Proligo Reagents

®

Nucleic acid solutions for genomic development
Phosphoramidites / Modifiers / Supports / Liquid Reagents

Welcome to the future of genomics

www.safcsupplysolutions.com

Strength In Our History
Our beginnings are aligned with the infancy of nucleic acid synthesis technologies. In 1981, Professor Hubert Köster founded Biosyntech GmbH, incorporating proprietary nucleic acid synthesis technologies in Hamburg, Germany. Since that time, the entire industry has undergone numerous transformations, and our nucleic acid chemistry expertise and manufacturing capabilities have remained at its forefront. Today, Proligo® Reagents from SAFC Supply Solutions® are recognized the world over for their quality. Our history of providing DNA and RNA synthesis reagents and supporting services is one customers have depended upon in their oglionucleotide programs for over 27 years.

1981

Biosyntech GmbH proprietary nucleic acid synthesis technologies founded by Professor Hubert Köster Biosyntech acquired by Milligen, a division of Millipore®, Inc. Transfer of Biosearch chemical operations from California to Hamburg, Germany First ISO 9001 biotechnology company certified in Germany Integration into PerSeptive Biosytems®, Inc. Acquisition of Controlled Pore Glass (CPG®) technology Manufacturing facilities improved to state-of-the-art PerSeptive Biosystems®, Inc. merges with Perkin-Elmer® Corporation Proligo® Reagents founded by SKW and NeXstar Proligo® Reagents multi-ton manufacturing capabilities introduced Proligo® Reagents purchased by Sigma-Aldrich, Inc. for its SAFC® custom manufacturing group

1986

1991

1993

1994

To place an order or inquire about custom manufacturing contact your local SAFC representative or visit www.safcsupplysolutions.com

1995

1996

1998

1998

2002

2005

Table of Contents
SAFC Supply Solutions® Proligo® Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Manufacturing, Purification & Filling ..................................2

Custom Manufacturing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Quality Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Quality Assurance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Packaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Pharmadite® for Pharmaceutical Applications . . . . . . . . . . . . . . . . . . . . . . . . . 8 DNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Fast Deprotection Chemistries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 RNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 2'O-Methyl RNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 DMT-2'Fluoro Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 Labels and Modifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Non-Standard Nucleosides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Phosphate-ON Phosphoramidite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 NPPOC Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Solid Supports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Controlled Pore Glass and Columns — CPG Free Flow . . . . . . . . . . . . . . . . 42 Columns for Synthesizers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 Amino-ON CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 Universal CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Liquid Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 Activator 42® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 Product List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

1

SAFC Supply Solutions® Proligo® Reagents

The Industry Leader in Manufacturing Amidites and Pharmidites®
A world-class provider of high-purity, high-yield nucleic acid solutions, SAFC Supply Solutions’ Proligo® Reagents brings revolutionary innovation and the reliability of outstanding regulatory compliance to diagnostics and pharmaceutical customers worldwide involved in genetic development programs. Our dedicated experts have extensive experience manufacturing key high performance raw materials for DNA and RNA oglionucleotide synthesis for diagnostics and pharmaceutical applications. As the largest manufacturer of amidites and pharmidites in the industry, Proligo Reagents’ extensive inventory of standard building blocks and custom manufacturing capabilities can meet the most demanding requirements. Our product range begins with highquality starting materials and includes: • HPLC-purified DNA & RNA phosphoramidite monomers to ensure consistent quality • Solvents (manufactured in the U.S. and in Hamburg, Germany) that increase yield through extremely low controlled water content and unique formulations • Complete scale-up, process development and analytical method development services • Complete control and documentation processes • A highly-qualified scientific staff with extensive backgrounds in nucleic acid products and specialty monomers • An ISO certified manufacturing facility built for amidites production backed by a solid audit track record • Phosphoramidite manufacturing capacity of over 5 tons per year • The dependability that your intellectual property will be treated with respect

Manufacturing, Purification & Filling
Manufacturing
Phosphoramidites and controlled pore glass (CPG) solid supports are produced in our state-of-the-art Hamburg, Germany manufacturing facilities. To serve the global market, standard formulation and custom requests for liquid formulations, solvents and reagents are produced in explosion-proof environments at the Hamburg site and at our Sheboygan, Wisconsin manufacturing facility in the U.S. Because we are a leading manufacturer, SAFC Supply Solutions has the flexibility to supply a wide variety of high-volume standard products as well as manufacture small-scale custom specialties. Dedicated labs and extensive process development and scale-up expertise compliments our multiple production suites while our large capacity ensures quick response time to satisfy ever-changing market demands. Flexible production comes from numerous midsize reactor trains (20-200L), glass-lined reactors to 1,000L, temperature ranges from -30ºC to 160ºC, and an integrated solvent delivery system.

HPLC Purification
DNA and RNA phosphoramidites are our specialty. We purify by preparative column chromatography on silica gel with medium to high press chromatography equipment. All materials undergo complete analytical support prior to their release with purification supported by: • Annual amidite purification capacity of over 5,000 kg • Large automated preparative HPLC with scales to 500 mm column diameter • DCM handling capability • Automated batch processing • State-of-the-art process visualization • Built-in Clean In Place (CIP) procedures

Filling
Customers appreciate our flexible packaging options and we can meet specific requirements for commercial synthesizers. In liquid fill, our completely segregated operations support glass bottle and drum containers. Automated powder fill lines include synthesis column assembly and custom packaging — from milligrams to tons. Our comprehensive services include global warehousing and storage for our customer’s manufactured products.

2

Custom Manufacturing
SAFC Supply Solutions’ Proligo Reagents is a skilled custom synthesis manufacturer for nucleic acids products. Our strong history in the genomics industry and solid manufacturing foundation combine with SAFC Supply Solutions’ industry-leading regulatory knowledge and allows us to manufacture materials to the highest quality levels demanded by the pharmaceutical and diagnostics industries. If you are looking for a unique modifier, you can remain confident our staff has the expertise to support your program development, scale-up, analytical, quality assurance, packaging and distribution, and will never compromise your intellectual property. We understand the efficiencies gained by proper project management. We realize the value working with a knowledgeable partner brings to every step of process development and we know the importance of quality assurance. We understand the science and have the capabilities to support your cGMP synthesis operations. SAFC Supply Solutions Proligo Reagents is the industry’s leading specialist in manufacturing:

• Nucleoside phosphoramidites and other activated monomers, nucleobase modifications, non-natural nucleosides, alternative protective groups, backbone modifications • Solid phase synthesis supports loaded with unusual base; alternative linkage • Modifiers and specialties • Solutions and solvents for DNA/RNA synthesizers

SAFC handles these critical materials for large-scale production
• Phosphorous trichloride and organic phosphorous (III) chlorides • Phosphorous oxychloride • Carbonic acid chlorides • Trimethylchlorosilane • Trimethylsilyl triflate • Dimethylamine

SAFC Capabilities
Process/Technology
Phosphoramidation

Features
Introduction of the diisopropyl-β-cyanoethyl phosphoramidite group

Reactions/Examples

N R O H R O P O C N

Tritylation (Dimethoxytritylation)

Introduction of triphenylmethyl protective groups
R O H R O C O C H 3

O C H 3

Silylation/Desilylation

Introduction or removal of silyl protective groups
HO O

Base
S i O

O O

Base

S i O H O O H
R

O H

N- and O-Acylation

Introduction of nucleobase and ribose protective groups
N

N H 2

C N H

O

N O N O R ib o s e R ib o s e N

Nucleobase Conversion

Transformation of easily assessable nucleosides to nucleosides with other nucleobases
O H N

O C H 3 N

N H 2 C H 3

N

O

N

S u g ar

S u ga r

Preparative Chromatography

High-pressure, fully automated separation on normal phase silica gel including freely programmable gradient elution

>99% purity

Surface Chemistry

Loading and capping of functionalized glass and organic polymer surfaces
O N H

R

O O

Base

O 2 N H O

3

SAFC Supply Solutions® Proligo® Reagents

Compliance
Strong ties in supplying materials to the pharmaceutical industry have helped our customers around the world to understand compliance requirements of their new materials. Our strict quality control procedures include full traceability, complete documentation and change control notification. All our manufacturing processes are monitored and reviewed, with controls in place at each critical parameter to ensure compliance.

Quality Control
SAFC has rigorous QC protocol for every product we produce to identify purity levels against set specifications. We can provide custom QC testing upon request. We routinely use these state-of-theart analytical methods: • RP & SAX HPLC • LC-MS , • NMR (31P 1H,
13

C,

19

F)

• Nucleic acid synthesis test • FT-IR • Titration & “Wet Chemistry” • UV/VIS • Karl Fischer water analysis • Mercury porosimetry

Quality Assurance
As a trusted partner to the oglionucloetide market, SAFC assists its customers in new product development. We know that developing materials includes having insight to their quality requirements. As a result, our company operates in a culture that fosters continuous process improvement and has quality systems in place to provide complete traceability on all our raw materials, processes and procedures. We serve customers requesting audits with complete documentation and files. Our quality assurance department routinely accepts requests for documentation, including Certificates of Origin. Our Hamburg facility was one of the first in the industry to be ISO 9001 certified (1993), and we have synthesized amidites under ISO9000:2000 since that time.

4

Packaging
Flexible packaging options are available to meet virtually any synthesizer requirement, including , ABI® 394, ABI® 3900, Expedite™ AKTA and Mermade. Inquire for custom packaging requirements. To complete the single-source supplier advantage, from product order to delivery, SAFC has liquid filling and blending capabilities at its Hamburg and Sheboygan, Wisconsin facilities with capabilities from bottles to 1,400 L returnable cylinders. We have distribution offices around to globe to offer our customers the convenience of a worldwide distribution network with the advantages of dedicated airfreight space, chemical transport regulations and local delivery knowledge.

Standard Bottles and Characteristics
Description
15 ml septum 60 ml septum 100 ml septum 1 oz 20/400 (30 ml) 2 oz 20/400 (60 ml) 8 oz 24/400 (240 ml) 8 oz 28/400 (240 ml) 16 oz 28/400 (480 ml) 1 g CPG bottle (10 ml) 10 g CPG bottle (50 ml) V-Vial 20/400 2.5 L GL45 4 L 38/430

Height (cm)
4.5 10.0 9.5 7.8 9.4 13.7 13.7 17.0 5.8 9.5 6.0 29.6 35.3

Diameter (cm)
2.9 3.3 5.1 3.1 3.8 6.0 6.0 7.2 2.5 4.0 2.0 13.5 16.1

Quantity (powder)
0.25 g, 0.5 g, 1 g 1 g, 2 g, 4 g 5 g, 10 g 0.25 g, 0.5 g, 1 g 1 g, 2 g 10 g 5 g, 10 g 10 g, 20 g 1g 10 g 0.1 g, 0.25 g

Quantity (liquid reagents)

100 ml

180 ml, 200 ml 200 ml 450 ml

2500 ml 4000 ml

15 ml septum

60 ml septum

100 ml septum

1 oz 20/400

2 oz 20/400

8 oz 24/400

8 oz 28/400

16 oz 28/400

1 g CPG

10 g CPG

V-Vial 20/400

2.5 L GL45

4 L 38/430

Bulk Packaging

5

SAFC Supply Solutions® Proligo® Reagents

Worldwide Packaging Options
Standard drums and characteristics
Description
1400 L Returnable Container 200 L Returnable Drum, Standard 200 L Returnable Drum, Level Sensor 200 L Drum, Disposal 50 L Returnable Drum 45 L Returnable Drum 30 L Returnable Drum 30 L Drum, Disposal 18 L Returnable Drum

Capacity
1400 L 200 L 200 L 180 L 45 L 45 L 25 L 25 L 18 L

Total Capacity
1500 L 225 L 225 L 200 L 50 L 50 L 30 L 30 L 20 L

Weight
247 kg 43 kg 43 kg 21 kg 12 kg 11 kg 10 kg 3.4 kg 6.6 kg

Height
1960 mm 970 mm 970 mm 884 mm 600 mm 600 mm 400 mm 560 mm 442 mm

Diameter
1200 mm 600 mm 600 mm 585 mm 363 mm 363 mm 380 mm 280 mm 278 mm

Wall thickness
N/A 2 mm 2 mm 1 mm 1.5 mm 1.5 mm 1.5 mm 0.5 mm 1.5 mm

Some packaging options may not be available in your region. Please contact your sales representative to inquire.

Additional Packaging Options (U.S. Only)
Standard drums and characteristics
Description
20 L Returnable Drum 50 L Returnable Drum 200 L Returnable Drum 200 L Returnable Drum, Level Sensor

Capacity
20 L 50 L 200 L 200 L

Total Capacity
22 L 55 L 220 L 220 L

Weight
8 kg 15 kg 47 kg 47 kg

Height
20 inches 23 inches 38 inches 38 inches

Diameter
11 inches 16 inches 24 inches 24 inches

Wall thickness
1.5 mm 1.5 mm 2 mm 2 mm

Other containers available upon custom packaging request, please inquire your sales representative.

6

Products
Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Pharmadite® for Pharmaceutical Applications . . . . . . . . . . . . . . . . . . . . . . . . . 8 DNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Fast Deprotection Chemistries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 RNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 2'O-Methyl RNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 DMT-2'Fluoro Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 Labels and Modifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Non-Standard Nucleosides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Phosphate-ON Phosphoramidite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 NPPOC Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Solid Supports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Controlled Pore Glass and Columns — CPG Free Flow . . . . . . . . . . . . . . . . 42 Columns for Synthesizers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 Amino-ON CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 Universal CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Liquid Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 Activator 42® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 Product List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

7

Products

Pharmadite® for Pharmaceutical Applications

Pharmadite for Pharmaceutical Applications
The Pharmadite product line represents a new class of standard protected DNA and RNA phosphoramidites designed with highly controlled impurity profiles and exceptional overall purity. Pharmadite products fulfill all the requirements of starting materials for the manufacture of active pharmaceutical ingredients as defined in the EMEA “Note for guidance on chemistry of the new active substance,”* making them suitable building blocks for oligonucleotide drugs. Pharmadite amidites are manufactured in scales of up to multi-hundred kilos, under certified ISO 9001 quality systems at SAFC Supply Solutions manufacturing facility in Hamburg, Germany. We start with traceable, non-animal and very pure raw materials, use highly controlled synthesis and purification processes, validated analytical methods and cleaning processes, all governed with strict change control and documentation to yield unprecedented high quality products with purity ratings at 99.5% or higher for DNA and 99.0% or higher for RNA. All remaining impurities, if present, are identified and characterized at a level of 0.1%. The high level of control and documentation imposed at every step of the production process support regulatory requirements to make Pharmadite amidites ideal for pharmaceutical applications.

Features
• Consistent quality

Benefits
• Reproducible oligonucleotide synthesis • Accordance with regulatory guidelines

• Suitability as starting material for API manufacture - Raw material control and traceability - Process control - Validated analytical methods - Tight specifications - Known impurity profiles • Very high purity • Non-animal origin of starting materials • Available in large scale • Supply through our worldwide supply chain

• High production efficiency • TSE safety • Secure supply • Long-term reliability

Manufacturing
Pharmadite amidites are prepared from DMTprotected nucleosides and the phosphitylating agent “bis-amidite.” The crude phosphoramidites are then purified by preparative HPLC, dried and packaged. To ensure the highest quality, our production process begins with starting materials that possess stringent specifications and defined impurity profiles. Our HPLC production line is cleaned using validated cleaning methods and cleaning verification is performed prior to each batch. Reactions are conducted at a minimum synthesis scale of 50 kg per batch. Each processing step is monitored using analytical in-process controls, including HPLC.

QC Starting Matl. Specifications

In-Process Controls

In-Process Controls

QC Bulk Specifications

QC Packaged Matl Specifications

DMTdNucleoside

Crude Phosphoramidite

Purified Phosphoramidite

Bulk Phosphoramidite

Packaged Phosphoramidite

Synthesis

HPLC Purification

Drying

Packaging

*EMEA: European Medicines Agency, http://www.emea.eu.int

8

Specifications
Pharmadite amidites are characterized by their exceptional purity and well-defined impurity profile. In particular, they are essentially free from contaminants which interfere in coupling reactions, such as other nucleosidic or non-nucleosidic phosphoramidites (P(III)-contaminants).

DNA Pharmadites Characteristic
Appearance

Acceptance Limit
white to off-white powder or granules

RNA Pharmadites Characteristic
Appearance

Acceptance Limit
white to off-white powder or granules

Appearance of Solution Solubility Identification HPLC Purity Specified impurities Single Unspecified Impurity
31

≤10 Hazen (c = 0.2 M in ACN) clear solution (c = 0.2 M in ACN) conforms ≥99.5% area ≤0.3% area, see impurities table ≤0.1% area ≥99.5% area ≤0.1% area

Appearance of Solution Solubility HPLC Identification HPLC Purity Specified Impurities Single Unspecified Impurity
31

≤10 Hazen (c = 0.2 M in ACN) clear solution (c = 0.2 M in ACN) conforms ≥99.0% area See impurities table ≤0.1% area ≥99.0% area ≤0.3% area

P NMR Purity P NMR P(III) Impurities

P NMR Purity P NMR P(III) Impurities

31

31

(@ 100 to 169 ppm)
31

(@ 100 to 169 ppm) ≤0.5% area
31

P NMR P(V) Impurities

P NMR P(V) Impurities

≤1.0% area

(@ -25 to 99 ppm)
31

(@ -25 to 99 ppm) not detected: S/N < 2.5
31

P NMR ≥170 ppm Impurities

P NMR ≥170 ppm Impurities

not detected

(@ 170 to 225 ppm) Residual Solvent Content Water Content Origin of Nucleoside ≤3.0% wt ≤0.40% wt non-animal origin

(@ 170 to 225 ppm) Residual Solvent Content Water Content Origin of Nucleoside ≤3.0% (w/w) ≤0.40% (w/w) non-animal origin

All remaining impurities in Pharmadite amidites, if present, are identified, characterized and quantified. The impact of any such defined impurities on the synthesis of oligonucleotides is well understood and has been shown to be insignificant.

