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Week 1 material revisited

(bolded and underlined text indicates corrections or additional relevant material not
previously mentioned)
Outgrowth of Koch’s Discoveries
a. Criteria for establishing a causative link between an infectious agent and a disease
b. Koch’s postulates
i. The microbe must be found in all cases of the disease, but absent from healthy
ii. The microbe must be isolated from the diseased host and grown in pure culture
iii. The microbe must cause the same disease when introduced into a healthy,
susceptible host
iv. The same strain of microbe must be obtained from the newly diseased host
Seminal events that launched the modern age (Page 12)
c. Be able to place these events in chronological order
Relevance of microbiology (Page 14)
Groups of microorganisms (Page 15)
d. Classify Prokaryotic and Eukaryotic
e. Viruses/ prions are also microbes? YES (for the purposes of this class)
f. Can microbes can be seen with naked eye?
Relationship of microbes to other forms of life (Page 16)
g. Similarities
i. Metabolism
1. glycolysis
ii. Physical structure
1. What is the accepted model for both eukaryotic and prokaryotic membranes and who
created this model?
2. Describe the fluid mosaic model.
h. Differences
i. Size
1. more complex tends to be larger (euk > prok)
ii. Cellular complexity
1. cellular organization, organisms such as plants, animals, insects, fungi, etc. are much
more complex than your typical bacterium (euk > prok)
iii. Metabolic diversity
1. prok > euk
Function of membrane enclosed systems (Page 17)
Presence or Absence of sterols * not in reader
i. Eukaryotes
i. present
j. Most prokaryotes
i. absent
k. Exceptions
i. Mycoplasma
ii. Methanotrophs
Week 2 material
Define infection (Not in reader)
a. The growth of a ________ in or on a host
Ribosomal subunits (Page 18)
Significance of subunit type (Page 19)
b. What can cycloheximide be used for?
Peroxisome function (Not in text nor reader)
c. What can the peroxisome do?
d. What is the enzyme located in the peroxisome that is involved in the function of the
Surface area to volume ratio (Not in reader)
e. Small cells: ________ SA/V ratio
i. (Greater/ Less) efficient trafficking
ii. Results in (faster/ slower) growth rate
f. Large cells: ________ SA/V ratio
i. (Greater/ Less) efficient trafficking
ii. Results in (faster/ slower) growth rate
Metabolic Diversity (Page 22)
g. Similarities
h. Differences
Cytoskeletal elements (Not in reader)
i. Microfilaments:
j. Intermediate filaments:
k. Microtubules:
l. Do both eukaryotes and prokaryotes have cytoskeletal elements?
Nuclear apparatus and sex (Page 23)
Arrangement of cocci (Page 30)
m. Staphylococcus vs Sarcinae
i. Staphylococcus:
ii. Sarcinae:
Morphological properties of bacteria (Page 27)
n. ________, ________, ________
Comparison of gram positive and gram negative characteristics (Page 31)
o. Capsule vs slime layer (Page 34)
p. Composition of capsules and slime layers (Page 35)
q. Components (Pages 36-37)
r. Representative bacteria with capsule as major virulence factor (Page 40)
s. S-layer (Page 41)
Flagella and pilli (Page 43)
Flagella arrangements (Page 47)
Principles of chemotaxis (Page 49)
t. Run with flagella rotating in ________ direction
u. Tumble with flagella rotating in ________ direction
Week 3 material
Lecture 5
Peptidoglycan special features (Page 51)
Major types of peptidoglycan (Page 55)
Peptidoglycan generalizations (Page 61)
Stages of murein biosynthesis (Page 64)
Biological activities of muramyl peptides (Page 62)
Lecture 6
Inhibition of peptidoglycan synthesis
a. Phosphomycin
b. D-cycloserine
c. Bacitracin
Transpeptidation inhibition
d. Penicillin
e. Vancomycin
Murein hydrolases (autolysins)
Teichoic acidsWeek 5-6 material
Molecular chaperones (Page 81).
Inclusion bodies (Page 85-86)
Bacterial Endospore Structure (Page 90)
Properties of endospore core (Page 92)
Type III secretion system (Page 88)
Type IV secretion system (Page 89)
Microbial growth (Types)
Stages of growth (Page 101)
Nutrients (Page 109)
Nutritional categories of organisms (Page 110)
MacConkey agar (Page 112)
Blood agar media (Page 113)
Oxygen reactive species (Page 116)
Anaerobic environments (Page 117)
Notes on temperature categories (Page 118)
General conclusions on upper temperature limits for growth (Page 119)

BIMM 120 Winter 2009 TA: Abraham Tang

Section: Mondays 6PM; Thursdays 8AM abraham.h.tang@gmail.com
OH: by appointment