SYLLABUSCOPYFORTHIRDYEARB.

PHARM SEMSETERV PHARMACEUTICALMEDICINALCHEMISTRYI

S.No. 1. 2. Topic Physiochemicalpropertiesanddrugaction Metabolism Discussion of the following classes of drugs including, classification, chemical nomenclature, structure including stereochemistry, generic names, chemistry, physiochemical properties, SAR, metabolism, molecularmechanismofaction,introductiontorationaldevelopmentif any,oftheclassofdrugs. Chemotherapeuticagents a. Antitubercular agents PAS*, ethonamide, isonamide, pyrazinamide, ethambutol*, antitubercular antibiotics (streptomycin, rifampin,viomycin and cycloserine thefirst three onlyhighlights of structuretobediscussed). Combinationtherapy. b.Antileproticdrugsdapsone*andclofazimine Antimalarials Natural products like cinchona alkaloids (with stereochemistry and drug action) and artemisinin and its derivatives like artether and artemether and artesunate. Synthetic antimalarials suchas8aminoquiacridineseg.Primaquine,Quinolinemethanolseg mefloquine:misc,likehalofantrineandlumefantrine:DHFRinhibitors like pyrimethamine* and cycloguanil and sulfonamides like sulfodoxine,sulfadiazine*,andsulfalene.Combinationtherapy. AntifungalagentsNaturalproductslikegriseofulvin,amphotericinB and nystatin (later two only general aspects of structure related to activity) and the antifungal azoles like clotrinazole, ketoconazole, fluconazoleanditraconazole. Antibacterialagents a. Antibiotics penicillins (natural and semisynthetic penicillins likePenicillinG,PenicillinV,ampicillin*,amoxicilline*,oxacillin, nafcillin, methacillin and ampicillin prodrugslike bacampicillin and pivampicillin), cephalosporins (cephalexin, cephalothin, cefaxitin, cefuroxime, cefotaxime, cefepine and cefpirome) tetracycline,chlortetracycline,oxytetracycline,doxycyclineand miocycline and its prodrug rolitetracycline) macrolides (erythromycin,rocithromycin,azithromycinonlyhighlightsof structure to be discussed) aminopglycosides (gentamicins andneomycinsonlyhighlightsofstructuretobediscussed) Chloramphenicol. b. Sulfonamides Short, intermediate and long acting sulfonamides,sulfonamidesforophthalmicinfections,forburn 47 |P a g e

3hrs/week
Hours 4 6

3.

4.

5.

6.

10

therapy and for intestinal infections, ulcerative colitis and for reductionofbowelflora. c. Fluroquinolones like norfloxacin, ciprofloxacin*, sparfloxacin, gatifloxacin d. Oxazolidinediones

1 *indicatessynthesistobediscussed ReferenceBooks: 1. 2. 3. 4. 5. 6. WilsonandGisvoldsTextbookofOrganicMedicinalandPharmaceuticalChemistry, th 11 Ed.,Eds.JohnHBlockandJohnMBeale,LippincottWilliams&Wilkins,2004. Foyes Principles of Medicinal Chemistry, Eds., T. L. Lemke and D. A. Williams, Williams&Wilkins,Baltimore,2002. th Medicinal Chemistry, Ashutosh Kar, 4 Edition, New Age International Publishers, 2007. The Art of drug Synthesis, Eds. Douglas S Johnson and Jie Jack Li, Wiley Interscience,2007. PharmaceuticalChemistry,Vol.EDrugSynthesisEds.H.J.Roth,A.KleemanandT. Beissewenger,EllisHorwoodLtd.,1988/ TheOrganicChemistryofDrugSynthesis,DanielLednicer,Vols.1to7,Wiley.

