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16.10.09 Lab Course Crystal Growth I for MSc Crystalline Materials CRYS 412, Crystal Growth Methods I, Solution Growth
Contents
1 2 2.1 2.2 3 4 5 6
Overview............................................................................................................... 3 Preparations......................................................................................................... 5 Cleaning................................................................................................................. 5 Growth Apparatus ................................................................................................. 5 Growth of Seed Crystals ..................................................................................... 7 Preparation of the Saturated Solution............................................................... 8 Determination of the Saturation Point of the Solution .................................... 9 Crystal Growth .................................................................................................. 10 Operating Instructions of the Membrane Pump............................. 11
Appendix A:
chapter 1
title Overview
page 3 of 13
1
Task:
Overview
Crystal growth of CuSO4 5 H2O from an aqueous solution by means of the temperature reduction method
Knowledge: Phase diagrams, nucleation, growth mechanisms (e.g. Kossel crystal), growth morphology, methods of solution growth, crystal classes, and properties.
Literature: Handbook Of Crystal Growth, D.T.J. Hurle, North-Holland 1994 Crystal Growth, B.R. Pamplin, Pergamon Press 1975 Kristallzchtung, K.-Th. Wilke, J. Bohm, Verlag Harri Deutsch, Thun (Frankfurt/Main 1988)
Equipment: Fume hood, growth apparatus with control unit, bright lamp (e.g. cold-light lamp), drying cabinet with temperature control, various beakers (300 2000 ml) with appropriate cover glasses (watch glasses), crystallizing dish, heating plate with magnetic rotation, glass rod, thermometer (up to 80 C), filter paper with matching funnel, laboratory stand, clamps etc., nylon thread, latex gloves, lint-free paper towels, Teflon tweezers, plastic tins for storage.
Chemicals: 1000g copper (II) sulphate pentahydrate, several litres of 18 Mcm H2O, detergents, alcohol (e.g. isopropanol)
chapter 1
title Overview
page 4 of 13
Hazards: Copper (II) sulphate pentahydrate is harmful if swallowed and irritates eyes and skin. It is also very toxic to aquatic organisms and therefore may not go down the sink. For your own protection and to avoid contamination of the growth solution always wear latex gloves. For detailed information see the material safety data sheet .
Finally the growth apparatus has to be labelled, because it cant be supervised all the time. The label should contain the following informations: Experiment title, possible dangers, what to do in emergency case, and contacts of the responsible persons (Tel.-Nr., ).
chapter 2
title Preparations
page 5 of 13
2
2.1
Preparations
Cleaning
Solid particles (dust, lint, etc.) on the devices or in the solution can lead to parasitic nucleation. These parasite nuclei often occur at advanced stages of the growth process and destroy the previous work. Therefore: Thoroughly clean all equipment parts: a) Flushing with soap water (you can also use available detergents) and plenty of tab water b) Repeatedly rinsing with 18 Mcm H2O (wash bottle) c) Finally washing down with deionised H2O d) Dripping or drying (drying cabinet) Warning: Do not heat Plexiglas over 50 C!!! Keep receptacles, empty or with solution, always covered (use cover classes of corresponding size, especially under the fume hood).
2.2
Growth Apparatus
The growth apparatus (see Fig. 1 and Fig. 2) should be prepared as follows: Fill the outer vessel up to 1-2 cm under the aluminium ring with H2O (it may be possible that it has to be replenished after some days). Start the pump and set the thermostat (contact thermometer) so that the water bath gets the chosen temperature. Thoroughly clean all parts of the crystallizing vessel and the stirring unit (with detergent) and rinse them several times (first with tab water, then with 18 Mcm H2O). Let the individual parts drain and do not dry it with cloth. Assemble the equipment immediately, so that no dust from the air can get into the crystallizing vessel.
chapter 2
title Preparations
page 6 of 13
Fig. 1 Overview of the growth apparatus for solution growth (here with triglycine sulphate crystal) Temperature controller and circulation pump Contact thermometer as sensor for temperature changes Motor with gearbox for automatic temperature regulation Motor and gearbox of stirring unit Outer vessel of the apparatus Heated solution of the growth material Crystallizing vessel Stirring with seed holder Heated water bath (18 Mcm H2O)
chapter 3
page 7 of 13
While the growth apparatus gets a uniform temperature, a solution for the growth of seed crystals should be prepared: Prepare 30 g CuSO4 5 H2O with 100 ml 18 Mcm H2O in a 300 ml cooking cup. Dissolve the CuSO4 5 H2O at about 50 C under stirring (on heating plate). If a residue remains one can a) add 18 Mcm H2O, b) heat a little more, or c) filter it out. Let it stay over night at room temperature (there should be some crystals formed, see Fig. 3). Filter the remaining solution over the crystals into a crystallizing dish. Place six to ten seeds (single-crystals, about 5-10 mm in size) out of the sediment into a crystallizing dish (set them in approximately equal distances). Tie a fishing line (about 20 cm in length) to the obtained seeds.
