Code No: R050212305 Set No.

1
II B.Tech I Semester Regular Examinations, November 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. Discuss the following and how these processes proved to be a death blow to spon-
taneous generation theory:

(a) Tyndalization
(b) Pasteurization. [8+8]

2. Discuss the general characters of domain Bacteria, with suitable examples. [16]

3. Explain why some single stranded RNA viruses have +ve strands while others have
-ve strands. [16]

4. Discuss the steps in the process of penetration of bacteriophage into the host cell.
[16]

5. Define assay. Explain various types of viral assays. [16]

6. What are nutrients and on what basis are they divided into macroelements and
micronutrients or trace elements? Describe some ways in which macroelements
and micronutrients are used by an organism. [16]

7. Define water activity and briefly discuss how it can be determined. Why is it
diffcult for microorganisms to grow at low water activity (aw) values. [16]

8. What are the various chemical agents that are used for control of microorganisms
growth. [16]

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Code No: R050212305 Set No. 2
II B.Tech I Semester Regular Examinations, November 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Discuss the discoveries of Redi, Needham, Spallanzani, and Ferdinand in disproving

the theory of spontaneous generation. [16]

2. Describe different methods of classification of Bacteria. [16]

3. Explain prions with examples. [16]

4. Explain the entry of viruses into host cells. [16]

5. Define assay. Explain various types of viral assays. [16]

6. Define and explain autotrophs, heterotrophs and mixotrophs. Discuss their nutri-
tional relationship and their carbon source? [16]

7. Explain the influence of environmental factors on growth of microorganisms. [16]

8. Briefly explain how the effectiveness of antimicrobial agents varies with population
size, population composition, concentration or intensity of the agent, treatment
duration, temperature and local environmental conditions. [16]

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Code No: R050212305 Set No. 3
II B.Tech I Semester Regular Examinations, November 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Discuss the role of Lazarro Spallanzani and Ferdinand Cohn in disproving the
doctrine of spontaneous generation of microorganisms. [16]

2. Discuss about the major characteristics used in classification of microorganisms.

[16]

3. Discuss about virus associated human cancers. [16]

4. Discuss general steps in viral replication. [16]

5. Define assay. Explain various types of viral assays. [16]

6. Write about the following:

(a) Types of culture media.

(b) Pure culture methods. [8+8]

7. Define water activity and briefly discuss how it can be determined. Why is it
diffcult for microorganisms to grow at low water activity (aw) values. [16]

8. What are the various chemical agents that are used for control of microorganisms
growth. [16]

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Code No: R050212305 Set No. 4
II B.Tech I Semester Regular Examinations, November 2007
MICROBIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. As a microbiologist what is your concern about public health. [16]

2. How PCR technique is useful in classifying bacteria. [16]

3. Write short notes on:

(a) Enveloped virus

(b) Icosahedral capsids. [8+8]

4. Discuss general steps in viral replication. [16]

5. How is a one step growth experiment carried out ? Summarize what occurs in each
phase of the resulting growth curve. Define latent period, eclipse period, rise period
and burst size. [16]

6. What are nutrients and explain the physiological functions of nutrients. [16]

7. Write short notes on:

(a) Acid-fast staining.

(b) Flagellar staining.
(c) Spore staining.
(d) Differential staining. [4+4+4+4]

8. Briefly explain how the effectiveness of antimicrobial agents varies with population
size, population composition, concentration or intensity of the agent, treatment
duration, temperature and local environmental conditions. [16]

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