JUSTIN FRANKLIN SHAFFER

58 Abbey Road, Chapel Hill, NC 27516 (724) 301-2712 shafferj@unc.edu http://shafferj.web.unc.edu/ EDUCATION______________________________________________________________ ___ Ph.D. in Bioengineering June 2010 University of Washington, Seattle, WA Spent July 2007 to June 2010 at the University of California - Davis, Davis, CA B.S. in Chemical Engineering, Bioprocessing Option, with Honors Pennsylvania State University, University Park, PA May 2005

AWARDS, HONORS, AND FELLOWSHIPS_________________________________________ Postdoctoral Scholar Award for Excellence in Mentoring Undergraduates, UNC 2011 Postdoctoral Scholar Award for Excellence in Service, UNC 2011 SPIRE Postdoctoral Fellowship, UNC (NIGMS K12GM000678) 2010 - 2013 Deans Circle Mentorship Award, UC Davis College of Biological Sciences 2009 Young Investigator Prize, European Muscle Conference, Lille, France 2009 Biophysical Society Annual Meeting Student Travel Award, Boston, MA 2009 Graduate Student Travel Award, University of Washington Graduate School 2008 National Science Foundation Graduate Research Fellowship 2007 - 2010 NSF Graduate Research Fellowship Program, Honorable Mention 2005, 2006 First place, MindBend Symposium (Undergraduate Research), Penn State 2005 First place, Undergraduate Research Symposium, Penn State 2005

TEACHING EXPERIENCE_______________________________________________________ Principles of Biology Spring 2013 Department of Biology, University of North Carolina at Chapel Hill Visiting Lecturer Taught ~400 undergraduate majors and non-majors students using student-centered learning principles and active learning exercises Developed and implemented original lectures, activities, worksheets, and exams Supervised and mentored graduate student teaching assistants and undergraduate supplemental instructors Investigated student self-efficacy with educational research tools Designer Proteins and Society Fall 2012 Department of Biology, North Carolina A&T State University Adjunct Professor Developed a novel course that explored the design process behind the production of recombinant proteins and their link to medicine, industry, and agriculture Prepared original lectures, exams, assessments, and group activities, as well as a final design project that required students to propose and justify a process to clone, express, purify and assess a recombinant protein Implemented a 5-week hands-on laboratory module based on the Bio-rad Biotechnology Explorer Protein Expression and Purification Series Taught this course to 16 undergraduate and graduate biology students

Shaffer 2 Biological Sciences (Introduction to Biology for Non-Majors) Spring 2012 Department of Biology, North Carolina A&T State University Adjunct Professor Taught 71 undergraduate non-major students in an active classroom where I used original lectures, activities, assessments and real-world examples and case studies Achieved an average learning gain of 23% using a pre-test and post-test assessment Supervised and mentored three graduate student TAs that each taught laboratory sections of 24 students that explored lecture material in more detail Biology Research Mentor Training Program Spring and Fall 2012 University of North Carolina, Chapel Hill Facilitator Facilitated and coordinated six-week workshops with ~20 participants (graduate students and postdocs) that focused on effective undergraduate mentoring practices Comparative Physiology Spring 2011 Department of Biology, University of North Carolina, Chapel Hill Guest Lecturer Presented two original guest lectures to 70 undergraduate students: invertebrate excretion; and animal movement and muscle physiology The Physics of Life Fall 2010 Department of Biology, North Carolina Central University Guest Lecturer Presented original guest lecture to 10 students on muscle mechanics and motor proteins Introduction to Biology: Biodiversity and the Tree of Life Winter 2010 Department of Biology, University of California, Davis Laboratory Assistant (with Tom Goliber, PhD) Worked with 24 undergraduate students on weekly in-lab assignments and answered student questions Major Discoveries in Muscle: Myofilament Proteins Fall 2009 Department of Neurobiology, Physiology, and Behavior, University of California, Davis Instructor Created novel course to examine historical advances in muscle research Developed original course syllabus and lectures Oversaw student presentations and led group discussions of 12 graduate students Bioengineering Design Principles Spring 2007 Department of Bioengineering, University of Washington Teaching Assistant (with Shahram Vaezy, PhD) Advised 60 undergraduate students on research and design projects Created and presented original lectures Graded and provided feedback for research project reports, digital stethoscope projects, and oral presentations

