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Tissue culture is the growth of tissues and/or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, while the more specific term plant tissue culture is used for plants. Tissue culture produces clones, in which all product cells have the same genotype (unless affected by mutation during culture)
Historical usage
In 1885 Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog nerve cell processes in a medium of clotted lymph.
Modern usage
In modern usage, "tissue culture" generally refers to the growth of eukaryotic cells in vitro. It is often used interchangeably with cell culture to specifically describe the in vitro culturing of sperm donor cells. However, "tissue culture" can also be used to refer to the culturing of tissue pieces, i.e. explant culture or whole organs, i.e. organ culture. It is a tool for the study of animal cell biology in vitro model of cell growth to allow a highly selective environment which is easily manipulated (used to optimize cell signaling pathways). In 1920 germen scientist Harbland is considered as the father of tissue culture.
Tissue culture, both plant and animal has several critical requirements
1. Appropriate tissue (some tissues culture better than others) 2. A suitable growth medium containing energy sources and inorganic salts to supply cell growth needs. This can be liquid or semisolid 3. Aseptic (sterile) conditions, as microorganisms grow much more quickly than plant and animal tissue and can over run a culture 4. Growth regulators - in plants, both auxins & cytokinins. In animals, this is not as well defined and the growth substances are provided in serum from the cell types of interest 5. Frequent sub culturing to ensure adequate nutrition and to avoid the buildup of waste metabolites
Classification
Tissue culture may be of two types based on medium. 1. Plant tissue culture 2. Animal tissue culture
3. Establishment of the explant in a culture medium. medium sustains the plant cells and encourages division. It can be solid or liquid 4. Each plant species (and sometimes the variety within a species) has particular medium requirements that must be established by trial and error 5. Multiplication- The explant gives rise to a callus (a mass of loosely arranged cells) which is
The cell
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times
7. Root formation - The shoots are transferred to a growth medium with relatively higher auxin: cytokinin ratios 8. The rooted shoots are potted up (deflasked) and hardened off by gradually decreasing the humidity 9. This is necessary as many young tissue culture plants have no waxy cuticle to prevent water loss
g) As a tissue for transformation, followed by either short-term testing of genetic constructs or regeneration of transgenic plants. h) Certain techniques such as meristem tip culture can be used to produce clean plant material from virused stock, such as potatoes and many species of soft fruit. i) micropropagation using meristem and shoot culture to produce large numbers of identical individuals
Bibliography
1. Dodds, J.H., Roberts, L.W., 1995, Experiments in Plant Tissue Culture, 3rd ed., Cambridge University Press 2. Hartmann, H., Kester, D., et.al., 1997, Plant Propagation, 6th ed., Prentice Hall International 3. http://www.une.edu.au/agronomy/AgSrHortTCinfo.html 4. http://aggie-horticulture.tamu.edu /tisscult/pltissue/pltissue.html 5. http://www.liv.ac.uk/~sd21/tisscult/what.htm 6. http://user.school.net.th/~anoparp/bptc1.htm