leucine (Leu) 1 (page9), the anti-senseRNA sequencefor gene A is '7{l^figure x- 5'ACGTGG.-...-ACGTAG3', 5' B. 3 ' ACGUGG......A CGUA G C. 3' UGCACC......UGCAUC 5' . 3' D- 5' UGCACC......UGCAUC 3' ,E- 5',ACG-UGG......ACGUAG f-, lU 4.'Cells get theiinames from a scientist called A. Robert Hooke B. GreEorMaudel ' '/\( C. JamesWhtrddn

t i

:
I
I

? t

D. Francis Cftt
E. StanlevCohen F. Alicia Xiao (opps am I the one???)

T
a

-?---->), of 5. How manrr(diff6repilkinds chromosomecan be found in men?

A.24 B. 23 c. 25 D. 22

@.

n;1

6. Which statementis tq,refor mitosis? --

A..It produ"u" effi;

B. C. D. E,

et-----

It produceshapioidcells S | fi The daughtercells are identi'calto the cell that gives rise to them I It produces sp6rmsor eggs f No of the above
I

" B. The nalne means"true cell" -\ -. C. The nucleus contains some 2 melers in length ofENA -f D. It has a circular bit of DNA called plasmid that encode for antibisticdesboying I enzymes E, Afterthe fianscription, nRNA will be spliced to rernove introns f .{:V,tttictris not the method for genetransfer to animal cells? A. Microinjectisn ,./ B. Adenovirus

7. Which staternnt ir:g!!gC fgr eukaryote? A. It hasintronsandexons \ '

- onlYts?t$t't] : {.oJ""[ny*,species D. Electroporation'

E. Liposome.7
ic ich statementis nq!_.lkqgjorthe requirement of genetic material? It rnust be able to- replicatel

I
\-7

D\$F l[Nl!

It must be able to control exprssionof tmits -T' It must be able to introducg mutations of that the organism can adapt ts the new environment D. It must be able to encode the seqtrence of,proteins T infro\J Noqe g{the above .

L
B. C. D. E.

!+*utl

?\

rmatic cells taken from wornan alwaVs ;paiiolx chromosorn"F

a pair of Y chrornosomes XY XXY Nsne of the above

11.Osw,aldAvery,Cglin Macleod,&' elyn'McC#-at'ssddthe following experimentto {' \ prove that DNA might be the geneticmaterial A. DNA from heat-killed virulent bacteria could passgeneticcode to avirulentbacteria B. Radiolabelliiigphosphorous(DN,A.)could passon to a new geneftion ot@89 {s6v,ry-rhc{t C. The percentage content of eachof the f,ournuclestideswas)he sirme(.."'ft| ' D. DNA was labeledwith heavy isotope ofnitrogen to prove that DNA was replicated

in a semi-conservative manner

gtoh I Mg(<[Crrrn"--..'.*.-"_""

4!

'A'KasL\t'{\
,.RgSaNt'-E- X-ray.crystallographic datawas usedto prove that DNA was in a doublehelical /-\ struefure -\ tectrnique not usefor DNA sequencing? is ,fu.lgnicn '(t ,{ Gene cloning l( \_,/*

B. PCR X,/

C. Dideolyinalog qf nucleotidq/ v y' D- Gel electrb-p-hb-resis E. None of the above 13. Celera Genomicsis led by n A- CraigVenter B- JamesWatson C- Francis Collins D. StanleyCohen E. French Anderson

f)"
Anti-sensetechno)Qgy /

?-&
dyeand sorting them by

t {Wnatis not tme on Bt-corn? / X. It containsa genefrom Bacillus thuringi.nsis 1 v B. The Bt gene is toxic to the Europeancom borer f .t C- The Bt corn may producepollen that could kill caterpillarsof.themonarch butterfl \/ D- Theyhave been grown commercially./
E- None of the above

v-r

1 6 ,which is the first cloned animal A. Dolly B. Tracy C. Herman D. Mickey Polly

ich statementis not true on the mutant Ntfethuselah A mutant fruit fly that lives more than f dbd"Vs, a6o* one-third longerthan the rest A key to discoverthe first so calledclock gene C- The work is in progressin the laboratoryof Seymour Benzer of the California \ Institute of Technology D- It is usedto study growth genesin fish, pigs,'sheepand cows E. None of the above

ouol &tt Ktor'r$ nn,n

t v'NJ-i: { hv [t:l Tr'*lns i ntr."{r"
r-)

* n. rtreyirapaat"ii**1-;*;,;"f;r"
E. They "ne

18- whieh state,nremtt n6t Euq on restrietion enzyrnes? i A. Speeialproteins that cut DNA -T B. They eanjoir* iwo DIrIA fr*gments together ' . C. They generatestieky and blunt ends"f

sprcifi*, and able to recognizespecific nNa squcnces

against ftireign DAIA T

lC. Which statementis not true for telomers o I A. they are the tiny ties at the end6Feachchromosomes -f B- they shorten eae,h time the eells replicate -l' C. they can prograrn cell death {

L
I
ot \

3j Hffi":il::,Tu

thetelomers lengthen life span cellsin cultur" and the of l*

lF , Dr B

"<lQ

is not true on why pig is chosenfor xenotransplantation ){Vni"Astatement A. They have natural htrman-like proteins so no rejection will ooour after transplantation B. They are dornestieated,clearr and easyto breed T. i C. They are the size of human .f D. They are similar to human suchas anatomicaland physiological aspect -1 E. None of the above '

n /1

)sh

statementis not true for cystic fibrosis (CF) 'l 7/*Anh ' A. Patientshave excessiveproduction of abnormally thick and,stiekyrnucus T B, Dig e stive enz)ilnsofCFpatien t s willd e s t ro y t h e irop a n c re a s ' wn : C- Preventstlre movernent of chloride ions out of the cells f D. Retrovirus is the methodsof choiceto deliver the CF gend f [iyosaw E- Drugs such as pulmozyme and ibuprofen can reducelung deteriolation and infection 2L.Bo*emarrow colls are called' A. germ-line cells B- somaticcells C- stem',cells D. embryoniccells E. embryonicstem cells 23. Which statement not ftue on variableNumber Tandem Repeats'(VNTRs) is

-4

,t..'

\r

[t\',5{n[{ B.

A- They are in introns and supply no relevant geneticinformation T Paternitytesting basedon VNTRs canbe donewhen one or both of the parentsare unavailable -i C . Each sequencecan contain Jnywherefrom twenty to one hundredbase pairs -( D . They are used to makepptes ior}$Xand RAPD for DNA fingerprintt"* E. No twp pggp'le,unlessthey,are-identicatr,hvins, [aye.the samepatterns of VNTR

24- which of ttre following protein is not producedin geneticall,ymodified microorganisms? / A. Hurnan Insulin / t B- Human growth hormone.,,/ / Q- Bovine s=omatotroptrin CuW @. u l-antitrypsin -fyataft u E- calf chymosin./

25. Anange the proceduresof cloning human insulin into baeteria: 1) Restriction enzyme {igestion I ' ) Transformation 'b ' ': , 3) Ligation J'. 'i 4) Clones.selectionusing antibiotics Lf' 5) Protein expression f"' A. 1,2,3,4,5 B. 2,3,1,4,5 c- 3,1,2,4,5 D. 1,3,2,4,5 E. 1,4,3,2,5
t:

D
v
rl

-:(i-

26. Which statementis true on Type II diabetes? A- The diabetes canbe treatedbyh-e injection of insulin F' B- Mostly develops in people bq|ogethe personis 15 years old 1:p C- Ten percent of diabetescasesare\of this type f ,{ D. The diabetes maybe due to overweight E. None of the above
fi

C. Itcausesmastitisin cow J D. Somemilk farmerswill Ueiut out of business / E. All of the above

tr

,/ zs. is Roundup-readytransgenicplants? what

,..'{\ A. Herbicide-resistantcrops Pest-resistant crops The use of antisense RNA technologyi crops Glyphosate-resistant

'r.\ )! ,

29. What isthe target gene of,Faw Saw Tomato? A. Polygalacturonase B. Ethyleneproducing gene C. Flavordevelopinggene D. Color developi.ng gene E. Noneof the above

30. the codingsef}uence of,the following gene 5' nccdcn rrA ACA TGC cGT 3, is changed 5' AC@A to TTA ACA TGC CGT 3'- The mutationis called A- Substitution : B. N;;;-\ I

c. Deretion t0YlGff' \SGlv\r" D.rnsertion I

E. Pointmutationl /

klF
/

31. the coding sequence thejlollowing gene 5' ACG GCA TTA ACA TGC CGT 3' iS of changcd to_5'ACG GCAFIITTA ACA TGC CGT 3'. Thd mutationis.called A- Substitution B- Nonsense C. Deletion D- lnsertion

'''\

^dd

A tC/ D. E.

it is suitablefortesfing anti-HIV drugs It is transgenicanimals as a rnodel for hurnan diseases It is usedin the developmentofnew &erapies for cystic fibrosis It is createdby transplantingembryonic human irrirnune cells into a SCID mouse Whichfechnique is not following the principle of hybridization?

PCF
Southernhybridizatioq,/ RFLP

RAPD
Restrictiondigest r { \X -/What is the function of a vectorin genetic engineering? A. Cut DNA intomany fragments B. Carry DNA into a new cell C. Link togethernewlyjoined fragmentsof, DNA D. Maintainand makecopiesof a specific segment DNA of E- None of the above

,b'

,--TBased on the VNTRpatterns in Figure2 (page 10) Daughter-2 (t2) is A- Ml- andMrs. A's biological daughter B. Mrs. A's biological daughter C. Mr. A's biological daughter D, Mr. andMrs. .{'s adopted daughter E. None of the above 36. Which. enzymeproceedsalong a srrdglfande_d molecule of DltlA in order to make thec@? 4. DNA polymerase . B. RNA polymerase C. Helicase D- Topoisomerase E. Primase

37. Which enzl.me is responsiblefor initiation of the unwinding of the supercoiledDNA? -...-, A. DNA polymerase B. RNA polymerase C. Helicase D. Topoisomerase E- Primase can be useful in bioremediationbecause -SSilv1icrobesbreak down organic compounds A. they can ^ _l B. they canbreak down plants Cr' I C- they canbreak down animals D. they canbreak down nutrients E- noneof the above 39. Which is not one of the stepsin (polymerasechain reaction) PCR? A- Denaturation a B. Ligation2q C- Replication D. Cyclesrepeats

/ ^2::l::
2{t

ts

Which statementis not true for the useof gene therapyin our presenttime? A. A patientwith ADA-SCID was the first case being treated-f that are affeCted a single-gene B. Genetherapywas usedto treat genetic diseases by defect f C . So far the'technology cannot replacedefective genewith normal gene D. Somaticand germ-line cells were usedfor genetherapy f E. Liposome is used for the gene transfet 1

.0'

it -t 1 1 t ,r '

Set B

1. Ilm trjnng to identify a natural (not geneticallymodiflied)cell, so use.amicroscope

and see:

A.-"";"l"us-:so the cell rnustbet prokffi B. a virus: sothe cell must be g(okaryote C. a virus - sothe cell must be irr{aryote D. a plasmid - so the cell rnustbe eukafftic E. None of theabove is correct

vimsf' ryrftcirrl15. r=---J-)

lry

l:ntcfu{ttt

' 2- Selective is: breeding A. basedon choosing individual genesthat encodefor favorable phenotypes B. basedon choosing=plenglpgcthat may be encodedby particular genes C. more efficient thaa genetieengineeringto improve stocks D- no longer utilized as geneticengineeringhastaken over tA) F E. None Jrtlt above is"correct 3. The DNA doublehelix, as describedUy FG-o56 A. DNA wound around protein B. DNA and RNA wound aroundprotein C, One strandof,DNA D. Two strandsof DNA E. None of theabove is correct i. ' d:cflis made up of:

the DNA doublehelix: ^"discoveryof A. enablescientiststo understandthe natureof the geneticmaterial "