Specified Impurities of DNA Pharmadites
DMT-dNucleoside-cyanoethyl-H-phosphonate DMT-dNucleoside phosphoramidate DMT-dNucleoside-diisopropylamino-H-phosphonate 03'-Benzoyl-O5'-DMT-dC(bz)

A
≤0.3% ≤0.3% not specified not applicable

C
≤0.3% ≤0.3% not specified ≤0.3%

G
≤0.3% ≤0.3% ≤0.3% not applicable

T
≤0.3% ≤0.3% not specified not applicable

Specified Impurities of RNA Pharmadites
2'O-Amidite-3'O-TBDMS-5'O-DMT-rNucleoside DMT-rNucleoside TBDMS DMT-rNucleoside TBDMS-cyanoethyl-H-phosphonate DMT-rNucleoside TBDMS-phosphoramidate

A
≤0.3% area ≤1.0% area ≤1.0% area ≤1.0% area

C
≤0.3% area ≤1.0% area ≤1.0% area ≤1.0% area

G
≤0.3% area ≤1.0% area ≤1.0% area ≤1.0% area

U
≤0.3% area ≤1.0% area ≤1.0% area ≤1.0% area

9

Products

Pharmadite® for Pharmaceutical Applications

Quality system and production process designed to meet pharmaceutical standards.
Control of starting materials and production process is a key factor in the synthesis procedure of RNA Pharmadite. The phosphitylating reagent is controlled at a level of 0.1% P(III)- impurities. RNA Pharmadite amidites are purified by preparative HPLC in a highly automated purification plant. ISO 9001 certified since 1993, we guarantee reproducible quality with predictable and controlled production: • Robust process parameters • In-process controls with specifications • Batch integrity • Change control • Batch record review • Process database and trend analysis • Validated cleaning procedures

DNA Pharmadites
Catalog No.
A111P00-C C111P00-C G111P00-C T111P00-C

Description
DMT-dA(bz) Pharmadite DMT-dC(bz) Pharmadite DMT-dG(ib) Pharmadite DMT-dT Pharmadite

RNA Pharmadites
A211P00-C C213P00-C G211P00-C U211P00-C DMT-2'O-TBDMS-rA(bz) Pharmadite DMT-2'O-TBDMS-rC(ac) Pharmadite DMT-2'O-TBDMS-rG(ib) Pharmadite DMT-2'O-TBDMS-rU Pharmadite

dA(bz) Phosphoramidite

dG(ib) Phosphoramidite

dC(bz) Phosphoramidite

dT Phosphoramidite

10

DNA Phosphoramidites

DNA Phosphoramidites
SAFC Supply Solutions’ world-class expertise in nucleic acid synthesis began in 1983 when Dr. Hubert Köster first commercialized the synthesis of ß-cyanoethylphosphoramidites. Since then,

Standard DNA Phosphoramidites (cont.)
Compatible with MerMade Instruments (8oz 28/400 bottle)
A111085-06 C111085-06 G111085-06 T111085-06 DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite 6x5g 6x5g 6x5g 6x5g 6 x 10 g 6 x 10 g 6 x 10 g 6 x 10 g

the company has refined and perfected the
A111028-06

production of DNA and RNA phosphoramidites.
C111028-06

Today, SAFC Supply Solutions offers the highest
G111028-06

quality phosphoramidites in the industry.

T111028-06

Compatible with Äkta and Oligo Pilot Instruments (100 ml septum bottle)

DNA Phosphoramidites
Key Features of DNA Phosphoramidites • Exocyclic amine functions are protected by a benzoyl group (dA(bz) and dC(bz)) or isobutyryl group (dG(ib)) • Recommended cleavage and deprotection conditions are 8 hours at 55°C or 24 hours at room temperature using concentrated ammonia solution, for standard base-protected oligonucleotides • The high coupling efficiency of Proligo Reagents’ DNA phosphoramidites leads to high-yield, high-quality oligonucleotides

A111005-01 C111005-01 G111005-01 T111005-01 A111005-06 C111005-06 G111005-06 T111005-06 A111010-01 C111010-01 G111010-01 T111010-01

DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite

1x5g 1x5g 1x5g 1x5g 6x5g 6x5g 6x5g 6x5g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g

Bulk Quantities (16 oz 28/400 bottle)
A111021-06 C111021-06 DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite 6 x 10 g 6 x 10 g 6 x 10 g 6 x 10 g 6 x 20 g 6 x 20 g 6 x 20 g 6 x 20 g

Standard DNA Phosphoramidites
Compatible with Expedite and Polygen Instruments Catalog No.
A111081-12 C111081-12 G111081-12 T111081-12 A111082-12 C111082-12 G111082-12 T111082-12

G111021-06 T111021-06

Description
DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite

Unit
12 x 1 g 12 x 1 g 12 x 1 g 12 x 1 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 2 g

A111020-06 C111020-06 G111020-06 T111020-06

Customized packaging
Bulk packaging up to multiple kg per container and customized packaging with alternative quantities of amidites are available upon request.

Compatible with ABI Instruments
A111031-12 C111031-12 G111031-12 T111031-12 A111032-12 C111032-12 G111032-12 T111032-12 A111064-12 C111064-12 G111064-12 T111064-12 DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite 12 x 1 g 12 x 1 g 12 x 1 g 12 x 1 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 4 g 12 x 4 g 12 x 4 g 12 x 4 g

11

Products

Fast Deprotection Chemistries

Fast Deprotection Chemistries
The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. We offer various fast deprotection chemistries for the rapid and high-yield synthesis of high-purity.

Overview
Fast deprotection methods
Substitution of dC(bz) with dC(ac) (Beckmann method)

Monomers

Cleavage and deprotection reagents
AMA reagent**

Time/ Temperature
10 min. at 65°C

dA(bz), dC(ac), dG(ib), dT

1. Substitution of dC(bz) with dC(ac) — Beckman licenced method
Proligo Reagents’ portfolio of oligonucleotide synthesis reagents with acetyl-protected phosphoramidtes and CPG has a license for Beckman Coulters’s fast deprotection chemistry.
®

TAC chemistry

dA(tac), dC(tac), dG(tac), dT

Concentrated ammonia*

15 min. at 55°C or 2 hrs. at room temp. 5 min. at 65°C or 30 min. at room temp. 2 hrs at 55°C or 1 hr. at 65°C

AMA reagent**

dG(dmf) method

dA(bz), dC(bz), dG(dmf), dT

Concentrated ammonia*

Key Features of Acetyl-protected phosphoramidtes
• Ultrafast deprotection (10 minutes at 65°C) • Key component in oligonucleotide synthesis • Industry standard for fast deprotection chemistry • Operating for AMA methods without changes • Consistent lot-to-lot high purity and performance • Manufactured under a certified ISO 9001 quality system

Substitution of dC(bz) with dC(tac)

dA(bz), dC(tac), dG(ib), dT

AMA reagent**

10 min. at 65°C

* ≥25% ammonia in water ** Mixture of ≥25% ammonia in water with 40% aqueous methylamine I/I, v/v

2. TAC Chemistry
Substitution of standard protecting groups with the labile TAC (tert.butylphenoxyacetyl) protecting group results in ultra-fast and easy deprotection under mild conditions, suitable for oligonucleotides with base-labile monomers and reporters as well as in-situ synthesis schemes on glass surfaces.

O OMe HN N MeO O O O O P O N CN N

dC(ac) Phosphoramidite

dC(tac) Phosphoramidite

dG(dmf) Phosphoramidite

12

Key features of TAC Chemistry
• Deprotection of the TAC group is ultra-fast: complete deprotection in concentrated ammonia occurs within 15 minutes at 55°C or two hours at room temperature • Compatible with the AMA deprotection reagent (a mixture of ≥25% ammonia in water with 40% aqueous methylamine I/I, v/v) • Highly soluble in acetonitrile. No need to add co-solvents such as dimethylformamide or methylene chloride • Suitable for the synthesis of oligomers with base-labile units e.g., dyes and modifiers, because of less exposure to ammonia and the possibility of room temperature deprotection • No change is required in the reagents commonly used for DNA synthesis, except that Proligo Reagents’ Fast Deprotection Cap A solution is used instead of Cap A solution • The application of dA(tac) minimizes depurination and improves the quality of oligonucleotides

4. Substituting the dC Protecting Group
Changing the dC protecting group to the TAC protecting group leads to rapid synthesis of highpurity and high-yield oligonucleotides. Substituting the commonly employed dC(bz) monomer by the dC(tac) monomer enables the application of ultrafast deprotection with the AMA reagent and provides a high-throughput method of oligonucleotide synthesis.

Key Features of Substituting the dC Protecting Group
• The deprotection of oligonucleotide synthesis products with the AMA reagent is ultra-fast: complete deprotection requires 10 minutes at 65°C • Side reactions at C-monomers through transamination are eliminated • Not compatible with some base-labile modified nucleosides • dC(tac)-amidite can directly substitute for dC(bz)-amidite • No change is required in the reagents commonly used for DNA synthesis: acetonitrile is used to dissolve the amidite. The standard acetic anhydride capping reagent can be employed

3. dG(dmf) Method
Changing the dG protecting group to the dimethylformamidine (dmf) base-protecting group enables rapid synthesis of high-purity, high-yield oligonucleotide, thus increasing the efficiency of high-throughput production.

Key Features of dG(dmf)
• dG(dmf) is deprotected faster than the conventional dG(ib): the deprotection time in concentrated ammonia is reduced to 2 hours at 55°C or 1 hour at 65°C • The dG(dmf)-monomer is especially suitable for G-rich sequences: incomplete deprotection is greatly reduced in comparison with the conventional dG(ib)-monomer • dG(dmf)-amidite is as stable in solution as the standard dA(bz)-, dC(bz)- and dT-amidites • dG(dmf)-amidite can directly substitute for dG(ib)-amidite • No change is required in the reagents commonly used for DNA synthesis (except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should be employed)

13

Products

Fast Deprotection Chemistries

Fast Deprotection Phosphoramidites
Compatible with Expedite and Polygen Instruments Catalog No.
C113081-12 C113082-12 A112081-12 C112081-12 G112081-12 A112082-12 C112082-12 G112082-12 G115081-12 G115082-12

Description
DMT-dC(ac) Amidite DMT-dC(ac) Amidite DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dG(dmf) Amidite DMT-dG(dmf) Amidite

Unit
12 x 1 g 12 x 2 g 12 x 1 g 12 x 1 g 12 x 1 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 1 g 12 x 2 g

Compatible with ABI Instruments
A112031-12 C113031-12 C113032-12 C112031-12 G112031-12 A112032-12 C112032-12 G112032-12 A112064-12 C112064-12 G115031-12 G115032-12 G115064-12 DMT-dA(tac) Amidite DMT-dC(ac) Amidite DMT-dC(ac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(dmf) Amidite DMT-dG(dmf) Amidite DMT-dG(dmf) Amidite 12 x 4 g 12 x 1 g 12 x 2 g 12 x 1 g 12 x 1 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 4 g 12 x 4 g 12 x 1 g 12 x 2 g 12 x 4 g

Compatible with MerMade Instruments (8oz 28/400 bottle)
C113085-06 C112028-01 C112085-06 G115028-06 G115085-06 DMT-dC(ac) Amidite DMT-dC(tac) Amidite DMT-dC(tac) Amidite DMT-dG(dmf) Amidite DMT-dG(dmf) Amidite 6x5g 1 x 10 g 6x5g 6 x 10 g 6x5g

Compatible with Äkta and Oligo Pilot Instruments (100 ml septum bottle)
A112010-01 C112010-01 G112010-01 G115005-01 DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dG(dmf) Amidite 1 x 10 g 1 x 10 g 1 x 10 g 1x5g

Bulk Quantities (16 oz 28/400 bottle)
C113020-06 C112021-06 C112020-06 G115021-06 DMT-dC(ac) Amidite DMT-dC(tac) Amidite DMT-dC(tac) Amidite DMT-dG(dmf) Amidite 6 x 20 g 6 x 10 g 6 x 20 g 6 x 10 g

14

RNA Phosphoramidites, 2'O-Methyl RNA Phosphoramidites

RNA Phosphoramidites, 2'O-Methyl RNA Phosphoramidites
Modern phosphoramidite mediated synthesis has enabled routine high yielding preparations of RNA- and 2'O-Methyl RNA oligonucleotides. High quality RNA amidites are key to low failure rates, high biological activity of synthesis products and cost effectiveness. SAFC Supply Solutions provides high purity RNA amidites and 2'OMethyl RNA amidites. RNA monomers carry the industry standard 2'-TBDMS protective group.

RNA Phosphoramidites
RNA plays a pivotal role in biological systems due to its numerous functions in the transfer and processing of genetic information. The unique properties of RNA have stimulated the development of a variety of applications in diagnostics and therapeutics, as well as in basic molecular biology research where RNA can be used as: • Catalytic agents (ribozymes) • Affinity ligands (aptamers) • Agents to induce gene silencing (RNA interference) RNA interference (RNAi) has become a popular tool for the sequence-specific inhibition of gene expression and can be used in target validation and other drug development techniques. The most convenient method to provide sequence-specific RNA oligonucleotides is chemical synthesis on a solid support with RNA phosphoramidites and RNA CPG, analogous to DNA synthesis.

Standard RNA Amidites
• Industry standard 2'-TBDMS protective group • Consistent lot-to-lot purity and performance • Compatible with deprotection methods based on methylamine or AMA • Standard RNA amidites provide excellent coupling results when used with ETT or BTT as activator; best results are obtained with Activator 42 • Capping with standard acetic anhydride capping reagent rather than with Fast Deprotection Cap A • Manufactured under a certified ISO 9001 quality system

RNA Phosphoramidites with Fast Deprotection Chemistry
Advantages of synthesis with TAC-protected RNA phosphoramidites
• Increased synthesis yield and reduced purification efforts • Dramatically reduced deprotection times • Minimized exposure to alkaline deprotection medium • Minimized chain degradation during deprotection

15

Products

RNA Phosphoramidites, 2'O-Methyl RNA Phosphoramidites

RNA Synthesis
The synthesis cycle for RNA oligonucleotides consists of the same series of reactions as the cycle that is employed for DNA monomers. However, the rate of coupling for RNA monomers is slower, compared to that of DNA monomers (for RNA monomers a coupling time of 10 minutes or 6 minutes using Activator 42 is recommended compared to 90 seconds for DNA monomers). With the exception of the monomers and supports, RNA synthesis is accomplished with the same reagents as DNA synthesis. All RNA phosphoramidites are diluted with dry acetonitrile. deprotection of the RNA oligomer, e.g., with a solution of tetrabutylammonium fluoride (TBAF) in tetrahydrofane (THF) or with triethylamine hydrofluoride.

RNA Phosphoramidites
Compatible with Expedite and Polygen Instruments

Catalog No.
A211081-01 C213081-01 G211081-01 U211081-01

Description
DMT-2'O-TBDMS-rA(bz) Amidite DMT-2'O-TBDMS-rC(ac) Amidite DMT-2'O-TBDMS-rG(ib) Amidite DMT-2'O-TBDMS-rU Amidite

Unit
1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g

Compatible with ABI Instruments
A211031-01 DMT-2'O-TBDMS-rA(bz) Amidite DMT-2'O-TBDMS-rC(ac) Amidite DMT-2'O-TBDMS-rG(ib) Amidite DMT-2'O-TBDMS-rU Amidite DMT-2'O-TBDMS-rA(bz) Amidite DMT-2'O-TBDMS-rC(ac) Amidite DMT-2'O-TBDMS-rG(ib) Amidite DMT-2'O-TBDMS-rU Amidite 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1x1g 1x1g 1x1g 1x1g

User Instructions
RNA monomers feature hydroxyl groups at the 2'position. In order to prevent the formation of unnatural 2'-5' phosphodiester bonds during chain elongation, the 2'OH group is protected with a trialkyl-silyl group, tert-butyldimethylsilyl (TBDMS). The TBDMS group is stable under the acidic conditions used to remove the DMT group during the synthesis cycle, but can be removed by a variety of methods after cleavage and

C213031-01 G211031-01 U211031-01 A211061-01 C213061-01 G211061-01 U211061-01

Other quantities and packaging are available upon request.

OMe N MeO O N O

O HN N N

O OMe HN N MeO O O O OTBDMS O P O N CN N

OTBDMS O P O N CN

DMT -rA(bz) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C53H66N7O8PSi 988.2 ≤-10°C

DMT -rC(ac) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C47H64N5O9PSi 902.1 ≤-10°C

DMT-rAdenosine(N6-bz)(2'O-TBDMS)-ß-Cyanoethylphosphoramidite

DMT-rCytidine(N4-ac)(2'OTBDMS)-ß-Cyanoethylphosphoramidite

OMe N MeO O N O

O NH N N H O MeO

OMe HN O O O

O

N

OTBDMS O P O N CN

OTBDMS O P O N CN

DMT -rG(ib) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C50H68N7O9PSi 970.2 ≤-10°C

DMT -rU Phosphoramidite
Chemical Formula: Formula Weight: Storage: C45H61N4O9PSi 861.1 ≤-10°C

DMT-rGuanosine(N2-ib)(2'O-TBDMS)-ß-Cyanoethylphosphoramidite

DMT-rUridine(2'O-TBDMS)-ß-Cyanoethylphosphoramidite

16

User Instructions (continued)
1. Use anhydrous acetonitrile (water content ≤30 ppm) as diluent. It is important to maintain anhydrous conditions while dissolving RNA amidites in acetonitrile. For use on PE 8900 instruments, add 10 ml of acetonitrile to 0.5 g RNA monomer, to obtain a concentration of 50 mg/ml. For use on PE 390 series instruments, add 5 ml acetonitrile to 0.5 g RNA monomer to obtain a concentration of 100 mg/ml. Gently swirl the vial until the powder is completely dissolved. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. Enter the sequence of the RNA oligonucleotide you wish to synthesize. A minimum coupling time of 10 minutes or 6 minutes using Activator 42 is recommended for 2'-TBDMS protected RNA amidites. Proceed as you would with a standard DNA oligonucleotide synthesis. Depending on your intended further use of the oligomer, you can choose either DMT-On or DMT-Off procedures. The coupling efficiency of RNA monomers may be determined by standard dimethoxytrityl cation assays. Cleave from the support and deprotect the RNA oligonucleotide with a mixture of concentrated ammonia and ethanol 3/1, v/v, at 55°C for 8 hours, or at room temperature for 24 hours. Alternatively, AMA reagent (concentrated ammonia/40% aqueous methylamine 1/1, v/v) can be employed for 10 minutes at 65°C. It is essential to employ sterile conditions from this step forward. Always use sterilized water: preferably water recently treated with 9. DEPC (diethyl pyrocarbonate, stir 1L of HPLC grade water with 100 l DEPC overnight and autoclave twice) and use baked glassware (250°C+ for more than 4 hours). Transfer the supernatant solution of the RNA oligonucleotide into a separate vial. The yield of the RNA oligonucleotide can be improved by rinsing the support with ethanol/acetonitrile/ water 3/1/1, v/v, and combining the oligonucleotide solution with the washing solution. Evaporate to dryness.

2.

3.

4.

5.

6.

10. Add a 1 M solution of tetrabutylammonium fluoride (TBAF) in THF and incubate for 24 hours at room temperature. The deprotection time can be shortened to 6 hours if a TBAFsolution, with water content less than 5%, w/w, is employed. Following deprotection, add an equal volume of 1 M TEAA buffer pH 7, followed by another volume of water. Alternatively, deprotection can be accomplished using a mixture of neat triethylamine trihydrofluoride, triethylamine and N-methylpyrrolidon, 4/3/6, v/v, which can be employed for 90 minutes at 65°C. The deprotection reaction is quenched by the addition of an equal volume of water in this case. Note that the application of triethylamine trihydrofluoride in the DMT-On mode will lead to detritylation, due to the acidity of the reagent. 11. Desalt the RNA oligonucleotide by using a desalting matrix such as Sephadex® G25, an ion exchange cartridge, or a reversed phase purification cartridge. Optimal conditions for desalting vary greatly with the employed matrix/product. Conditions recommended by the manufacturer should generally be applied. The lyophilized crude RNA oligonucleotide product can be purified by AX-HPLC or by preparative gel electrophoresis.

7.

8.