BIOCHEMISTRYIII
S.No. 1. Topic

3hrs/week
Hours 8

DNA replicator: Details of DNA replication, difference between prokaryotes and eukaryotes, telomeres and telomerases DNA polymorphisms and single nucleotide polymorphism. Examples with DNAorinterferingwithDNAreplication. Solid phase DNA synthesis, DNA sequence (MaximGilbert method, SangerdideoxymethodandautomationofDNAsequencing) Protein Biosynthesis: Details of DNA transcription and RNA Protein, difference between prokaryote and eukaryotes, concepts of introns and exons and intron splicing, concept of posttranslational modifications(examplesofglycosylatedproteins,conjugatedproteins, insulin).Examplesofproteinsynthesisinhibitorsusedasdrugs. Solid phase peptide synthesis, Edman reaction based protein sequencinganditsautomation. Enzyme kinetics: Classification of enzymes. Effects of enzyme concentration, substrate concentration, temperature, pH on enzyme reactions. General mechanisms of enzyme catalysis acid base catalysis, oxidationreductions, proximity effects, transition state theory, etc. Michaclis Menten equation and meanings of Km and Vmax and identification of inhibition patterns via LWB and Eadic 48 |P a g e

2.

3.

Hofsteeplots.Examplesofdrugsthatenzymeinhibitors. 4. Metabolic regulation: Brief description of the following: Enzyme compartmentalization, kinetic factors, modification of enzymes for regulation, cascade systems, repression and induction of enzymes andtheirregulationviamodulationoftranscriptionandtranslation. 8

ReferenceBooks: 1. 2. 3. 4. 5.
th Lehninger, Principles of Biochemistry, 4 Ed., Eds. Nelson D. L and Cox M. M. ReplikaPressPvt.Ltd.,India,2007. rd Biochemistry,StryerL.,3 Ed.,W.H.Freeman& Co.,1988. th Harpers Biochemistry, 25 Ed., Murray R. K., Granner D. K., Mayes P. A. and RodwellV.W.,AppletonandLange,USA,2000. th Outlines of Biochemistry, 5 Ed., Eds. Conn E. Stumpf P. K., Bruening G and Doi RoyH.JohnWiley&Sons,USA,1987. th Textbook ofBiochemistry with ClinicalCorrelations, 5 Ed.,Ed.Devlin T. M.,Wiley Liss,USA,2002.

PHARMACOGNOSYI
S.No. 1. 2. Topic Origin,scopeandhistoryofPharmacognosy

3hrs/week
Hours 2 2

Literature and information about official and related text available aboutherbalsanddrugsofnaturaloriginconceptofauthenticationof crudedrugs Role of Pharmacognosy in allopathy and traditional systems of medicine, namely, ayurveda, unani, siddha, Chinese etc. and nutraceuticals,cosmeticetc. Introduction to medicinal botany with respect to barks, wood, root, fruit, seed, flower, leaves etc. Methods of classification and their significance in the study of drugs of natural origin (alphabetical, biological, chemical, taxonomical, chemataxonomical, and pharmacological)andsourcesofdrugsofnatureorigin(Plant,animal, mineralandmarinewithoneexampleofeachclass). Pharmacognosyofcrudedrugs Cultivation,collection,preparation,drying,storage,andqualitycontrol of drugs of natural origin. Commerce and trade of drugs of natural origin. Methods of extraction (percolation, maceration, soxchlet etc.) of different classesofphytochemicalsfrom crude drugs.Introduction to newertechniquesofextraction. Primary and secondary metabolites and their biosynthetic pathways. Study of terpenoids, fixed oils, shikimic acid pathway, acetate hypothesisandpolyketideswithoneexampleofeachclass. Plant cell structure with respect to cell organelles and cell contents 49 |P a g e

3.

4.

5.

6.

7.

