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The Effects of Carbon Dioxide on the Rate of Photosynthesis INTRODUCTION: Carbon dioxide is a necessary reactant in the process of photosynthesis.

This activity is designed to determine the effect, if any, of different amounts of carbon dioxide on the rate of photosynthesis. Sodium bicarbonate (NaHCO3) solution will be used as your source of carbon dioxide. PURPOSE: To determine the effect of different amounts of carbon dioxide on the rate of photosynthesis. MATERIALS large test tube glass tubing with rubber tube water sodium bicarbonate solution (10%) scalpel or razor blade timer water plant test tube clamp lamp tape retort stand graduated cylinder test tube stand

PROTOCOL 1) Obtain a sprig of the water plant. Remove several leaves from around the cut end of the stem. Slice off a small portion of the stem at a 45 angle and lightly crush the end of the stem. 2) Carefully slip the glass tube over the end of the stem and place the plant in the test tube with the appropriate solution. 3) Draw water up into the glass tube by placing the rubber tubing in your mouth and gently sucking. Clamp the rubber tubing to hold the water column in place at least half way up the tubing. 4) Secure the test tube and glass tube to the retort stand with a test tube clamp and tape 5) Measure out the volume of solution needed for each test. Add the following amounts of 10%NaHCO3 to the test tubes as follows: Test tube # Final concentration of NaHCO3 Volume of solution (mL) 1 0% 50mL H2O, 0mL NaHCO3 solution 2 1% 45mL H2O, 5mL NaHCO3 solution 3 2% 40mL H2O, 10mL NaHCO3 solution 4 3% 35mL H2O, 15mL NaHCO3 solution 5 4% 30mL H2O, 20mL NaHCO3 solution 6 5% 25mL H2O, 25mL NaHCO3 solution 7 6% 20mL H2O, 30mL NaHCO3 solution 8 7% 15mL H2O, 35mL NaHCO3 solution 9 8% 10mL H2O, 40mL NaHCO3 solution 10 9% 5mL H2O, 45mL NaHCO3 solution 11 10% 0mL H2O, 50mL NaHCO3 solution 6) Top up the test tube to within 2cm of the top. 7) Place the lamp as close to the test tube as possible. After several minutes, count and record the number of oxygen bubbles rising from the cut end of the stem. Count bubbles formed for 2 minutes. If bubbles fail to appear, cut off more of the stem and then recrush. Repeat for each of the test tubes, gradually increasing the NaHCO3 amount for each trial. 8) Record your results (Be sure to return the water plant to the front of the classroom)

The Lab Report The following will give you SOME idea as to how the body of your lab report should look. This is by NO means the only items to be included. Introduction: Background Knowledge Brief description of photosynthesis and overall equation Role of carbon dioxide in photosynthesis Indicator of photosynthesis as related to the lab. Purpose: ____________________________________________________________________________

Hypothesis: _________________________________________________________________________ Results: Table of results Graph of results Summary of the trends (using slope.as an example) Discussion: Explain trends in the graph Mechanisms involved

RESULTS Tube # Percent NaHCO3 used (%) Number of Oxygen Bubbles Evolved per Minute 1 0 3 2 1 8 3 2 15 4 3 23 5 4 33 6 5 50 7 6 52 8 7 53 9 8 53 10 9 52 11 10 53