17

Products

RNA Phosphoramidites, 2'O-Methyl RNA Phosphoramidites

Key Features of TAC-Protected RNA Phosphoramidites
• Base protected by a tert-butylphenoxyacetyl (TAC) group, in the same manner as DNA phosphoramidites • Uses the same synthesizer protocols as recommended for bz/ib protected RNA monomers • Follows the established DNA synthesis cycle, but with prolonged coupling times due to steric hindrance • Same liquid reagents are used throughout the synthesis cycle as in DNA synthesis, except that Fast Deprotection Cap A reagent must be employed with Proligo Reagent’s TACprotected phosphoramidites • Fast Deprotection Cap A contains tertbutylphenoxyacetyl acetic anhydride (tac2O) in tetrahydrofuran, which ensures that the displacement of tert-butylphenoxyacetyl (TAC) on guanine bases does not occur • Cleavage and deprotection procedures are comparable with those of DNA synthesis, with an additional step to remove the 2'OH protecting group • 2'OH function is protected by a tertbutyldimethylsilyl (TBDMS) group to prevent derivatization and degradation during the synthesis cycle Although our TAC-protected RNA monomers display sufficient stability in solution over several days, we recommend to reconstitute fresh amidites after 6 days on the instrument to achieve optimal results.

O OCH3 N N O N HN N O

CH3O

O

O N C O P

OTBDMS N(iPr)2

rA(tac) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C58H76N7O9PSi 1074.4 ≤-10°C

DMT-rAdenosine (N6-tac)(O2'-TBDMS)-ß-Cyanoethylphosphoramidite
O OCH3 N CH3O O O O N HN O

O N C O P

OTBDMS N(iPr)2

rC(tac) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C57H76N5O10PSi 1050.3 ≤-10°C

DMT-rCytidine(N4-tac)(O2'-TBDMS)-ß-Cyanoethylphoshoramidite
OCH3 N N O N

O NH N H O O

CH3O

O

O N C O P

OTBDMS N(iPr)2

rG(tac) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C58H76N7O10PSi 1090.3 ≤-10°C

DMT-rGuanosine(N2-tac)(O2'-TBDMS)-ß-Cyanoethylphosphoramidite
OCH3 HN CH3O O O O N

O

O N C O P

OTBDMS N(iPr)2

rU Phosphoramidite
Chemical Formula: Formula Weight: Storage: C45H61N4O9PSi 861.1 ≤+10°C

DMT-rUridine(O2'-TBDMS)-ß-Cyanoethylphosphoraamidite

18

Fast Deprotection RNA Phosphoramidites
Compatible with Expedite and Polygen Instruments Catalog No.
A212081-01 C212081-01 G212081-01 U211081-01

Description
DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite

Unit
1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g

Compatible with ABI Instruments
A212031-01 C212031-01 G212031-01 U211031-01 A212061-01 C212061-01 G212061-01 U211061-01 DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1x1g 1x1g 1x1g 1x1g

Compatible with MerMade Instruments
A212028-06 C212028-06 G212028-06 U211028-06 DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite 6 x 10 g 6 x 10 g 6 x 10 g 6 x 10 g

Bulk Quantities
A212010-01 C212010-01 G212010-01 U211010-01 DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g

Customized packaging
Bulk packaging up to multiple kg per container and customized packaging with alternative quantities of amidites are available upon request.

19

Products

RNA Phosphoramidites, 2'O-Methyl RNA Phosphoramidites

RNA Supports
Final: Cleavage/Deprotection

1. Deblocking
D MT O O Bas e HO O Bas e

2. Activation and Coupling

D MT O OTBD MS O OTBD MS

O

O

Base

Solid Support

Solid Support
O OTBDMS N (iPr) 2 N C P

The supports for RNA synthesis consist of an RNA nucleoside covalently attached through either the 2'- or the 3'-position to controlled pore glass (CPG). The remaining free hydroxyl group is protected with a base-labile acyl group. The pore size of Proligo Reagents’ CPG for RNA synthesis is 500Å. Proligo Reagents offers ready-to-use synthesis columns for RNA synthesis at 1 mol scale.

D MT

O

O

Base

Start next cycle: 1. Deblocking

O

RNA Synthesis
D MT O O Bas e

O N C O P O O

OTBDMS O O Base N C O P O O Base OTBD MS

4. Oxidation
O OTBD MS O

Solid Support
O O O

OTBD MS

3. Capping

Solid Support

O

Base

The synthesis cycle for RNA oligonucleotides consists of the same series of reactions as the cycle that is employed for Fast Deprotection DNA monomers. However, the rate of coupling for RNA monomers is slower, compared to that of DNA monomers (a coupling time of 10 minutes for RNA monomers is recommended compared to 90 seconds for DNA monomers). With the exception of the monomers and supports, RNA synthesis is accomplished with the same reagents as DNA synthesis. All RNA phosphoramidites from Proligo Reagents are diluted with dry acetonitrile. Fast Deprotection Cap A is employed to prevent the transacylation of guanosine bases, similar to synthesis of tertbutylphenoxyacetyl (TAC) DNA monomers.

O

OTBD MS

Solid Support

Fast Deprotection RNA Synthesis Cycle

RNA Monomers TAC RNA Monomers
RNA oligonucleotides prepared from TAC protected RNA monomers can be base-deprotected under very mild conditions. Recommended cleavage and deprotection conditions for TAC base-protected oligonucleotides are 15 minutes at 55°C or 2 hours at room temperature, in a mixture of concentrated ammonia solution and ethanol (3/1, v/v). Alternatively, AMA reagent (concentrated ammonia/40% aqueous methylamine 1/1, v/v) can be employed for 30 minutes at room temperature. The shorter exposure time of the oligonucleotide to the alkaline deprotecting agent, compared to conventionally protected RNA oligonucleotides, reduces chain degradation and provides a higher yield of full length RNA product. Although Proligo Reagents’ TAC RNA monomers are stable for several days in solution, we recommend reconstitution of fresh amidites after 6 days on the instrument, to achieve optimal results. RNA monomers feature hydroxyl groups at the 2'position. In order to prevent the formation of unnatural 2'-5' phosphodiester bonds during chain elongation, the 2'OH group is protected with a trialkyl-silyl group, tert-butyldimethylsilyl (TBDMS). The TBDMS group is stable under the acidic conditions used to remove the DMT group during the synthesis cycle, but can be removed by a variety of methods after cleavage and deprotection of the RNA oligomer, e.g., with a solution of tetrabutylammonium fluoride (TBAF) in tetrahydrofan (THF) or with triethylamine hydrofluoride.

20

User Instructions
1. Use anhydrous acetonitrile (water content ≤30 ppm) as diluent. It is important to maintain anhydrous conditions while dissolving RNA amidites in acetonitrile. 2. For use on Expedite instruments, add 10 ml of acetonitrile to 0.5 g RNA monomer, to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 5 ml acetonitrile to 0.5 g RNA monomer to obtain a concentration of 100 mg/ml. 3. Gently swirl the vial until the powder is completely dissolved. 4. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 5. Once TAC RNA phosphoramidite has been dissolved and placed on your instrument, the phosphoramidite should be used within 6 days. 6. Enter the sequence of the RNA oligonucleotide you wish to synthesize. A minimum coupling time of 10 minutes or 6 minutes using Activator 42 is recommended for 2'-TBDMS-protected RNA amidites. 7. Fast deprotection Cap A must be employed in all RNA synthesis with the TAC-protected rG RNA phosphoramidite. 8. Proceed as you would with a standard DNA oligonucleotide synthesis. Depending on your intended further use of the oligomer, you can choose either DMT-On or DMT-Off procedures. The coupling efficiency of RNA monomers may be determined by standard dimethoxytrityl cation assays. 9. Cleave from the support and deprotect the RNA oligonucleotide with a mixture of concentrated ammonia and ethanol 3/1, v/v, at 55°C for 15 minutes, or at room temperature for 120 minutes. Alternatively, AMA reagent (concentrated ammonia/40% aqueous methylamine 1/1, v/v) can be employed for 10 minutes at 65°C. 10. It is essential to employ sterile conditions from this step forward. Always use sterilized water: preferably water recently treated with DEPC (diethyl pyrocarbonate, stir 1 L of HPLC grade water with 100 ml DEPC overnight and autoclave twice) and use baked glassware (250°C+ for more than 4 hours). 11. Transfer the supernatant solution of the RNA oligonucleotide into a separate vial. The yield of the RNA oligonucleotide can be improved by rinsing the support with ethanol/acetonitrile/ water 3/1/1, v/v, and combining the oligonucleotide solution with the washing solution. Evaporate to dryness. 12. Add a 1M solution of tetrabutylammonium fluoride (TBAF) in THF and incubate for 24 hours at room temperature. The deprotection time can be shortened to 6 hours if a TBAF-solution, with water content less than 5%, w/w, is employed. Following deprotection, add an equal volume of 1 M TEAA buffer pH 7, followed by another volume of water. Alternatively, deprotection can be accomplished using a mixture of neat triethylamine trihydrofluoride, triethylamine and N-methylpyrrolidon, 4/3/6, v/v, which can be employed for 90 minutes at 65°C. The deprotection reaction is quenched by the addition of an equal volume of water in this case. Note that the application of triethylamine trihydrofluoride in the DMT-On mode will lead to detritylation, due to the acidity of the reagent. 13. Desalt the RNA oligonucleotide by using a desalting matrix such as Sephadex® G25, an ion exchange cartridge, or a reversed phase purification cartridge. Optimal conditions for desalting vary greatly with the employed matrix/product. Conditions recommended by the manufacturer should generally be applied. The lyophilized crude RNA oligonucleotide product can be purified by AX-HPLC or by preparative gel electrophoresis.

21

Products

RNA Phosphoramidites, 2'O-Methyl RNA Phosphoramidites

2'O-Methyl RNA Phosphoramidites
OCH3

O HN N N N

2'O-Methyl RNA is a nucleic acid analog that is characterized by the exceptional hybridization properties that it imparts with complimentary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids. The unique combination of properties of 2'O-Methyl RNA had found widespread use in the fields of: • Diagnostic probes • Aptamer and ribozyme development • Mixed 2'O-Methyl-RNA/DNA antisense molecules 2'O-Methyl RNA nucleoside can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.
Chemical Formula: Formula Weight: Storage:
O N C O P OCH3 N(iPr)2 CH3O O O N

2’0-Methyl-rA(bz) Phosphoramidite
C48H54N7O8P 888.0 ≤+10°C

DMT-2'O-Methyl-rAdenosine(N6-Benzoyl)-ß-Cyanoethylphosphoramidite

O OMe N MeO O O O N HN

O N

OMe P O CN

Key features of 2'O-Methyl RNA Phosphoramidites
• High yield of crude oligonucleotides • Compatible with DNA synthesis • Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides • Recommended deprotection conditions are 8 hours at 55°C using concentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65°C • Purification and other downstream processing of fully modified 2'O-Methyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation • Synthesis of 2'O-Methyl RNA oligonucleotides is similar to standard DNA synthesis, but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers) • 2'O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry solutions.

2’O-Methyl-rC(ac) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C42H52N5O9P 801.9 ≤-10°C

DMT-2'O-Methyl-rCytidine(N4-acetyl)-ß-Cyanoethylphosphoramidite

OCH3 HN CH3O O O O

O

N

O N C O P

OCH3 N(iPr)2

2’0-Methyl-rU Phosphoramidite
Chemical Formula: Formula Weight: Storage: C40H49N4O9P 760.8 ≤+10°C

DMT-2'O-Methyl-rUridine-ß-Cyanoethylphosphoramidite

22

O OCH3 N CH3O O O O N HN O

OCH3 N N O

O NH N N H O

CH3O

O

O N C O P

OCH3 N(iPr)2

O N C O P

OCH3 N(iPr)2

2’0-Methyl-rC(tac) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C52H64N5O10P 950.1 ≤-10°C

2’0-Methyl-rG(ib) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C45H56N7O9P 870.0 ≤+10°C

DMT-2'O-Methyl-rCytidine(N4-tac)-ß-Cyanoethylphosphoramidite

DMT-2'O-Methyl-Guanosine(N2-Isobutyryl)-ß-Cyanoethylphosphoramidite

2’0-Methyl RNA Phosphoramidites
Catalog No. Description Unit Compatible with Expedite Instruments
A211181-01 A212181-01 C212181-01 C213181-01 G211181-01 G212181-01 U211181-01 DMT-2'O-Me-rA(bz) Amidite DMT-2'O-Me-rA(tac) Amidite DMT-2'O-Me-rC(tac) Amidite DMT-2'O-Me-rC(ac) Amidite DMT-2'O-Me-rG(ib) Amidite DMT-2'O-Me-rG(tac) Amidite DMT-2'O-Me-rU Amidite 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g

Customized packaging
Bulk packaging up to multiple kg per container and customized packaging with alternative quantities of amidites are available upon request.

Compatible with ABI Instruments
A211131-01 A212131-01 C212131-01 C213131-01 G211131-01 G212131-01 U211131-01 A211161-01 C212161-01 C213161-01 G211161-01 U211161-01 DMT-2'O-Me-rA(bz) Amidite DMT-2'O-Me-rA(tac) Amidite DMT-2'O-Me-rC(tac) Amidite DMT-2'O-Me-rC(ac) Amidite DMT-2'O-Me-rG(ib) Amidite DMT-2'O-Me-rG(tac) Amidite DMT-2'O-Me-rU Amidite DMT-2'O-Me-rA(bz) Amidite DMT-2'O-Me-rC(tac) Amidite DMT-2'O-Me-rC(ac) Amidite DMT-2'O-Me-rG(ib) Amidite DMT-2'O-Me-rU Amidite 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1x1g 1x1g 1x1g 1x1g 1x1g

Bulk Quantities
A211110-01 A212110-01 C212110-01 G211110-01 G212110-01 U211110-01 DMT-2'O-Me-rA(bz) Amidite DMT-2'O-Me-rA(tac) Amidite DMT-2'O-Me-rC(tac) Amidite DMT-2'O-Me-rG(ib) Amidite DMT-2'O-Me-rG(tac) Amidite DMT-2'O-Me-rU Amidite 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g

23

Products

RNA Phosphoramidites, 2'O-Methyl RNA Phosphoramidites

Final: Cleavage/Deprotection

1. Deblocking D MT O O Bas e HO O Bas e

2. Activation and Coupling

DMT O OC H 3 O OC H 3

O

O

Bas e

Solid Support

Solid Support N C

O O P

OC H 3 N(iPr)2

D MT

O

O

Bas e

Start next cycle: 1. Deblocking

D MT O N C O P O O O Bas e 4. Oxidation O OC H 3 N C OCH 3

O

O

Base

O O P O

OC H 3 O Bas e

Solid Support C H3

O O C O O 3. Capping Base

OC H 3

Solid Support

O

OC H 3

Solid Support

The Synthesis Cycle

2'O-Methyl RNA Synthesis
The synthesis cycle for 2'O-Methyloligoribonucleotides consists of the same series of reactions as the cycle that is employed for DNA monomers. However, the rate of coupling for 2'OMethyl RNA monomers is slower compared to that of DNA monomers (a coupling time of 6 minutes is recommended for 2'O-Methyl RNA monomers compared to 90 seconds for DNA monomers). With the exception of the 2'O-Methyl RNA monomers and supports, RNA synthesis is accomplished with the same reagents as DNA synthesis. All 2'O-Methyl RNA phosphoramidites from Proligo Reagents are diluted with dry acetonitrile.

Base Protection
Proligo Reagents’ 2'O-Methyl RNA monomers are compatible with fast deprotection schemes that are based on the application of aliphatic amines, such as methylamine. The adenosine and guanosine monomers are protected with the standard benzoyl and isobutyryl groups. The uridine monomer is unprotected at the base, and the cytidine monomer is protected with a TAC (tert-butylphenoxyacetyl) group. This protecting group avoids transamination side reactions at cytidines, when alkylamines are employed in the deprotection reaction. AMA reagent (concentrated ammonia/40% aqueous methylamine 1/1, v/v) can be conveniently applied.

2'O-Methyl RNA Supports 2'O-Methyl RNA Monomers
2'O-Methyl RNA monomers feature methoxy groups at the 2'-position. The methoxy groups are perfectly stable in all conditions employed in the assembly of oligonucleotides by automated phosphoramidite synthesis, and in all standard alkaline deprotection conditions. The supports for 2'O-Methyl oligoribonucleotide synthesis consist of a 2'O-Methyl RNA nucleoside covalently attached through the 3'-position to controlled pore glass (CPG). The pore size of Proligo Reagents’ CPG for 2'O-Methyl oligoribonucleotide synthesis is 500Å. Proligo Reagents offers ready-to-use synthesis columns for 2'O-Methyl oligoribonucleotide synthesis at 1 mol scale.

24

Methods
1. Use anhydrous acetonitrile (water content ≤30 ppm) as diluent. It is important to maintain anhydrous conditions while dissolving RNA phosphoramidites in acetonitrile. 2. For use on Expedite instruments, add 10 ml of acetonitrile to 0.5 g 2'O-Methyl RNA monomer, to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 5 ml acetonitrile to 0.5 g 2'O-Methyl RNA monomer, to obtain a concentration of 100 mg/ml. 3. Gently swirl the vial until the powder is completely dissolved. 4. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 5. Enter the sequence of the 2'O-Methyl oligoribonucleotide you wish to synthesize. A minimum coupling time of 6 minutes is recommended for 2'O-Methyl RNA phosphoramidites. 6. Proceed as you would with a standard DNA oligonucleotide synthesis. Depending on your intended further use of the oligomer, you can choose either DMT-On or DMT-Off procedures. The coupling efficiency of 2'OMethyl RNA monomers may be determined by standard dimethoxytrityl cation assays. 7. Cleave from the support and deprotect the 2'O-Methyl oligoribonucleotide with concentrated ammonia at 55°C for 8 hours. Alternatively, AMA reagent (concentrated ammonia/40% aqueous methylamine 1/1, v/v) can be employed for 10 minutes at 65°C. 8. The 2'O-Methyl oligoribonucleotide is now ready for further processing, such as desalting or purification with RP-HPLC, AXHPLC or gel-based methods. Purification of fully-modified 2'O-Methyl RNA oligonucleotides is simpler than in case of RNA, as no special precautions are required to prevent nucleolytic degradation

25

Products

DMT-2'Fluoro Phosphoramidites

DMT-2'Fluoro Phosphoramidites
2’Fluoro Phosphoramidites are used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.
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Analytical Specifications
Test HPLC Purity P-NMR 2'Fluoro-dC(ac) ≥98.0% ≥99% 2'Fluoro-dU ≥98.0% ≥99%

Features of 2'Fluoro Phosphoramidites:
• Can be employed together with DNA or RNA phosphoramidites • Recommended deprotection conditions are 8 hours at 55°C using concentrated ammonia solution, or with AMA for 10 minutes at 65°C • Synthesis of 2'Fluoro oligonucleotides is similar to standard DNA synthesis, but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers) • Consistent lot-to-lot high purity and performance • Manufactured under a certified ISO 9001 quality system

DMT -2'Fluoro Phosphoramidites
Catalog No.
C213281-01 C213231-01 C213233-01 U211281-01 U211231-01 U211233-01

Description
DMT-2'Fluoro-dC(ac) Amidite, 89 DMT-2'Fluoro-dC(ac) Amidite, ABI DMT-2'Fluoro-dC(ac) Amidite, ABI DMT-2'Fluoro-dU Amidite, 89 DMT-2'Fluoro-dU Amidite, ABI DMT-2'Fluoro-dU Amidite, ABI

Unit
1 x 0.25 g 1 x 0.25 g 1x1g 1 x 0.25 g 1 x 0.25 g 1x1g

Please ask for delivery dates of these specialty amidites.