8.

suchasstarchgrains,calciumoxalatecrystals,idoblastsetc. 9. Introductiontoplanttissuecultureanditstechniqueand applications plantgrowthregulatorsandhormones. 3

ReferenceBooks: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. TreaseD.&EvansW.C.:TextBookofPharmacognosy:W.B.Saunders. TylerV.E.BradyL.R.&RobbersJ.E.:PharmacognosyLeaFeibger,USA. WallisT.E.TextBookofPharmacognosyCBSPublishers,Delhi. Kokate C. K., Purohit A. P. & Gokhale S. B.: Pharmacognosy Nirali Publications, Pune. Harbone J. B.: Phytochemical Methods: A guide to modern techniques Analysis: Chapman&Hall,London. BrunctonJ.:Pharmacognosy,Phytochemistry,MedicinalPlants:InterceptLimited. VasudevanT.N.&LaddhaK.S.:ATextbookof Pharmacognosy,VrindaPublication House,Jalgaon. TheIndianPharmacopeia:TheControllerofPublicationDelhi. BrainK.R.&TurnerT.D.:ThePracticalEvaluationofPhytopharmaceuticals:Wright, Scientica,Bristol. IyengerM.A.&NayakS.G.:AnatomyofCrudeDrugs:ManipalPowerPressManipal. IyengerM.A.:PharmacognosyofPowderedDrugsManipalPowerPress,Manipal. KokateC.K.:PracticalPharmacognosyVallabhPrakashan. Wagner,Bladt&ZgainskiplantDrugAnalysisSpringerVerlag. Khandelwal K. R.: Practical Pharmacognosy Techniques and Experiments Nirali Prakashan,Pune. Vasudevan T. N. Laddha K. S.: Practical Pharmacognosy New Vrinda Publishing House,Jalgaon.

PHARMACEUTICSIV
S.No. 1. Topic

3hrs/week
Hours 2 3 7

Tablets DefinitionAdvantagesandLimitationsPreformulationaspects Tablet formulation and design additives excipients with examples Largescalemanufacturingwithequipmentsfordrying,mixing, Direct compression, Granulation, Dry Granulation (Slugging AndRollerCompaction) Compression(SinglestationtabletpressandRotarypress), Physicsoftabletcompression(brief),Processingproblems,in tabletting,Layoutoftabletsection. Tablet types: Effervescent, Buccal, Iozenges, chewable, sublingual, Dispersible, soluble, Orodispesible Q. C. of Tablets. Capsules 50 |P a g e

4 6

2.

HardcapsulesRawmaterials,gelatinmanufacturing(brief), manufacturing of hard capsule shells, size, sealing, storage, Mention of gelatin substitute vegetarian capsules, Hard capsule fill formulation aspects, Large scale manufacturing fillingofhardcapsuleshells,fillingequipmentswithexamples (stressorprincipleofequipments),Packagingstrip&Blister packagingequipments.Q.C.tests,Layoutofcapsulesection. Soft Gelatin Capsules Raw material Gelatin desirable properties, Soft Capsule properties, nature of shell & contents, Large scale manufacturing Rotary Die Process PackagingQ.C.tests. 5

3.

Acrosols Definition,advantages&disadvantages,desirablefeatures. Components Prepellantstypes, selection, two phase & three phase systems. Containers Tin Plate, Aluminum, Glass, Plastics, Valve, & Actuator Standard valve (detail) & specialized valves (in brief), Product concentrate Different formulationsystemssolution,Dispersions,FoamsPowders.

ReferenceBooks: 1. 2. 3. 4. 5.
nd AultonMichaelE.,PharmaceuticsTheScienceofDosageFormDesign,2 Edition, 2002,ChurchillLivingstonePublishers. LiebermanHerbertA.,LachmanLeon,Schwartz/JosephB.,PharmaceuticalDosage rd FormsTablets,Volume1/2/3,3 Edition,2005,MarcelDekkerInc.,NewYork. LachmanLeon,LiebermanHerbertA,KanigJosephL.,TheTheoryandPracticeof rd IndustrialPharmacy,3 Edition1987,VarghesePublishingHouse,Mumbai. th E.A.Rawlins,Ed.,BentleysTextbookofPharmaceutics,8 Edn,BallierweTindall, 1995. Ridgways K., Hard capsules Development & Technology, Pharmaceutical Press London,1987.