A.

and amino acids

B. $n,,pgrurr-E u-r

r, tt .tiaEtc_rl

cr-al/ ,q{tRj

not hrur4','1i

and a template rase,hucleotides template rnpla Dl Threekjnds,o E.'{fr.;Rinds of RNA, DNA and a template

: 3l?#*lH:?ffi ?: ftsf#${ffi$#
-flltfl r rriFi.tft rFrr$ .ie.raplafu , W

Mcf ir,*Pi *el tt'trc- 3 kiv.ta tl RNfi'

I fr

,1 ,

1- You want to replicate a piece of DNA in order to have a large quantity for analysis. What sho-uld the ioorr. of your actions? be A- Transforr* bacterial cells, select clo:resby bluelwhite and antibiotic rcsistance., ligate into a vector, extraotthe plasmidDNA from the bacteria B. Transfurm bacterialcells,selectclone by blue/whiteand antibioticresistance, ligate into a vector,extractthe genomic DNA from the bacteria C. Ligate the genomicDNA, transform bacterialcells,selectclonesbyblue/white and antibiotic resistance, extract the plasrnid DNA from the baeteria D. Ligate into a plasmid, transform bacterial cells, selectclonesby blue/-white and artibiotic resistance, exhact the plasmid DNA from the bacteria " ,-; E. None of the aboveis conect

I t 8. j I want to PCR amplify a fragrnentof DNA whose sequence do not know: e. I will needto seotrence fragmentfirst in orrlerto nlan nrirner.,/* r.i.'r' I t\ll the I can usepniversal primers for the primers for &e PCR that bind any sequenceof y DNA '/ DNA '/ x C. I can certainly ligate thg the fragment, after cutting them using restriction

enzymes thaigiv;--,-ffi";ky en{glto a plasirid vectorcut,infi" ;";;;;;;;;

--1 --

sequenceon the ve*ctoitE D. I can certainly li

primers for the PCR /

"
then use

E. A1l ofthe abovecould be used

9. Which ofthe following is likely to be occurring in your body at this very moment: A. mitosis B. DNA replication C. Transcription D. Translation r C\ E. Atl of the above

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,y

10.What is correct: qrr\ _ Atar A. mitosis occursonly in $:im cells B. meiosis occursonly inlglrm cells oC. germ cells have double the number of chromosornr6s most other cells rn your than / body D. mitosis would produce8 of 1n (haploid) daughtercells from 2 parentcells E. mitosis would produce8 bf 2n (diploid) daughtercells from 2 parentcells ,'\

'uPtlt-'

I l- tr'm trying to clone a piece DNA into a vector for transfor,rnation into.baoteria: A. If I useblue-white selectiono-nly- all of thewhite colonieswill definitely calry, the DNA of interest \,/ B. If I useantibiotic setegtio:n only, all of the whiteicbldnieswill definitely carry the X DNA of interest. C. I needto useboth antibioticand bluewhitef""rion to ensurethat all of the white colonieswill definitely carry the DNA ofii/terest. D. If I do not use *1". method of seleotion, colonies carrying the DNA of the interest will beSiqe E. None of the above is correct C_, 12.Sequencrng afragr4ent of DNA is basedon a reaction rnostsirnilar to: A. DNA replication B- Translation , ' C. Splicing D. Cloning E. Ligation

/-a. ' absolutelyrequires: ,/ ( 13.)DNAsequencing use of a sinele-seouenced / \-'A. onrnel-/ B. use of a marker that will label newly createdstrandsof DNA C- use of di-deoxi-nucleotides, other special dNTPs thatwill stop chain elongatio, / or D, All of theabove E. None of the above

tA)t

14.Boyer'andCoherrl,, , \- -, A. were the,piOnC'Ers:of geneticengineering N [2.'c\]''r'"r B. createdthe first transgenic otg*irtn C. createdthe first recombinant DNA. which they could then replicate in bacteria D. All of the above answersare correct E. None of the answers is correct ONA 15 .ln transcription: -,. "read" in its 5' to 3' direction thepolymerasq A r Fmplate strgE{d is by B. the template sfiand is "read" in its 3' to 5'direction thekrolffier;q by C. Both shands "read" in their 5' to 3' direction thepolfrEEie are by tD. Both shands !'read" in their 3' to 5' direction thepolymerase are by E. ETof the above could ocour

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.

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I 6. Splicing of the rnRNA A. oecursonly in prokaryotes B. occuFs only in eukaryotes u/ C. occursin viruses \ D. oecursinthe cytoptrasmCIflm ) E. occursonly if the genomeis partieularlylarge

c9/

17.I',m running a gel electrophoresis determinethe sizesof someDNA fragmcnts I've to cut, and one of rny fragmentshas run a little further down the gel than a known standardof 200 bp in length: A. This unknown fragment appearsto be just over 200bp B. This unknown fragment appearsto be just under 200bp qo C. Speedof traveling through the gel and size of the DNA do cor,relate, my thinking is incorrect D- If the bands do n6t occur in exactly the same I cannot assumeanything about the size of the unknown fragment pE. None of the above is correct \,-/ '{ts)Mary had diseasethat is causedby a mutation in one of her genesthat is sex linked \ and recessive.Which of the following is correct? .rfi\ I A. Her son will defrnitely get the disease Her,soq A l I B. Her daughterwill definitely get the disease C. Ifher husbandis neither a carrier nor does he havetho disease, their daughterwill definitely not be a carrier D. Irregardlessof her husband,any child of hers hasa 507ochanceto get the disease E. None of the above is correct li 19 .Recombination of chromosomes : A. Only occurs as a result of a mutation B. Occursonly during rnitosis C. Occursonly during,meiosis D. Ocoursin both mitosis and meiosis E. Occursin most cells, exceptgerm cells
\r.

X O/-X ^
/ -

te) ft

24.The ribosome:
A. B. C. D. ETakesa part in the processof replica)6/ Takesa part in the process transgriffon of Takesa part in the process spliflng of Takes apartin the processof tranilation None of the above is correct

,y1

2 I . D N A fingerprinting is based, the.fact that: on

' / V A- No two individuals have the same amou B. No two individuals have the sarnenumb

C. In no t"woindivi&ratrswill a sir,rgle produce the same nul$er of eftzyme ,D\IA.fragmentg on * get D. In no two individ'ualswill the sameFCR prirnersamplify the sameDNA Ilagments r r) E- None of the above is correct 22TSouthern blotting requires: r " v/ a- Restriction digest DNA V B. Gel electrophoresis/ C. Ligation of DNA fragmflts D . AandB E- A,B andC

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23. As the detective investigating a crime, you ar interestedto see if theDNA in the hair follicle left at the scenematches any of the three prime suspectsUnknown to yiu, -ofr6of,-the.suspects had a kidney transplant-Which of the following is correct? has A. None of the DNA fingerprintingtests will be valid B. The DNA fingerprinting tests will be valid irregardless his transplant of C. RFLP would be valid, but not VNTR D- VNTR would be valid, but not RFLP E- Noneof the aboveis correct 24. 'Which of the following correctly describesthe difference(s)betweenDNA A. The basesare alfldifferent B. ThePhosphat6s all diffgrent are C. Oneof the basesis di{e/t Th4niae g UGA^I D. Oneof the phosphatesYs different E. Noneof the aboveis correct

25. Antibiotic resistance genes: A. arecontained only in eukaryotes B- arecontainedonly in viruses C. areoften used to selectfor clones that have taken up a particular short DNA fragmentof parlicular interest D. areoften used to selectfor clonesthat havetakenup a particularplasmid vector containingthat gene E- noneof the aboveis correct

J.

26. Aplasmid is: i . , A. alinearpiece of DNA foundinaturally all eukary{fc cells in B. a linear piece of DNA fotrndnaturally in alt prokarylgic cells C- a circular piece of DNA found naturally irr somebut not all eukaryotic cells D. a circular piece of DNA foundnaturally in somebut not all prokqryoticcells 4 v E. noneof the aboveis correct 27. Which of the following is true regardingtranslatjon? A. in eukaryotes it occurs in the cytoirlasm ./I & bth eukandotrtpr.ogcqol* B- in prokaryotes in occurs in the cytoplasm /1 -y'$a3,6me-l [,"y1r,.t C- in eukaryotes and prokaryotesit occurs in close associationwith the ribosomeT i l. D- all of the above are correct . E.- noneof the atroveis correct mitosis in a single organism, The result will be: / 28.Two cells simultanerSuslyundergo ' A. 4 daughter cells, each with a different geriome B- 4 daughter cells, with a gnome identical to either that of the first cell or that of the second cell genome oJl a*gourJ ct$s r _ C. 4 daughter'cells; eaehwith the same g daughter cells, each with the samegenome 'o .B r . 16 daughter cells, eachwith a different genome ' C 29.lf, during transcription, a singlenucleotideis mistakenly copiedto a different nucleotide: A. the protein product will necessarily entirely different be B- theprotein product could be entirely different, or could be no different at all C.. nothing will happenbecause protein will be altered only if the mistakesare the rnadeduring translation D. the genotype will change,but the phenotypewill remain unaltered f-i,: ) ' E. noneof the aboveis correct \'-

i1

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30. Which of the following statements correct: is A. chromosomes make up the genome { B. chromosomes containedin everycell in the body exceptred blood cell I are * I C. chromosomes madeup of DNA and protein { are D. chromosomes folmd'inpairs in almostevery cell in thebody { are I' E. all of the above are correct t t 'kY {.J: ;.1

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(5' 1. What is the DNA sequence to 3') determined the following gel (Fig. r)? The in -s DNA sampleswere appliedat the top of the gel.

A. B. C. D. E.

TAACGTCACCTG CTCGACTCCAAT TTTCCCGGCAAA AAAGGGCCCTTT

None of the above

!tii \ ( * I ^^

TOAA
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Fig.1

2. For Fig. l, T, G, C and A represent four reactions, which of the following statements is TRUE? Af l reactionscontainthe sarne template,the sameenzymq the sameprimer,4 deoxyribonucleotides (dNTP) and 4 dideoxyribonucleotides (ddNTP). F All reactionscontainthe sametemplate,the samdenzyme, dif&renlpriryers, + deoxyribonucleotides (dNTP) and 4 dideoxyribonuc Ieotides(ddNT P). fAll reactionscontainthe sametemplate,the sameenzyme, sameprimer,4 the q|$q four dideoxyribonucleotides deoxyribonucleotides (dNTP)and.! (ddNTP). When a ddNTP is incorporatedinto a chainof DNA, the chain cannotbe extended because absence af4OU groupprevents the of formation of a phosphodiester bond with a newnucleodde.F When a ddNTP is incorporatedinto a chainof DNA, the chain cannotbe extended because absence a 5'-phosphate the grouppreventsformation of a of phosphodiester bond with a new nucleotide.p -

(-

D E.

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3. Use the codon table (given asTable I in the A (s) fo Itqlying- s-e.quenee beIow can represent tlqfs ense sequencegly-ala-leu., tgrReptide

t: tr t_

ix\and det$nine"whieh the of

of th$,DNA thatEeclfiqti fhe rP

X.

x ::- egfseqET c. s' )dabcapra 1. -:'

D. A. B and C are all correct

3' s' - GGTpCIFTAF /-

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A' B andc areall incorrect

16-1iL a*le>* f +-^fl"-if f

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Which is NOT true aboutthe geneticcode? [hint: you canrefer to Table l] of The first 2 nucleotides a codon are oft6n'enoughto specify a given amino acid. / codonsdo not specif an amino acid.f 9f"( Some -T \/ Nearly all organismsusethe sarriegeneticcode. There are six different kinds of codonsfor leucine and serinT l @ 4 JY l

A. B. C. D. E.

Some "#8tJlriJ*""u*.MK='"

h-

5. After ten cyclesof PCR, by approximately U@afaglor will the number of DNA Assumemaximum efficiencymoleculesin a samplebe increased? ::
ti

A.