O OMe HN N MeO O O O O P O N CN F N

OMe HN MeO O O O O P O N F

O

N

CN
DMT -2'Fluoro-dU Amidite

DMT -2'Fluoro-dC(ac) Amidite

26

Labels and Modifications

Labels and Modifications
Phosphoramidite technology is ideally suited for the modification of synthetic oligonucleotides with covalently attached reporter moieties, linkers, spacers or haptens. SAFC Supply Solutions provides popular reporters and linkers ready to use for DNA/RNA synthesis machines such as ß-cyanoethyl phosphoramidites.

Fluorescein Phosphoramidite
Fluorescein phosphoramidite is composed of a protected fluorescein molecule linked to a ß-cyanoethylphosphoramidite. The fluorescein derivative consists of two isomers derived from 5and 6-carboxy fluorescein. As a result, two peaks will be seen on a RP-HPLC chromatogram of the synthesis product.

Biotin Phosphoramidite
Biotin-labeled oligonucleotides have become extremely popular in DNA capture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotin-based detection methods and are widely used by researchers. Biotin labeling is compatible with PCR and most hybridization techniques.

Key Features of the Fluorescein Phosphoramidite
• Readily soluble in acetonitrile • Couples to the 5'-end of the oligonucleotide using standard synthesis protocols • Protecting groups on the fluorescein moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia • A detritylation step is not required since the fluorescein phosphoramidite does not contain a dimethoxytrityl(DMT-) group • The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required • The fluorescein moiety provides a purification handle in RP-HPLC purification

Key Features of the Biotin Phosphoramidite
• Behaves like any standard amidite on the DNA synthesizer • Readily soluble in acetonitrile • Biotin compound has a dimethoxytrityl (DMT) group on the ring structure, which allows coupling-yield determination and trityl-selective purification • A long, water-compatible linker arm ensures maximum sensitivity • Provides a coupling efficiency of at least 95%

27

Products

Labels and Modifications

Amino Linkers
Amino linkers can be employed to conjugate biotin, fluorescein or other modifiers and reporter groups to the 5'end of oligonucleotides, or to attach oligonucleotides to surfaces. SAFC Supply Solutions offers 2 monomethoxytrityl-protected amino linkers (MMT-linkers) and 2 trifluoroacetyl protected amino linkers (TFA linkers): • Trifluoroacetyl (TFA)-protected pentyl (C5) amino linker • Trifluoroacetyl (TFA)-protected hexyl (C6) amino linker • Monomethoxytrityl (MMT)-protected amino linker • ssH-linker: the next generation of MMT linker

The MMT-group of ssH-linker serves as an excellent purification handle after the oligonucleotide synthesis in trityl-on mode, similar to the DMT-group of conventional oglionucleotides. The MMT-group is cleaved under very mild conditions in aqueous acetic acid (10% glacial acetic acid in water, 20 min. room temp.) Alternatively, the MMT-group can be cleaved on the synthesis instrument with acidic deblock solution to enable on-support labeling protocols.

Key features of amino linkers
• Completely soluble in acetonitrile • Base-labile TFA- as well as acid-labile MMTprotecting groups on the amino linker • Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites • No change in auxiliary synthesis reagents is required • The MMT-amino linker features a lipophilic group which aids in purification of the modified oligonucleotide after synthesis • The acid-labile MMT -group permits the colorimetric determination of the coupling efficiency • It is recommended to deprotect MMT-amino linker oligonucleotides in concentrated ammonia at a lower temperature e.g., at 40°C for 24 hours • The MMT-group can be removed from the oligonucleotide at room temperature - in case of MMT-amino linker with 80% aqueous acetic acid in 3 hours - in case of ssH-linker with 10% aqueous acetic acid in 20 minutes • TFA-amino linker is completely deprotected with concentrated ammonia. Additional deprotection steps are not necessary

Monomethoxytrityl (MMT)-protected amino linker
The MMT-group can be cleaved on the synthesis instrument with acidic deblock solution to enable on-support labeling protocols. Alternatively, the MMT-amino linker can be attached in the trityl-on mode of the instrument to provide purification handle similar to the DMT-group of the conventional oligonucleotides. The MMT-group is then removed with aqueous acid after purification with either RP-HPLC or a purification cartridge.

Trifluoroacetyl (TFA)-protected amino linker
The base-labile TFA-group is easily removed with concentrated ammonia during the cleavage and deprotection step. Additional deprotection steps are not necessary.

ssH-linker
ssH-linker comprises an internal carbamate group, which is attached to the MMT-protected amino group via a short spacer. The carbamate molety facilitates the cleavage of the MMT-group under mildly acidic conditions while increasing the stability of the MMT-group during the deprotection of the oligonucleotide with ammonia. The carbamate group also enhances the reactivity of the amino group through a neighbor group effect, and thereby accelerates conjugations to aminoreactive modifiers and reporters.

28

Key features of ssH-linker:
• Advantages over conventional amino linkers - Deprotection of the amino-group under exceptionally mild conditions which avoid depurination side reaction - Deprotection with 10% aqueous acetic acid at ambient temperature for 20 minutes - Better labeling efficiency than C6-amino linkers - Higher coupling efficiency than C6-amino linkers - Better trityl-on purification efficiency • General features - Excellent coupling efficiency

Labels and Modifications
Compatible with Expedite and Polygen Instruments Catalog No.
M010982-01 M010882-01 M010181-01 M010282-01 M010381-01 M010382-01 M010682-01

Description
ssH-Linker TFA Hexylaminolinker Fluorescein Amidite MMT-Hexylamine-Linker Amidite Biotin Amidite Biotin Amidite TFA Pentylaminolinker Amidite

Unit
1 x 0.25 g 1 x 0.25 g 1 x 0.1 g 1 x 0.25 g 1 x 0.1 g 1 x 0.25 g 1 x 0.25 g

Compatible with ABI Instruments
M010932-01 M010832-01 M010131-01 ssH-Linker TFA Hexylaminolinker Amidite Fluorescein Amidite MMT-Hexylamine-Linker Amidite Biotin Amidite Biotin Amidite TFA Pentylaminolinker Amidite 1 x 0.25 g 1 x 0.25 g 1 x 0.1 g 1 x 0.25 g 1 x 0.1 g 1 x 0.25 g 1 x 0.25 g

- Used with standard deblock, activator, oxidizer and capping-solutions

M010232-01 M010331-01 M010332-01 M010632-01

Custom products
Phosphoramidites of non-catalog linkers, reporters or modifiers are offered as custom synthesis products.

Fluorescein Phosphoramidite

Biotin Phosphoramidite

MMT -Amino Linker

TFA Pentyl Amino Linker

TFA Hexyl Amino Linker

ssH-Linker

29

Products

Labels and Modifications

Fluorescein Phosphoramidite
Chemical Formula: Formula Weight: Storage: C46H58N3O10P 843.9 ≤-10°C

Fluorescein-ß-Cyanoethylphosphoramidite

Method
1. Use anhydrous acetonitrile (water content ≤30 ppm) to dissolve the fluorescein phosphoramidite. It is important to maintain anhydrous conditions when dissolving the fluorescein phosphoramidite in acetonitrile. 2. For use on Expedite instruments, add 2 ml acetonitrile to 0.1 g fluorescein phosphoramidite (M010181-01) to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 1.2 ml acetonitrile to 0.1 g fluorescein phosphoramidite (M010131-01) to prepare a 0.1 M solution. 3. Gently swirl the vial until the powder is completely dissolved. 4. Once the fluorescein phosphoramidite has been dissolved and placed on your instrument, it should be used within 4 days. If you do not plan to use all of the material in 4 days, remove the vial, seal carefully and store at – 20°C until needed. 5. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 6. Enter the sequence of the oligonucleotide you wish to synthesize with fluorescein phosphoramidite at the 5'-end. A minimal coupling time of 3 minutes is recommended for fluorescein phosphoramidite. 7. Proceed as you would with a standard DNA oligonucleotide synthesis. Note that fluorescein phosphoramidite from Proligo Reagents does not contain a DMT group. Oligonucleotides do not need to be detritylated at the end of the synthesis. Note that the fluorescein phosphoramidite will terminate the synthesis and can only be employed in the last coupling step on the 5' terminus. 8. Cleave and deprotect the oligonucleotide with ammonia at 55°C for 8 hours with standard protected nucleobases, or, if TAC-protected phosphoramidites are used, at 55°C for 15 minutes. The fluorescein-moiety is stable under these conditions. 9. The oligonucleotide is now ready for further processing, such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods. The fluorescein label allows the purified fraction to be easily detected during collection. 10. Oligonucleotides labeled with fluorescein should be stored in the dark.

30

Biotin Phosphoramidite
Chemical Formula Formula Weight: Storage: C46H64N5O8PS 878.1 ≤-10°C

DMT-Biotin-ß-Cyanoethylphosphoramidite

Method
1. Use anhydrous acetonitrile (water content ≤30 ppm) to dissolve the biotin phosphoramidite. It is important to maintain anhydrous conditions when dissolving the biotin phosphoramidite in acetonitrile. 2. For use on Expedite instruments, add 2 ml acetonitrile to 0.1 g biotin phosphoramidite (M010381-01) or 5ml acetonitrile to 0.25 g biotin phosphoramidite (M010382-01) to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 1ml acetonitrile to 0.1 g biotin phosphoramidite (M010331-01), or, to obtain a concentration of 100 mg/ml, add 2.5 ml acetonitrile to 0.25 g biotin phosphoramidite (M010332-01). 3. Gently swirl the vial until the powder is completely dissolved. 4. Once the biotin phosphoramidite has been dissolved and placed on your instrument, it should be used within 48 hours. If you do not plan to use all of the material in 48 hours, remove the vial, seal carefully and store at –20°C until needed. 5. Biotin phosphoramidite is supplied in V-vials for the Expedite instrument. A new end-line filter should be installed prior to placing the phosphoramidite on a Expedite instrument. The tubing length can be shortened by cutting the tubing to fit the V-vial. 6. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 7. Enter the sequence of the oligonucleotide you wish to synthesize with biotin phosphoramidite at the 5'-end. The coupling time for biotin phosphoramidite is the same as that recommended by the instrument manufacturer for the four standard DNA phosphoramidites A, C, G and T. Note that the biotin phosphoramidite will terminate the synthesis and can only be employed in the last coupling step on the 5' terminus. 8. Proceed as you would with a standard DNA oligonucleotide synthesis. Depending on your intended further usage of the oligomer, you can either choose DMT-On, or, DMT-Off procedures. The coupling efficiency of the biotin phosphoramidite may be determined by a standard dimethoxytrityl cation assay. 9. We recommend to elongate the last acidic deblocking step, for the release of the DMTgroup on the biotin moiety, in DMT-Off mode. A deprotection time of 5 minutes is sufficient. 10. Cleave and deprotect the oligonucleotide with ammonia at 55°C for 8 hours with standard protected nucleobases, or, if TAC-protected phosphoramidites are used, at 55°C for 15 minutes. Biotin phosphoramidite from Proligo Reagents is compatible with standard and fast deprotection schemes. The biotin moiety maintains its biological activity after it is processed using standard workup conditions. 11. The oligonucleotide is now ready for further processing, such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods.

31

Products

Labels and Modifications

ssH-Linker
Chemical Formula: Formula Weight : Storage : C38H53N4O5P 676,83 ≤-10°C

Method
1. Use anhydrous acetonitrile (water content 30 ppm) to dissolve the ssH-linker.* It is important to maintain anhydrous conditions during liquid transfer and dissolution. 2. For use on Expedite instruments, add 5ml acetonitrile to 0.25 g ssH-linker (M010982-01) to obtain a concentration of 50 mg/ml. For use on ABI® instruments, add 3.7 ml acetonitrile to 0.25 g ssH-linker (M010932-01) to prepare a 0.1 M solution. 3. The ssH-linker is a viscous oil that requires more time to dissolve than powdered phosphoramidites. Gently swirl the vial until the linker is completely dissolved. 4. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 5. Enter the sequence of the oligonucleotide you wish to synthesize with ssH-linker. The coupling time for ssH-linker is the same as that recommended by the instrument manufacturer for the four standard DNA phosphoramidites A, C, G and T. Note that the ssH-linker will terminate the synthesis and can only be employed in the last coupling step on the 5' terminus. 6. Proceed as you would with a standard DNA oligonucleotide synthesis. Depending on your intended further usage of the oligomer, you can either choose Trityl-On or Trityl-Off procedures. The coupling efficiency of the ssH-linker may be determined by a monomethoxytrityl cation assay in Trityl-Off mode. Standard deblock steps as used for the removal of DMT-groups during oligonucleotide chain assembly can be applied for the removal of the MMT*-group in Trityl-Off mode. 7. After synthesis in Trityl-Off mode the oligonucleotide is ready for on-support labeling. Perform the labeling reaction by incubating the support in the respective reaction mixture and wash the support appropriately.** 8. Cleave and deprotect the oligonucleotide with ammonia at 40°C for 24 hours with standard protected nucleobases, or, if TAC-protected phosphoramidites are used, at 55°C for 15 minutes. 9. The oligonucleotide is now ready for further processing, such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods. MMT-protected ssH-linker oligonucleosides are particularly suitable for cartridge-based reverse phase purification. 10. Oligonucleotides prepared in Trityl-On mode are further deprotected by a treatment with 10% aqueous acetic acid for 20 minutes at room temperature. Acetic acid is removed by evaporation under vacuum. Free MMT residues can be removed, if desired, by extraction of an aqueous solution of the oligonucleotide with diethyl ether.
* monomethoxytrityl ** During oligonucleotide deprotection in ammonia the amino group should either be MMT-protected or conjugated to a reporter group. Unprotected amino groups will react with the internal carbamate linkage under deprotection conditions resulting in a derivative which is unreactive to common labeling reagents.

32

MMT -Amino Linker Phosphoramidite
Chemical Formula: Formula Weight: Storage: C35H48N3O3P 589.8 ≤-10°C

MMT-Aminohexanol-ß-Cyanoethylphosphoramidite

Method
1. Use anhydrous acetonitrile (water content ≤30 ppm) to dissolve the MMT-amino linker phosphoramidite. It is important to maintain anhydrous conditions when dissolving the linker compound in acetonitrile. 2. For use on Expedite instruments, add 5ml acetonitrile to 0.25 g MMT-amino linker phosphoramidite (M010282-01) to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 4.2 ml acetonitrile to 0.25 g MMT-amino linker phosphoramidite (M010232-01) to prepare a 0.1 M solution. 3. The MMT -amino linker phosphoramidite is a viscous oil that requires more time to dissolve than powdered phosphoramidites. Gently swirl the vial until the linker is completely dissolved. 4. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 5. Enter the sequence of the oligonucleotide you wish to synthesize with MMT -amino linker phosphoramidite. The coupling time for MMT amino linker phosphoramidite is the same as that recommended by the instrument manufacturer for the four standard DNA phosphoramidites A, C, G and T Note that the . MMT -amino linker phosphoramidite will terminate the synthesis and can only be employed in the last coupling step on the 5' terminus. 6. Proceed as you would with a standard DNA oligonucleotide synthesis. Depending on your intended further usage of the oligomer, you can either choose Trityl-On, or, Trityl-Off procedures. The coupling efficiency of the MMT-amino linker phosphoramidite may be determined by a monomethoxytrityl cation assay in Trityl-Off mode. 7. We recommend to elongate the last acidic deblocking step, for the release of the MMTgroup on the amino linker, in Trityl-Off mode. A deprotection time of 5 minutes is sufficient. 8. Upon synthesis in Trityl-Off mode, treat the CPG-bound oligonucleotide with an excess of a 10% solution of triethylamine in acetonitrile for 10 minutes at room temperature and wash with acetonitrile. This procedure cleaves the cyanoethyl-protective groups from the phosphate moieties of the oligonucleotide and prevents side-reactions arising from the alkylation of the primary amine. 9. Cleave and deprotect the oligonucleotide with ammonia at 40°C for 24 hours with standard protected nucleobases, or, if TACprotected phosphoramidites are used, at 55°C for 15 minutes. 10. The oligonucleotide is now ready for further processing, such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods. Cartridge-based reverse phase methods are suitable for oligonucleotides prepared with the MMT-amino linker phosphoramidite in Trityl-On mode. 11. Oligonucleotides prepared in Trityl-On mode are further deprotected by a treatment with 80% acetic acid for 3 hours at room temperature. Acetic acid is removed by vacuum centrifugation. Free MMT residues can be removed, if desired, by extraction of an aqueous solution of the oligonucleotide with diethyl ether.

33

Products

Labels and Modifications

TFA-Pentylamino Linker Phosphoramidite
Chemical Formula: Formula Weight: Storage: C16H29F3N3O3P 399.4 ≤-20°C

TFA-Hexylamino Linker Phosphoramidite
Chemical Formula: Formula Weight: Storage: C17H31F3N3O3P 413.4 ≤-20°C

Trifluoroacetyl-Aminopentanol-ß-Cyanoethylphosphoramidite

Trifluoroacetyl-Aminohexanol-ß-Cyanoethylphosphoramidite

Method
1. Use anhydrous acetonitrile (water content ≤30 ppm) to dissolve the TFA-amino linker phosphoramidite. It is important to maintain anhydrous conditions when dissolving the linker compound in acetonitrile. 2. For use on Expedite instruments, add 5 ml acetonitrile to 0.25 g TFA-amino linker phosphoramidite (M010682-01) to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 6.3ml acetonitrile to 0.25 g TFA-amino linker phosphoramidite (M01063201) to prepare a 0.1 M solution. 3. The TFA-amino linker phosphoramidite is a viscous oil that requires more time to dissolve than powdered phosphoramidites. Gently swirl the vial until the linker is completely dissolved. 4. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 5. Enter the sequence of the oligonucleotide you wish to synthesize with the TFA-amino linker phosphoramidite. The coupling time for the TFA-amino linker phosphoramidite is the same as that recommended by the instrument manufacturer for the four standard DNA phosphoramidites A, C, G and T Note that the . TFA-amino linker phosphoramidite will terminate the synthesis and can only be employed in the last coupling step on the 5' terminus. 6. Proceed as you would with a standard DNA oligonucleotide synthesis using the Trityl-OFF mode. 7. Treat the CPG-bound oligonucleotide with an excess of a 10% solution of triethylamine in acetonitrile for 10 minutes at room temperature and wash with acetonitrile. This procedure cleaves the cyanoethyl-protective groups from the phosphate moieties of the oligonucleotide and prevents side-reactions arising from the alkylation of the primary amine. 8. Cleave and deprotect the oligonucleotide with ammonia at 55°C for 8 hours with standard protected nucleobases. If the conventional isobutyryl (ib) protective group on dG is replaced with the dimethylformamidine (dmf) group, a shorter deprotection time of 2 hours at 55°C may be used. 9. The oligonucleotide is now ready for further processing, such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods. Note that cartridge-based reverse phase methods are not suitable for oligonucleotides prepared with the TFA-amino linker phosphoramidite.