HOSPITALPHARMACYANDDRUGSTOREMANAGEMENT
S.No. 1. 2. 3. 4. 5. 6. 7. Topic

3hrs/week
Hours 1 1 2 2 2 1 2

Hospital pharmacy history and development, Duties and responsibilitiesofhospitalpharmacist. Hospitals,classification,organization,Administrationandfunctions. Pharmacy and therapeutics committee: Objective, composition and function Hospitalformulary:Advantage,disadvantage,preparation,contentsa fewexamples. Purchasingprocedure,storageandinventorycontrol Dispensingofcontrolledsubstances:Hospitalcontrolprocedures Prepackaging,manufacturingandbulkcompoundingoflargevolume 51 |P a g e

parenterals,totalparenteralnutritionandintravenousadditives. 8. Central sterile service: Advantages, plan, location, Sterilization and disposalofsurgicalmaterials.Sterilizationofrubbergloves,syringes, needles,catheters,surgicalinstruments,powdersandothermaterials. Medicalgases:Therapeuticuseofgasescolourcodingofcylinders, careofcylindersandaccessories. Health accessories: Wheel chairs, canes, crutches, bedpans, vaporizers, syringes, needles, clinical thermometers, first aid suppliers. Clinical applications of radiopharmaceuticals: Introduction to particulate radiation, half life, therapeutic and diagnostic radiopharmaceuticals,andfacilitiesrequiredprotectionofoperators. Useofcomputersinhospitals IntroductiontoPharmacyPractice PharmacyTradeorProfession ReorientationfromProducttoPatientFocus CodeofEthicsforaPharmacist CommunityPharmacy:ScopeinIndiaandAbroad Channelsofdistribution Wholesalers & Retailers and Their role. Classification, FunctionsandServices. FormsofbusinessOrganization(inbrief) Hindu Undivided family, Sole proprietorship, Partnership, Company andCooperativeSociety. Entrepreneurship: Trails of Entrepreneur and Development as an Entrepreneur Locationanalysis LayoutDesign(ExteriorandInterior) PurchasingandinventoryControl (Methods, restricted to retail only viz., Want Book, Systematic Want Book,OpentoBuyBudgeting,ABC,VEDandEOQAnalysis.Useof computersforinventorycontrol) SalesPromotion RiskManagement&InsurancePoliciesforShopkeeper FraudsinRetailPractice:Howtopreventthem 2

9. 10.

1 1

11.

12. 13.

1 1

14. 15. 16.

1 2 2

17.

18. 19. 20. 21.

1 1 1 2

22. 23. 24.

1 1 1

ReferenceBooks: 1. 2. 3. 4. 5. PrinciplesandmethodsofpharmacymanagementSmith,LeaandFebiger. DrugstoreandmanagementNolenandmaynard,McgrawHill,1941. ATextbookofpharmacymanagementTomiski(Kuganpage). DrugStoreandBusinessManagementA.P.Battase,UniquePublication. BattaseUniquePublication,1999. th Hospitalpharmacy:WilliamE.Hassan,5 Edition,Lea&Febiger,Philadelphia. 52 |P a g e

6. 7. 8.

th AtextbookofHospital:S.H.MerchantandDr.J.S.Quadry,4 Edition,B.S.Shah Prakakshan,Ahmadabad,2001. Hospital Pharmacy: Dr. H. P. Tipnis and Dr. Amrita Bajaj, First Edition, Career Publication,Maharashtra,2007. th Gennaro Alfonso R., Remington: The Science and Practice of Pharmacy, 20 Edition,2000,PublishedLippincottWilliams&Wilkins.

