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Fluorescent Dye Properties

Fluorochrome Excitation (nm) Emission (nm) Color Application
Fluorescent Dye Properties
526 (DNA) Green(DNA) Nucleic acid stain. Flow cytometry, fluorescence
Fluorochrome
Acridine Orange Excitation
500 (nm) Emission (nm) Color Application
650 (RNA) Red (RNA) microscopy
Cy3 552 526 (DNA)
568-574 Green(DNA)
Red-orange Nucleic acid stain. Flow
Protein conjugation. Flowcytometry,
cytometry. fluorescence
Acridine Orange 500
Cy5 649 650670
(RNA) Red (RNA) microscopy
Protein conjugation. Fluorescence microscopy.
Cy3 552 568-574 Red-orange Protein conjugation.
AT, mimor groove dsDNA Flow cytometry.
stain. Flow
DAPI
Cy5 360
649 450
670 Blue
Red Protein conjugation. Fluorescence microscopy.
cyctometry, fluorescence microscopy.
AT, mimor groove
Membrane dsDNA-stain.
stain (ER,CM) Flow
cell fusion/
DAPI
Dil-C18-(3) 360
550 450
565 Blue
Red-orange cyctometry, fluorescence
permeabilization. microscopy.
Flow cytometry
Ethidium Bromide 493 620 Red Membrane
Nucleic acidstain
stain.(ER,CM) - cell fusion/
Gel electrophoresis.
Dil-C18-(3) 550 565 Red-orange
permeabilization.
Protein conjugation. Flow cytometry
Flow cytometry,
Fluorescein (FITC)
Ethidium Bromide 495
493 525
620 Green
Red Nucleic acid stain. Gel electrophoresis.
fluorescence microscopy.
Green Fluorescent Proteinprotein.
Fusion conjugation. Flow cytometry,
Recombinant protein
Fluorescein (FITC) 395495
(470) 525
509 Green
Protein (GFP) fluorescenceFluorescence
expression. microscopy. microscopy.
Green Fluorescent Fusion
AT, protein.
minor groove Recombinant
dsDNA stain. protein
Flow cytometry,
Hoeschst 33258 395360
(470) 509
470 Green
Blue
Protein (GFP) expression. Fluorescence
fluorescence microscopy microscopy.
R-Phycoerythrin AT, minor groove dsDNA Flow stain. Flow cytometry,
Hoeschst 33258 (PE) 488
360 578
470 Red-orange
Blue Protein conjugation. cytometry.
fluorescence
Cell membrane microscopy
stain-cell tracking. Flow
PKH2
R-Phycoerythrin (PE) 490
488 504
578 Green
Red-orange Protein conjugation. Flow cytometry.
cytometry, fluorescence micrscopy
Cell membrane stain-cell tracking. Flow
strain-celltracking.
PKH2
PKH26 490
551 504
567 Green
Red-orange cytometry, fluorescence micrscopy
microscopy.
Cell membrane strain-celltracking.
stain-cell tracking. Flow
PKH26
PKH67 551
490 567
502 Red-orange
Green cytometry, fluorescence microscopy.
Cell membrane
Nucleic stain-cell
acid stain. tracking. fluorescence
tracking,
Flow cytometry Flow
CellVue® Claret
PKH67
Propidium Iodide 655
490
493 675
502
632 Far-Red
Green
Red cytometry, fluorescense
cytometry,
microscopy. fluorescence microscopy.
microscopy.
Nucleic acid stain. Flow
Protein conjugation. cytometry
Tandem dye. fluorescence
Flow
PropidiumRed™
Quantum Iodide 493
488 632
670 Red
microscopy.
cytometry
Protein conjugation. Flow Tandem dye. Flow
cytometry,
Quantum Red™
Rhodamine (TRITC) 488
552 670
570 Red
Red-orange cytometry microscopy.
fluorescence
Protein conjugation. Flow cytometry,
Rhodamine
Texas Red (TRITC) 552
596 570
620 Red-orange
Red fluorescence microscopy.
mircoscopy.
Protein conjugation. Flow cytometry,
Texas Red 596 620 Red
fluorescence mircoscopy.