34

Non-Standard Nucleosides

Non-Standard Nucleosides
Several applications of synthetic oligonucleotides are based on nucleosides with alternative heterocycles. SAFC Supply Solutions provides

Non-Standard Nucleosides
Compatible with Expedite and Polygen Instruments Catalog No.
M113381-01 M111181-01

Description
5-Methyl-dC(ac) Amidite DMT-dInosine Amidite DMT-dUridine Amidite

Unit
1 x 0.25 g 1 x 0.25 g 1 x 0.25 g

phosphoramidites of selected non-standard
M110281-01

nucleosides as catalog items and offers custom synthesis services for proprietary monomers.

Compatible with ABI Instruments
M113331-01 M111131-01 M110231-01 5-Methyl-dC(ac) Amidite DMT-dInosine Amidite DMT-dUridine Amidite 1 x 0.25 g 1 x 0.25 g 1 x 0.25 g

OCH3 HN CH3O O O O

O

Method
1. Use anhydrous acetonitrile (water content ≤30 ppm) as diluent. It is important to maintain anhydrous conditions while dissolving deoxyuridine phosphoramidite in acetonitrile. 2. For use on Expedite instruments, add 5ml acetonitrile to 0.25 g deoxyuridine phosphoramidite (M110281-01) to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 3.4 ml acetonitrile to 0.25 g deoxyuridine phosphoramidite (M110231-01) to prepare a 0.1 M solution. 3. Gently swirl the vial until the powder is completely dissolved. 4. Attach the dissolved phosphoramidite to an appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 5. Enter the sequence of the oligonucleotide you wish to synthesize with deoxyuridine phosphoramidite. The coupling time for deoxyuridine phosphoramidite is the same as that recommended by the instrument manufacturer for the four standard DNA phosphoramidites A, C, G and T. 6. Proceed as you would with a standard DNA oligonucleotide synthesis. 7. Cleave and deprotect the oligonucleotide with ammonia at 55°C for 8 hours with standard protected nucleobases, or, if TAC-protected amidites are applied, at 55°C for 15 minutes. Deoxyuridine phosphoramidite from Proligo Reagents is compatible with standard and fast deprotection schemes, including methylamine-derived deprotection agents such as AMA. 8. The oligonucleotide is now ready for further processing such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods.

N

O N C O P N

Deoxyuridine Phosphoramidite
Chemical Formula: Formula Weight: Storage: C39H47N4O8P 730.8 ≤+10°C

DMT-Deoxyuridine-ß-Cyanoethylphosphoramidite

35

Products

Non-Standard Nucleosides

OCH3 N N O

O NH N

CH3O

O

O N C O P N

Deoxyinosine Phosphoramidite
Chemical Formula: Formula Weight: Storage: C40H47N6O7P 754.8 ≤+10°C

DMT-Deoxyinosine-ß-Cyanoethylphosphoramidite

Method
1. Use anhydrous acetonitrile (water content ≤30 ppm) to dissolve the deoxyinosine phosphoramidite. It is important to maintain anhydrous conditions when dissolving the deoxyinosine phosphoramidite in acetonitrile. 2. For use on Expedite instruments, add 5 ml acetonitrile to 0.25 g deoxyinosine phosphoramidite (M111181-01) to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 3.3 ml acetonitrile to 0.25 g deoxyinosine phosphoramidite (M111131-01) to prepare a 0.1 M solution. 3. Gently swirl the vial until the powder is completely dissolved. 4. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 5. Enter the sequence of the oligonucleotide you wish to synthesize with deoxyinosine phosphoramidite. The coupling time for deoxyinosine phosphoramidite is the same as that recommended by the instrument manufacturer for the four standard DNA phosphoramidites A, C, G and T. 6. Proceed as you would with a standard DNA oligonucleotide synthesis. 7. For oligonucleotides with a high content of deoxyinosine, we recommend to treat the CPG-bound oligonucleotide with an excess of a 10% solution of triethylamine in acetonitrile for 10 minutes at room temperature, followed by washing with acetonitrile. This procedure cleaves the cyanoethyl-protecting groups from the phosphate moieties of the oligonucleotide and prevents minor sidereactions arising from the alkylation of the base of deoxyinosine phosphoramidite. 8. Otherwise, cleave and deprotect the oligonucleotide with ammonia at 55°C for 8 hours with standard protected nucleobases, or, if TAC-protected phosphoramidites are used, at 55°C for 15 minutes. Our Deoxyinosine phosphoramidite is compatible with standard and fast deprotection chemistries, including methylamine-derived deprotection agents such as AMA. 9. The oligonucleotide is now ready for further processing, such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods.

36

O OCH3 N CH3 O O O O N HN CH3 CH3

O N C O P N

5-Methyl-dC(ac) Phosphoramidite
Chemical Formula: Formula Weight: Storage: C42H52N5O8P 785.9 ≤-20°C

DMT-5-Methyl-Deoxycytidine(ac)-ß-Cyanoethylphosphoramidite

Method
1. Use anhydrous acetonitrile (water content ≤30 ppm) to dissolve the 5-methyl-dC(ac) phosphoramidite. It is important to maintain anhydrous conditions when dissolving the 5methyl-dC(ac) phosphoramidite in acetonitrile. 2. For use on Expedite instruments, add 5 ml acetonitrile to 0.25 g 5-methyl-dC(ac) phosphoramidite (M113381-01) to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 3.2 ml acetonitrile to 0.25 g 5-methyl-dC(ac) phosphoramidite (M113331-01) to prepare a 0.1 M solution. 3. Gently swirl the vial until the powder is completely dissolved. 4. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 5. Enter the sequence of the oligonucleotide you wish to synthesize with 5-methyl-dC(ac) phosphoramidite. The coupling time for 5-methyldC(ac) phosphoramidite is the same as that recommended by the instrument manufacturer for the four standard DNA phosphoramidites A, C, G and T. 6. Proceed as you would with a standard DNA oligonucleotide synthesis. 7. Cleave and deprotect the oligonucleotide with ammonia at 55°C for 8 hours with standard protected nucleobases, or, if TAC-protected phosphoramidites are used, at 55°C for 15 minutes. 5-methyl-dC(ac) phosphoramidite from Proligo Reagents is compatible with standard and fast deprotection chemistries, including methylamine-derived deprotection agents such as AMA. 8. The oligonucleotide is now ready for further processing, such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods.

37

Products

Phosphate-ON Phosphoramidite

Phosphate-ON Phosphoramidite
Phosphate-ON phosphoramidite is a chemical phosphorylation reagent that can be employed to introduce a phosphate group at the 5' terminus of an oligonucleotide. Chemical phosphorylation is a cost-effective alternative to enzymatic phosphorylation methods, allowing introduction of terminal phosphate groups in high yields, at any desired scale.

Key Features of Phosphate-ON Phosphoramidite
• Fully compatible with standard phosphoramidite reagents and synthesis conditions • Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotide can be cleaved from the support and deprotected in concentrated ammonia at 55°C in only 2 hours • Comprises a DMT-group for trityl monitoring • Dissolves easily in acetonitrile to a 0.1 M solution

SAFC Supply Solutions’ Proligo® Reagents offers Phosphate-ON phosphoramidite in powder form, enabling the dissolution of the reagent in acetonitrile to be easily monitored. The application of the reagent is fully compatible with standard cleavage and deprotection procedures at the end of oligonucleotide synthesis. Our phosphate-ON phosphoramidite comprises a thymidine nucleoside, which is removed from the oligonucleotide during the cleavage and deprotection process.

• Powder format enables dissolution to be easily monitored and facilitates product handling • High coupling efficiency of ≥98.0% under standard DNA phosphoramidite coupling conditions • Cleavage of both phosphate protective groups through a ß-elimination process

Key Features of 5'-Phosphorylated Oligonucleotides
• Enables enzymatic ligation to the 3'-terminus of nucleic acids • Offers protection against exonucleolytic degradation • Provides oligonucleotide conjugation through activation with coupling reagents such as EDC These features enable 5'-phosphorylated oligonucleotides to be used in applications such as molecular cloning and gene construction; ligase chain reaction; template directed ligation; oligonucleotide labeling with reporter groups, haptens and other modifiers; and oligonucleotide stabilization.

Phosphate-ON Phosphoramidite
Compatible with Expedite and Polygen Instruments Catalog No.
M010782-01

Description
Phosphate-ON Amidite

Unit
1 x 0.25 g

Compatible with ABI Instruments
M010732-01 Phosphate-ON Amidite 1 x 0.25 g

Other quantities available upon request.

38

Phosphate-ON Phosphoramidite
Chemical Formula: Formula Weight: Storage: C49H62N7O11P 956.0 ≤-10°C

Method
1. Use anhydrous acetonitrile (water content ≤30 ppm) as diluent. It is important to maintain anhydrous conditions while dissolving phosphate-ON phosphoramidite in acetonitrile. 2. For use on Expedite instruments, add 5 ml acetonitrile to 0.25 g phosphate-ON phosphoramidite (M010782-01) to obtain a concentration of 50 mg/ml. For use on ABI instruments, add 2.5 ml acetonitrile to 0.25 g phosphate-ON phosphoramidite (M010732-01) to obtain a concentration of 100 mg/ml. 3. Gently swirl the vial until the powder is completely dissolved. 4. Once the phosphate-ON phosphoramidite has been dissolved and placed on your instrument, it should be used within 4 days. If you do not plan to use all of the material in 4 days, remove the vial, seal carefully, and store at –20°C until needed. 5. Attach the dissolved phosphoramidite to the appropriate position on the synthesizer. Ensure that the delivery line to the synthesis chamber is sufficiently primed. 6. Enter the sequence of the oligonucleotide you wish to synthesize with a phosphate group at the 5'-end. The coupling time for phosphate-ON phosphoramidite is the same as that recommended by the instrument manufacturer for the four standard DNA phosphoramidites A, C, G and T. 7. The phosphate-ON phosphoramidite is designed for the phosphorylation of the 5'-end of the oligonucleotide and should only be employed in the last coupling step. 8. Proceed as you would with a standard DNA oligonucleotide synthesis. Depending on your further use of the oligomer, you can either choose DMT-On or DMT-Off procedures. The coupling efficiency of the phosphate-ON phosphoramidite may be determined by a standard dimethoxytrityl cation assay. 9. Cleave and deprotect the oligonucleotide with ammonia at 55°C for a minimum time of 2 hours. Our phosphate-ON phosphoramidite is compatible with a variety of deprotection conditions and base-protection schemes, including standard protective groups (ammonia for 8 hours at 55°C), the incorporation of dimethylformamidine (dmf) protected guanidine monomers (ammonia for 2 hours at 55°C), and the incorporation of tert-butylphenoxyacetyl (TAC) protected cytidine monomers (AMA reagent for 10 minutes at 65°C). If the DMT -On modus is used, the DMT group will be removed during the final cleavage and deprotection step. 10. The oligonucleotide is now ready for further processing such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods.

39

Products

NPPOC Phosphoramidites

NPPOC Phosphoramidites
Phosphoramidites with a NPPOC 5'-protection group can be used for the production of DNA

NPPOC Phosphoramidites
Catalog No.
A112N01-01 C114N01-01

Description
NPPOC-dA(tac) Amidite NPPOC-dC(ib) Amidite NPPOC-dG(ipac) Amidite NPPOC-dT Amidite

Unit
1x1g 1x1g 1x1g 1x1g

microarrays using photolithographic in situ synthesis of oligonucleotides directly on the chip. This method offers the possibility to

G114N01-01 T111N01-01

Please ask for delivery dates of these specialty amidites.

produce DNA chips of extremely high density and provides a flexible system.

Features of NPPOC Chemistry:
• Efficient photolytic deprotection (> 99%) • Less side products during photolysis • Products available • Lot-to-lot consistent high purity and performance • Manufactured under a certified ISO 9001 quality system

Analytical Specifications
Test
HPLC Purity
31

A
≥96.0% ≥96%

C
≥96.0% ≥96%

G
≥96.0% ≥96%

T
≥96.0% ≥96%

P-NMR

O HN O O NO2 O P O N CN O O N N N
NO2 O P O N

O

O N
O O O O O

HN N N

CN

NPPOC-dA(tac) Amidite

NPPOC-dC(ib) Amidite

O O O NO2 O O O P O N N N N NH N H O O

O O O NO2 O O HN O O N

CN

P O N CN

NPPOC-dG(ipac) Amidite

NPPOC-dT Amidite

40

User Instructions
1. Liquid Reagents
Deblock — The NPPOC-protective group is removed with light while the support-anchored oligonucleotide is immersed in a liquid reagent; various formulations are applicable, our recommended formulations are 1% NMI in DMSO (v/v) or 1% NMI in ACN (v/v) Activator — Any commercial activator that works for DNA-amidites is applicable; 0.25 M DCI in ACN is perfect Oxidizer — Although any commercial oxidizer is applicable some customers use flow cells that are incompatible with THF and therefore prefer to use an oxidizer wherein the solvent THF is replaced by ACN Cap A — Fast Cap A is recommended (TACanhydride solution); some customers use flow cells that are incompatible with THF and therefore prefer to use a Cap A reagent wherein the solvent THF is replaced by ACN; some customers do not employ capping steps with NPPOC-chemistry Cap B — Cap B solutions that are compatible with Fast Cap A; some customers use flow cells that are incompatible with THF and therefore prefer to use a Cap B reagent wherein the solvent THF is replaced by ACN

2. Coupling
NPPOC-amidites are dissolved in ACN at the concentration recommended for standard DNA amidites by the manufacturer of the DNA/RNA synthesizer. The coupling times of NPPOCAmidites match the coupling times of standard DNA amidites (30 to 60 sec., longer coupling times are applicable, but not necessary).

3. Deprotection of NPPOC-groups
Light with a wavelength between 350 and 390 nm is applicable. The deprotection time depends on the light intensity. 90 sec. illumination time is sufficient at 100 mW/cm2 on the surface of the synthesis support (365 nm Hg/Xe high pressure lamp).

4. Deprotection of base protective groups
Any fast deprotection protocol (e.g., conc. ammonia/room temp./2 hours or AMA/room temp./30 min.) is applicable. However, such treatment will partly remove the oligonucleotides from silica surfaces and therefore a milder treatment with a mixture of ethylendiamine and ethanol 50/50, v/v, for 2 hours at room temperature is used as an industry standard.

41

Products

Controlled Pore Glass and Columns — CPG Free Flow

Controlled Pore Glass
Since Professor Merrifield first described solid phase synthesis, researchers have investigated numerous supports for the chemical synthesis of DNA. Controlled Pore Glass (CPG) has proven to be the most accepted standard, with features such as high surface area, efficient mass transfer and chemical inertness. Because the pore density of CPG can be adjusted, the solid support can be tailored to the size of the synthetic oligonucleotide required. Bigger pore size is typically preferable in minimizing steric effects during the synthesis of long oligonucleotides, while smaller pore size fosters improved synthesis consistency. Our process guarantees consistent production of CPGs to specifications designed for DNA synthesis. All products are tested in our Quality Control labs for purity and identity prior to release.

Analytical Parameters of CPG
CPG (UHL)
Pore size (nm) Pore volume (ml/g) Grain size ( m) Surface (m2/g) 33 1.3 100–180 ~100

CPG 500Å
50 1.0 100–180 ~60

CPG 1000Å
100 1.3 100–180 ~30

Nucleoside-Loaded CPG
Loading mol/g
CPG 500Å CPG 1000Å CPG UHL CPG UHL 30–40 25–35 50–70 90–110

Fast Deprotection CPG
Catalog No.
C303001-01 C303010-01 C403001-01 C403010-01

Description
CPG 500Å dC(ac) 30-40 mol/g CPG 500Å dC(ac) 30-40 mol/g CPG 1000Å dC(ac) 25-35 mol/g CPG 1000Å dC(ac) 25-35 mol/g CPG 500Å dA(tac), 30-40 mol/g CPG 500Å dC(tac), 30-40 mol/g CPG 500Å dG(tac), 30-40 mol/g CPG 1000Å dA(tac), 25-35 mol/g CPG 1000Å dC(tac), 25-35 mol/g CPG 1000Å dG(tac), 25-35 mol/g CPG 500Å dC(tac), 30-40 mol/g CPG 1000Å dC(tac), 25-35 mol/g CPG 500Å dG(dmf), 30-40 mol/g CPG 500Å dG(dmf), 30-40 mol/g

Unit
1x1g 1 x 10 g 1x1g 1 x 10 g 1x1g 1x1g 1x1g 1x1g 1x1g 1x1g 1 x 10 g 1 x 10 g 1x1g 1 x 10 g

DNA CPG
Catalog No.
A301001-01 C301001-01 G301001-01 T301001-01 A301010-01 C301010-01 G301010-01 T301010-01 A401001-01 C401001-01 G401001-01 T401001-01 A401010-01 C401010-01 G401010-01 T401010-01

A302001-01

Description
CPG 500Å dA(bz), 30-40 mol/g CPG 500Å dC(bz), 30-40 mol/g CPG 500Å dG(ib), 30-40 mol/g CPG 500Å dT 30-40 mol/g CPG 500Å dA(bz), 30-40 mol/g CPG 500Å dC(bz), 30-40 mol/g CPG 500Å dG(ib), 30-40 mol/g CPG 500Å dT 30-40 mol/g CPG 1000Å dA(bz), 25-35 mol/g CPG 1000Å dC(bz), 25-35 mol/g CPG 1000Å dG(ib), 25-35 mol/g CPG 1000Å dT 25-35 mol/g CPG 1000Å dA(bz), 25-35 mol/g CPG 1000Å dC(bz), 25-35 mol/g CPG 1000Å dG(ib), 25-35 mol/g CPG 1000Å dT 25-35 mol/g

Unit
1x1g 1x1g 1x1g 1x1g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g 1x1g 1x1g 1x1g 1x1g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g C302001-01 G302001-01 A402001-01 C402001-01 G402001-01 C302010-01 C402010-01 G305001-01 G305010-01

Fast Deprotection RNA CPG
Catalog No.
A602001-01 C602001-01 G602001-01 U601001-01

Description
CPG 500Å rA(tac), 25-35 mol/g CPG 500Å rC(tac), 25-35 mol/g CPG 500Å rG(tac), 25-35 mol/g CPG 500Å rU, 25-35 mol/g

Unit
1x1g 1x1g 1x1g 1x1g

Customized packaging
CPG-products can be provided with higher loadings based on UHL-CPG (50–70 mol/g or 9 0 –110 mol/g), please inquire.