PHARMACEUTICALBIOTECHNOLOGY
S.No. 1. 2. 3. 4. Topic

3hrs/week
Hours 1 1 2 12

Introduction to Biotechnology, Historical Perspectives, Definitions, Scope,RelevancetoPharmaIndustry Microbiological Limit testsNeed, Standards for raw materials of naturalorigin(pharmacopoeialwithsomeexamples) MicrobiologicalassaysBasicprinciples,someexamples. Immunology Hostmicrobe interactions, Introduction to termsinfection, infestation,pathogen,resistance,susceptibilityetc. Factorsaffectingpathogenicityandinfection,organizationof immunesystemorgans&cellsinvolved. Innate defence mechanism first line of body defence, physiological phenomenainflammatory response, fever, cellular, mediators soluble (humoral) mediators, phagocytosis. Specific Defence Mechanism Characteristics, Antigen, Cellmediated immunity, Humoral immunityantibody structure and types, Pathways of immune response, Clonal selectiontheory Hypersensitivity&Allergy ImmunodeficiencystatesPrimary&acquired,Autoimmunity. Introductiontodiagnosticmarkers. Serologyprecipitin tests, agglutinin, complement fixation. Tests,immunoflueroscence,RIA,ELISA Immunological productsVaccines & Sera Definitions and Classification, Outline of general method of preparation of bacterial&viralvaccines,TypicalExamplesofeachtype,Q. C.aspects,Recenttrendsinvaccines. FermentationTechnology Example of products of fermentation (microbial, animal and plant), types of fermenters, design of fermenter, factors affecting fermentation and down stream process, Production of penicillin, dextram, amylase, Introduction to single cell protein, biological oxygendemand. IntroductiontorDNAtechnology Details of restrictions endonuclease, SI nuclease, Ligase, Alkaline phosphatase, Vectors (Plasmid, cosmid, YAC), Gene expression 53 |P a g e

5.

6.

(Bacterial expression system, Yeast expression system, animal expressionsystem,Plantexpressionsystem) Application of rDNA technology for production of Pharmaceutical productse.g.Insulin,humangrowthhormone,interferon 7. Techniquesusedinmolecularbiology Introductionto polymerase chain reaction, DNAsequencing, cDNA library,genomiclibrary,blottingtechniques,electrophoresis. Introduction to gene therapy, transgenic animal and transgenic plants,Sitedirectedmutagenesis. Definition of enzyme and cell immobilization, methods for enzyme immobilization (adsorption, covalent binding, entrapment, matrices withexample),exampleofimmobilization,introductiontobiosensor withimmobilizedenzymee.g.glucoseoxidase,penecillinase. 4

8. 9.

2 3

10. Introductionto Hybridomatechnology Production andapplication of monoclonal antibody, animal cell culture with diagnostic applications ReferenceBooks: 1. 2. 3. 4. 5. 6. 7. 8. 9.

AtextbookofbiotechnologybyR.C.Dubey. BiotechnologybyB.D.Singh. PharmaceuticalBiotechnologybyS.P.Vyasanddixit PharmaceuticalBiotechnologybyS.S.Kori. BiotechnologybyH.D.Kumar. AtextbookofmicrobiologybyAnanthnarayan. PharmaceuticalMicrobiologybyW.B.HugoandA.D.Russell. LehningerprincipleofBiochemistrybyDavid,Nelson. Pelezar, Chan & Krieg, MicrobiologyConcepts and Applications, International Edn., McGrawHill,Inc.,1993. th 10. WeirStewart:Immunology,8 Edn.,ChurchillLivingstone,1997.

PHARMACOLOGYII
S.No. 1. Topic

3hrs/week
Hours 6

2.

IntroductiontoChemotherapy Basicprinciplesofchemotherapy Mechanismofactionofchemotherapyagents Mechanismofresistancetochemotherapeuticagents Antibacterialdrugs SulfonamidesandTrimethoprim Quinolones&Fluoroquinolones Penicillins,cephalosporins&cefamycins&otherlactumantibiotics Tetracyclines Chloramphenicol Aminoglycosides Erythromycin 54 |P a g e

Macrolides 3. Chemotherapyoffollowingdiseases Amoebiasis Malaria Helminthiasis e. Tuberculosis&leprosy f. Fungalinfection g. Viraldisease h. Cancer Drugsusedinendocrinedisorders Antidiabeticagents Antithyroidagents Oxytocies Oralcontraceptives BoneMetabolism&DrugsusedinOsteoporosis (BoneStructure & Composition, Bone Remodeling,disordersof Bone andDrugsusedinTreatments) 12

4.