B. C. D. E.

lo 1 0 0 f, 1,000 l0,0oo 100,000

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would requirebase-pairing? 6. Which of the following processes

t

A. B. C. D. E.

Colony hybridization '/ Translation/' / DNA sequencing.u/ 1 usingRFLP J DNA fingerprinting All of the aboverequirebase-pairing.

fr \

for 7- Fig. 2 depictsthecloning of a foreign geneto a given plasmidand,sefection the giving bftre oolor in the gene encodedfor an enzy-,me correct elones- The p-galactosidase presence a substrate. Which arethe phenotypesthat you will usefor selectingthe of clonesof interest?

A. B. C. D. E.

blue colonies Amp resistance, white colonies/ Amp resistance, blue colonies Amp sensitive, white colonies Amp sensitive, None of the aboveis correct

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8. A single mutationat the l00thcodon of GeneA was found.The total proteincontains to 200 amino acids. Whatwill happen protein A produced?
A.

B. C. D. E.

mutation.l protein will be produced if it is a nonsense A shortened eompletelydifferentproteinwill be producedif it is a silenddmutationA A slightly different proteinwill be producedif it is an insertionry'gtation. No proteinwill be pro{ied. None of the aboveis coirect.

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}-\ , 9. Which of the followingoptions is/area good nnatcli?

A. B. C.
D. E-

Mieseh Friedrich ivd ThomasFtrunt

ifihromo-sornes in'specifieo1de1 arranged

nuclein f

production of one GeorgeBeadle and Edward Tatum - one geneoontrols the! enzyme cornein pair and the Walter Sutton and TheodoreBoveri - Chromosomes of chromosomes egg and spermpnite at conception of the above are correct *f"hAl[

f A)

his that disease nelther- fathernor mother with boy is diagnosed a genetic 10. A young &sedeGilftlbe: uncle(mother'sbrother)is also-sic-EThe luflers from,but his rnaternal ,A ( g.) W X E' sex-linked disease.{ adominant, . sex-linked disease. a recessive, '/' arecessive,autosomaldisease. V autosomal disease.. a dominan! It couldbe b or c' XA X^ I w-'i' . (O Y , A_ Ac" 'k ft Aa_ fr= \ frcr {r' c^a*

I l. [note: A. B. C. D. E.

is nucleic acid,which of the following statements TRUE? equalnumber] A:T, C:G 4'G : f+C A:U, C:G A, Statements B and C abovearealltrue A. None of the statements B and C above is true

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A. B. C.

12. You were askedto clone GeneB to a plasmid at a multiple cloningsite. Thecoding iegion of geneB is represented an arrow*(Ihe startcodon is at the left end andthe stop by map is givenin Fig. 3. The multiplecloning codon is at the right end)and a restriction riElus itig.Z. WEiitr site of the giv".,pdffi-td hus allth@.g you use for the most efficient cloning? restriction enzvrne EcoRl Hind/// Baml/7 -r. .t HaeIII in None of the restrictionenzymes Fig. 4 is suitable.

K. ' E

.N

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-_i.j

@gtal

"{9,
Tagf Aluf Smaf

Fig. 4

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below is NOT true for the Human Cenome Project(HGP)? Whiafr shter'nent HGF was begun in 1990. { was usedto break the humangenomein BAC @acterialartifrcialchromosome) l5OKb eaah. B could be that the humangenome sequence In 2000,FresidentClinton announced patented'. N which spentabout $l per baseof the Deterrnined sequence 3 billion bases and telomeres. t They have finished the genomesequencingexceptcentromeres

'{\

Which statementabout bacteria is NOT true?

{.*-

A. B. C. D. E.

Bacteria lack a nucleus.{ Bacteriachromosomeis generallya single copy.T. Bacteria lack the ability of post-translationalmodification. They may not be a eukaryotic proteins. T suitable host to "*prts genomewhen comparing to genomes of eukaryotes. Bacteria have a smaller (use multiple reading frames of the sarneDNA lt ' They overlap plqtein "oJ"r numbersof open readingframethe sequeffitdffinnize by Batteria irurr" op"ronsto regulatemultiple genes using a singlepromoter. T .

[h .x*
B. C. D. E.

-t'td Which statementis NOT true for VNTR (variable nur4bertandemrepeats)?

iAentical.fz t*r'"it'*- irr"*rlic1v"l';"t The VNTR pattern fo, @-Uglgggri$jos'ii by The VNTR pattern cannotbe generated RFLP. f i It is usuallylocatedwithin introns.{ long and bd repeatedin tandem 4 to 40 times. -4 It canbe 20 to 100bases t The VNTR patterncannotbe appliedto bacteria-{ A

n LJ

rd,d0r.fc.{ioknticet

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(l) (2) (3) (4) (5) (6)

(w

Describe th.

"n9j"tu!!!

of

,rA

-:f!f::llOi:US{!l

The geneticmaterialsof the daughtercells are halvedof the parentcell. {' cells are identicalas the parentcell. F of The geneticrnaterials the daughter betweenhomologouschromosomed.Y: Crosiing-over or recornbination:occurs occursbetweenheterologouschromosomes.f Crossing-overor recombination Two daughtercells were produced.J. Four daughtercells were produced.p l , 3,5 2, 4,6,1 2, 4,5 t 1,3,6 t None of the aboveis correct.

A. B. C. D. E.

( {l

\ is 17\ Which of the following statements TRIJE?

l./

A.

B.
t

n
\7

tl

D. E.
i 18.

if An organismis calleda homozygotrs it carries ? differentallefes, F must be expressed If the F2 generation trait in a l,:l ratiot the Fl' generatior't heterozygous that trait. F for two statesthat an organisr'n'inherits allelesfor any Mende|s Law of Segregation given trait, one from eachparent. ? g^ ccl8 r \ tflfg ( a !$t fiw<, how genes modffred.p are biologydescribes Centraldogma molecular of Some viruses storetheirgeneticinformationas RNA molecules. J 1 E_ for isNOT necessary a PCR experiment? Which of the following components dNTPs (deoxyribonucleotides)-/ Reverse hanscrip{aseX" DNA primers / DNA polymeraser,/ , Template DNA J

A. B. C. D. E.

l0 19.A man with a l0 Kb longVNTR on his homologouschromosome marries a 10. womanwho has0.5 Kb or 1.0Kb long VNTR on either oneof her chromosome What pattems of VNTR would their son possiblyhave?
,^\-D

A. B. C. D. E.

10 Kb and0.5 Kb 10 Kb and1.0K b 0.5 Kb and1.0Kb Bor C A orB

j 1ii

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20. Which of the following statements NOT true? is A. Alternativesplicingcanproducemultipleproteinsfrom a singlegenein eukaryotes. T B,M o d ifi cationo}@ mRNA suchas a d d in g o f p o ly A t a ilis t o p ro t e c t them from degradation the cytoplasm. K in upstream the coding region.Jof C. are The TATA box and enhancers DNA sdquences a D. sickle-celldiseasecontains singlebasepair The defectivegenethatcauses the change in its sequence. mutatedhemoglobin changes shapeof the red The blood cells. They arefragileand do not transport oxygenwell. T E. All of the aboveare nottrue.

/.

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chain reaction (PCR) has maj,orcontribution to the l. Invention of pof,yrnerase development in the areas/fieldsof: I. II. III. A. B. C. P: E. forensic sciences.r/ Fj/t {r'9{f.'dindustries',/ pharmaceutiaal agricult*re and horticulture lo n ly II only I and II II and III I, II and III

orderof cloning a humangene 22- Frcmthe stepsgiven below, which is the COR.RECT host? in a bacterial I. Il. III. IV. A. B. C. D. E. Transformation ) )* Ligation restrictionenzymes '|| Cutting DNA and vector with appropriate Selectionof colonies 1 I, II, Ill and thenIV III, II, I and then IV III, IV, I and then lI II, [, iII and then IV IV, I, II and then lll

'Is )

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the doesNOT correctlydescribes techniques, 23- Which of the following statement(s) the with sequencing human genome? efforts and effects associated A. B. c. D. E. and New techniquefor DNA sequencing assemblywasdeveloped.l -+ institutionsandprivate companiesresearch both the state-funded It involved I craig Ventor was the foundingmemberof the celera Genomics 1 andpatentallthe genes to It was intended discover biologyl prospects the disciplineof computational in It had createdhuge h v

cel in 24. The properties of a protein expre-ssed, the human' ls could dependon : f. ry'III: of The sequenae the gene that encodesthis protein The modifieation that oeoursafter its synthesis J\ Thc er*vironr'nent is in.1 it

A.
B. C. D. 'E.

I only
ll only III o nly tr an dll I, II and III ,

' luo'* Y.+Ni

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. \=

Questions 25 to 30 refer to the following information. Please read it CAREFULLY. with GeneW whose complete loss ol protein The following information is associated function is the direct causefor an inheritable form of lung cancer. The gene residesat the

humansgt chromosgme. thromosome& -<------"-

ft(CpjsJd'&-

the By exam.ining maturedmRNA and convertingthem back to the original form of DNA sequence (termed cDNA), scientists at the National University of Singaporehave discoveredthat all female lung cancerpatients carry fwo copies of the faulty (mutant) the allele (W') that has an insertionof Intron 8 between Exon 8 and Exon 9. This is due to a singlepoint mutation at thejunction betweenExon 8 and Intron 8. Individual female showing this intron retentionin only one form of the mRNA ig a carrier and is free from tnls cancer. The Exon 9 is the lastexon of the entiregene-ThematuredmRNA for healthyindividual will not havethe Intron 8. The sizesfor the Exon 8, Intron 8 and Exon 9 andaregiven below: Exon 8:2 kbp Intron 8: I kbp Exon 9:3 kbp The structure the cDNA for the mutantallele W' in that region is depictedbelow. of

\rdnr"rd \/'
Exon 8 Intron 8/ Exon 9 ./ P'AY1^'L-'

.&lcoRI

EcoRI

rnBMd "? c'At0

-&7

I 3"2 w\ld t^,,u"kqt: VrL

10

this type of cancer,the scientistshave identifie kit To develop a quick diagrrostia f'on region- for bolh vr!!@Be and gpgcifio ,"st i"tion Sitip for- EcoRJ pt"t"nt across that by the- vertiea] arrow-s above. Furthermore.,the region urlnafut"d fi"t*t;ll"lr*, probe for Southern'blotanalyses hGhltght"d by the thickenedlinl is to be chosen as the positions of the (Frobe Q) while horizontal arrows pointing to each other refer to the 't; reverseprimer (end of Exon 9) that they - io.waod f,rimer (beginningof Exon and the plan to usein anothertest.

1s.What is the most likely mode of transmissionfor this form of lung

A. E l., {/ D. E. Autosomal, dominant Autosomal, recessive Sex-linked, resessive Sex-linked,dominant Not enoughinformationto determine

cancer?

is/arethe expected on and above tested ttre@hat indicated 26. Usinethe primers for from Emily, a fema-iE-carrier this cancer? on si.e of th"et'6$ products the sample v no nZLd {b tU+ A. B. C. 4 kbp only I 5 kbp only 6 kbp only 4 kbp and 5 kbp 5 kbp and 6 kbp

rit{\wsl)(YP,|^^!-.{but not the cDNA, . IJsingthe primers indicatedaboyi4p diested on the eenomicDNA 27 sizeof thq"C$ productson the samPlefrom EmilY? what is/arethe expected

ffiE.