2’0-Methyl RNA CPG
Catalog No.
A601101-01 C602101-01 G601101-01 U601101-01

Description
CPG 500Å 2'O-Methyl-rA(bz), 30-40 mol/g CPG 500Å 2'O-Methyl-rC(tac), 30-40 mol/g CPG 500Å 2'O-Methyl-rG(ib), 30-40 mol/g CPG 500Å 2'O-Methyl-rU, 30-40 mol/g

Unit
1x1g 1x1g 1x1g 1x1g

42

Columns for Synthesizers

Columns for Synthesizers
In addition to our CPG free flow oligonucleotide synthesis supports, we offer ready to use synthesis columns filled with CPG or polystyrene

DNA Columns
Compatible with Expedite Instruments Catalog No.
A311010-01 C311010-01

Description
Column CPG 500Å dA(bz), 50 nmol Column CPG 500Å dC(bz), 50 nmol Column CPG 500Å dG(ib), 50 nmol Column CPG 500Å dT, 50 nmol Column CPG 500Å dA(bz), 0.2 mol Column CPG 500Å dC(bz), 0.2 mol Column CPG 500Å dG(ib), 0.2 mol Column CPG 500Å dT, 0.2 mol Column CPG 500Å dA(bz), 1 mol Column CPG 500Å dC(bz), 1 mol Column CPG 500Å dG(ib), 1 mol Column CPG 500Å dT, 1 mol Column CPG 1000Å dA(bz), 0.2 mol Column CPG 1000Å dC(bz), 0.2 mol Column CPG 1000Å dG(ib), 0.2 mol Column CPG 1000Å dT, 0.2 mol Column CPG 1000Å dA(bz), 1 mol Column CPG 1000Å dC(bz), 1 mol Column CPG 1000Å dG(ib), 1 mol Column CPG 1000Å dT, 1 mol

Unit
1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100

support. These columns are designed for use on
G311010-01

a wide range of synthesizers for high quality oligonucleotide synthesis. Our columns are produced at our high quality standard in order to be compliant in the required application and compatible with standard synthesis protocols.

T311010-01 A321010-01 C321010-01 G321010-01 T321010-01 A331010-01

Expedite columns
• Filled with CPG 500Å and CPG 1000Å • For Expedite 89er series only • Crimped • Color coded label

C331010-01 G331010-01 T331010-01 A421010-01 C421010-01 G421010-01 T421010-01

Cyclopeadic columns for ABI and Expedite synthesizer
• Filled with CPG 500Å and CPG 1000Å • Available in 50 nmol, 0.2 • No leakage (no crimping) • One column body • Color coded body, limits the wrong use of base in synthesis mol and 1 mol

A431010-01 C431010-01 G431010-01 T431010-01

Compatible with Expedite & ABI Instruments
A341010-01 C341010-01 G341010-01 T341010-01 A351010-01 Column CPG 500Å dA(bz), 50 nmol, ABI/89 Column CPG 500Å dC(bz), 50 nmol, ABI/89 Column CPG 500Å dG(ib), 50 nmol, ABI/89 Column CPG 500Å dT, 50 nmol, ABI/89 Column CPG 500Å dA(bz), 0.2 mol, ABI/89 Column CPG 500Å dC(bz), 0.2 mol, ABI/89 Column CPG 500Å dG(ib), 0.2 mol, ABI/89 Column CPG 500Å dT, 0.2 mol, ABI/89 Column CPG 500Å dA(bz), 1 mol, ABI/89 Column CPG 500Å dC(bz), 1 mol, ABI/89 Column CPG 500Å dG(ib), 1 mol, ABI/89 Column CPG 500Å dT, 1 mol, ABI/89 Column CPG 1000Å dA(bz), 0.2 mol, ABI/89 Column CPG 1000Å dC(bz), 0.2 mol, ABI/89 Column CPG 1000Å dG(ib), 0.2 mol, ABI/89 Column CPG 1000Å dT, 0.2 mol, ABI/89 Column CPG 1000Å dA(bz), 1 mol, ABI/89 Column CPG 1000Å dC(bz), 1 mol, ABI/89 Column CPG 1000Å dG(ib), 1 mol, ABI/89 Column CPG 1000Å dT 0.2 mol, ABI/89 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100

Columns for ABI 3900 synthesizers
• Filled with Polystyrene • For ABI 3900 synthesizers only • Available in 40 nmol, 0,2 mol

C351010-01 G351010-01 T351010-01 A361010-01 C361010-01 G361010-01

• Polystyrene 40 is comparable with CPG 1000Å • Color coded body, limits the wrong use of base in synthesis

T361010-01 A451010-01 C451010-01

RNA columns for ABI synthesizers
• Filled with CPG 500Å • For ABI 39X/3400 synthesizers only • Crimped • Color coded label

G451010-01 T451010-01 A461010-01 C461010-01 G461010-01 T461010-01

RNA columns for Expedite synthesizers
• Filled with CPG 500Å and CPG 1000Å • For ABI 39X/3400 synthesizers only • Crimped • Color coded label

43

Products

Columns for Synthesizers

DNA Columns (cont.)
Compatible with ABI 3900 Instruments Catalog No.
A911010-01 C911010-01 G915010-01 T911010-01 A921010-01 C921010-01 G925010-01 T921010-01

Description
Column Polystyrene 40 dA(bz), 40 nmol, ABI 3900 Column Polystyrene 40 dC(bz), 40 nmol, ABI 3900 Column Polystyrene 40 dG(dmf), 40 nmol, ABI 3900 Column Polystyrene 40 dT, 40 nmol, ABI 3900 Column Polystyrene 40 dA(bz), 0.2 µmol, ABI 3900 Column Polystyrene 40 dC(bz), 0.2 µmol, ABI 3900 Column Polystyrene 40 dG(dmf), 0.2 µmol, ABI 3900

Unit
1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100

Column Polystyrene 40 dT, 0.2 µmol, ABI 3900 1 x 100

Fast Deprotection RNA Columns
Compatible with Expedite Instruments Catalog No.
A632004-01 C632004-01 G632004-01 U631004-01

Description
Column CPG 500Å rA(tac), 1 mol Column CPG 500Å rC(tac), 1 mol Column CPG 500Å rG(tac), 1 mol Column CPG 500Å rU, 1 mol

Unit
1x4 1x4 1x4 1x4

Compatible with ABI Instruments
A662004-01 C662004-01 G662004-01 U661004-01 Column CPG 500Å rA(tac), 1 mol Column CPG 500Å rC(tac), 1 mol Column CPG 500Å rG(tac), 1 mol Column CPG 500Å rU, 1 mol 1x4 1x4 1x4 1x4

2’0-Methyl RNA Columns
Compatible with Expedite Instruments Catalog No.
A631104-01 C632104-01 G631104-01 U631104-01

Description
Column CPG 500Å 2'O-Me-rA(bz), 1 mol Column CPG 500Å 2'O-Me-rC(tac), 1 mol Column CPG 500Å 2'O-Me-rG(ib), 1 mol Column CPG 500Å 2'O-Me-rU, 1 mol

Unit
1x4 1x4 1x4 1x4

Compatible with ABI Instruments
A661104-01 C662104-01 G661104-01 U661104-01 Column CPG 500Å 2'O-Me-rA(bz), 1 mol Column CPG 500Å 2'O-Me-rC(tac), 1 mol Column CPG 500Å 2'O-Me-rG(ib), 1 mol Column CPG 500Å 2'O-Me-rU, 1 mol 1x4 1x4 1x4 1x4

44

Amino-ON CPG

Amino-ON CPG
Amino-ON CPG can be employed to conjugate biotin, fluorescein or other modifiers and reporter groups to the 3'-end of oligonucleotides

Amino-ON CPG
Bulk CPG Catalog No.
M020101-01 M020111-01

Description
Amino-ON CPG 500Å, 30-40 mol/g Amino-ON CPG 500Å, 30-40 mol/g

Unit
1 x 0.25 g 1x1g

or to attach oligonucleotides to surfaces. SAFC Supply Solutions’ Proligo Reagents Amino-ON CPG combines the advanced features of cleavage and deprotection under mild conditions, and maintenance of the integrity of the 3'-modification with high synthesis yields.

Key Features of 3'-Amine Oligonucleotides
• Selective conjugation to reporter groups, purification handles or other modifiers • 3'-end attachment to electrophilic surfaces • Offers protection against exonucleolytic degradation These features enable 3'-Amine oligonucleotides to be used in applications such as 3'-labeling with base sensitive dyes; 3'-biotin modifications; duallabeled probes (eg TaqMan probes and Molecular beacons); and microarray spotting.

Key Features of Amino-ON CPG
• Introduces a primary amino group attached to a C6-linker • Fully compatible with standard phosphoramidite reagents and synthesis conditions • Can be used with DNA, RNA and Locked Nucleic Acid® (LNA); 5'-oligonucleotide modifications such as fluorescein or biotin; and with the synthesis of thioated DNA oligonucleotides • Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotide can be cleaved from the support and deprotected in concentrated ammonia at 55°C in 2 hours • Provides all the advantages of homogeneous pore size porous glass supports • Standard pore size of 500Å • Loading of 30–40 mol/g

• Coupling performance of ≥99.0% stepwise efficiency • DMT-protected in the same way as standard nucleoside-loaded CPG

45

Products

Amino-ON CPG

Cleavage and deprotection
The cleavage and deprotection of oligonucleotides on amino-ON CPG can be performed with a variety of reagents. We have successfully applied the following reagents and conditions to simultaneously deprotect the hexylamino group and the oligonucleotide: • concentrated ammonia at 55°C for 2 hours or • 40% aqueous methylamine/concentrated ammonia 1/1, v/v, at 65°C for 10 minutes

Amino-ON CPG 500Å
Loading Storage: 30-40 ≤-10°C mol/g

Method
1. Attach the amino-ON CPG to the synthesizer in the same way as a nucleoside loaded CPG.

SAFC Supply Solutions’ Proligo Reagents Amino-ON CPG can be employed to introduce a covalently bound, primary amino group at the 3'-end of any oligonucleotide. Amino-ON CPG comprises a protected hexylamino group. The free amine is liberated under the alkaline conditions that are employed to cleave and deprotect the oligonucleotide on the support, while the hexylamino group remains attached to the oligonucleotide. The amino-ON CPG further comprises a DMT -protective group, which is removed in the first synthesis cycle similar to the DMT group of standard nucleoside loaded CPG. The synthesis of oligonucleotides is conducted in the same manner as with nucleoside-loaded CPG, without changes in any of the reagents of the synthesis. The only differences in the preparation of oligonucleotides using Proligo Reagents’ aminoON CPG are as follows: • the 3'-base of the oligonucleotide to be synthesized is not attached to the support: this base must be added in the first synthesis cycle • there is a simple modification to the cleavage and deprotection conditions, as detailed below Amino-ON CPG can be employed in the same manner to prepare 3'-amino DNA, RNA, LNA or 2’O-Methyl oligonucleotides. It can be applied with 5'-biotin, fluorescein and other oligonucleotide modifications, and for the synthesis of thioated DNA oligonucleotides. Proligo Reagents offers amino-ON CPG in bulk, with a pore size of 500Å.

2. Enter the sequence of the oligonucleotide you wish to synthesize. Note that the 3'-nucleoside of the oligonucleotide sequence must be included in the bases to be attached to the support during the synthesis. This can be achieved with a dummy nucleoside unit for the 3'-end of the sequence, e.g., add a thymidine unit to the 3'-end of the sequence. 3. Proceed as you would with a standard oligonucleotide synthesis. Depending on your intended further use of the oligomer you can either choose DMT -On or DMT -Off procedures. 4. The first deblocking step proceeds with the usual release of the orange colour of the dimethoxytrityl cation and may be utilized in trityl monitoring procedures. 5. Cleave and deprotect the oligonucleotide on the support using one of the conditions listed under “Cleavage and deprotection” above. 6. Transfer the supernatant solution of the oligonucleotide from the support into a separate vial. The yield of the oligonucleotide can be further improved by rinsing the support with water and combining the oligonucleotide solution with the washing solution. Evaporate to dryness. 7. The oligonucleotide is now ready for further processing, such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods.

46

Universal CPG

Universal CPG
Universal CPG from SAFC Supply Solutions can be employed in the synthesis of any oligonucleotide sequence instead of conventional nucleoside loaded CPG —

Universal CPG
Catalog No.
M301001-01 M301010-01 M401001-01 M401010-01

Description
Universal CPG 500Å, 30-40 Universal CPG 500Å, 30-40 Universal CPG 1000Å, 25-35 mol/g mol/g mol/g mol/g

Unit
1x1g 1 x 10 g 1x1g 1 x 10 g

whether loaded with adenosine, cytidine, guanosine or thymidine nucleosides. The use of universal CPG releases your resources as the purchase, handling and storage of differentially loaded types of nucleoside-CPG is not required, eliminating 3'-end synthesis errors, because of accidental interchange of polymeric supports.
CPG

Universal CPG 1000Å dT 25-35

O O
S p ac e r

N N O N

NH

N H

O

O

O OCH3

Key Features of Universal CPG
Fully compatible with standard phosphoramidite reagents and synthesis conditions Can be employed in the synthesis of many different types of oligonucleotides: • DNA oligonucleotides • Phosphorothioates • 2'O-Methyl oligonucleotides • Fluorescein, tetrachloro-fluorescein and biotin modifications • Particularly useful for high-throughput oligonucleotide synthesis • Coupling efficiency of ≥99.0% is routinely obtained • Convenient cleavage and deprotection • Avoids the use of unusual and potentially harmful reagents in the deprotection process e.g., heavy metals, sulfides

Universal CPG
Universal CPG Universal CPG Storage: 500Å Loading 30-40 1000Å Loading 25-35 ≤+10°C mol/g mol/g

47

Products

Universal CPG

Our universal CPG can be employed instead of conventional nucleoside-loaded CPG — whether loaded with adenosine, cytidine, guanosine or thymidine nucleosides, in the synthesis of any oligonucleotide sequence, irrespective of the 3'end nucleoside. The universal CPG contains an inosine-ribonucleotide adapter that is removed from the synthesized oligonucleotide during the cleavage and deprotection reaction. SAFC’s universal CPG can be used with the same standard conditions and reagents that are employed for the synthesis of oligonucleotides conducted with nucleoside-loaded CPG. The preparation differences of oligonucleotides using our universal CPG, are: • the 3'-base of the oligonucleotide to be synthesized is not attached to the support: this base must be added in the first synthesis cycle on the universal support • there is a simple modification to the cleavage and deprotection conditions, as detailed below • the first deblocking step — which is conducted in the usual manner — will not result in the release of the colored dimethoxytrityl cation from the support. This is because the inosineribonucleotide adapter contains a different acidlabile protective group (methoxyethylidene group) The universal CPG can be substituted for all conventional DNA supports, as well as supports loaded with 2'O-Methyl nucleosides and supports with other modified nucleosides. It can also be used with biotin and fluorescein phosphoramidites to produce 3'-modified oligonucleotides, and for the synthesis of thioated DNA oligonucleotides. Note: The use of universal CPG for the synthesis of RNA oligonucleotides or for any synthesis that involves base-labile nucleosides or other baselabile modifications, e.g., rhodamine dyes or Cy™3/Cy5, is not recommended.

Cleavage and deprotection
The cleavage of oligonucleotides from the universal CPG-support and the deprotection reaction can be performed with a variety of reagents. We have successfully applied the following reagents and conditions to completely cleave the inosine-ribonucleotide adapter from the oligonucleotide while simultaneously deprotecting the oligonucleotide: • concentrated ammonia/0.5 M lithium chloride solution 5/1, v/v, at 75°C for 6 hours • concentrated ammonia/0.5 M sodium chloride solution 5/1, v/v, at 75°C for 6 hours, or • 40% aqueous methylamine at 75°C for 6 hours

Method
1. Attach the universal CPG to the synthesizer in the same way as a nucleoside-loaded CPG. 2. Enter the sequence of the oligonucleotide you wish to synthesize. Note that the 3'-nucleoside of the oligonucleotide sequence must be included in the bases to be attached to the support during the synthesis. This can be achieved with a dummy nucleoside unit for the 3'-end of the sequence, e.g., add a thymidine unit to the 3'-end of the sequence. 3. Proceed as you would with a standard oligonucleotide synthesis. Depending on your intended further use of the oligomer, you can either choose DMT-On or DMT-Off procedures. 4. The first deblocking step proceeds without release of the orange color of the dimethoxytrityl cation. This step is nevertheless necessary to deprotect the inosine-ribonucleoside adapter of the universal CPG. 5. Cleave and deprotect the oligonucleotide on the support using one of the conditions listed under “Cleavage and deprotection” above. 6. Transfer the supernatant solution of the oligonucleotide from the support into a separate vial. The yield of the oligonucleotide can be further improved by rinsing the support with water and combining the oligonucleotide solution with the washing solution. Evaporate to dryness. 7. The oligonucleotide is now ready for further processing such as desalting or purification with RP-HPLC, AX-HPLC or gel-based methods.

48

Liquid Reagents

Liquid Reagents
In solid-phase synthesis of oligonucleotides, liquid reagents are used in each step of the synthesis cycle. Overall synthesis performance, and therefore total product yield and purity of the crude oligonucleotide, is highly dependent on the chemical purity of the monomers and the supporting liquid reagents. SAFC Supply Solutions has more than 25 years of manufacturing experience in DNA and RNA synthesis reagents. Our liquid reagents meet the most stringent quality specifications, making them ideal for use in automated nucleic acid synthesis. Lot-to-lot consistency is guaranteed for every product manufactured in our ISO 9001 certified site. Each lot is individually quality-controlled to ensure optimum synthesis performance.

Features
• Products designed for oligonucleotide synthesis • Liquid reagents offered in addition to amidites and supports • Constant high quality • Broad range of packaging • Customized formulations and specifications • Technical expertise

Benefits
• Constant high synthesis performance • Key oligonucleotide synthesis reagents available in one shop • Matching with high quality amidites and support products • Offering high flexibility • Special requirements will be fulfilled •Supporting customized solutions • Synthesis optimization and cost reduction

• New product development: Activator 42

49

Products

Liquid Reagents

=

B* = L = N = x = * =

protected base last cycle actual new cycle number of cycles B, is equivalent to BL in the cycle

Finalized Synthesis: Cleavage and Deprotection after x cycles:
BN HO BL O O P O O B O O P O

=

O

O H x-1

O O

BLac

Bac

O O P O OR

Start of Synthesis*: CPG Column loaded with first nucleoside:
B1ac x-1

=

O DMT O Start Cycle

Capping 2: Cap A and B solutions
BNac DMT O BLac Bac O x-1 O O P O OR

O

Detritylation: Deblock solution
BLac Bac

O O P O OR

=

=

HO

Oxidation: Oxidizer solution
BLac O Bac

O O P O OR

=

O x-1

O

O O P O OR

=

Capping 1: Cap A and B solution
x-1 BNac

BNac DMT O + O P

N(iPr)2 OR

O

Coupling
BLac Bac O O P O OR O O P O OR

Activator solution

=

O x-1

DMT O

The Oligonucleotide Synthesis Cycle For TAC-protected G-amidites (dG(tac) phoshoramidite, rG(tac) phosphoramidite) Fast Deprotection Cap A must be employed to prevent transacylation side reactions on the guanine base.