10

ReferenceBooks: 1. Goodman&GilmansPharmacologicalBasisofTherapeuticsJoelG.Hardman,Lee th E.Limbird,AlfredGoodmanGillMan11 Edition,TheMcGrawHillCompaniesInc., 2001. th Satoskar,R.S.BhandarkarS.D.&RegeN.N.Pharmacology&Therapeutics20 Edition,PopularPrakashan,2007. th Rang&DalePharmacology,5 Edition,ChurchillLivingstone,2003. rd Lippincotts Illustrated Reviews: Pharmacology Lippincott Raven 3 Edition Howland&NycetsPublishersNY,2006. th LewisPharmacologyByCrossland5 Edition,ChurchillLivingstone th Laurence,D.R.&BennetClinicalPharmacology9 Edition,Elsevier,N.Y.,2006. rd Kulkarni, S. K. Handbook of Experimental Pharmacology 3 Edition Vallabh PrakashanNewDelhi,2005. th B. G. Katzung Basic and Clinical Pharmacology, 9 Edition Appleton and Lange publication,2004. rd Gosh M. N. Fundamentals of Experimental Pharmacology, 3 Edition, Hilton & Company,Calcutta,2005.

2. 3. 4. 5. 6. 7. 8. 9.

PHARMACEUTICALCHEMISTRYLABORATORYI

4hrs/week

Introduction/transformationoffunctionalgroupsinmolecules 1. Acetylation(synthesisofaspirin&acetanilideorbenzanilide) 2. Bromination(synthesisofpbromoacetanilideorpnitrobromobenzene) 3. Nitration(synthesisofpnitroacetanilideormdinitrobromobenzene) 4. Oxidation(synthesisofbenzoicbyoxidationoftolueneorbenzylalcoholwithalkaline potassiumpermanganate) 5. Bromination(synthesisofsodiumtoluenepsulfonateorpNylenesulfonateacid) 55 |P a g e

6.

7.

Reduction(Ketones,synthesisofbenzhydrolbyreductionofbenzophenonewithzinc and sodium hydroxide) or synthesis of mnitroaniline by partial reduction of m dinitrobenzenewithsodiumpolysulfide. Esterification (synthesis of nbutylacetate from nbutanol and acetic acid) DemonstrationofreactionmonitoringbyTLC.

PHARMACUTICSLABORATOYIII

4hrs/week

1.A) Evaluation of excipientsbulking agent, directly compressible diluents, conventional Bulkdensity,flowproperties,compressibilityanddiscussionofobservations B) Evaluationofexcipientsdisintegratingagents Swellingindexdeterminationanddiscussionofobservations. C) EvaluationofexcipientsoftabletLubricantsglidents 2. GranulationforSolubleaspirintabletsIPandevaluation 3. GranulationcompressionandevaluationRiboflavinintabletIP96 4. Granulation,compressionandevaluationofChewableantacidtablets. 5. GranulationandCompressionofAscorbicacidtabletsIP96 6. Granulation,compressionandevaluationofParacetamoltabletsIP96 7. DissolutiontestforparacetamoltabletsIP. 8. Evaluation of Capsule shells, filling of ampicillin trihydrate capsules and their evaluation.

BIOCHEMISTRYLABORATORYII

4hrs/week

Colorimetricestimationofbloodsugar,bloodcholesterol EstimationofproteinbyBiuretmethodandFolinCiocalteaumethod EstimationofRNA EstimationofVitaminEandVitaminC Extraction of enzymes, Partial purification by alcohol, acetone precipitation, ammonium sulphate precipitation, Study of factors affecting rate of an enzymatic reactions: Effect of activators,inhibitors,orrateofenzymaticreaction,DeterminationofKm ofanyoneenzyme, Assay of alkaline phosphatase, amylase, protease, polyphenol, oxidase, lipase Chromatographicseparationofaminoacids.