4 kbp only 5 kbp only 6 kbp only 4 kbp and 5 kbp 5 kbp and 6 kbp

L<o a/drr"'l
d

11

28.lfthe PC.pfioducts frorn the analyseson Question26 aboveis/are rg$@lggllb' enzryfoaififand then subjeotedto Southernblot analysis,what is/are the expected sizeft)of the DNA detectedby the Probe Q? A. B. C. ($ E. 3 kbp only 4 kbp only 5 kbp only 3 kbp and 4 kbp 4 kbp and 5 kbp

islareFALSE? 29. Which of the following statement(s) from his father who is suffering It is not possible for a boy to get this disease s4ty 4iv{ Y frsmthir form of lung J T F'ftfl^cv "uo"", Il .Th e r eis50%chancethatagirlwillg e t t h is d is e a s e if h is f a t h e ris s u f f e rin g J < Z from this form of lung cancerwhile her mother is a carrier for this disease III. lf the PCR diagnosisof the cDNA for a man showsa 6 kbp bandwhile the Zo Southernblot analysisof the cDNA as conductedabove shows a 4 kbp band, .., then'thereis a very high chancethat he would developthis form of lung cancer I.

A. B. C. D. E.

I and Il II and III I and III I, II andIII Statements I[ and III aboveare all correct V I,

30. Why a singlepoint mutationat the junction betweenExon 8 and Intron 8 could have caused disease? this This leadsto higher level of genetranscription This leadsto higher level of protein synthesis of This leadsto oompleteabsence protein synthesis productionof a mutantform of proteiV This leadsto the Not enoughinformation to predict

B. C. D. E.

12

annealing orl4 , Al. In designingDNA primersfor PCR anal5lses-, earlestinratethe general rule is based'on oafeulatingtheG+C contentsand, " temperhture for the reaction. A Celsius, while' A*T contents. Each G or C pair is estimatedto contribute te 4 degrees ld b'ethe nearestestimated 2 T pair contributes degreesCelsi each A or has the fullowing nucleotide (in annealing temperature degreeCelsius)fr compositions:

G: 5 nucleotides; '.t ( A: 5 nucleotides; "r. T : 6 nucleotides; t- \l C : 4 nucleotides.:i 'ir.i.

j\i,

":', 1{i?lx t
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B. C.

25 35 45 55 65

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32. How manytypes sf nucleotides(i.e G, A, T or C) canpossibly exist in a doublestranded DNA? B. C. V 4 only 2 to4 2o r 4

rr,i rtrdl' t
,.rr i ,r. i i' i' i ,,' tr
fr

ti

7.

Ecq

is/areTRUE for n1q!9gis? (f9)lWtrictr of the following statements IIIII. It only takesplacein gametecells T 4 one parentalcell generates daughtercells that are After eachprocess, a haPloid -,---------pairs of JranslocationFquently-bccurs between non-homologous chromosom.t:S-: /

k:

B. C. D. E.

I and[l lI aafd {\ III

I andIII I, II andIII Statements II and III abb( areall false I,

to

IJ

34. Which of the following statement(s) below is/areFALSE for the organization and function of a bacterialDNA?

I. tl. III.
A.

genes Some theirplasmids of carryantibiotic-resistance =y' *:Tpairsf' Theirchromosomal DNA hashonrJlogous TheirDNA is packaged tightly witnfri:tojllqjleins F X

q^lY ttV$'ttY _*^ , ' &t'tFT^4

r and rr
II andIII I and III l, II and lll tt.f*i-l I,II Statements and ill aboveare all fsF

*c,nL";,5y' errita*Yf

e{'tlFuw>..*

C. D.

b .fr,

nE.

3 5 . Which of the following optionsis a misrnatch?

,4_/-

A
87 C D E

James Watson solved structure DNA \/ A the of cloning J ?^ Stanley Cohen discovered method foX,PNA a CraigVenter ventured the t rr"n#ffipcing pr6;ec fi T into t Kary Mullis- invented PCR technique'l '1 the Griffith- confirmed genetic the material DNA andnot protein,l -y was I

"K9

Questions36 to 40 refer to the following diagram for the synthesisof a protein-

Growing pclypeptide

CA G

6s r
?l

--.---_-{

._"J
aar

Csdon Codon ,e<rdsn'Csdon, Codotl Gsdgn Codon

a2 8ab AE4 aa5 i6t' afli

\.r

LYJ v,

vJ.\/

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Mavairrrent of r-ibsstlme

4t tq

INCORRECTLY dcscribesthe mofeeulethat is 36. Whieh of the f6llo,wing statements labeledX?

A. B. C. D. E.

from a genebut cannotbe translatedto protein I It is trar,rscribed It can be recydled I . bondsbetweendifferentpart of itself I It forms h.ydrogen *2"1 bond,s with the moleculelabeled It forrns hyd'rogen It can cany diffe:9ry1ypeso{amino acid,but one type at a time F

the CORRECTLY describes moleculethat is 37. Which of the followins statements labeled Z? i&, oflgt

+'
B. C.

(except thatU is in placeof T) as the I (( carrles me This moleculecarries the exactsequence I nls vAl active/ternppg sirand of a gene fr ).urkJ^

iliffi;;ffir;;;;;;"';;;hAis

E.

but This moleoule only exisiin eukaryote not in prokaryote i can B can how mucha polypeptide be madefrom it Its stabilitycandetermine ., i) : It remains of the during process makingthepolypeptiAg mainlydouble-sfgrded i D

along sequenfe anywhere the take eJn place F ffi+

to of 38. What is the sequence the polypeptidecorresponding the amino acid number4 to number7 encodedby molecule labeledZ? [Referto Table l] "' A. Cly-Pro-Asp-Met. Gly-Lys-Ser-Val @ I i\.i i' ,i \r " u ,JI C. Lys-Gly-Asp-Val Pro-Phe-Ser-Gln D. E. Not enoughinformationto determine

39. The complex that is labeledY is crucial for this processbecauseit provides: I. Il. Ill. A. B. C. D. E. ' { the physicalsupportfor the process the iight environmentfor the moleculeX to comein contact with moleculeZ the originsof proteinreplication I and II Il and III I and III I,l l and HI tn to' rttcl Statements and III aboveare albaercet I.ll

15

<F-

t
I

9l:.5:

tst

(s) L Which of the following organelle(s)or cornpa,rtment in the-cukaDtotic_cell' doesNOT containDllA? A. nucleusj , . C B. cytoplasm N C. mitochondriaJ D. nucleusand cYtoPlasrn E- cytoplasmand mitochondria

doesNOT involve formation of Hydrogen (H) 2- Which of the following processes

-9gdsi/ i\. replication J '1,. B. translation] . '/

C. haverl uz:"* ,}tqtto^.f &v'dtvrt'/':J/ir! D . protein folding]*: *L*q, involve H-bonds E. All the above processes

;;;6;,i v ,'-l

3 . Which of the following statement(s) is/are CORRECT for the Human genome? A. Most of the DNA codes for Proteins \:' B- Most genescontain introns I V tr C . Exonsdefine where and wheh genesare to be ac$dbt".d D . Aand B E. Ban dC

.->

4. Which of the following cnzymesis/are NOT used for making a recombination plasmid?

A. Restrictioncndonuclcascs rr/ B . ligases -/ C . DNA polymeraseq D. Aan dB E . Ba n dC

is the chancof Mary's daughterhaving at least one copy of the mutated \Wf,ut alldle? A.25 % 8.33 .30 c.66 .7% D. 100% E- Not enough information to predict it

pVhichof the lollowing descripaions BEST describesthe health statusof Mary's her? A. Lteis a carrie4 hencenothing happensto h'irn B. He is free of disease as he carriesno faulty allele of the gene H C. He is going to develop the disease the allele he carries is very likely to mutate as anytirne D. He is likely to havesufferedfrom the disease E. llis health IKRefer to the geneticcodon tableprovided in the appendix, and assumethat fuqA rbplesentsone of the codons in the gene,what effect would such a mutation have on dreprotein it encodes?
V

A- Itreplaces one.anfinoacid with another type B. It lengthensthe protein C. It shortensthe protein D. It dses not causeany changeto the protein E. No protein is expressed all at

1l- In the cukaryotic system, complimentary a or:op_yO_*{frPUa/"arries the

same sequence information as its matured and spliced mRlfA of a gene- The lengths for the two exons and one intron for a particular gene P are given as below.

Exon l: 267 nucleotides Intron l: 1003nucleotides Exon 2: 603 nucleotides A- 269 nucleotides B. 1003nucleotides C. 603nuoleotides D. 870 nucleotides E. 18.75 nucleotides

{ 14(N I

iu\ {to3
for mitosis? '"F,ffi?@yilil"rftffi"
t

12.'vvhich rollowing ofthe

A. It takesplace in all types or"LiliF B. It generates daughtercells that are identical T C. Theprocessof recombination occursbetweenhomologouspairs of chromosomesp D. Aa ndC E. Ba ndC
13.tMhich of the following descriptionsfor the DNA structureis correct?

A. It is double-stranded withan{i-parallel lrelixJ B. It is a polymerotfiu"ril-tnrdint"a fiom onenucleotide thenextby to lingk@l*\ Sct/rlilhWr| -tt *..{ w1\r--lrrwr tuirclu qrhosnhodiester polymercarries nbt(i6sit'ivb a chargedueto the charges itsbases in \Th"

f->\ \Ng*$-

D,.A and B E.Ban d C

14.Which of the following types of RNA is NOT involved in theprocessof translation? A. Ribosomal RNA f B. Transfbr RNA Y , RNA J C. Messenger D. Small Nuclear RNA E. All the aboveinvolved 15. Which statement(s) is/areTRUE for the synthesisof proteiFs in cukaryotes? by A. This is a deooding process'meditated H-bondingbetween IRNA and mRNA T

B. Proteins be modified aftertranslation can I a C._tsN$ ctn be translated'into polypeptidep

D - Aan dB E. Ba n dC I

does nothave its truehomologous 16-$Ihich of the following human chromosomes oounterpart? A. ChromosomeI 21 B. Chrornosome C. Chromosome13 D- ChromosomeX E. ChromosomeY

)Air ,.l

Question 17-20refers to the following paragraph: The following figure represents the linear restriction map for a gene with the restriction sitesindicated by Sl, 52 and X, generating 3 fragments A, B and C. The sizes of these fragments (in kilobases) are indicated in brackets. A specific probe (made by radioactive labeling) corresponding to a region that covers part of the fragment B and C (indicated by the thick line) is used to carry out hybridization after that recognizesites sl, 52 and X. Southern blot and DNA digestswith enzymes at 52 site (that would destroy this A pointggtatign-in Grt* Mrecognition site) is suffiFent to causethe disease
..) ") \l\

\'g ,.L,
B (2kb)

+r

M? is J?/. What mode of geneticinheritance for the disease A- Autosomal,recessive B. Autosomal, dorninant C. Sex-linked,recessive D. SexJinked,dominant E. Dominant. but cou,ld autosomal sex-linked be or 18. During the prribing, how can one increasethe speeificity and accuraeyof the 'detection process? / A. By increasing the temperatur e/ B. By reducing the the temperaXre ,, C.Byincreasing the degreeofhomol+gy of the prlpdto the target D.Aa n d C E.Ban d C

19. The map above represents map of the single wildtype allele M- For an individual the who is heterozygouson the locus (with one wildtype and one rnutatedallele| what would be the fragment size revealedon the Southem blot after the analysisby restriction enzymesand using the sameprobe above? A. 5kb,2kb and 3kb B.2kb and 3 kb only C.2kb only D.3kbonly E- unknown 20. A similar Southernblot analyses a person's DNA showsthe presence a single on of bandat 5kb- Which if the following statementbelow is TRUE? , v / A. The person is homozygousat the locus M V B, Theperson's child is definitelygoing to get the disease,, C, The person's father is suffering from the diseasealso 1 also / D. Theperson's motheris sufferingfrom the disease E. All the above arecorrect 21. Tralggjptiou is a processof malqng A. DNA only B. mRNA only C. rRNA only D. IRNA only E. mRNA, rRNA and IRNA

t r 't-

L/

DNA has a $ftosonecompositionof l57t what is'the cor'nposition 22-lf a single-stranded of its adenineRr-