chapter 4
page 8 of 13
The following steps have to be performed: Clean up the flask (3 l), suction filter, and funnel, then heat it to about 85 C in the drying cabinet. Attention: Do not heat the sealing cuffs!!! Choose desired temperature (50-60 C), at which you want to start your growth. On the basis of the solubility diagram of CuSO4 5 H2O (see Appendix B) calculate the mass you need to saturate 1000 ml H2O at the chosen temperature. Weigh the calculated mass of CuSO4 5 H2O and do it in a cooking cup. Add 1000 ml H2O and dissolve at 60 C on the heating plate with a Teflon stirrer. During the dissolution (under flue) cover the cooking cup with a watch glass. Filter the prepared solution into the heated glass equipment (feeding bottle) with the membrane pump (for operating instructions see Appendix A). Fill the filtered solution quickly into the crystallizing vessel of the growth apparatus (put the funnel into the big opening of the Plexiglas cover). Attention: Do not spill anything! Risk of contamination of the solution by parasitic seeds!
chapter 5
page 9 of 13
A seed crystal with cord serves as a solid-state sensor for the streaking method. Perform the following steps quickly and with latex gloves to avoid further contamination of the solution: Clean seed and cord carefully with alcohol and dip it briefly into deionised H2O to roughen the crystal surface. Fasten the seed with Teflon tweezers to the Teflon screw of the mixer and close the crystallizing vessel. Observe the seed crystal in the back light of a lamp. Three cases are possible (see Fig. 4): a) Streaking down b) Streaking up c) No streaking Seed crystal dissolves
Seed crystal grows Saturation
If the temperature must be corrected, the seed crystal should be removed from the solution and be cleaned every time. Alternatively the saturation point may also be determined with a seed crystal of lesser quality.
chapter 6
page 10 of 13
Crystal Growth
If the saturation point has been reached (temperature can vary from the chosen one), adjust the temperature reducer to 1 (Attention: Adjust the switch to Temperatur reduzieren!). Now observe the growth apparatus for some days and if possible, dont touch it. It will probably come to parasitic seed crystal growth, which means further crystals are being formed in the crystallization vessel. As long as they dont interfere with the growth of the crystal at the Nylon cord the growth can be continued. Otherwise a new (cleaner) saturated solution has to be prepared. Over the whole time an experiment log has to be written. After the growth, raise the crystal over the solution and switch off the thermostat. Do not remove the crystal from the crystallization vessel, but let it cool down over night. On the next day, weigh the crystal and control the mass. Switch off the thermostat, dispose the remaining solution together with the supervisor, clean up the growth apparatus, the working place, as well as all equipment used, and leave all in good condition.
chapter
title Appendix A:
page 11 of 13
Fig. 5 Membrane Pump Turning on the pump and measurement system: The main switch to turn on the measurement system and the pump is located on the back of the pump (only the main switch of the pump!). After pushing the main power switch, the pump is switched on by pushing the second switch (black) located on the left front side of the pump. The diaphragm pump offers two ways of evacuation: 1.) Ongoing pumping without regulation (on the display the arrows can`t be seen) 2.) Pumping with the closure of a magnetic valve at a previously defined pressure
chapter
title Appendix A:
page 12 of 13
(Attention: The valve closes when the previously set pressure is reached, the achieved under-pressure in the system is lower!) On the display is shown. Set each mode using the button MODE .
Setting the parameters for pumping with magnetic valve: Setting the lower limit (opening of the magnetic valve) using the buttons During the programming SET P appears on the display. Define the upper limit of the pressure region using the keys
P.
P.
