Code No: RR322302 Set No.

1
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. What is the role of microscopes in microscopy? [16]

2. What do you understand by confocal microscopy? [16]

3. Differentiate between ?

(a) Reducing and non-reducing sugars

(b) Mono, di and poly saccharides [8+8]

4. What is meant by Isoelectric point of a protein? [16]

5. Explain, ”Western blotting is an extension of protein Electrophoresis”. [16]

6. What is meant by DNA polymorphisms? How are they detected? [16]

7. What is meant by Immunohystocompatibility? [16]

8. Discuss the kinetics of substrate uptake in cell culture. [16]

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Code No: RR322302 Set No. 2
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. How is the dark field created in the Dark Field Microscopes? [16]

2. What is freeze fracture or freeze Etching technique? [16]

3. What are Nucleotides? At what wavelength can they be absorbed and why? [16]

4. List the difference between 1D and 2D Electrophoresis. [16]

5. Compare the different filters available for doing hybridization experiments. [16]

6. How is DNA fingerprinting done? What are its advantages? [16]

7. Explain ELISA technique in detail. [16]

8. What is meant by flow injection analysis? [16]

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Code No: RR322302 Set No. 3
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe a Dark Field Microscope. [16]

2. Describe the construction of a SEM. [16]

3. What are Nucleotides? At what wavelength can they be absorbed and why? [16]

4. Describe how polyacrylamide gels get polymerized. [16]

5. What is GLC? Explain it in detail. [16]

6. How is DNA fingerprinting done? What are its advantages? [16]

7. Discuss about Flow Cytometry. [16]

8. Explain the terms -

(a) Dissolved Oxygen (DO)

(b) Dissolved Carbon dioxide (DCO2 )
(c) Oxygen transfer rate (OTR)
(d) Oxygen uptake rate (OUR) [4+4+4+4]

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Code No: RR322302 Set No. 4
III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007
ANALYTICAL TECHNIQUES IN BIOTECHNOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Describe the path of light in a Bright Field Microscope. [16]

2. Describe the construction of a TEM. [16]

3. Which are the common lipids present in a cell? How are they analyzed? [16]

4. Discuss the roles of acrylamide and TEMED in preparation of Electrophoresis gels.

- [16]

5. What are ” mobile’ and ” stationary”phases in any Chromatography? [16]

6. What is the principle behind using 2?, 3?, dideoxynucleoside triphosphates in DNA
sequencing? [16]

7. What is the principle involved behind RIA? [16]

8. Explain the terms -

(a) Dissolved Oxygen (DO)

(b) Dissolved Carbon dioxide (DCO2 )
(c) Oxygen transfer rate (OTR)
(d) Oxygen uptake rate (OUR) [4+4+4+4]

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