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Abstracts / Toxicology Letters 205S (2011) S60S179

S131

could be involved in the adverse lung effects of this indoor volatile compound. doi:10.1016/j.toxlet.2011.05.465

P1232 Circulating levels of p,p-DDE are predictive of the development of insulin resistance during a 5 year follow up L. Lind 1, , D. Lee 2 , D.R. Jacobs 3 , S. Salihovic 4 , B. vanBavel 4 , P.M. Lind 5
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Acute and Internal Medicine, Department of Medicine, Uppsala University, Uppsala, Sweden, 2 Department of Preventative Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea, 3 Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, USA, 4 Mtm Research Center, School of Science and Technology, rebro University, rebro, Sweden, 5 Occupational and Environmental Medicine, Uppsala University, Uppsala, Sweden Purpose: Persistent organic pollutants (POP) have been suggested to be linked both to obesity and insulin resistance. However, this has mainly been found in cross-sectional studies. We investigated prospectively the predictive power of POPs for incident insulin resistance. Methods: 1016 subjects aged 70 years were investigated in the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) study and reinvestigated 5 years later. Twenty-one different POPs including 16 PCBs, 3 pesticides, one dioxin and one brominated compound (BDE47) were analyzed using high resolution chromatography coupled to high resolution mass spectrometry (HRGC/HRMS). HOMA insulin resistance index was calculated in the investigations at both 70 and 75 years old. Results: During these 5 years, 51 subjects have developed insulin resistance (HOMA > 75 percentile at age 70). Excluding subjects with insulin resistance at age 70, among the evaluated POPs only p,p-DDE was a predictor of incident insulin resistance independently of BMI and lipids (OR 4.72 for 5th quintile vs 1st, 95%CI 1.4914.9, p = 0.008). Conclusion: Circulating concentration of the OC pesticide metabolite p,p-DDE was a predictor of future insulin resistance independently of obesity and hyperlipidemia. doi:10.1016/j.toxlet.2011.05.466

MetS. Methods: 1016 subjects aged 70 years were investigated cross-sectionally in the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) study. According to BMI/MetS-status, they were categorized as normal weight (BMI < 25 kg/m2 ) without MetS (NCEP-criteria, n = 319), normal weight with MetS (n = 19), overweight (BMI 2529.9 kg/m2 ) without MetS (n = 333), overweight with MetS (n = 94), obese (BMI 30 kg/m2 ) without MetS (n = 102), and obese with MetS (n = 118). 16 PCBs and 3 OC pesticides were analyzed by HRGC/HRMS. RESULTS: The sum of pesticide concentrations was positively associated with both BMI and the MetS independently of each other (p = 0.005 and p = 0.0001). The sum of less-chlorinated PCB concentrations (congeners 153) was positively associated with the MetS (p = 0.018), but no independent signicant association with BMI was seen. The sum of highly chlorinated PCB concentrations (congeners 157) was inversely associated with BMI (p = 0.0001), but no independent signicant association with the MetS was seen. Conclusion: Different PCBs were related to obesity and the MetS in different ways, while the investigated pesticides were related to both obesity and the MetS. doi:10.1016/j.toxlet.2011.05.467

P1234 Are histopathological endpoints useful for the evaluation of natural soils contaminated by metals and radionuclides? J.I. Lourenco 1, , A.C. Silva 1 , F.P. Carvalho 2 , J.M. Oliveira 2 , M.P. Malta 2 , S.A. Mendo 1 , F. Goncalves 1 , R.O. Pereira 1
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Department of Biology and Cesam, Universidade de Aveiro, Aveiro, Portugal, 2 Unidade De Protecco E Seguranca Radiolgica, Instituto Tecnolgico Nuclear, Sacavm, Portugal Purpose: The main objective of this study was to assess the sensitivity of histopathological endpoints, to be considered in the standard earthworms reproduction assay, performed to evaluate the ecotoxicological impact of soils contaminated with radioactive wastes. Methods: Eisenia andrei was exposed, for 56 days, to a contaminated soil from an abandoned uranium mine and to the natural reference soil LUFA 2.2. The organisms were sampled after 0, 1, 2, 7, 14 and 56 days of exposure, to assess metals bioaccumulation. The bioaccumulation of radionuclides, growth and the occurrence of histopathological alterations in the body wall and gastrointestinal tract, were also determined at 0, 14 and 56 days of exposure. The hematoxylineosin method was used to prepare slides for optic microscopy. Results of the study: Results have shown the bioaccumulation of metals and radionuclides, as well as, growth reduction in earthworms exposed to contaminated soil. Also after 14 days, the exposure to the contaminated soil, caused alterations such as disorganization and deformation of the tissues analyzed (except for the intestinal epithelium). More severe effects, like atrophy, degradation and necrosis were observed after 56 days of exposure, in all the tissues analyzed. Here we demonstrate that histological changes in the body wall and gastrointestinal tract are important endpoints to be used in the earthworm reproduction assay, as part of the risk assessment of contaminated areas. doi:10.1016/j.toxlet.2011.05.468

P1233 Are persistent organic pollutants associated with obesity, the metabolic syndrome or both? L. Lind 1, , D. Lee 2 , D.R. Jacobs 3 , S. Salihovic 4 , B. vanBavel 4 , P.M. Lind 5
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Acute and Internal Medicine, Department of Medicine, Uppsala University, Uppsala, Sweden, 2 Department of Preventative Medicine, School of Medicine, Kyungpook National University, Daegu, Republic of Korea, 3 Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, USA, 4 Mtm Research Center, School of Science and Technology, rebro University, rebro, Sweden, 5 Occupational and Environmental Medicine, Uppsala University, Uppsala, Sweden Purpose: It is well known that some obese persons do not have metabolic syndrome (MetS). Those subjects have been denoted Metabolically healthy obese. Since POP have been suggested to be linked both to obesity and the MetS, we investigated circulating POP levels regarding different combinations of BMI and the

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