During the programming SET P appears on the display. Above the pressure P + P the magnetic valve opens. Located above the magnetic valve is a manually operated gas valve, which allows a defined air entrance into the system and to the pump. It is irrelevant whether the magnetic valve is open or not. This can prevent a too strong pumping at the opening of the magnetic valve. After setting the working parameters, the pumping can be started or ended by using the button
START . STOP
After pumping the system can be aerated using the button VENT . Display in operation:
Magnetic valve is closed.
chapter
title Appendix B:
page 13 of 13
2,2 2,0
[08.10.2009 16:00 "/Graph1" (2455112)] Polynomielle Regression fr Data1_Solubility: Y = A + B1*X + B2*X^2 + B3*X^3 + B4*X^4 Parameter Wert Fehler -----------------------------------------------------------A 0,2453 0,00129 B1 0,00288 1,90882E-4 B2 1,64041E-4 8,23292E-6 B3 -2,34216E-6 1,30536E-7 B4 2,41153E-8 6,8154E-10 -----------------------------------------------------------R-Square(COD) SD N P -----------------------------------------------------------0,99997 0,0027 96 <0.0001 ------------------------------------------------------------
1,8 1,6 1,4 1,2 1,0 0,8 0,6 0,4 0,2 0 20 40 60 80 100
Data source: Physical Science Data 6 Solubility In Inorganic Two-Component Systems M. Broul, J. Nyvlt, O. S hnel Elsevier 1981
Temperature [C]
Toxicological Data on Ingredients: Copper sulfate pentahydrate: ORAL (LD50): Acute: 300 mg/kg [Rat.]. DERMAL (LD50): Acute: >2000 mg/kg [Rat].
p. 1
Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Cold water may be used. Get medical attention. Skin Contact: In case of contact, immediately flush skin with plenty of water. Cover the irritated skin with an emollient. Remove contaminated clothing and shoes. Cold water may be used.Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention. Serious Skin Contact: Wash with a disinfectant soap and cover the contaminated skin with an anti-bacterial cream. Seek immediate medical attention. Inhalation: If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention. Serious Inhalation: Not available. Ingestion: Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. If large quantities of this material are swallowed, call a physician immediately. Loosen tight clothing such as a collar, tie, belt or waistband. Serious Ingestion: Not available.
present at a concentration level above TLV. Check TLV on the MSDS and with local authorities.
Odor Threshold: Not available. Water/Oil Dist. Coeff.: Not available. Ionicity (in Water): Not available. Dispersion Properties: See solubility in water, methanol. Solubility: Easily soluble in hot water. Soluble in cold water, methanol. Solubility in water: 31.6 g/100 ml @ 0 deg. C.; 203.3 g/100 ml @ 100 deg. C Solubility in methanol: 15.6 g/100 ml @ 18 deg. C. Insoluble in ethanol. It readily forms alkaline complexes at sufficiently high concentrations of amines or alkali cyanides. Practically insoluble in most organic solvents.
p. 4
Special Remarks on other Toxic Effects on Humans: Acute Potential Health Effects: Skin: Causes skin irritation. May cause skin burns. It may cause and itching allergic eczema. Eyes: Causes eye irritation. May cause eye burns. It may cause conjunctivitis, corneal discoloration, ulceration and turbidity of the cornea. Inhalation: Causes respiratory tract (nose, throat, lung) irritation with coughing and wheezing. May cause ulceration and perforation of the nasal septum if inhaled in excessive quantities. Burning copper sulfate may result in irritating and poisonous gases which may irritate the respiratory tract and lungs, and may cause fume metal fever which is characterized by flu-like symptoms such as fever, chills, muscle aches. Ingestion: Harmful if swallowed. May cause gastrointestinal tract irritation with nausea, vomiting, diarrhea, metallic taste, burning sensation in the stomach or epigastrum, abdominal pain, and possible gastrointestinal tract bleeding. May affect metabolism(metabolic acidosis), liver (liver damage, jaundice), blood (Methemoglobin, hemalytic anemia), urinary system (kidney damage, hematuria, hemoglobinuria, albuminuria), behavior/nervous systems (somnolence, tremor, psychosis, muscle weakness, coma), cardiovascular system (lowering of blood pressure, dysthrythmia). Oral mucosa, vomitus, stools, and saliva may be stained blue or green following ingestion. Aspiration pneumonia may develop following emesis and CNS depression. Chronic Potential Health Effects: Skin: Repeated or prolonged skin contact may cause thickening of the skin.
p. 5
Identification: : Environmentally hazardous substance, n.o.s. (Cupric Sulfate) UNNA: 3077 PG: III Special Provisions for Transport: additional markings "Marine Pollutant" - required for bulk shipments. The words "Marine Pollutant" must be entered on the shipping paper in association iwth the basic DOT description for bulk shipments.
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