Chemical Composition and Purpose of Proligo Reagents’ Liquid Reagents during the Synthesis Cycle
Deblock solution, containing trichloracetic acid (TCA) or dichloroacetic acid (DCA) in dichloromethane, removes the dimethoxytrityl (DMT) protecting group from the 5'hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5'hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction. Wash solution, containing anhydrous acetonitrile (water content ≤30 ppm), is used as the overall wash solvent following each step in the synthesis cycle. Activator solution, containing powerful activators like 4,5-dicyanoimidazole (DCI) or 5-(3,5bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5'hydroxyl group of the growing oligonucleotide chain. Cap A solution, containing acetic anhydride and tetrahydrofuran (THF), is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5'hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5'hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps. For synthesis with Proligo Reagents’ TAC-protected phosphoramidites, Fast Deprotection CAP A solution must be employed instead of Cap A solution. Fast Deprotection Cap A solution must be used for synthesis with Proligo Reagents’ TAC-protected phosphoramidites. Fast Deprotection Cap A solution, containing tert-butylphenoxyacetyl acetic anhydride (tac2O) in tetrahydrofuran, is used in place of Cap A solution to ensure that the displacement of tert-butylphenoxyacetyl (TAC) on guanine bases of the TAC-protected RNA phosphoramidites does not occur. Cap B solution, containing tetrahydrofuran (THF), pyridine and N-methylimidazole (NMI), is mixed in situ with Cap A solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst. Oxidizer solution, containing tetrahydrofuran (THF), pyridine, iodine and water, is applied to oxidize the chemically unstable trivalent phosphorous triester linkage formed in the coupling reaction to a stable pentavalent phosphate triester. Iodine functions as a mild oxidant and water functions as an oxygen donor.

50

=

Custom products
Inquire for customized liquid reagents with specialty formulations or alternative packaging.

Bulk
Packaging in drums or m3-containers is available upon request.

Liquid Reagents
Catalog No.
L010010-06 L010250-04 L010400-04 L011800-01

Liquid Reagents (continued)
Unit
6 x 100 ml 4 x 2.5 L 4x4L 1 x 18 L 1 x 45 L 1 x 200 L 1 x 1400 L 4 x 2.5 L 4 x 2.5 L 4 x 2.5 L 4x4L 1 x 25 L 4 x 2.5 L 4 x 2.5 L 4 x 2.5 L 1 x 2.5 L 4 x 2.5 L 1 x 2.5 L 1x4L 1 x 2.5 L 4 x 2.5 L 1 x 2.5 L 1x4L 4 x 2.5 L 4 x 2.5 L 4 x 2.5 L 4x4L 1 x 2.5 L 1x4L 4 x 2.5 L 4 x 2.5 L 4x4L 1 x 25 L

Description
Amidite Diluent (Acetonitrile) Acetonitrile Acetonitrile Acetonitrile

Compatible with ABI Instruments
L260045-06 L320045-01 L3300182-06 L8300452-06 L8300462-06 L8300451-06 L340018-06 L340045-06 L350018-06 L350045-06 L360020-06 L360045-06 L860021-06 L370018-06 L370045-06 L380018-06 L880045-06 Oxidizer 0.02 M for ABI Instruments TCA Deblock for ABI Instruments Activator 42 0.1 M Activator 42 0.1 M Activator 42 0.25 M ETT Activator 0.25 M Cap A for ABI Instruments Cap A for ABI Instruments Cap B for ABI Instruments Cap B for ABI Instruments Oxidizer 0.1 M for ABI Instruments Oxidizer 0.1 M for ABI Instruments Oxidizer 0.02 M for ABI Instruments Fast Deprotection Cap A for ABI Instruments Fast Deprotection Cap A DCI Activator 0.25 M for ABI Instruments DCI Activator 0.25 M 6 x 450 ml 1 x 450 ml 6 x 180 ml 6 x 450 ml 6 x 450 ml 6 x 450 ml 6 x 180 ml 6 x 450 ml 6 x 180 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 180 ml 6 x 450 ml 6 x 180 ml 6 x 450 ml

L014500-C01-01 Acetonitrile L0120000-C01-01 Acetonitrile L0114000-01 L020250-04 L0202502-04 L020251-04 L020400-04 L022500-01 L0302502-04 L0302512-04 L0302501-04 L040250-01 L040250-04 L040251-01 L040400-01 L050250-01 L050250-04 L050251-01 L050400-01 L060250-04 L060251-04 L060252-04 L060400-04 L070250-01 L070400-01 L080250-04 L080251-04 L080400-04 L082500-01 Acetonitrile TCA Deblock TFA Deblock Toluene DCA Deblock TCA Deblock TCA Deblock Activator 42 0.1 M Activator 42 0.25 M ETT Activator 0.25 M Cap A Cap A Cap A for ABI Instruments Cap A Cap B Cap B Cap B for ABI Instruments Cap B Oxidizer 0.02 M Oxidizer 0.1 M for ABI Instruments Oxidizer 0.02 M for ABI 3900 Instruments Oxidizer 0.02 M Fast Deprotection Cap A Fast Deprotection Cap A DCI Activator 0.25 M DCI Activator 0.5 M DCI Activator 0.25 M DCI Activator 0.25 M

Compatible with Expedite and Polygen Instruments L810045-06 L820090-06 L8300202-06 L8300212-06 L830045-06 L8300462-06 L840020-06 L840045-06 L850020-06 L850045-06 L860020-06 L860045-06 L870020-06 L370045-06 L880020-06 L880045-06 Acetonitrile for 8900 Instruments TCA Deblock for 8900 Instruments Activator 42 0.1 M Activator 42 0.25 M Activator 42 0.1 M Activator 42 0.25 M Cap A for 8900 Instruments Cap A for 8900 Instruments Cap B for 8900 Instruments Cap B for 8900 Instruments Oxidizer 0.02 M for 8900 Instruments Oxidizer 0.02 M for 8900 Instruments Fast Deprotection Cap A for 8900 Instruments Fast Deprotection Cap A DCI Activator 0.25 M for 8900 Instruments DCI Activator 0.25 M 6 x 450 ml 6 x 900 ml 6 x 200 ml 6 x 200 ml 6 x 450 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml

ACN Based Liquid Reagents
LB40250-01 LB50250-01 LR40250-01 LR50250-01 LR60250-04 Cap A ACN Cap B ACN Cap 1 in ACN Fast Deprotection Cap 2 in ACN Oxidizer in ACN 1 x 2.5 L 1 x 2.5 L 1 x 2.5 L 1 x 2.5 L 4 x 2.5 L

Compatible with Äkta and Oligo Pilot Instruments
L520251-04 L540250-01 L550250-01 L550251-01 L560250-04 DCA Deblock (Toluene) Cap A for Äkta and Oligo Pilot Cap B1 for Äkta and Oligo Pilot Cap B2 for Äkta and Oligo Pilot Oxidizer 0.05 M for Äkta and Oligo Pilot 4 x 2.5 L 1 x 2.5 L 1 x 1.25 L 1 x 1.25 L 4 x 2.5 L

51

Products

Activator 42

Activator 42®
Activator 42 introduces unprecedented activation power to phosphoramidite coupling reactions. This novel activator provides unique opportunities to speed up oligonucleotide synthesis and to improve on product yield. Whereas deblocking and oxidation reactions proceed with nearly quantitative yields in state-ofthe-art oligonucleotide synthesis, the coupling reactions, although high yielding, determine the overall efficiency of the process. Coupling yields are driven by a variety of factors, including amidite and solid support quality, water content of the coupling medium and the choice of activator molecule employed. An ideal activator should promote the coupling of standard DNA amidites as well as the coupling of more sterically demanding amidites (e.g. 2'O-TBDMS-RNA amidites) in a highly efficient manner. The activator should have excellent solubility in the coupling medium (i.e., acetonitrile) in order to avoid undesired precipitation or crystallization, should not be hygroscopic, should be safe to handle and should not lead to side reactions in oligonucleotide synthesis. These desired properties can be attributed to a new powerful activator molecule which gives superior performance compared to the other commercial activators such as 5-ethylthio-1H-tetrazole (ETT), 5-benzylthio-1H-tetrazole (BTT) and 4,5-dicyanoimidazole (DCI). Activation efficiency for 2'O-TBDMS-RNA amidites Activator 42 > ETT = BTT Activation efficiency for standard DNA amidites Activator 42 > DCI Solid phase oligonucleotide synthesis with phosphoramidites consists of a sequence of three consecutive reaction steps which are repeated in a cyclical manner, deblocking, coupling and oxidation.

Key features of Activator 42
• The highest activation efficiency in the industry compared to other activators • Promotes ultrafast DNA couplings (coupling time less than 15 seconds) • Promotes rapid RNA couplings (coupling time 6 minutes) • Excellent solubility in acetonitrile (greater than 0.9 M) • Not hygroscopic • Safe to handle (no explosive properties)

Activator 42
Catalog No.
L0302502-04 L3300182-06 L8300452-06 L8300202-06 L0302512-04

Description
Activator 42 0.1 M Activator 42 0.1 M Activator 42 0.1 M Activator 42 0.1 M Activator 42 0.25 M Activator 42 0.25 M Activator 42 0.25 M

Unit
4 x 2.5 L 6 x 180 ml 6 x 450 ml 6 x 200 ml 4 x 2.5 L 6 x 450 ml 6 x 200 ml

• Very high overall yield in DNA and RNA synthesis

L8300462-06 L8300212-06

Other quantities and packaging are available upon request.

52

Product List
DNA Pharmadites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 RNA Pharmadites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Standard DNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Fast Deprotection Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 RNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Fast Deprotection RNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 2’0-Methyl RNA Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 DMT-2'Fluoro Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 Labels and Modifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 Non-Standard Nucleosides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 Phosphate-ON Phosphoramidite . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 NPPOC Phosphoramidites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 DNA CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 Fast Deprotection CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 Fast Deprotection RNA CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 2’0-Methyl RNA CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 DNA Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Fast Deprotection RNA Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 2’0-Methyl RNA Columns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 Amino-ON CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 Universal CPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 Liquid Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 Activator 42 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

53

Products

Product List

DNA Pharmadites
Catalog No.
A111P00-C C111P00-C G111P00-C T111P00-C

Standard DNA Phosphoramidites (cont.)
Compatible with MerMade Instruments (8oz 28/400 bottle)
A111085-06 C111085-06 G111085-06 T111085-06 A111028-06 C111028-06 G111028-06 T111028-06 DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite 6x5g 6x5g 6x5g 6x5g 6 x 10 g 6 x 10 g 6 x 10 g 6 x 10 g

Description
DMT-dA(bz) Pharmadite DMT-dC(bz) Pharmadite DMT-dG(ib) Pharmadite DMT-dT Pharmadite

RNA Pharmadites
A211P00-C C213P00-C G211P00-C U211P00-C DMT-2'O-TBDMS-rA(bz) Pharmadite DMT-2'O-TBDMS-rC(ac) Pharmadite DMT-2'O-TBDMS-rG(ib) Pharmadite DMT-2'O-TBDMS-rU Pharmadite

Compatible with Äkta and Oligo Pilot Instruments (100 ml septum bottle)
A111005-01 C111005-01 DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite 1x5g 1x5g 1x5g 1x5g 6x5g 6x5g 6x5g 6x5g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g

Standard DNA Phosphoramidites
Compatible with Expedite and Polygen Instruments Catalog No.
A111081-12 C111081-12 G111081-12 T111081-12 A111082-12 C111082-12 G111082-12 T111082-12

G111005-01 T111005-01

Description
DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite

Unit
12 x 1 g 12 x 1 g 12 x 1 g 12 x 1 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 2 g

A111005-06 C111005-06 G111005-06 T111005-06 A111010-01 C111010-01 G111010-01 T111010-01

Bulk Quantities (16 oz 28/400 bottle)
A111021-06 DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite 6 x 10 g 6 x 10 g 6 x 10 g 6 x 10 g 6 x 20 g 6 x 20 g 6 x 20 g 6 x 20 g

Compatible with ABI Instruments
A111031-12 C111031-12 G111031-12 T111031-12 A111032-12 C111032-12 G111032-12 T111032-12 A111064-12 C111064-12 G111064-12 T111064-12 DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite DMT-dA(bz) Amidite DMT-dC(bz) Amidite DMT-dG(ib) Amidite DMT-dT Amidite 12 x 1 g 12 x 1 g 12 x 1 g 12 x 1 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 4 g 12 x 4 g 12 x 4 g 12 x 4 g

C111021-06 G111021-06 T111021-06 A111020-06 C111020-06 G111020-06 T111020-06

54

Fast Deprotection Phosphoramidites
Compatible with Expedite and Polygen Instruments Catalog No.
C113081-12 C113082-12 A112081-12 C112081-12 G112081-12 A112082-12 C112082-12 G112082-12 G115081-12 G115082-12

RNA Phosphoramidites
Compatible with Expedite and Polygen Instruments

Description
DMT-dC(ac) Amidite DMT-dC(ac) Amidite DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dG(dmf) Amidite DMT-dG(dmf) Amidite

Unit
12 x 1 g 12 x 2 g 12 x 1 g 12 x 1 g 12 x 1 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 1 g 12 x 2 g

Catalog No.
A211081-01 C213081-01 G211081-01 U211081-01

Description
DMT-2'O-TBDMS-rA(bz) Amidite DMT-2'O-TBDMS-rC(ac) Amidite DMT-2'O-TBDMS-rG(ib) Amidite DMT-2'O-TBDMS-rU Amidite

Unit
1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g

Compatible with ABI Instruments
A211031-01 C213031-01 G211031-01 U211031-01 A211061-01 C213061-01 DMT-2'O-TBDMS-rA(bz) Amidite DMT-2'O-TBDMS-rC(ac) Amidite DMT-2'O-TBDMS-rG(ib) Amidite DMT-2'O-TBDMS-rU Amidite DMT-2'O-TBDMS-rA(bz) Amidite DMT-2'O-TBDMS-rC(ac) Amidite DMT-2'O-TBDMS-rG(ib) Amidite DMT-2'O-TBDMS-rU Amidite 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1x1g 1x1g 1x1g 1x1g

Compatible with ABI Instruments
A112031-12 C113031-12 C113032-12 C112031-12 G112031-12 A112032-12 C112032-12 G112032-12 A112064-12 C112064-12 G115031-12 G115032-12 G115064-12 DMT-dA(tac) Amidite DMT-dC(ac) Amidite DMT-dC(ac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(dmf) Amidite DMT-dG(dmf) Amidite DMT-dG(dmf) Amidite 12 x 4 g 12 x 1 g 12 x 2 g 12 x 1 g 12 x 1 g 12 x 2 g 12 x 2 g 12 x 2 g 12 x 4 g 12 x 4 g 12 x 1 g 12 x 2 g 12 x 4 g

G211061-01 U211061-01

Fast Deprotection RNA Phosphoramidites
Compatible with Expedite and Polygen Instruments Catalog No.
A212081-01 C212081-01 G212081-01 U211081-01

Description
DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite

Unit
1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g

Compatible with ABI Instruments
A212031-01 C212031-01 DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1x1g 1x1g 1x1g 1x1g

Compatible with MerMade Instruments (8oz 28/400 bottle)
C113085-06 C112028-01 C112085-06 G115028-06 G115085-06 DMT-dC(ac) Amidite DMT-dC(tac) Amidite DMT-dC(tac) Amidite DMT-dG(dmf) Amidite DMT-dG(dmf) Amidite 6x5g 1 x 10 g 6x5g 6 x 10 g 6x5g

G212031-01 U211031-01 A212061-01 C212061-01 G212061-01 U211061-01

Compatible with Äkta and Oligo Pilot Instruments (100 ml septum bottle)
A112010-01 C112010-01 G112010-01 G115005-01 DMT-dA(tac) Amidite DMT-dC(tac) Amidite DMT-dG(tac) Amidite DMT-dG(dmf) Amidite 1 x 10 g 1 x 10 g 1 x 10 g 1x5g

Compatible with MerMade Instruments
A212028-06 C212028-06 G212028-06 U211028-06 DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite 6 x 10 g 6 x 10 g 6 x 10 g 6 x 10 g

Bulk Quantities (16 oz 28/400 bottle)
C113020-06 C112021-06 C112020-06 G115021-06 DMT-dC(ac) Amidite DMT-dC(tac) Amidite DMT-dC(tac) Amidite DMT-dG(dmf) Amidite 6 x 20 g 6 x 10 g 6 x 20 g 6 x 10 g

Bulk Quantities
A212010-01 C212010-01 G212010-01 U211010-01 DMT-2'O-TBDMS-rA(tac) Amidite DMT-2'O-TBDMS-rC (tac) Amidite DMT-2'O-TBDMS-rG(tac) Amidite DMT-2'O-TBDMS-rU Amidite 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g

55

Products

Product List

2’0-Methyl RNA Phosphoramidites
Catalog No. Description Unit Compatible with Expedite Instruments
A211181-01 A212181-01 C212181-01 C213181-01 G211181-01 G212181-01 U211181-01 DMT-2'O-Me-rA(bz) Amidite DMT-2'O-Me-rA(tac) Amidite DMT-2'O-Me-rC(tac) Amidite DMT-2'O-Me-rC(ac) Amidite DMT-2'O-Me-rG(ib) Amidite DMT-2'O-Me-rG(tac) Amidite DMT-2'O-Me-rU Amidite 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g

Labels and Modifications
Compatible with Expedite and Polygen Instruments Catalog No.
M010982-01 M010882-01 M010181-01 M010282-01 M010381-01 M010382-01 M010682-01

Description
ssH-Linker TFA Hexylaminolinker Fluorescein Amidite MMT-Hexylamine-Linker Amidite Biotin Amidite Biotin Amidite TFA Pentylaminolinker Amidite

Unit
1 x 0.25 g 1 x 0.25 g 1 x 0.1 g 1 x 0.25 g 1 x 0.1 g 1 x 0.25 g 1 x 0.25 g

Compatible with ABI Instruments
A211131-01 A212131-01 C212131-01 C213131-01 G211131-01 G212131-01 U211131-01 A211161-01 C212161-01 C213161-01 G211161-01 U211161-01 DMT-2'O-Me-rA(bz) Amidite DMT-2'O-Me-rA(tac) Amidite DMT-2'O-Me-rC(tac) Amidite DMT-2'O-Me-rC(ac) Amidite DMT-2'O-Me-rG(ib) Amidite DMT-2'O-Me-rG(tac) Amidite DMT-2'O-Me-rU Amidite DMT-2'O-Me-rA(bz) Amidite DMT-2'O-Me-rC(tac) Amidite DMT-2'O-Me-rC(ac) Amidite DMT-2'O-Me-rG(ib) Amidite DMT-2'O-Me-rU Amidite 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1 x 0.5 g 1x1g 1x1g 1x1g 1x1g 1x1g

Compatible with ABI Instruments
M010932-01 M010832-01 M010131-01 M010232-01 M010331-01 M010332-01 M010632-01 ssH-Linker TFA Hexylaminolinker Amidite Fluorescein Amidite MMT-Hexylamine-Linker Amidite Biotin Amidite Biotin Amidite TFA Pentylaminolinker Amidite 1 x 0.25 g 1 x 0.25 g 1 x 0.1 g 1 x 0.25 g 1 x 0.1 g 1 x 0.25 g 1 x 0.25 g

Non-Standard Nucleosides
Compatible with Expedite and Polygen Instruments Catalog No.
M113381-01