A. ts%
B.2 A%

h- -f

c.25% D .3 s%

dc

E. Not enoughinformation to deterrnine

below correctly describe(s) the organization of 23. Which of the following statements DNa in various species? r-Wuffgg tyr"k) genomes very compact and can utilize different Eaffe"sfor synthesisof are A- Viral different proteins T

,t@fp,Fl**ogenome *linty consists B. Human ' chroFosome's in bacterial 1C. Histolfrarefound

D- A and'B\

;.;ff;

uor.n* u*u$ fl*\"t'

\A

below correctly describesthe relative order of 24. Which of the following statements organization?(< sign denotessmallerthan) A- tissue< cell < chromosome< nucleus< DNA < nucleotide B- Cell < tissue< nucleus< chromosome{ DNA < nucleotide C. Tissue< nucleus< cell < chromosome< DNA < nucleotide D. Nucleotide< DNA ( chromosome< nucleus < cell < tissue E. None of the above is correct

25- How many types of chromosomes ar there in a healthyhuman ovum (egg) ? 4 .27 8 .23

c.2 4
D.46 E. None 26. Thepropertiesof a protein rnainly dependson: of A- the sequence the arnino acids { B- the compositionof theamino acids fi D- Aan d B E.Aan d C

it c. theenvironmentis in h,.,f,Wtnm* /h,$r^rphil.r"

r
V

in from the 35,000genespredicted 27.More than 100,000proteinscan still be generated of because the humangenome

I
f'

A. alternative RNA splieing 1 -f B. modifi cation of proteins after their sy.nthesis C. geneduplications D. A and,B E.Ba n d C

28.If thesequence anmR\A is 5' ... CCQCCd,aLlooo of I I correspondinelcodine setififfiof theDNA? |

3'; what is the

\

'f

\__:+"

ANR i, TTTAJq.AGGGCCC...3' a G(,,c,[cc({rrl 33$33ffiffi::1; C. 5'...

I

.r3
ft,/

D. 5'...UUUAAAGGGCCC...3' E. None of the above j

29. From the question 28 abovo,what is the template sequence wherethe mRNA nu{ .beensynthesizedfrom?

'.\
jllnr

I
l

D. 5'... AAATTTCCCGGG...3'
E. None of the above

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30. The process exchangingtwo regions of ofnon-homologouschromosomes alsl is known as: A- mutation B. translocation C. cross-over D. transposition E. noneof theabove

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rsuraoz_s11,/GEK1s27_sll I tsttsozX.2rcextszz _stzJ

ceRs & $sciety-Slt-Revis eu$$ons'.gtt t t- t ./ ttt' il{nlber of Attempts : I cot or litJrnrtc Ouratlon: 17ir1 52s lolsl l.tffrks i 15 sui 0f 20 Assessment Title : Gens & sciety...5elf-Review I euesiisns-.Set studsrit Matric trtc. ie staft Fat asd Time :

^ \_/

stior 1 1) Seje{lire [rr'*ei*nlt is:

-F

a. :'' a. beied on shs*sing indiyidunt gecs that encqfilo.abte ph*notyp6. E. : B. b$eed on {hsosing Eiyorabt plruwtypc fif-.g}e nc$ded by parti{uta, gens. C. C, br$*d ou cho6$irq fflvorable ervirorment that mry iilttuen* trvorable phsrctyps. o. I tl. htred os choosiilg fauo.abls genatyps that en@da for favorable phenst?pg E. : f. nsrre 0f th* shove is covert,
S, B. basecl cn chssins fivorable phrotygs phenotyas tlai

elkctisrn t3 ., *r,,"}" nmturral

nt,'l r" "' !-*il|\f-'*'{ yg4,rorn hr/r

K$_rnr1,

Corect Anfwer i 3. based cn chmsing favcble Y.our lularks r IoutofL

thai may be encDded bv nenicelar nenes./ fiay be encoded by panictrlar een*.

Df,lri are irund ir: ihe talloNvifig comircnenls/oiqanel$ I. aJaleJs. Il- 6ii*h.iri,ii,a. llI- chlsigplastl!'. qclgi igf-rar8tir$. A. B. c. I and 1: *nly. I and $I *nly, onb

:n llie {:e!l

I. It snd ilr

D' E.

t, rr 6nd tv onty, I! II, III ANd IV, !,cur Aeswer : :. l. Il and 1I1 snly{ Co.rct Acswer i , il and lll only Yo{. f'lafks r 1 orit 0f :

'fhe pxrcess oI'lrnnslalion re{luiIts the ltillowirrgt

A. B.

. &. *NA t*mplate, ONt polymeB$es, ail *JtAs and amtno rcids. :. B. DtlA tsFl*e, tHA Sclymerss and t*Tp$, C. riboslme, mattA, ttHAs asd t'tTp$, D. rRHA, tRilts, ffRt{t an.l N?p$, ' F. .ihossfie. mRltA, tnNA aFd amins acid$,
vgur Ac$wgt ; Coarect Arsser , F. rib(Eosr, fiRNA, tRtid. *nd irr;lo , riboicme, mRF{A. IRNA and afiiro acrds.C acids,

c
D, E.

Ycur Marks: loutofi +1 Which of the following statements iVare NOT true for mitosis? a. A. CD' E. place in alt tytes of cells B. tt generates dauglrter cdl$ thrt ar idnfical, C, The proces Invotr* Kombinetion heiw*n homologous paiK of c|rromdsmeg. D, a and c. A. lt ta*s E. B and c.
'rbur Afi$lver Coirtrt AnsBer I ). $, A al:d C.if A sniJ C

\,

-ubur MDrkri

ort of ,

s) lf rhecodingsequence a DNA is 5'...GGGCCCTTTAAA...3';what is the of conespondingsequence its template? for r Bc lL

E-

A5'...c6cccrrraAA.,.3' B,s'.,.ccccuuuAAA...3, c-s'...TT?aAAGcctc,,.3' o.s'.,.uuuAAAGGGccc...t.

[- a*rre o, ttE alrove.

c. c. 5'...lTrAAAcccccc...:,t'
Coirect Arswr c. 5'...iTIAAAGCCC|C..._1',

Yourl4*rks loutofi
6)

Tlc rext four questions are realted and basedon the following paragraph. friseaseT is a form of heritable kidney cancersthat developsduring the early childhood ofan individual. It is causedby the loss offunction for a protein T that normally works as a turpr srppressor.Scientistsdiscoverthat the exon I ofgene T in all patientssuffering ftmr this di5sasegarriesa mutation from the wildtype sequence -. /a.{A@.ttla"5' ._ AAAGCGACGGATGAGAAG... to 5'... AAAGCGACGGA6IGAGAAG... .3'. .3' Kerin aged30, doesNOT developkidney cancerdespitethat one ofits allelesfor geneT caris a mutation as depicted above. $'hr is the mode of such mutations?
t lA- lnygsicn. B. tn*rtion,

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c,
D. E.

C, Detetion. D, Duplicalion. . Unknown

Corect Aiswel Ycu. Marks

C. {. Deletior.lf, ). Inse*:on,
)o$tof1

?) (RelatedQuestion) Kevin's wife is free from this disorderand diagnostictestsconfrmed that both her allelesT have no such mutations.What is the chanceoftheir children gettingthe disease 7T in the future? 7L

A. : A.os/o.
B. i: B" ?5o4.

7t

X-*

c.rc.'ocr'o.
D. E. 'r: B,?3oa" rii f, l00ols

rl|
I '|l Your Ars!9er I :. C. Sttq".}( Cdireel Ariswer 4,O'rc,

W .

y
.

Your Flarks I oul of :l

sl (RelatedQuestion) What is the chanceofKevin's brother having one copy ofthe mutatedallele?
A, B. C. o, E' 'ii t'. 2$o/a. :' 8, $Oole, .:. {, 75.tn, D" loco/o, r" E, Hat nough infsrmatlotr to Fredi1. E, R. t,iot boilqh intoffi6tion i-o predict./

Core(t Answer l :. Nct nouaih ind).rnatior to 9*1iit. v.cur Mailis : lartofl

e) (Related Question) Which ofthe following desriptions BEST describes the role or properties ofprotein a. :: a" $rildtype prstein T is found in kidney Rncer cells^

T?

B. ' B. l,lutant protsln T i9 nct found In Kevifi's kldney alls' c. ': C, Mutant frotsin T ciuics formatlon of canrffi only when itF wiltltype cdunterpart is absnt in cells. i* prffitt D. ::- D, tlvitd tirpe Frotein T partlally iltrffi ctl gswth and froliferstlon whs its miltant $untrFa* E. :', E. flone of the ebove d*dption is aortect.
D. $. lrJild tyFe prctifl T n I caus* farsation

lf alls,

of *ncen

cou[terpe.t celi crowih ard o.oliferction when its filtart arlY whetr ;ts lvildtYlH cotJoi'srpBit is ahserlt In c*lis

is presert in cet;s X

10) Which ofthe following statements below correctly describes the relative order of organization? (< sign denotes smaller than) A. :' A, Tlsue < celt { hrcmsme < nuclcus < Dt*a < nulmtids B. C D. E. B. cll < tiss{e < ntrcle$ < chrofioffim < DNt < nucldotide. , c. Tisue < nuclew < cedl < hsmo$m < Ol*A < nuclmtide. g. Rmlmtide < {hromosome { ONA < n[cleus { cell < tisse. E. t{otre of the lbcv* it In the corrrl srdr'
E. None of the above is in the cenei . N6ne of lh above is in llie cortst order'd

Y.0uf Ais!.rer Ccirect Ariswet

ofder.

Ysur Markg ; 1out0f1

11)

If the non-templatestrand of a DNA is 5'... ATATGCCTATAJqA...3'and it containsa start codon, what is the two amino acids that is adjacent/nextto the amino rcd codedby the start codon (refer to the geneticcodetable from your notes)? I- Ile II. Leu III. Met ry. Pro V, Cys
A BC D. C. - A,3 and XIL B. tu and rv.

.IandV. D. I acd nt. E. r: snd V" .*, il, I anci iv.f/ Coffect Answr : ]. I ard IV. Your l4erks out 0f I

tzt of 141t+ determtr*<!thal l&e amilu*tpf pr:rirreis equal td the anrdrunt pyrimidite in a dr.rubl*sfiand$dDNA'?

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A. B. c, D. E.

A. friedrlch B. Phosb*s C, f trin

t{eisher Levine

Cltargaff

B..]!m6Watsn g. Frarcl$ Crlck

I
ft,

Which ofthe following statements below conectly describe(s) the organization of DNA in various species? A' :' A, Virat Snoms are very ompact and can utilice rverlappirg frem*g for gynthesis of diffe.ent Erotire. B. '': *" liufian grome mainly consists of non-6ding *qsenc*, C. - C, tlistons ars found in culGryotic (hroHosoms only, D. :rj Er.A,and I snty, E. ': g, *, n and c,
F. ll, S end C.{ foire{t Arswel A,B6ndC You r f'lerkS 141

loutof:

lfdrringlranscripti*n, A'

* sirttle *ui:ler:l.ide i6 *rris{*Lix}l.vcopied 1|} a dillbrent nucl<;otid* {substitoticrn;:

r: A" the tuarti$4 or itrustute f the prateln pruduct will bg chtngsd, B. :' a, lhr fuilctio* or structure of thc psteln prcdqct may dr miy soi b chang{. . ':: g, 11r*nunotype witl bc f,lrnrged, b[t ttre photrotype ilay or may not be changed. D. :' D. the st.rcture of tha prolei! Frcduct will bc changed but the fumtion may or may not hafiged, E.':: g. 16gr**r*ype snd the phenatype m|y changed.
B, the fuactisn or $trlctu aoirect Afrswgr tic! r Merks loutofl of the tietein treduct be cherqed.d lhe function or strilctur ct the tietein troduct nev sa mav rct be chs*sed.

