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ABSTRACT
pBX6 is a recombinant plasmid containing a 3 kb fragment from Fibrobacter succinogenes S 85 genomic DNA encodes xylanase
activity which express in E. coli HB 101. Growth characteristics of the plasmid showed that xylanase activity was observed after 24 to
48 h incubation period. The growth kinetic of the recombinant plasmid pBX6 on 2 Yeast Tryphtone (YT) medium was higher in the
presence of ampicylin. pBX6 produced higher xylanase activity at pH 7.4 and 37oC.
well in the media containing of glucose compare without enzyme activity at temperature of 37oC (specific activity
supplementation of this substance; however, the pBX6 119 U/mg), and at pH 7.4 (specific activity 61.99 U/mg),
failed to grow on minimal media (Figure 1). respectively.
The action of crude specific activity (U/mg) was
characterized by rapid decrease in the viscosity of the xylan
Effect of media on growth of pBX6
solution and relatively slow increase in the amount of
0.9 reducing sugar released. It was previously shown that total
0.8 endoxylanase activity of F. succinogenes S 85 culture was
optical density (OD) 550nm
70
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ACKNOWLEDGEMENT Woodward, J. 1984. Xylanases: functions, properties and applications In
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Science and Technology Malaysia to Prof. Abdullah Sipat distinct B. circulans xylanase by molecular cloning of the genes and
funded this research. The first author was grateful to ARM expression in E. coli. Applied Environmental Microbiology 55 (3):
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II Project-AARD, Indonesia for supporting the study.