6X DNA Loading Dye 6X Orange DNA Loading Dye

Description 6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases. 6X Orange DNA Loading Dye is used for conventional DNA electrophoresis (seerecommendations for use). Quality Control Tested for DNA sample preparation prior to agarose gel electrophoresis. The absence of deoxyribonucleases confirmed by appropriate tests. Composition

10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol, 60 mM EDTA Storage Store at room temperature or at 4C up to 12 months. For longer periods, store at -20C.

6X Bromophenol blue/Xylene cyanol DNA Loading Dye

Description 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solution binds divalent metal ions and inhibits metal-dependent nucleases. 6X DNA Loading Dye is used for conventional DNA electrophoresis (see recommendations for use). Quality Control Tested for DNA sample preparation prior to agarose gel electrophoresis. The absence of deoxyribonucleases confirmed by appropriate tests. Composition

10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60 mM EDTA Storage Store at room temperature or at 4C up to 12 months. For longer periods, store at -20C.

Loading Buffer with GelRed for Electrophoresis

To do this buffer you can use either Bromophenol blue or Xylene Cyanol buffer described above. This protocol will make 5ml of 6x solution. Adjust to your needs if necessary.

1.

5 ml Loading Buffer (Xylene-cyanol or Bromophenol Blue)

2.

15 ul of GelRed (10000x in water, cat.41003 Biotium)

1 Kb Ladder with GelRed for Electrophoresis

Use the Fermentas GeneRuler 1kb ladder from the stock. Mix:

1. 2. 3.

100l (1 tube) DNA Ladder 100l 6X Loading Dye Solution with GelRed (from above) 400l Deionized water

Good to go. Load 3 to 5 l.