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Summary
EX-CELLTM MDCK is a serum-free medium developed for
adherent MDCK cell culture. A previous report1 illustrated
the adaptation, growth and virus production characteristics
of adherent MDCK cells in EX-CELLTM MDCK medium. The
current study sought to determine if MDCK cells could be
adapted to grow in suspension culture in EX-CELLTM MDCK
and determine whether or not the cells would be susceptible
to virus infection.
Figure 1
120.0
Attached MDCK
100.0
Suspension MDCK
80.0
Doubling Time (Hours)
60.0
40.0
20.0
0.0
1 2 3 4 5 6 7 8
Passage Num ber
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Figure 2
3.50E+06 100.0
95.0
3.00E+06
90.0
Culture Viabiltity
80.0
(Dashed Line)
(Solid Bars)
2.00E+06
75.0
1.50E+06
70.0
1.00E+06 65.0
60.0
5.00E+05
55.0
0.00E+00 50.0
1 2 3 4 5 6 7 8
Passage Number
Figure 3
6.00E+06 120
5.00E+06 100
Viable Cell Density (Cells/mL)
4.00E+06 80
Culture Viability
(Dashed Line)
(Solid Line)
3.00E+06 60
2.00E+06 40
1.00E+06 20
0.00E+00 0
0 1 2 3 4 5 6 7 8 9
Days in Culture
Figure 4
1.0E+10
MOI = 0.01
1.0E+09 MOI = 0.1
1.0E+08
1.0E+07
1.0E+06
TCID50/mL
1.0E+05
1.0E+04
1.0E+03
1.0E+02
1.0E+01
1.0E+00
24 48 72 96 120
Hours Post-Infection
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Additional Terms and Conditions are contained in the product Catalog, a copy of which is available upon request.
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