PHARMACEUTICALBIOTECHNOLOGYLABORATORY
AirMicrobiologybysolidandliquidimpingementmethods ColiformCountofwaterbyMPNtechnique TestforSterilityasperIP

4hrs/week

Microbial Limit testonExcipientsasper I.P. Hard Gelatin Capsule Shells, Tragacanth, Starch,Lactose Studiesonselectivemedia:McConkeyAgar,CetrimideAgar,VogelJohnsonandMedium forStyphi 56 |P a g e

AntibioticSensitivitytestbydiscmethod Widalstesttubeagglutinationmethod BiochemicalTests(Catalase,Oxidase,Urease,Nitratase,Protease,AmylaseandIMVIC) Antimicrobialassayofantibiotic,introductiontozoneofinhibitionandcalculation Immobilizationofenzymesandcellsbycalciumalginate,gelatinandagar IsolationofDNA Selectionandisolationofbacteriabyreplicaplating Determinationofthermalofbacteriabreplicaplating EffectofUltraVioletexposureongrowthofEcoli DemonstrationofelectrophoresiseitherbyPAGEorAgarosegelelectrophoresis

57 |P a g e

SEMSETERVI PHARMACEUTICALMEDICINALCHEMISTRYII
S.No. 1. Topic SteroidsConfiguration5and5cholestane,conventionalformula andconformationalrepresentation.ReactionsinringA&Bofsteroids conformationand chemical reactivity, addition,elimination,epoxide opening, relative rates of esterification and oxidation of epimeric alcoholsandreductionofketones,rearrangementreactions,Medicinal chemistryofsteroids:Sexhormones(androgensliketestosteroneand its esters: estrogens like estradiol, ethinyl estradiol and mestranol: progestines like medroxy progesterone acetate, megesterol acetate, norethindrone and norgestrel), anabolic steroids like danazol, stanozolol and androizoxazole: non steroidal estrogens like diethylstilbestrolandcholotrianisene,antiestrogensliketamoxifenand clomiphene, corticoids and steroidal antoflammatory like cortisone, hydrocortisone, prednisolone, dexamethasone, betamethasone and triameinolone. Antihistamines H1, H2 receptors. Emphasis to be on the second generation H1 antagonists such as fexofenidine, astemazole, loratidine cetrizine and acrivastine, H2 receptor antagonist like cimetidine*, ranitidine, famotidine, nizatidine, proton pump inhibitors likeomeprazoleandlansoprazole. Diureties a. Site 1 Carbonic anhydrate inhibitors acetazolamide*, methazolamide. b. Site 2 High ceiling or loop diuretics is sulfamoyl anthranilic acids like furosemide*, azosemide and bumetanide in phenoxyaceticacidsethacrynicacid* c. Site3ThiazideandThiazidelikediuretics d. Site4Potassiumsparingdiureticssuchasspironoloactone triamtereneandamiloride LocalAnesthetics a. Aminoestersprocaine,tetracaine,benzocaine b. Aminoamideslidocaine*,mepivacaine,bupivacaine c. Aminoetherspramoxine d. AlcoholsBenzylalcohol,eugenol Hypoglycemics(Insulinnottobediscussed) st 1. Biguanidese.g.metforminb.Sulfonylureas1 Generationlike tolbutamide*,cholorpropamide,tolazamideand nd rd acetohexamide:2 Generationlikeglyburide,glypizide,3 Generationlikeglimeprideandrepaglimide. 2. Thiazolidinedionessuchastroglitazone,ciglitazone, rosiglitazoneandpioglitazone 3. Glycosidaseinhibitorslikeacarbose,vogliboseand miglitol. 58 |P a g e

3hrs/week
Hours 9

2.

3.

4.

5.

6.

