Effect of coconut water extract on the growth and serum biochemical values of rats fed fish oil diet

Abstract To justify the safety of ethanolic extract of coconut water ( CWE) , a study with rats was conducted. Wistar rats fed fish oil diet and rats fed the standard diet of AIN- 93G groups orally administrated 0.8 ml CWE/ rate twice per day for 14 days. During experimental period, the behavior and growth of rats were monitored. At the end of experiment, the rats were anaesthetized and he internal organ were taken and measured for each weight. Blood was taken from the hearth and then analyzed for serum biochemical values and TBARS . the results, no adverse effects of CWE administration were observed as evaluated by growth pattern, food intake and feces appearance, serum biochemical values and weight of to those of untreated control. Serum TBARS value of rats fed fish oil was significantly higher than that of those fed standard diet, however, oral administration of CWE did not significantly decrease serum TBARS.

A time- course study of interactions between vitamin biphenyls in rats Abstract

A and polybrominated

The first objective of this study was to compare serum and hepatic vitamin A content in young rats as affected by the interaction the between dietary levels of vitamin A and polybrominated biphenyls (PBB) Second objective was to determine the pathogenesis of previously described histologic lesions related to combined effects of vitamin A deficiency and PBB toxicosis. The third objective was to determine if vitamin A supplemented were fed for 42 days and contained 0 or 100 mg/kg of a commercial mixture of PBB for the last 28 days of the experiment. Adverse clinical signs were not abserved. PBB significantly depressed weight gain only in rats fed a vitamin A deficient diet. Vitamin A supplementation did not alter hepatic lesions caused by PBB toxicosis, serum retinol concentrations were significantly reduced in rats fed the vitamin A deficient diet containing PBB. Hyperlasia of bile ducts and squamous metaplasia of

the thyroid and salivary glands were consisten lesions in rats vitamin A deficient diet containing PBB.

Antioxidative Effect of coconut (cocos nucifera ) Water Extract on TBARS Value in Liver of Rats Fed Fish Oil Diet Abstract

Ethanolic extract of the coconut water showed antioxidant activity in the in vitro assay using an emulsified-aqueous system containing linoleic acid and B- carotene. To evaluate the possible antioxidative effect in vivo, a study with rats fed fish oil diet, was performed. Oral administration of the coconut water extract ( CWE, 340 mg/mL) didi not influence food intake, daily body weight gain nor average liver rats of rats. It was investigated that the increase of liver TBARS value of rats fed fish oil diet was significantly (P <0,01) suppressed by oral administration 0,8 ml of the coconut water extract ( CWE, 340 mg/mL, Vrat twice per day during 14 days of experimental period. Oral administration of the coconut water extract also tended to suppress the decreasing effect of fish oil diet on liver ascorbic acid. Liver total glutathione o rats fed fish oil died was significantly lower than that of rats fed AIN93G standard diet, but oral administration of the coconut water did not significantly change liver glutathione. SOD activity in liver homogenate of rats fed fish oil diet and orally administrated by coconut water extract was significantly higher than that of those orally administrated by water. The result shower that the antioxidative properties of the coconut water extract have been demonstrated by in vivo assay using rats fish oil diet