!Vhich *f the lirlb*'ing correttly describes the diff'erenoe{s) betu,e*n DN:\ and R}sA: I tlnc ol'1he pvrirn:idine base i: rIil.fbrenl. Il. Urre of the purine hssa is dit'lerenl. if l. --lle sugat is <liil'*r*nt. IY Ihe plrosphale is drlferen{ A. B' c D. E. A. ! and III 0nty. E. aI asd trI anty, , c. r. ur and rv anly. D. Ir. ttt Bnd rv onty. E. I, II. ItI and IV,
Y?uf Answer lsrst AFswer Ycur Marks \. A, I ind tft onlv.rtl I anC ltl orly. lout0fi

y 'xt
wiici or ths lilllolviig siijterneFis is/ere true? l. Dlnog DNA .eFiicaiioi, cne strtsrd is rud in a 3' to 5' dircctlan ard arother stnd ::- Drring transffiilli$n, the {:odi!19 sird is ie?d in 3'tn 5' rjirtrJion, :lL i,u',q! translairgn, the mRNA ;s read ,t a 5'to l'Cire(-tion. -/ -r iudc9 t.ailtiatioj1. the rsiyoepti.le charil is 5vnthe:;r?e in I' tdff diiRlio{. /, is read in a 5'to 3' dirtrtion. .r x

A BC lL E.

t, III onty. 8. tI ang It: cnly, C. t II and tII only. B. tr. trr a(d rv onty, g. r, It rrr and Iv. >. t). I1, lii and IV oniv.X
Coirect Arswl \,111 only. l'our f4e.ks ; icut6fl

1t -. -- ----**3

I\TKNR

17)

Which ofthe following statement(s) is/areTRUE for human(normaVhealthy) cells? I. All gametecells (mature sex cells) are haploid. II Somaticcells have at least22 pairs ofhomologouschromosomes. IlI. Somaticcells have the samegeneticmake-up. IV. Gametecells (mature sex cells) have disimilar geneticmake-up
A. B. C D. A, I and II 0nly, B .n a *d ItIo n ty , C. I. tI frrd ltl only. o, Ij ur pnd rv only

E. : E. I, II, TTI ANd IV,

Y.ourAngwer l \. A, 1 anc 1I orty,*{ Csryect Answf : L ii. III;nd:V, Y0ur fq8rks i i"!tsfl 18) Ti'e Cntr$l DiE.na Of !ts:m:u:ar Bisi$gy Ces{i'bes

3 of4

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A. rrrA. l*w genetic lnforildtion |3 trtnssitted In the cell. B. ir 8. how gffiftic informatioft i3 arganlted in ths celt. C' : C. how gntic isformatios is clEnged or mutttgs ln the cell, D. 1LS. how qenti{ informatioa is Srcdured or manuftctured in tfte cell, E. 'l E. none of th aboy& i3 (orret.
'{otr Aiswer i 4, how genetic infcrmation is tnsmrt{ed in tne cdl.d

fo{ect Aasws : \. how genetrc intcrmation is tnnsmitted in the ce!i. Yolrrl4arks cut 0f 1

re] Which of the following statement(s) iVare FALS regardingthe humangenome? l. Most genescontain exons. II. Introns define regionsbetweentwo genesthat do not code for protein. lll. A diploidgenomi is abouEli[dfiiej-pairs. lr.fr-. IV fie estimatednumber of genesin the humangenomeis about 35,000. I t - l(F a. 'r A, !I and trl only, B' ::rF, It ard tv only, c. ':rc, r, flr ard rv onty D. :'' D, II, tll and Iv rnty. E. , F. & tr, rxr asd Iv.
i, D. 1I, iii arcttV orrly,d C$.r&t Answer
v, r l"larks cu II, Ill and Iv only. lcutofi

201 Which ofthe following processes involves complementary base-pairing?

A. : t.OtiA Rc$ilcation" a. L Trnscription, C. l: {. T.anslaticn. D . 'D ,A a n d Ro n ty. E, I' E, A. g and c, , E. A, S andC.d Co.,ectArswer E. A, g afii C.
Your l'larks lsutoft

:,-=

*i!

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LSMl3O2_SL1/GEK1 527_SL1/lsit 1302_SUl/GEK1527_SL2 G*ns & Ss{iety..Felf Review qse6tio$ S'et II

ti
Astsilent title : Gens & Society..Sll Review Qusticn 9ludent Matric ilo- : lstart Dat snd Tiile i;-F :*rrien 1 St ll

Nlsber cf Attempts : 2 cut ol !nl:iuitild guratior! : :m $,is Total M*rks : .1{ioul 0t'20

r)
i-il{ [*rre{1 srqu*$c$ lbr r:okinrr hvbridi;*rtjrin ex}erimenls ir. A. It. l) ll. lr. A replica cfthe bacterial cobties is cbtaited on an alxorbent disc. Au1fi"di*gr:rphv {rl-the dis* r*v*nls prob* o*mplcrm*ntarr- },}NA. ll$-\i bactena $'ith plasmid are plated and alloued ta grorv overnight. Jhc lucteria a.relvs*;l and rh* IINA a1{:d*narirred on the disc. -l-he di"scis reactcd nith iabrled pnrbe.

,d,{t, n, B"rJ {1,A. [, I], B {t. ti, A, n, a.}

{.ri A. l:. 11"} ft, A, ti, g, B

:. C^r\. D. E, B
Ycur AEsllc, :

Cirect

Acswef l

I] , A,I), !:1.

Y.ott Merki i 1 cut cf :.

I rr r linuilil a lpl i:l+ctr'.rphilresis to .l$:e nnifie the tizes r:f sr:rme DN.{ lialgt,ti:ntli l ve 1rilr'.' i. This unknswr f.Bgment apteaB to be Jrst over fOS bp. , L This $nknown frasment np$*affi t$ ba jsst unde. 2(t0 bp. C. ' *, This uilkr$wil grasment is nearcr to tlle n*gative el#trod*. B. D. E. D, $ the bands ds nct otcur in exa{tly the sme tffitionf E. N0ne of the abc*s is corrst^
l. B. Thi5 ilrknovrn f.aqment atfieis Corect P.rs!*r : ). ThiS urkrawi fraonnt Your Marks i loutofi ir be jl:rt tnrler ?0.3 .Jp. d !ndsr t00 irc.

A.

t cannot mrun*

rnythinB tbout the si:e st the [nknown f.agmeilt,

tscBeari to be ilst

3) A. B.

Spiicing rd'ihe nrllNA : t. cccsrs ssly ltr ukaryot6. g. can occu. siffiultaneassly du.inS translation of RtrltA. C. tan oacsr in tht nucleus oa (ytcplasm. D ,ta n d 6 o n l y, E. AJ A nnd C,
\. A. #iilr5 {:o.rect Af;swer i {ailr! aut of i gnl'i ir silksryoies.}1

c
D. E.

oLwin nrdlrrl

cn,l ir eukaryois,

Ycur f,1ar'rs :
c; I\ L A. C D. E. 1r't> nrrmr:l ierull erlls sirlrih*nt orsiv

*ndergr

nritosis in a siaEle ilrgan;sm.

irill be: a dlff*rena gsome,

a, 2 datrghae. cellsr each with B. 2 daughtei c. 4 dasghter o. 4 dauqhter F, I daught*r

cell$. wlth th* identiGl *ells. with {ells. ffih

gtroms, to either that of the {irst celt or th.t genof,e. gemfie, of the ffcod cell.

a genotsre idehtlcal with the idstiffil the identil

eell$, each with

Coryst Answer :

). D, 4 eidurjhter ce,l$, each wi$ tile jdeF,"icsl Sun,urr*.d 4 derohtei cei16.*ch erih rhe rCenticil

Your Mirks : lcutofl

li'h;ch trf Lhc iirllottiir* is true r*ganjin,e lranslalion? ! in cularrirles ii i.rci:ursilr tlie {t lop}asrn. in rrokal,trtes it tr:curs in the cytr:plastn. in euksnotes and prdialvr{es il ocr*rs iri cl*se aisi:{..ia1ior} with thi-1 1trlvribosrrme. !n eulanotas and prokan'otes it occrrs onlv n']rcn th; ntl{NA has finished pror:esslng.
A B. cD. E. A. I and It enty, B. tE! and IV snl?. C, t, II and IIt D. t, uand E. I. !:, l:I only.

wonly, and Iv.

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Afswet i C. I. r l ar d III only.d i:o{srt Answer :. l, ll a$d ill +nly, Y.ourI'larkE I sut of : 6)

v,cur

You rysnt to rcplicale a piece cfl]NA in ordi:r irr have a large quantitl. tiri analysis. !\"h*l shoull b* the o{}rrNrci socluen+*.,coLrrsc. ofvo*r ac$ii:ns? A' B' C' D. E' a, Transform bactcrial {ell6' seltrt clones bv blre/white and antlbi*lic resistancs li*ate lr*o a vector, nnd extracl thc plasmid DNA feom ilre bac]terie, B' Transfs.m bactrial ells. wle$ ttanes bY blG/whit and anttblotlc rsistanc, ligHtr lr|to a }wt{r, and e*rart the senrHie DllA fros th ba(teris, {. Lig*te into the genofii stta, transform bstetlal celk: slt clsns by btuelwhit and antibiotte resistarce, strat thc pksmid frfia frsfi ale bact*ri8 D" Llsate i*to B ptaseid, transfotm bacterlil cell+ *tect clans by blse/whitf and fftlbi$tic rststnffiq ertfa{t the Ftasmid Dl,lA from the bacteria. e, ilone cf the aboye ts rorrst.
I

. t. l.igate ixio a plessid,

tmnsflrF

baLleii*l cells, sele.jt clqnes bV hir+lwitii

snil $rltifiiatj(

Coirect Answr :

). i.igate into a plasrflii, tian:fcrft

ilacleria! cl:s, siec dsres

by bllelwhite

ard 6ntib;ati.: resistarce, extis{:t lhe tjlaajrnid i-\iiA {(rrn the

\.'c!r Plerks i loutofi ?) \'lx11

' lrasa diseasc ihi.t is i:aused a ftutr{tioniil +nerrfher gen*st}ial is sexlinhed by

i, tter sn wlll rlfinitly get the di*ffi. gee ihe diffi*. their d*ughler will dfinitly rot *e a ranier,

anJ !s r;ie:;sivs.lVlri*h oi'1hefbllnrving cnrrer,1? is A. B,

G, tler daughter {. If hr h$sbaftd B. Regardles

will defi*itely doB

c.
D. E.

nst have the diffisr

sE ttr husband, is csrrffit.

any child of herc hrs a Soolo chasce td get the di*ase,

' , uone st the rbcvr

\. A. Her $on wil, detiF'te:y get the Cissss.d Coireal rlri$wer ; Her son wrll defrn,teiy {e-t the diseese Yur l4ark9 : loutef!

al is lhe d*te*iive tuvrlsti{.tirtir}I, ori!:B*_ d },!ru ar* *rtereslecl l{t see iilhe DN.A i* Lii* irair tbilicic ieli at the srdna lratcllds an.v of the three prine suspects.lJnknorv* tn v*u. one oI the susp:*is hls hari a trri*rrerv lrnrsplarrt. lVhir:h rf tlrr,rt'nlloll.irrli i:l conr:crl A' A. c A. taane $l ttts Df..i tlngerprintitrg tests wilt bs vfrtid. B, The DNA fiftgerprlnting tests wifi bc yatid rgardls ' e, RFLPwg[ld he vdtid, hrt Fot VNTR"

ot his tratrsptant.