Description
5-Methyl-dC(ac) Amidite DMT-dInosine Amidite DMT-dUridine Amidite

Unit
1 x 0.25 g 1 x 0.25 g 1 x 0.25 g

Bulk Quantities
A211110-01 A212110-01 C212110-01 G211110-01 G212110-01 U211110-01 DMT-2'O-Me-rA(bz) Amidite DMT-2'O-Me-rA(tac) Amidite DMT-2'O-Me-rC(tac) Amidite DMT-2'O-Me-rG(ib) Amidite DMT-2'O-Me-rG(tac) Amidite DMT-2'O-Me-rU Amidite 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g

M111181-01 M110281-01

Compatible with ABI Instruments
M113331-01 M111131-01 M110231-01 5-Methyl-dC(ac) Amidite DMT-dInosine Amidite DMT-dUridine Amidite 1 x 0.25 g 1 x 0.25 g 1 x 0.25 g

DMT -2'Fluoro Phosphoramidites
Catalog No.
C213281-01 C213231-01 C213233-01 U211281-01 U211231-01 U211233-01

Phosphate-ON Phosphoramidite
Unit
1 x 0.25 g 1 x 0.25 g 1x1g 1 x 0.25 g 1 x 0.25 g 1x1g

Description
DMT-2'Fluoro-dC(ac) Amidite, 89 DMT-2'Fluoro-dC(ac) Amidite, ABI DMT-2'Fluoro-dC(ac) Amidite, ABI DMT-2'Fluoro-dU Amidite, 89 DMT-2'Fluoro-dU Amidite, ABI DMT-2'Fluoro-dU Amidite, ABI

Compatible with Expedite and Polygen Instruments Catalog No.
M010782-01

Description
Phosphate-ON Amidite

Unit
1 x 0.25 g

Compatible with ABI Instruments
M010732-01 Phosphate-ON Amidite 1 x 0.25 g

NPPOC Phosphoramidites
Catalog No.
A112N01-01 C114N01-01 G114N01-01 T111N01-01

Description
NPPOC-dA(tac) Amidite NPPOC-dC(ib) Amidite NPPOC-dG(ipac) Amidite NPPOC-dT Amidite

Unit
1x1g 1x1g 1x1g 1x1g

56

DNA CPG
Catalog No.
A301001-01 C301001-01 G301001-01 T301001-01 A301010-01 C301010-01 G301010-01 T301010-01 A401001-01 C401001-01 G401001-01 T401001-01 A401010-01 C401010-01 G401010-01 T401010-01

Fast Deprotection RNA CPG
Description
CPG 500Å dA(bz), 30-40 mol/g CPG 500Å dC(bz), 30-40 mol/g CPG 500Å dG(ib), 30-40 mol/g CPG 500Å dT 30-40 mol/g CPG 500Å dA(bz), 30-40 mol/g CPG 500Å dC(bz), 30-40 mol/g CPG 500Å dG(ib), 30-40 mol/g CPG 500Å dT 30-40 mol/g CPG 1000Å dA(bz), 25-35 mol/g CPG 1000Å dC(bz), 25-35 mol/g CPG 1000Å dG(ib), 25-35 mol/g CPG 1000Å dT 25-35 mol/g CPG 1000Å dA(bz), 25-35 mol/g CPG 1000Å dC(bz), 25-35 mol/g CPG 1000Å dG(ib), 25-35 mol/g CPG 1000Å dT 25-35 mol/g

Unit
1x1g 1x1g 1x1g 1x1g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g 1x1g 1x1g 1x1g 1x1g 1 x 10 g 1 x 10 g 1 x 10 g 1 x 10 g

Catalog No.
A602001-01 C602001-01 G602001-01 U601001-01

Description
CPG 500Å rA(tac), 25-35 mol/g CPG 500Å rC(tac), 25-35 mol/g CPG 500Å rG(tac), 25-35 mol/g CPG 500Å rU, 25-35 mol/g

Unit
1x1g 1x1g 1x1g 1x1g

2’0-Methyl RNA CPG
Catalog No.
A601101-01 C602101-01 G601101-01 U601101-01

Description
CPG 500Å 2'O-Methyl-rA(bz), 30-40 mol/g CPG 500Å 2'O-Methyl-rC(tac), 30-40 mol/g CPG 500Å 2'O-Methyl-rG(ib), 30-40 mol/g CPG 500Å 2'O-Methyl-rU, 30-40 mol/g

Unit
1x1g 1x1g 1x1g 1x1g

Fast Deprotection CPG
Catalog No.
C303001-01 C303010-01 C403001-01 C403010-01 A302001-01 C302001-01 G302001-01 A402001-01 C402001-01 G402001-01 C302010-01 C402010-01 G305001-01 G305010-01

Description
CPG 500Å dC(ac) 30-40 mol/g CPG 500Å dC(ac) 30-40 mol/g CPG 1000Å dC(ac) 25-35 mol/g CPG 1000Å dC(ac) 25-35 mol/g CPG 500Å dA(tac), 30-40 mol/g CPG 500Å dC(tac), 30-40 mol/g CPG 500Å dG(tac), 30-40 mol/g CPG 1000Å dA(tac), 25-35 mol/g CPG 1000Å dC(tac), 25-35 mol/g CPG 1000Å dG(tac), 25-35 mol/g CPG 500Å dC(tac), 30-40 mol/g CPG 1000Å dC(tac), 25-35 mol/g CPG 500Å dG(dmf), 30-40 mol/g CPG 500Å dG(dmf), 30-40 mol/g

Unit
1x1g 1 x 10 g 1x1g 1 x 10 g 1x1g 1x1g 1x1g 1x1g 1x1g 1x1g 1 x 10 g 1 x 10 g 1x1g 1 x 10 g

57

Products

Product List

DNA Columns
Compatible with Expedite Instruments Catalog No.
A311010-01 C311010-01 G311010-01 T311010-01 A321010-01 C321010-01 G321010-01 T321010-01 A331010-01 C331010-01 G331010-01 T331010-01 A421010-01 C421010-01 G421010-01 T421010-01 A431010-01 C431010-01 G431010-01 T431010-01

DNA Columns (cont.)
Compatible with ABI 3900 Instruments Unit
1 x 100 1 x 100 C911010-01 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 T921010-01 C921010-01 G925010-01 G915010-01 T911010-01 A921010-01

Description
Column CPG 500Å dA(bz), 50 nmol Column CPG 500Å dC(bz), 50 nmol Column CPG 500Å dG(ib), 50 nmol Column CPG 500Å dT, 50 nmol Column CPG 500Å dA(bz), 0.2 mol Column CPG 500Å dC(bz), 0.2 mol Column CPG 500Å dG(ib), 0.2 mol Column CPG 500Å dT, 0.2 mol Column CPG 500Å dA(bz), 1 mol Column CPG 500Å dC(bz), 1 mol Column CPG 500Å dG(ib), 1 mol Column CPG 500Å dT, 1 mol Column CPG 1000Å dA(bz), 0.2 mol Column CPG 1000Å dC(bz), 0.2 mol Column CPG 1000Å dG(ib), 0.2 mol Column CPG 1000Å dT, 0.2 mol Column CPG 1000Å dA(bz), 1 mol Column CPG 1000Å dC(bz), 1 mol Column CPG 1000Å dG(ib), 1 mol Column CPG 1000Å dT, 1 mol

Catalog No.
A911010-01

Description
Column Polystyrene 40 dA(bz), 40 nmol, ABI 3900 Column Polystyrene 40 dC(bz), 40 nmol, ABI 3900 Column Polystyrene 40 dG(dmf), 40 nmol, ABI 3900 Column Polystyrene 40 dT, 40 nmol, ABI 3900 Column Polystyrene 40 dA(bz), 0.2 µmol, ABI 3900 Column Polystyrene 40 dC(bz), 0.2 µmol, ABI 3900 Column Polystyrene 40 dG(dmf), 0.2 µmol, ABI 3900

Unit
1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100

Column Polystyrene 40 dT, 0.2 µmol, ABI 3900 1 x 100

Fast Deprotection RNA Columns
Compatible with Expedite Instruments Catalog No.
A632004-01 C632004-01 G632004-01 U631004-01

Description
Column CPG 500Å rA(tac), 1 mol Column CPG 500Å rC(tac), 1 mol Column CPG 500Å rG(tac), 1 mol Column CPG 500Å rU, 1 mol

Unit
1x4 1x4 1x4 1x4

Compatible with Expedite & ABI Instruments
A341010-01 C341010-01 G341010-01 T341010-01 A351010-01 C351010-01 G351010-01 T351010-01 A361010-01 C361010-01 G361010-01 T361010-01 A451010-01 C451010-01 G451010-01 T451010-01 A461010-01 C461010-01 G461010-01 T461010-01 Column CPG 500Å dA(bz), 50 nmol, ABI/89 Column CPG 500Å dC(bz), 50 nmol, ABI/89 Column CPG 500Å dG(ib), 50 nmol, ABI/89 Column CPG 500Å dT, 50 nmol, ABI/89 Column CPG 500Å dA(bz), 0.2 mol, ABI/89 Column CPG 500Å dC(bz), 0.2 mol, ABI/89 Column CPG 500Å dG(ib), 0.2 mol, ABI/89 Column CPG 500Å dT, 0.2 mol, ABI/89 Column CPG 500Å dA(bz), 1 mol, ABI/89 Column CPG 500Å dC(bz), 1 mol, ABI/89 Column CPG 500Å dG(ib), 1 mol, ABI/89 Column CPG 500Å dT, 1 mol, ABI/89 Column CPG 1000Å dA(bz), 0.2 mol, ABI/89 Column CPG 1000Å dC(bz), 0.2 mol, ABI/89 Column CPG 1000Å dG(ib), 0.2 mol, ABI/89 Column CPG 1000Å dT, 0.2 mol, ABI/89 Column CPG 1000Å dA(bz), 1 mol, ABI/89 Column CPG 1000Å dC(bz), 1 mol, ABI/89 Column CPG 1000Å dG(ib), 1 mol, ABI/89 Column CPG 1000Å dT 0.2 mol, ABI/89 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100 1 x 100

Compatible with ABI Instruments
A662004-01 C662004-01 G662004-01 U661004-01 Column CPG 500Å rA(tac), 1 mol Column CPG 500Å rC(tac), 1 mol Column CPG 500Å rG(tac), 1 mol Column CPG 500Å rU, 1 mol 1x4 1x4 1x4 1x4

2’0-Methyl RNA Columns
Compatible with Expedite Instruments Catalog No.
A631104-01 C632104-01 G631104-01 U631104-01

Description
Column CPG 500Å 2'O-Me-rA(bz), 1 mol Column CPG 500Å 2'O-Me-rC(tac), 1 mol Column CPG 500Å 2'O-Me-rG(ib), 1 mol Column CPG 500Å 2'O-Me-rU, 1 mol

Unit
1x4 1x4 1x4 1x4

Compatible with ABI Instruments
A661104-01 C662104-01 G661104-01 U661104-01 Column CPG 500Å 2'O-Me-rA(bz), 1 mol Column CPG 500Å 2'O-Me-rC(tac), 1 mol Column CPG 500Å 2'O-Me-rG(ib), 1 mol Column CPG 500Å 2'O-Me-rU, 1 mol 1x4 1x4 1x4 1x4

58

Amino-ON CPG
Bulk CPG Catalog No.
M020101-01 M020111-01

Liquid Reagents
Catalog No. Description
Amidite Diluent (Acetonitrile) Acetonitrile Acetonitrile Acetonitrile

Unit
6 x 100 ml 4 x 2.5 L 4x4L 1 x 18 L 1 x 45 L 1 x 200 L 1 x 1400 L 4 x 2.5 L 4 x 2.5 L 4 x 2.5 L 4x4L 1 x 25 L 4 x 2.5 L 4 x 2.5 L 4 x 2.5 L 1 x 2.5 L 4 x 2.5 L 1 x 2.5 L 1x4L 1 x 2.5 L 4 x 2.5 L 1 x 2.5 L 1x4L 4 x 2.5 L 4 x 2.5 L 4 x 2.5 L 4x4L 1 x 2.5 L 1x4L 4 x 2.5 L 4 x 2.5 L 4x4L 1 x 25 L

Description
Amino-ON CPG 500Å, 30-40 mol/g Amino-ON CPG 500Å, 30-40 mol/g

Unit
1 x 0.25 g 1x1g

L010010-06 L010250-04 L010400-04 L011800-01

L014500-C01-01 Acetonitrile

Universal CPG
Catalog No.
M301001-01 M301010-01 M401001-01 M401010-01

L0120000-C01-01 Acetonitrile

Description
Universal CPG 500Å, 30-40 Universal CPG 500Å, 30-40 Universal CPG 1000Å, 25-35 mol/g mol/g mol/g mol/g

Unit
1x1g 1 x 10 g 1x1g 1 x 10 g

L0114000-01 L020250-04 L0202502-04 L020251-04 L020400-04 L022500-01 L0302502-04 L0302512-04 L0302501-04 L040250-01 L040250-04 L040251-01 L040400-01 L050250-01 L050250-04 L050251-01 L050400-01 L060250-04 L060251-04 L060252-04 L060400-04 L070250-01 L070400-01 L080250-04 L080251-04 L080400-04 L082500-01

Acetonitrile TCA Deblock TFA Deblock Toluene DCA Deblock TCA Deblock TCA Deblock Activator 42 0.1 M Activator 42 0.25 M ETT Activator 0.25 M Cap A Cap A Cap A for ABI Instruments Cap A Cap B Cap B Cap B for ABI Instruments Cap B Oxidizer 0.02 M Oxidizer 0.1 M for ABI Instruments Oxidizer 0.02 M for ABI 3900 Instruments Oxidizer 0.02 M Fast Deprotection Cap A Fast Deprotection Cap A DCI Activator 0.25 M DCI Activator 0.5 M DCI Activator 0.25 M DCI Activator 0.25 M

Universal CPG 1000Å dT 25-35

ACN Based Liquid Reagents
LB40250-01 LB50250-01 LR40250-01 LR50250-01 LR60250-04 Cap A ACN Cap B ACN Cap 1 in ACN Fast Deprotection Cap 2 in ACN Oxidizer in ACN 1 x 2.5 L 1 x 2.5 L 1 x 2.5 L 1 x 2.5 L 4 x 2.5 L

59

Products

Product List

Liquid Reagents (continued)
Compatible with ABI Instruments
L260045-06 L320045-01 L3300182-06 L8300452-06 L8300462-06 L8300451-06 L340018-06 L340045-06 L350018-06 L350045-06 L360020-06 L360045-06 L860021-06 L370018-06 L370045-06 L380018-06 L880045-06 Oxidizer 0.02 M for ABI Instruments TCA Deblock for ABI Instruments Activator 42 0.1 M Activator 42 0.1 M Activator 42 0.25 M ETT Activator 0.25 M Cap A for ABI Instruments Cap A for ABI Instruments Cap B for ABI Instruments Cap B for ABI Instruments Oxidizer 0.1 M for ABI Instruments Oxidizer 0.1 M for ABI Instruments Oxidizer 0.02 M for ABI Instruments Fast Deprotection Cap A for ABI Instruments Fast Deprotection Cap A DCI Activator 0.25 M for ABI Instruments DCI Activator 0.25 M 6 x 450 ml 1 x 450 ml 6 x 180 ml 6 x 450 ml 6 x 450 ml 6 x 450 ml 6 x 180 ml 6 x 450 ml 6 x 180 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 180 ml 6 x 450 ml 6 x 180 ml 6 x 450 ml

Activator 42
Catalog No.
L0302502-04 L3300182-06 L8300452-06 L8300202-06 L0302512-04 L8300462-06 L8300212-06

Description
Activator 42 0.1 M Activator 42 0.1 M Activator 42 0.1 M Activator 42 0.1 M Activator 42 0.25 M Activator 42 0.25 M Activator 42 0.25 M

Unit
4 x 2.5 L 6 x 180 ml 6 x 450 ml 6 x 200 ml 4 x 2.5 L 6 x 450 ml 6 x 200 ml

Compatible with Expedite and Polygen Instruments L810045-06 L820090-06 L8300202-06 L8300212-06 L830045-06 L8300462-06 L840020-06 L840045-06 L850020-06 L850045-06 L860020-06 L860045-06 L870020-06 L370045-06 L880020-06 L880045-06 Acetonitrile for 8900 Instruments TCA Deblock for 8900 Instruments Activator 42 0.1 M Activator 42 0.25 M Activator 42 0.1 M Activator 42 0.25 M Cap A for 8900 Instruments Cap A for 8900 Instruments Cap B for 8900 Instruments Cap B for 8900 Instruments Oxidizer 0.02 M for 8900 Instruments Oxidizer 0.02 M for 8900 Instruments Fast Deprotection Cap A for 8900 Instruments Fast Deprotection Cap A DCI Activator 0.25 M for 8900 Instruments DCI Activator 0.25 M 6 x 450 ml 6 x 900 ml 6 x 200 ml 6 x 200 ml 6 x 450 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml 6 x 200 ml 6 x 450 ml

Compatible with Äkta and Oligo Pilot Instruments
L520251-04 L540250-01 L550250-01 L550251-01 L560250-04 DCA Deblock (Toluene) Cap A for Äkta and Oligo Pilot Cap B1 for Äkta and Oligo Pilot Cap B2 for Äkta and Oligo Pilot Oxidizer 0.05 M for Äkta and Oligo Pilot 4 x 2.5 L 1 x 2.5 L 1 x 1.25 L 1 x 1.25 L 4 x 2.5 L

60

To be inspired

SAFC Supply Solutions® Welcome to the future of genomics

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SAFC Supply Solutions® recognizes that, as products move through pharmaceutical and diagnostic development, technologies and compliance requirements evolve. With over 25 years of experience in our Hamburg manufacturing facility, we bring you Proligo® Reagents, a dedicated and comprehensive line of raw materials for oligonucleotides synthesis including DNA and RNA Pharmadites, standard and modified Amidites, Linkers & Modifiers, Supports & Solvents. Contact your local representative to learn more about our technical and quality package.

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KMI 04353 0408 SAFC Supply Solutions®, Pharmadite®, SAFC Proligo® Reagents, and Activator 42® are a registered trademarks of Sigma-Aldrich Biotechnology L.P and Sigma-Aldrich Co. . Millipore® and CPG® are U.S. registered trademarks owned by Millipore Corporation or an affiliated company. Perkin-Elmer® and Perseptive Biosystems® are registered trademarks of the Perkin-Elmer Corporation, PerSeptive Biosystems, Inc. or other subsidiaries in the U.S. and certain other countries. ABI® is a registered trademark of Applera Corporation or its subsidiaries. Expedite™ is a trademark of Applera Corporation or its subsidiaries. PolyGen® is a registered trademark of PolyGen GmbH. *LNA® / Locked Nucleic Acid® is a registered trademark of Exiqon A/S, Vedbaek, Denmark. Cy trademarks are licensed through Molecular Probes which is now fully owned by Invitrogen. All other trademarks are the property of their respective owners. Reproduction forbidden without permission. LNA oligonucleotides and phosphoramidites are sold under license from Exiqon A/S for research use only. Not for resale or for therapeutic use or use in humans. Specifications are subject to change. © 2008 SAFC All rights reserved Printed in USA

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