Shaffer 3 RESEARCH EXPERIENCE______________________________________________________ SPIRE Postdoctoral Research 2010 - present SPIRE - Seeding Postdoctoral Innovators in Research and Education Department of Biology, University of North Carolina at Chapel Hill Adviser: William Kier, PhD, Chair and Professor of Biology Investigated the diversity of myosin motor proteins in cephalopod muscles using molecular cloning and DNA sequencing methods Initiated a Dictyostelium discoidium culture system in order to express recombinant squid myosin proteins Ph.D. Doctoral Research 2006 - 2010 Department of Bioengineering, University of Washington Department of Neurobiology, Physiology, and Behavior, University of California, Davis Advisor: Samantha Harris, PhD, Assistant Professor of Neurobiology, Physiology, and Behavior Investigated molecular mechanisms of the role of cardiac myosin binding protein-C (cMyBP-C) in the regulation of striated muscle contraction using biochemical and biophysical assays Created recombinant wild-type, mutant, and chimeric proteins using molecular cloning, site-directed mutagenesis, and protein expression and purification techniques Undergraduate Honors Research 2003 - 2005 Department of Chemical Engineering, Penn State University Advisor: Andrew Zydney, PhD, Chair and Professor of Chemical Engineering Investigated adverse effects of bleach reprocessing on hemodialysis performance Engineering Internship Summer 2005 Bristol-Myers Squibb Company, Syracuse, NY Optimized a purification system for large-scale production of anti-arthritis drug Scientist Internship Summer 2004 Kimberly-Clark Corporation - Safeskin R&D, Roswell, GA Optimized Tactylon synthetic surgical glove formulation by addition of co-solvents STUDENT MENTORING EXPERIENCE____________________________________________ North Carolina A&T State University Deionta Sutton, Animal Sciences Freshman 2012 o Honors option student in Introductory Biology course who completed a research paper and presentation on the tonic activity in lemon sharks University of North Carolina at Chapel Hill Carlos de Castro, Biology Senior 2011 - 2012 o Best poster award winner at 2012 UNC Celebration of Undergraduate Research Alli Sarfati, Biology Senior, HHMI-Future Teachers Program 2011 - 2012 o Presented a poster at the 2012 SICB Annual Meeting and created an online learning module for high school students and teachers about the relationships between protein sequence, structure, and function Alex Holland, Biology Senior 2011 o Investigated myosin expression in squid muscles

Shaffer 4 University of California, Davis Camelia Dumitras, Neurobiology, Physiology, and Behavior Senior 2008 - 2010 o Created and assessed recombinant full-length human cMyBP-C Peony Wong, Biochemistry Senior 2008 - 2009 o Created and assessed recombinant human-mouse chimeric cMyBP-C proteins and was second author on a peer-reviewed publication University of Washington Jordan Kuester, Bioengineering Junior o Assisted with in vitro motility experiments