D. ' D" VNTn would be vfftid, but not nFLF, E. E. None of the absr* is corrst,
:r{ tests will be valid tsts will be v3lid fecardless cf his

9] !'I:i'vinlltt}ii5ef13pie.reDN,4i*loap1asriidtectorrvjthalccijarlantii]i$ticresistanc*gen*t.tr aB. c D' E. silty - all cf the whlte mlonis will definitely ca.ry the oua of intersl, only, all of the whit c*lonis will dfdinitely {*rf the [rNA of inter6t, c, r need ts uR both atrtibloti and blue white *leetion to ensu.e thst all af the whlte cotonies will deiltrltely arry the pffA of interes! D' tt t nat da not {se itkr msthod sf slecticn, the cslsnie$ carryins the Dila st lsterst will be b!us, B. !t ! !$e sn*iblotic *lectisn E, n$ne of th aboc i$ c0rrect,
:. i. I r&d C0ifect F.ns!.Jef Your Flarks l. I red lsut0f! t! $5e iloth antitiiotic ancj blue white selecticr t ensv! thnt all 6f th riirite rolsnies riii, dliliielv cirry th* Dt{A ri :.1.:rc- i // ts use both antibioiic aid blue white seleC{ion io ensuie thEt sll sf tlie wiriae colonies r,,iil definitly r$r.}, tie DNA of ilt.rest

A. ;f I lse btue-white sltisn

1r) :o*hen biottingrequire-r :

: ?.g,tn.ii$n enzvne {$VdiEest of Dl'lA, :1. Gei 3!ctisphnre:lr. :::- Pfq3;nE cf Df.JrilrnErrlelts. : j Aililliia3ticn cf DN,A figtrent9.

A. Bc. DE.

A, 3 and :I 0nly B^ ll asd lXl onl?. C. I. Il rnd III D. II, Ili only,

a*d IV snly. and IV" {:. C. l. Il aicl lll only./ Ccir?at Atswer : Your Merk5 I. II 6nd lII only. I out of :.

E. I, II, ttl

111 Rjharir the anticodon (Meti tRl"eft{ the cod*n tahle)',' olrnethionine a.{. s' AllG "3' B. c D. E. B . 5 ' G 1 J AJ ' (:. 5' {.'Ali 3, I). 5. &r{i 3' E. lioni: ofthe abrx* is cerrect,

c. t,' 5 ' ,{ - ' At.:3 ' d

d0ireat Answr :

r.l.i' r.lAli 3

k$o,cr

fr"o

IVLE Assessment

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YourFlarks: I I cut of i t ?l Ih* i'undementai difterencej t?etween RN.4 End Df'lAni*: a' | .d, the organie basesonlv

B. - Fr, h*$e$, silg*r utrits, and the phosphodi*sler linkage c' ' tl" b*s*s, sugar unitso and the glycoridic bond typr o' ' lt. brser *nd th* sugar units n*lv E. .i Ii. base$, sngar and the phosphato units only-

u. D. halesandth* sugarunitsonb C
Csrre{t AFsBer :

D. bas*s;:*dth* slrliar**its o*1.v

w,cur f"larks l lautof?. 13) A' B' C. D' E. '$thi(ir ot the filllow;rq stsjternents corrtrtly , A. Thef work ' B" fhey on boih Dt*t and RNA, a paliildromic sequentr c$t is alwas atrd cut just bgfsre the palindromia blunt ended. lrffi fmm Hch ef,d, sequence, !deriifies a icstrii:tian ndilnuclsse/enr'rne?

rmgnlre

C. T3!e rsult of endonul&se $, They degrade e. tlrey

St{A by $shqquef,tly with

removirg

rut SilA only ad sits

spe{itlc

nrctestld

*qsencs.

i. E. :hey cui UNA orlv at siieg wiili spff,j{rit nuciwl.ide;*qu*"a"s.f C0irctl Ariswgr Yi$r l"tarks t4) A. B' C. D. E. \iJhii:r ol the fcll6w:I9 gtatesietk A" Some Gfthe g. Lsg larsest gs*oileg :. Thev aut Di\iA o.ilv 5t sits 1 cut of :l !9lare trre iegatdlig genomes ct varigug orgoi!:,ffi-cl witil soeclii.r cLileotide seoden.+q.

belon$ ts plant aild amphibians. genome cods for a proiein.

tben lOVq ot the huma*

{. A plasmid

ls not part 6f the hacterlsl

chmmosme.

-, F, A and f osty, E. A, * nnd *.

F. A, B and C.d
aoirect Afiswer Ycur M?rks : 15) .A,BandC,

lcr tsf:

Which of the following descriptionsfor the DNA structureis TRUE? IIL lII. IV It is double-stranded helix and the strandsare parallel. It containsmany nucleotideslinked from one nucleotideto the next by phosphodiester bonds. It carriesa net negativechargedue to the chargeson its bases. It containsboth major and minor grooves.

A, , t. x andrI Gnly"
B. cD. E. I B, It a*d IV cnly. : c. t, lI 4nd rv sf,tY" ", D" l, tI and IXI 0rly E. I, II, ttl and Iv.

8,9. Il and tV ontr,.Nf

Corre( Af-qw*a : B. II arld lV anl),

YourMar1,6 lcutof!

rsl A circular plasmidhas two EcoRV, three BamHI and three EcoRI siteswhich are loacted at a distant from eachother. How many stainedfragrnents/bands do sou expect to seeby gel electrophoresis you completelydisgestthe plasmidusingboth BamHl and EcoRV? if
AL c D. E. A. Fout, E. Five. g six. D. sven, E. Eight.
Ysur An$yrlf Correct Ansller i U. f ive.

Y+url4erks 1 out0f 1

t7l ': r- t:ll to c{xltjrm if v$u havcsucces i Perli'm RIrl,P ou the [pn*mii: l.]NA to detecrrhefrr*:igngeue . l: ?erlbnn IrCIR plirnersrhatt&rgets f-trreign usingspec.ific ge:re. the lti ,'-' --iomSrrulhenrhkxtingusirigspeciiicprobetiralilel*ctsihefor*ign5r:ne. Per'r-onn IINA sequencing oflhe entireeultaryodlgano:ne locaiethe fbreigngene. tc
I a C IL B- It E. IlI and lV olrly, A, It ard w of,ly, B, II aad tt: c-& Iti crty

and Iv o*ty.

E. r, tl. trr and rv" ). ts, 1I anii III silltd Coirccl Ariswer 3. iI dnd lll s4l'/ Y,gur Merks : lcutofl

l**09 I

,**

IVLEAsspssment

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1S) Duchnne'ilusctlat d!,siro?ily, ans(iated a. B. C. D. E' , witi ibqve iegcriiticn?

a. x-tinfted. B. x-iractivation. '' C, t{on-disjsncticn

of X-Carrsmosm.

,i: D, * *nd I only I E.A,8andC. ), t, 11arid B oEiylf (:orredl Answer: ).4 $nd B anly Your Marhs : 1 olit of i

13.! !!h:i(h *rr.rle$uli:t i)k Nrxljiem bh:ri:s used to it?ei:ti&.? A. ,i, &. *f,tA. B. : B. nfiA. C. '. c, Frotei*" D. , D ,a a n d B o n l y, ' -E .a ,6 a n d c.

l. g. nnn.#
Ca.ict Answer i

). Rtia

Ycur l"lark : 1 out of i.

20) 'llre F{)ll {pol1'nr*rase c a hi:rl sFrjng insiile Yelloq.slrin tt !-unctior even olter it has b*en healed to 9j deg:e*s f . Wh-v xruld such a heat slahls p*l.vmercse be i:ereficial irr tlCR',? a. Bc. D' E. (95oc), which atlsws the primes tc annetl t* the ta.get DH,A. a "hot" de*atu.atisn phas qgSec; whictr sewes to sparate the primsrs frsm bindins ffich fther. {. Effih cy*tq includes a "bo*- denaturafion pbffi (gSbC}, which activats th. Trq pctymem*. P. rach cYcle i{cl{d# * "hot" detrat*ration phas {tSoC}, which *pstffi the t}}drogen bo.ds th*t hold tlre strand$ af th teflFlaie ONa tnfieth*r" ' 6, &6ne 0f the [bove i$ correct, B. Fach *Ycle incl*ds
Ycur Ars$f : ). D. Eijch c'lcle inchrdes 3 "h$i" denatsratioa phase (95o{:i, whh:il separates the hydiogRf; bofld:; thal h{tcl *}e st$nds osether,gd Cd.rect Anslver l. sash {ycie includs 0gernsr. v,cur Flarks : !outofl a "hot" deilstilististi thasg i95cc}, whi{}i sepaetes tiie hvdrog*n hot-.ds ths'. icld il-.e str"nd$ of lt* i+nip:ate $ii,4 af ir.? tqryliilni* t.}Nr\

A' gach cltcte inclsds a "hst" saturation p*ffi

a; ' : { }

@l
4 of l

[\l-E A.isessment

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lsM 13O2_SL1/GEK1527_SL1/r.sM 13O2_SL2/GEK1527_SL2 6srs & gociety..Self tr*yiew Qs*tio6 Set III Assesmert Title : Geneg Seie'.y. Seif ReviewQusticns & Set III Student Mrtric H0.: trIl start sata*d Tiile: Il-l
le{tian 1

..
htsmber of Attempts : 1 cut of lrliniied D ur ati oB: 138:s Total fi3fks i 14 $ut of 20

Whichr'r|.thelblltrrvingtennsisof1enu-<eeltoilr:scrihean<rrlirnisrnrlhi..h*airiesforeignI}NAin1rnrlucedfi,r.rnranoth*rirr5nisnl}
A. B. c, D. E,
A" Clone, g" Rwoffibinanl. {. Transgef,ic, F" Flybrid, g. fu$ion, Coarct Answer Tr?esqeric.

YourMarki

tsutof:

2) llrru'iloes gent-: tlt*rapy ilifier lionr lr*rlitional lherap;'ir temrsol'trealing1*uelicdisorders ii piiti*:nt? in

A' BA.lt lnyolvs int.odsdng ir|troduclf,g norm*l sstilal allels alleltr inlo the sm.tlc irto th* g$m cel* of an affctd indiyids*|. B, !t involvs cells of an atfftd indivldoal.

c. : e. if invcber transfrrring t<lrxr*l g*ne pr*aln{is in an affe*ttd per*n. D. '- n. a *nd B onty" E. .:,. A. s andc.
Coirect Anse/r : i.It invclves intrrduain{ v,o(r l'1ark-si loutofl normal ellel{s !nig the somatic cel's of en affecied irdivid[al

3] The lollowing rcqrrence {io!11 IDNA san}ples to bc targeted is b"v {h,vlrridizsd) a prrhe *n a nricroarruv chip: 5' .{'i GAztrACCCGC|]... ....]"ilCCC;\AACAC.. ..-?' ... tt\rted1:ne're{erta5qunCesIOl5horrrrs'uirrrundittg ritjr'l a. B. c D.' E'' A.E'.."..,...aucAAAcccce,...,,,,.uuucccAtAGc.,,3' B. s',,....",,TAc?Tyscccc.......,.tAAcccTTTcGc...3' c.s'....",,,,cccryr6ccAAA,,,......fccccr rcA"...3' B. g'....,,..,cccu{,IUGGGAAA..,,,..,,ccccuuucau,..t' r. s'...,,.,,^eccGGsrr"cAT"^.","...cccrrrGGgaaa.,,3'
Csrrect Arslrer i :. 5' ..... CaCT|T$GGAAA.... .. riCCtititiTTfC,AT...-?', e, cur Marks 1 orit of i

