CAT.

INIST
Titre du document / Document title
Enhanced inhibitory effects of hyperthermia combined with ascorbic acid on DNA synthesis in Ehrlich
ascites tumor cells grown at a low cell density
Auteur(s) / Author(s)
KAGEYAMA K. (1) ; ONOYAMA Y. ; OTANI S. ; MATSUI-YUASA I. ; NAGAO N. ; MIWA N. ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Osaka city univ. medical school, radioisotope cent., Osaka 545, JAPON

Résumé / Abstract
Effects of hyperthermia and cell densities on inhibitory activity of ascorbic acid on DNA synthesis in
Ehrlich ascites tumor cells were studied. When cells at a low density of 5 x 103/ml were treated with
75 μ M ascorbic acid for 1 h, DNA synthesis was inhibited after treatment at 37°C and the inhibition
was significantly enhanced at 42°C. At a cell density as high as 1 x 105/ml, however, inhibition did
not occur at 37°C or 42°C. In contrast, dehydroascorbic acid was inactive even at a low cell density
under similar conditions. Inhibitory effects of ascorbic acid on DNA synthesis were also markedly
enhanced by treatment at 40°C. DNA synthesis was not inhibited in the absence of the drug.
Futhermore, mice transplanted with cells treated with a combination of 75 μ M ascorbic acid and
hyperthermia at 42°C, considerably prolonged their survival time in comparison with untreated cells.
Addition of ascorbic acid to hyperthermia is suggested to be an advantageous treatment for cancer
Revue / Journal Title
Cancer biochemistry biophysics ISSN 0305-7232 CODEN CABCD4
Source / Source
1995, vol. 14, no4, pp. 273-280 (22 ref.)
Langue / Language
Anglais
Editeur / Publisher
Gordon and Breach, London, ROYAUME-UNI (1974-1999) (Revue)
Mots-clés anglais / English Keywords
Ehrlich ascites tumor ; Ascites ; Animal ; Ascorbic acid ; Chemotherapy ; Combined treatment ;
Induced hyperthermia ; In vitro ; In vivo ; Cell culture ; Cell density ; Biological activity ; DNA ;
Biosynthesis ; Cell proliferation ; Spontaneous ; Mouse ; Vitamin ; Rodentia ; Mammalia ; Vertebrata ;
Mammary gland diseases ; Malignant tumor ; Adenocarcinoma ;
Mots-clés français / French Keywords
Tumeur Ehrlich ; Ascite ; Animal ; Ascorbique acide ; Chimiothérapie ; Traitement associé ;
Hyperthermie provoquée ; In vitro ; In vivo ; Culture cellulaire ; Densité cellulaire ; Activité biologique
; DNA ; Biosynthèse ; Multiplication cellulaire ; Spontané ; Souris ; Vitamine ; Rodentia ; Mammalia ;
Vertebrata ; Glande mammaire pathologie ; Tumeur maligne ; Epithélioma glandulaire ;
Mots-clés espagnols / Spanish Keywords
Tumor Ehrlich ; Ascitis ; Animal ; Ascórbico ácido ; Quimioterapia ; Tratamiento asociado ;
Hipertermia provocada ; In vitro ; In vivo ; Cultivo celular ; Densidad celular ; Actividad biológica ;
DNA ; Biosíntesis ; Multiplicación celular ; Espontáneo ; Ratón ; Vitamina ; Rodentia ; Mammalia ;
Vertebrata ; Glándula mamaria patología ; Tumor maligno ; Adenocarcinoma ;
 Localisation / Location
INIST-CNRS, Cote INIST : 16794, 35400005933339.0060

Nº notice refdoc (ud4) : 3471870

Rechercher dans CAT.INIST / Search in CAT.INIST

Custom Search