2006 - 2007

EDUCATIONAL OUTREACH EXPERIENCE________________________________________ UNC Office of Graduate Education, Science Outreach Program 2010 - present Volunteer Developed a summer high school research experience program (UNC SHARP) that brought high school students to UNC to participate in scientific research Developed and presented workshops to UNC PREP post-baccalaureate students on basic laboratory techniques, sterile technique, and BLAST web tools Worked in a group to develop a learning module for NC DNA Day on Demand, an online video that can be used in school classrooms (http://ncdnaday.org/ondemand/) UC Davis Biomedical Engineering Student Association (BESA) 2009 - 2010 Educational Outreach Chair Interacted with Sacramento area elementary, middle, and high school teachers to schedule and execute 13 visits reaching approximately 800 students Directed 30+ biomedical engineering graduate and undergraduate students to develop original biomedical engineering content (medical imaging, biomechanics, and cellular and tissue engineering) including a presentation and hands-on activities University of Washington Biomedical Engineering Society (BMES) 2006 - 2007 Educational Outreach Chair Interacted with Seattle area middle, and high school teachers to schedule and execute 10 visits reaching approximately 800 students Directed ~15 bioengineering graduate and undergraduate students to develop original bioengineering content including a presentation and hands-on activities with biomaterials and other medical devices PROFESSIONAL DEVELOPMENT________________________________________________ Teaching and Mentoring Workshops / Courses Biology Research Mentor Training Program (Facilitator), UNC SPIRE Seminar on College Teaching, UNC SPIRE Distinguished Scholar Seminar (Co-organizer), UNC College Science Teaching, UNC Biology Advanced Communication Skills, UNC Effective College Teaching, UNC Scientific Teaching, UC Davis Thinking Before you Teach, UC Davis Entering Mentoring Program, UC Davis 2012 2011 2011 2011 2011 2010, 2012 2009 2009 2008 - 2009

Shaffer 5 Professional Societies Society for the Advancement of Biology Education Research, Member Association for Biology Laboratory Education, Member Society for Integrative and Comparative Biology, Member European Society for Muscle Research, Member Biophysical Society, Student Member UC Davis Biomedical Engineering Student Association (BESA) o Co-Community Service/Outreach Chair UW chapter of the Biomedical Engineering Society (BMES) o Outreach Chair

2012 - present 2012 - present 2010 - present 2008 - 2010 2006 - 2010 2009 - 2010 2009 - 2010 2005 - 2010 2006 - 2007

PUBLICATIONS_______________________________________________________________
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Shaffer JF, Kier WM: Muscular tissues of the squid Doryteuthis pealeii express identical myosin heavy chain isoforms: An alternative mechanism for tuning contractile speed, J Exp Biol, 215: 239-246, 2012. Kensler RW, Shaffer JF, Harris SP: Binding of the N-terminal fragment C0-C2 of cardiac MyBPC to cardiac F-actin, J Struct Biol, 174: 44-51, 2011. Shaffer JF, Gillis TE: Evolution of the regulatory control in vertebrate muscle: The roles of troponin I and myosin binding protein-C, Physiol Genomics, 42: 406-419, 2010. Jia W, Shaffer JF, Leary JA, Harris SP: Identification of novel PKA phosphorylation sites in the M-domain of murine and human myosin binding protein-C, J Proteome Res, 9: 1843-1853, 2010. Shaffer JF, Wong P, Bezold KL, Harris SP: Functional differences between the N-terminal domains of mouse and human cardiac myosin binding protein-C, J Biotechnology and Biomedicine, 2010: Article ID 789798, 2010. Shaffer JF, Harris SP: Species-specific differences in the Pro-Ala rich region of cardiac myosin binding protein-C, J Mus Res Cell Motil, 30: 303-306, 2009. Shaffer JF, Kensler RW, Harris SP: The Myosin binding protein-C motif binds actin in a phosphorylation sensitive manner, J Biol Chem, 284: 12318-12327, 2009. Shaffer JF, Razumova MV, Tu AY, Regnier M, Harris SP: Myosin S2 is not required for effects of MyBP-C on motility, FEBS Letters, 581: 1501-1504, 2007. Razumova MV, Shaffer JF, Tu A, Flint GV, Regnier M, Harris SP: Effects of the N-terminal domains of myosin binding protein-C in an in vitro motility assay: Evidence for long-lived crossbridges, J Biol Chem, 281: 35846-35854, 2006. Shaffer JF, Zydney AL: Phosphate clearance for bleach reprocessed hemodialyzers: Effects of electrostatic interactions, ASAIO J, 51: 748-753, 2005. polysulfone

