*Points Awarded* *8.00* *Points Missed* *0.00* *Percentage* *100%* Correct Incorrect Ungraded 1.

We are running a phenobarbital level on Mary Garcia. She has a history of seizures and arrives unconscious at the ED. The instrument reported a result of 8.0 mg/dL. According to the manufacturer, the instrument's reportable range is 7-120; however, the most recent calibration verification performed in our lab used a low-end calibrator of 10 mg/dL with a result of 10.0 mg/dL. What result is reported for this patient? less than 10 mg/dL *Feedback: * The result must be reported as " < 10 mg/dL" because the calibration does not cover the result of 8 mg/dL. A calibration verification is testing or verifying what the manufacturer claims as the reprotable range. In this case - the machine is supposed to detect from 7 - 120 mg/dL, HOWEVER - YOUR LAB must verify this and use its own values for the lowest and highest end of the range. In this situation - your lab's lowest value was 10, so that is the lowest you can report. good job Points Earned: 1.0/1.0 *Correct Answer(s):* The result must be reported as " _<_ 10 mg/dL" because the calibration does not cover the result of 8 mg/dL. A calibration verification is testing or verifying what the manufacturer claims as the reprotable range. In this case - the machine is supposed to detect from 7 - 120 mg/dL, HOWEVER - YOUR LAB must verify this and use its own values for the lowest and highest end of the range. In this situation - your lab's lowest value was 10, so that is the lowest you can report.

Correct Incorrect Ungraded 2. We are running an amylase on a patient with a history of alcoholism and a suspected case of pancreatitis. The last amylase reported (6 hours ago) was 1680mg/dL. (hint it might be about this level or a little higher or lower now) Our reportable range on this analyzer is *5-500 mg/dL*. You will use saline as diluent - and the instrument requires a sample size of at least *500uL*. 1. What dilution should we make? (for example - state "1 to 20")

2. How do you go about this dilution? (Do not use more than 500uL of patient sample, explain exactly, like "use 10mL sample and 200 mL diluent") 3. Support your answer, explain your reasoning for your dilution. You only have 2 mL or sample - and you don't want to use it all up on this test-you might need it for another chemistry test - so do NOT answer that you will dilute it x2 and use 2mL of sample and 2mL of saline! (that is wasting sample and you do not need 4 mL on this machine!) 1 to 10, because its simpler. Add and mix patient sample (100uL) and saline diluent (900uL) because need at least 500uL. Then times the answer by 10. The reading should be about 168 mg/dL with the second dilution then all you have to do is times it by 10 (1680). Which is good because its simple and the second (168) reading is in the "reportable range on this analyzer 5-500 mg/dL." *Feedback: *X5 or X10 would be acceptable. I would choose X10.I would use 100uL patient sample and 900uL saline - mix well and place on instrument. Then multiply result by 10. (could also use 50uL and 450 uL)The new result should end up in the middle of the reportable range for example, I am expecting the result to be somewhere around 1000 2000 based on the prior result of 1680 (which means the result was 168 mg/dL on the instrument, then multiplied by 10 - so this new result should be about 100 - 200 mg/dL) great job Points Earned: 3.0/3.0 *Correct Answer(s):* 1 to 5 or 1 to 10 would be acceptable. I would choose 1 to 10. I would use 100uL patient sample and 900uL saline - mix well and place on instrument. Then multiply result by 10. The 1 to 10 dilution result should end up in the middle of the reportable range for example, I am expecting the result to be somewhere around 100 - 200 based on the prior result of 1680 (which means the result was 168 mg/dL on the instrument, then multiplied by 10).

Correct Incorrect Ungraded 3. A 1 to 2 serial dilution of serum is carried out to 5 tubes. What would the 5th tube's dilution factor be? A) 8 B) 16 C) 32 D) 64 Points Earned: 1.0/1.0 *Correct Answer(s):* C

Correct Incorrect Ungraded 4. A sample is tested for beta-hcg (hormone). The sample was diluted as follows- 100 uL sample added to 900 uL of diluent fluid - mixed well, then 10 uL of that dilution was added to 190 uL of diluent fluid. This final dilution was tested and the result was 120 miu/mL. What is the final test result? ________ miu/mL (do NOT need to

use scientific notation and you do not need to change anything with the units) *Feedback: *dilutions - 100 and 900 - (100 out of 1000 total is x10 dilution) that is diluted 10 and 190 (10 out of 200 total is x20) total dilution amount is 10 x 20 = 200Final result is 120 x 200 = 24,000 miu/mL Points Earned: 2.0/2.0 *Correct Answer(s):* 24,000

Correct Incorrect Ungraded 5. A serum AST is diluted 1 to 100 with a result of 89.0 U/L. What is the patient's actual AST result? A) 890.0 U/L B) 8.9 U.L C) 87.2 U/L D) 8900 U/L Points Earned: 1.0/1.0 *Correct Answer(s):* D