F2E-(12)-5

DETECTING E.COL1-0157 WITH FIBER-OPTIC BIOSENSOR

TuShin Chan, Chien Chou, Chiemyuan Han, HsiehTing Wu, a d Zhi-Ren You n htitute of Radiologid Sciences, ~ational Yang-Mug university 155 Sec. 2 Li-Long St Pei-Tou, Taipei, 112, Taiwan, ROC Phone 886-2-28267061, Fax 886-2-28251310, &Mail g39020022@ym.edu.tw
Abstract -We develop a fiber-optic biosensor system for rapid identification o E.coli-0157 and f calculate the kinetics ofIgG.

INTRODUCTION
Escherichia coli is a kind of bacteria, and it will cause the enterohemorrhagic E.coli infection. Though the types of E.coli that cause this kind of infection are many, most of them have no other dangerous complications. But there is one type E.coli-0157: H7, will not only cause the

enterohemorrhagic E.coli infection, but also bring about the dangerous hemolytic uremic syndrome that might cause human death. So, it is important to build up a system for rapid identification of Escherichia coli in clinical.

METHODS AND MATERIALS

We use a 488nm argon guide laser and guide into an objective to match the numerical aperture of the fiber and let the light propagate by total internal reflection. A 5cm length region of fiber had been cladding by ethyl acetate and the <antibody-antigen-FITC labeled antibody> sandwich structure were immobilized on this region and will be excited by the evanescent wave, then release 520nm fluorescence. The fluorescence signal will be detected by the PMT. PMT will send the signal to the lock-in amplifier and analyze them.

RESULTS A N D DISCUSSION
Here we prepare four different kinds of E.coli subtypes, and the results show that only E.coli-0157: H7 will react with the fiber-optic biosensor and cause the signal intensity change. That means our system has the specific binding affinity with the target sample. Except the quantitative and qualitative analysis, we also calculate the kinetics. We have four different concentrations of IgG samples, and the results show that the and 4.4 x lo" s-'.

kssand 4 , .are 6 x

106 M I S - '

REFERENCES
[ I ] Padhye, N.V. and M. P. Doyle (1992). Escherichis coli 0157:H7:epidemiology,pathogenesis, and
methods far detection infoad. J. Food Prot. 55555-565.

[2] Bluestein, B., Walczak, I. M., and Chen, S. Y. (1990). Fiber optic evanescent wave immunosensors
for medical diagnostics. Trends Biotechnol. 8 161-7.

[3] D. Ivnitski, T. Wolf, B. Solomon, G. Fleminger, I. Rishpon. An amperometric biosensor for

f real-time analysis o molecular recognition. Bioelectrochemistry and Bioenergeties (1998) 45

27-32
0-7803-7766-410311617.00 02003 IEEE

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