4l ilcrrt nran_rLllrrlll{}soill*s ilre llrele ir a }urman ferti}iz-*degg? aB. c D. E' a .2 2 . B, ?3, c.a l ' D,46. .4 a .
CoiJe(* Ar:gw*r l D.46. Y.our Marks : tcut0fi 5t i4idn ct ihe lbllosiirg iietemn!s are tnie?

r. .\gd>acterirm iujccts Ti plasnid i*1ri plant cells up*n infbclion, ll R:trtrviru-< is uscd iri human gene drarap,l because il can detiver gtrro into certfiifi t-yperjolhrimlrn cu:l|s. lii i..zliplasr::id:crinbi:u*:dftircl*nin{nndexpressi:rgihreigngenesin,':i:r:rli. l\ . 3kleftrrlha:-lejilhn:.{c ar!' vinrsr.s tlrul ca}} jnf!.ci bactr'da A I C DE. -t? a. r and Er onty. g. :. tt and tI! orly c.1 ttl.*d Iv only. D. tI, III asd Iv of,ty" E, r, tr,ttr and rv
Cgrrecf Ar-.syer Ycur l4!irks E. l,:i.IlI and lV

ouf sf :

6i I l)*r+g ihe Stuthernblot airalv-ris, can +*e irrcrease specrficily proce:;s horv the andaccuracy rrfthe detectir.rr
a BC D. C. a. 8y dsra$ing 3. 3y addia$ C, ay rcduaing D. Ey increff$is$ E. By ircrssing ttr *ef,perftture. morc bt{A irto the g1. bsffe.. Dr$re prcbq atrd ls&dlng the slt csnc*tratlon htbridimtion nf homslsgy

In the hytridi:atlon tlile"

the p$be th degru

ot the prob'e ta the ta.get. the cf

Coarcct Answr i vsur Flerk i ll cut +f :.

cf tne irobe t0 in

r) \'!iiny geneticaiSv-mo<iiiieil cr*pr *ill he *nrering1helrurnan l'*nd fo*d chain in lhr"y*ari to rorne \Vhjch of i:he fbil*wing is/are likelv inpirta*1 1{1 ensrlre thut Frclicallv nroililledfo*d is safeli:r huma*i:onsurr:piion'l

@',? !

I of4

IVLE A$sessment

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A. . A, f;ach g*netirslly

moditied tood *rop i$ ffireencd for its ffiistanre tc ailtibiotics. E. ', B. Earh genetically moditid food crsp is sresed fsa its diffitr rsistance. c' 'l C. Each genetically modified food crop i$ srnd fo. toxacltr. D , i E .F e n d C strty. E .:E .A ,i l a trd c.
Ct{ect Answer : :. Each gereircallv modifred fsd cros:s scrned fo. toxjcitv, Ycur fnlarL : lautofl

8) I hitvs tNLl {li:r!Fordet to ileieimine the exs.ct genetic luahe-up, I crossed tlrenr and set 4 cll springs. lVhich otlthe fbll+rung is liktlv ronectli a^ if the greenrwhit* ratio is 2;& th Sr*n pare*t i$ frcbabty pure br*d {homo:}gote} and the white * hyb.lrl thetefsr?gote} a. '. B. if t pet 3 gr#fi .nd 1 whitor the parents wcre probalrly hoth hyb.ids {heterozyg6t#}. C' - C' if I get 3 Ereen and I white' the gren is prstoab,ly pur*bred tb*morygote) a*d the white fr hybrtd a {heterozygcte}, D. .:::n" if they were all grcer, hoth parent dogs were probalrly pure bred {homo:ygats}, E, r, 6. Nst enrugh irfsrmation to determine,
Coarect Ana,urEr D. if lhey *erc ali grefi. v, o$r f'larkc lsutofl both paront dogs were probobly i?uiE bred (hon-.o:vqotesl

A'

!'N l Rs arel a. :, A. vari*ble numlre. of tandem amiils acid repeatr ihat are hlghtt heterogseous. B. " B, variable fiumhe$ af tandrm repffits that vrdes {hyperpolymorphic} in the pfputatior, c. i C. Bariable numb*rs of ta**em nscftrtide$ ttlat are tran*ribed feFstddly and varis in tire Fofulation, D' ' D" vnriable number$ of ta,ldem .epeats that are highty unitorm in the pofllatioil, E. E, variable numfer$ of tandem repeats of lmino arid .eseets that a{e unifcrm in the popul|tlon.
CGi reci Aesvief va.isble nunile6 \jour l4rkg l lout0fi

of tanden

ppqsls that varies (hyFe.polymapiic)

in the FgDulat;cn.

1ol i r1 i L o e .smrl th t:rhadar *r gr eoessiveldiseaseca*sedhvn$uta1i 0n$nl ]0ti no1 can irfli. What is rhe risl of Mr l.*e 's da*ghter? t. ghe will {tefinitly be a rarrier but will drt be $ick. B. '.' F. She witl Set the diffie inherited frcff hr graf,dtroiher, C. C. She may be a csrrietr fo. the disffi {abaut SO!/ohanqe}. D. s. She *ilt nft be a sarrier. A. E' E. Hct enough information io determine.
Coirect AnsBer r \. Sie vrill detinitel_v be a sfder hlit will nc{: be si{k.

vsur Merk! loGtofi

1r) GeneT is madeup of3 exons and 2 introns with their length indicatedbelow, exon 1: 267 nucleotides intron l: 1002nucleotides exon2: 603 nucleotides intron2: 3000nucleotides exon3: 126nucletodes Assumingthat there is only one start codon at the beginingofexon I and one stop codon at the end ofexon 3, what is the total length ofthe protein encodedby gene T?
a B' CD' E. t, i33 aHiso trids, B. 313 amitro trid$. C.33:. atriHa aids. acids" acids {o:recl .Arlwer 'lC ll1 dniro acidj. 'four M3rks : | 1 out f :.

D.936.nlno , 1333 amlls

1:) l\'iich A BC D. E.

ol' the lbllcwirrq A. The .spective B, The rpective C. Th rs'trtlve B. The rspalive E. Tn spectiv*

*orrertly

ilescribes

a. cldN lip? ot the sugar ltrk$ oxyg{n mclulG molsql* iloltrFles mclsul9 froltrules of the s$Ear llcks caygen moiecuies

3' and 4" 6r.ban 2' and 3' r*rbon 1'asd 3" carbsr

atom poFitions at*m afos atos Fqsl$ons Fositions pogitisng po$itisns

of the suGar Ircks orygen of the suga. lack$ axygen of ih sugar trcls oxfgn

Z' rnd 4' ffirbon 3' ard

5' carbon alom

of the $ugar lacks oxygen ato.r psitions

Cs.re{t Aflswer I ). The rspctlv* Ycur MarNi : 1 crit of i

?'tsrd l'i?rbot

13) l1'ha! are the advan'{*ges ot'edihle vaiJcineshave $ver traditinnal v;:ccines? I iher.. are *ruch morrr **'ectivr: ihan lraditio*al r,accines. ii, l.her erg e,asierto;*dlrrifti-(tei. l j t. Srlnr.i cl th{rlr ar* suiiable lirr hrng tr:rm storage. li:. -fhei crn b+ harrcst*d in l3rgc quarlut!. A. B. C D. E. a. I afid II onlf_ B. I, II afld III anly. C. r, Xil arld Iv oFty, D, l!, tlt ax: Iv *nly e. r. fi, IIt asd w.

2 of4

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Coirect Answer i .i. ll, l!: anC lV only Y.our l4erks I loutsf:

1q) Vji:icl of the filllo:lirg III. Ill. Iv. \/ A. B. r uee eilhef iiFLPs, VN ! Rs or S iRs?

Fensi! $aaence. Archdelo$y, Artisetse tqhnol0gy Prbliri h*ilth. A" t and !r onty" B. t aod XV $*ly.

A I

c. r, rr andrrr onlv" D . r , r t a n d l v on ty E. ':r E. r. ur rrr and ry.

Corect Anslver D. l. 1l and lV 6nly Your plarks : rcutofl

1s)
$Jhi.r af the follswing sratsfients i: tril iegardiilg tie liEt geie therapy trcatment? l. The t:.it attempts at Cene thep9 bCan in 1990, lI. :t was pirfrrmed ilrench Arlijeffiotr'$ gisup, lII, :he discfder is krov{n a3 Cysric Fibrosij, 'y j\./ lt ri,lolved iorreci]lljg a defe{( SCIi) qene. tA/ Y A. a1 -,6 a. ! and iI onlv" B. r, It ind III arty,

c
D-

c. t, rr and rv only.
D, II, III aild IV anly,

E. , E.r. r!, trr afd rs.

Coirecl i,riswr I A. I Bn.::II only. Your !l3rk5 0cutofi

!6}qSsUm.hatacjrc!|rp|asmid!5]'6c8baspeirsinlengthandha5rStri(tjcn9itestthenucletidep0sitj0n54|cand12c.3,Gi\,etheeKF :Egnerts fcllorling rc6Fle'.e rliggstion. I 4!3 lir, Ii- 9C0 bp. Irt. 1_?40 hr?. :.,;. 15i13 3p. a. a. c. D. E' a.: orly,

B. rr cnty_ C. I aFd tr o*$. D. I, II and ItI : E, t, rr, rn *sly,

and Iv ontY. Coirmt Answef i g.1i 0nly" -!'3ur Marks

loutsfi

17) -"+;4s fluores(ert labelled-ddN]lis, asi:oaated i)NA striuencirg ruiis or tl:e foll$Binq;

i 3tandard 4itiiPs. iI 3irer II:, -:aq DFJApo:yfler4se lV ad eiatrcpllc.si! A. B. A, t and ttl onty,

E. I and Iy qnly.

c D. E.

c, r. rr and rtr only. D. ! ttand Iv only, E. r. tr, ttr and rv.

CoireCa Arswer r
!, II anC Iv onlv. Y-our f'larks i loutofi

../

'*,

<et-*-aruses sre it$eful ir ggile ih+rary iiecarrsel i- :t i deiiv*r a gelre trto specific cli tyFS. Il- :t.an he:t lo ir:tegrate a ijne ir*c ihe ftji's geitome. lli. :t car acti.Jatei*plicatlon af the gen4 in th ceii, iY. !t sgntairi in aetive virsl Frcrnoter thai can Crive trnescription of tii* iJerie ;t: the (eii lBC D. E. t. I and tI cnty" B. II a*d Iv only" C. t, ll and IrI anly, D, r, u and w onty, r E, r. II, r$ aRd rv. Core{t Aeswer : \, I and II oily. loutof!

Your Marki 19)

i?-lici cf the foll$rviiE sre nrqumelits ihat ere agsinst patentins gene er Sene pJadu*? L teeting enc0uragls secrcv. II- %teFting incress rffit that v{iil i!ts$$ed dawe !s coilsutrer$. !I1. Paterting encouiaqes innsvetion anC !nvestmert, IV. Coii a5ssiatsl wiih il-cin! patrf,led^rss6r*t ilata (un irnpeie Leitairi r$e6rc*i .jevelcp.nent

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A. B.

A. :I only. F. I and ll anly, C. 1I and XII only. P. It and IV only, g. I: 11 and IV cf,ly.
Corrcct AFswer Ycur Marki D. II ard lV oriy. loltofi

c.
D, E.

2s)
Whicr sf th fallswitg procedures niust j.vslse efibryenjc mause $te!r cells? I- GeieFtion of a krock-out lL Gene therapy. l1I. F.errod!ctive clonins, lV. Tne*peul:( clo0itg. A. B. C' D. E. ': &. I snd tII 0nly,

r B. t! and trv onty. ': C, f lsd lll only. . D" I, III and !v only,

. r, rr, ffis and rv, a:oarect An$wer i a:. I bn.i IV only.

Y0ur Merks 0 cut of :.

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