Biaffin GmbH & Co KG

Reagents, chemicals and proteins for exploring cellular signalling networks

Rosmarinic acid
1. Description
Rosmarinic acid is natural occurring polyphenolic compound consisting of an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid. It is commonly found in several taxonomically non-related families like Boraginaceae, Blechnaceae and Nepetoideae. Many biological functions of rosmarinic acid are discussed e.g. antioxidative, antiinflammatory, antimutagen, antibacterial and antiviral activities. Recent studies shows that endothelial cells pretreated with rosmarinic acid are insensitive to H2O2 induced interleukin-8 and VEGF mediated angiogenic processes. Additionally, rosmarinic acid prevents amyloid--peptide induced neurotoxicity by reduction of reactive oxygen formation and inhibits the formation of fibrils of amyloid- peptides and destabilized preformed fibrils in vitro. Rosmarinic acid inhibits Fyn tyrosine kinase activity (IC50 1.3 M) in a mixed mechanism and induces apoptosis in T-cells through the mitochondrial pathway in an Lck kinasedependent manner.

2. Product Information
Myricetin: Synonyms: Chemical name.: order-no. PKI-ROS Rosmarinate, Rosmarimic acid, (R)-rosmarinic acid [(R)-a-[[3-(3,4-Dihydroxyphenyl)-1-oxo-2E-propenyl]oxy]3,4-dihydroxy-benzenepropanoic acid] Formula: Molecular Weight: Long Term Storage: Purity: Appearance: Solubility: C18H16O8 360.3 g/mol -20 C >98% white to brown solid Soluble in 100% ethanol, DMSO (up to 25 mg/ml); also soluble in PBS (up to 15 mg/ml)
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Biaffin GmbH & Co KG Tel. +49 (0)561-804 4662 CEO: Dr. Bastian Zimmermann Heinrich-Plett-Strasse 40 Fax +49 (0) 561-804 4665 Banking account, Deutsche Bank PGK AG D-34132 Kassel, Germany E-Mail: info@proteinkinase.de IBAN DE03 4307 0024 0102 3340 00 HRA 16107, Amtsgericht Kassel Internet: www.proteinkinase.de VAT-ID No. DE215481967

Biaffin GmbH & Co KG

Reagents, chemicals and proteins for exploring cellular signalling networks

Chemical Structure:

3. Product specific literature references:

Petersen M, Simmonds MS. (2003) "Rosmarinic acid."Phytochemistry. 62(2):121-5. Huang SS, Zheng RL.(2006) "Rosmarinic acid inhibits angiogenesis and its mechanism of action in vitro." Cancer Lett. 8;239(2):271-80. Iuvone, T.; De Filippis, D.; Esposito, G.; DAmico, A.; Izzo, A. A.(2006)"The Spice Sage and Its Active Ingredient Rosmarinic Acid Protect PC12 Cells from Amyloid-beta Peptide-Induced Neurotoxicity." J.Pharmacol. Exp. Ther. 317 (3), 1143-1149. Ono K, Hasegawa K, Naiki H, and Yamada M (2004) "Curcumin has potent antiamyloidogenic effects for Alzheimer's beta-amyloid fibrils in vitro." J Neurosci Res 15: 742750. Jeli D, Mildner B, Kostrun S, Nuji K, Verbanac D, Culi O, Antolovi R, Brandt W. (2007) "Homology modeling of human Fyn kinase structure: discovery of rosmarinic acid as a new Fyn kinase inhibitor and in silico study of its possible binding modes." J Med Chem. 22;50(6):1090100. Hur, Y-G.; Yun, Y.; Won, J. (2004) Rosmarinic Acid Induces p56lck-Dependent Apoptosis in Jurkat and Peripheral T Cells via Mitochondria Pathway Independent from Fas/Fas Ligand Interaction. J.Immunol. 172, 79-87.

The sample is for non-clinical in vitro research use only and has not been approved for any other purposes including, without limitation, diagnostic or therapeutic purposes, and use in preparation of food or pharmaceutical products or for administration to humans or animals.
Biaffin GmbH & Co KG Tel. +49 (0)561-804 4662 CEO: Dr. Bastian Zimmermann Heinrich-Plett-Strasse 40 Fax +49 (0) 561-804 4665 Banking account, Deutsche Bank PGK AG D-34132 Kassel, Germany E-Mail: info@proteinkinase.de IBAN DE03 4307 0024 0102 3340 00 HRA 16107, Amtsgericht Kassel Internet: www.proteinkinase.de VAT-ID No. DE215481967

Biaffin GmbH & Co KG

Reagents, chemicals and proteins for exploring cellular signalling networks

Biaffin GmbH & Co KG Tel. +49 (0)561-804 4662 CEO: Dr. Bastian Zimmermann Heinrich-Plett-Strasse 40 Fax +49 (0) 561-804 4665 Banking account, Deutsche Bank PGK AG D-34132 Kassel, Germany E-Mail: info@proteinkinase.de IBAN DE03 4307 0024 0102 3340 00 HRA 16107, Amtsgericht Kassel Internet: www.proteinkinase.de VAT-ID No. DE215481967