Shaffer 6 RESEARCH SEMINARS________________________________________________________ Diversity (or lack thereof) of myosin motors in cephalopod muscles, Department of Biology, University of North Carolina Chapel Hill, Chapel Hill, NC, 23 September 2011. Diversity of muscle myosin motors in cephalopods, Department of Biology, Fayetteville State University, Fayetteville, NC, 18 March 2011. Diversity of muscle myosin motors in cephalopods, Department of Biology, North Carolina Central University, Durham, NC, 16 February 2011. Diversity of muscle myosin motors in cephalopods, Department of Biology, University of North Carolina Pembroke, Pembroke, NC, 15 February 2011. Diversity of muscle myosin motors in cephalopods, Department of Biology, Johnson C. Smith University, Charlotte, NC, 10 February 2011. Diversity of muscle myosin motors in cephalopods, Department of Biology, North Carolina A&T State University, Greensboro, NC, 9 February 2011. Interactions between cardiac myosin binding protein-C and actin contribute to the regulation of muscle contraction, Lab of Bill Kier, Department of Biology, University of North Carolina, Chapel Hill, NC, 23 April 2010. Interactions between cardiac myosin binding protein-C and actin contribute to the regulation of muscle contraction, Lab of Todd Gillis, Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada, 18 December 2009. Interactions between cardiac myosin binding protein-C and actin contribute to the regulation of muscle contraction, Lab of Henk Granzier, Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, 12 November 2009. Interactions between cardiac myosin binding protein-C and actin contribute to the regulation of muscle contraction, Lab of Wolfgang Linke, Physiology and Biophysics Unit, University of Muenster, Muenster, Germany, 17 September 2009. ORAL PRESENTATIONS_______________________________________________________
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Shaffer JF, Kier WM: Muscular tissues of the squid Doryteuthis pealei express identical myosin heavy chain isoforms, Society of Integrative and Comparative Biology Annual Meeting, Charleston, SC, January 2012. Shaffer JF, Harris SP: Identification of amino acid residues in the myosin binding protein-C motif important for actin binding, Biophysical Society 54th Annual Meeting, San Francisco, CA, February 2010. Shaffer JF, Wong P, Bezold KL, Harris SP: Comparative effects of the N-terminal domains of human and murine cardiac myosin binding protein-C, European Muscle Conference, Lille, France, September 2009.

Shaffer 7 Shaffer JF, Kensler RW, Harris SP: The N-terminus of cardiac myosin binding protein-C contains multiple binding sites for F-actin, Biophysical Society 53rd Annual Meeting, Boston, MA, March 2009. SELECTED POSTER PRESENTATIONS___________________________________________
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Shaffer JF: Insulin: The First Recombinant Drug Development and Assessment of a Novel Biotechnology Case Study, Bridging the Gap NC, Raleigh, NC, October 2012. Sarfati AL, Shaffer JF, Kier WM, Coble JS: Using research focused learning modules for outreach to high school science classrooms, Society of Integrative and Comparative Biology Annual Meeting, Charleston, SC, January 2012. Shaffer JF, Kier WM: Muscular tissues of the squid Doryteuthis pealei express identical myosin heavy chain isoforms, Annual IRACDA Conference, Houston, TX, June 2011 Shaffer JF, Wong P, Bezold KL, Harris SP: Comparative effects of the Pro-Ala rich regions of human and murine myosin binding protein-C, Biophysical Society 54th Annual Meeting, San Francisco, CA, February 2010. Shaffer JF, Jia W, Leary JA, Harris SP: PKA phosphorylates serine 307 of murine cardiac myosin binding protein-C in vitro, Biophysical Society 53rd Annual Meeting, Boston, MA, March 2009. Shaffer JF, Harris SP: Interactions of the N-terminus of cardiac myosin binding protein-C with Factin, European Muscle Conference, Oxford, England, September 2008. Shaffer JF, Razumova MV, Tu AY, Regnier M, Harris SP: The cardiac myosin binding protein-C motif and C1 domain activate actomyosin motility independent of Ca2+, Biophysical Society 52nd Annual Meeting, Long Beach, CA, February 2008. Shaffer JF, Razumova MV, Regnier M, Harris SP: The C1C2 domains of myosin binding protein C inhibit acto-S1 movement in an in vitro motility assay, Biophysical Society 51st Annual Meeting, Baltimore, MD, March 2007.