Antiviral agents including HIV araA, indoxuridine*, amantadine*, acyclovir,ganciclovirandribavirin,HIVagentsbothnonnucleosides likenevirapine& delaviridineand nucleosideslike AZTandprotease inhibitors like indinavir, saqvunavir, ritonavir (only highlights of structure).Combinationtherapy Antineoplastics a. alkylating agents like mechlorethamine, chlorambucil*, cyclophosphamide*, mitomycin C, busulfan, carmustine, lomustine,dacarbazineandprocarbazine. b. Antimetabolites like azaserine, methotrexate*, 5fluorouracil, araC,6MPand6TG c. Antibioticslikedactinomycin,doxorubicin,bleomycin,andother natural products like vincristine, vinblastine, paclitaxel (only highlightsofstructuretobediscussed) d. Miscellaneous compounds like cisplatin and some newer derivatives e. Combinationtherapy *indicatessynthesistobediscussed

7.

ReferenceBooks: 1. 2. 3. 4. 5. 6. WilsonandGisvoldsTextbookofOrganicMedicinalandPharmaceuticalChemistry, th 11 Ed.,Eds.JohnHBlockandJohnMBeale,LippincottWilliams&Wilkins,2004. Foyes Principals of Medicinal Chemistry, Eds., T. L. Lemke and D. A. Williams, Williams&Wilkins,Baltimore,2002. th Medicinal Chemistry, Ashutosh Kar, 4 Edition, New Age International Publishers, 2007. The art of Drug Synthesis, Eds. Douglas S Johnson and Jie Jack Li, Wiley Interscience,2007. PharmaceuticalChemistry,Vol.1:DrugSynthesis,Eds.H.J.Roth,A.Kleemanand T.Beissewenger,EllisHorwoodLtd.,1988. TheOrganicChemistryofDrugSynthesis,DanielLednicer,Vols1to7,JohnWiley.

PHARMACEUTICALANALYSISIII
S.No. 1. Topic

3hrs/week
Hours 2

Basic concepts in spectroscopy Introduction: Electromagnetic radiation and interaction with matter, electronic spectra, wavelength, wavenumber, frequency, absorbance, transmittance, photometers, spectrophotometers. UVVis absorption spectroscopy electronic transitions and UV spectra, chromophores, Auxochromes, bathochromic and hypsochromic shifts, hyperchromism and hypochromism, Beer Lamberts law (Definition, derivation of mathematical expression, limitations), Applications of Beers law to single component analysis 59 |P a g e

2.

15

andmulticomponentanalysis(calibrationgraph,standardabsorptivity value, single standardisation, double standardisation, simultaneous equation method, difference spectroscopy, derivative spectroscopy), effect of solvents, Instrumentation Light sources, Filters, monochromators, cells, detectors, single beam and double beam spectrophotometers,withblockandraydiagrams. 3. Fluorescence spectroscopy origin of fluorescence and phosphorescence spectra, singlet and triple states, factors affecting fluorescence intensity, Quantitative fluorescence intensity, applications, Instrumentationlight sources, primary and secondary filters,monochromators,detectors. Infrared spectroscopy I.R. regions, requirements for I.R. absorption, vibrational and rotational transitions, dipole changes, types of molecular vibrations, potential energy diagrams (harmonic oscillator and anharmonic oscillator), Vibrational frequency, factors influencing vibrational frequencies, vibrational modes (normal mode, combination bands and overtone bands), Instrumentation: light source, frequency selector, sample preparation, detectors, double beam I.R. spectrophotometer (schematic diagram), Qualitative applications (identification of functional groups, identity by fingerprinting). ElectrochemicalmethodsTheory,Introduction,Instrumentationand Applicationsof: Coulometry Polarography&Pulsepolarography Amperometry Electrogravimetry 3

4.

5.

10

ReferenceBooks: 1. 2. 3. 4. 5. 6.
th SkoogPrincipals of Instrumental Analysis,4 edition, SaundersCollege Publishing, 1992,USA. th WillardH.H.L.L.Merrit&JohnA.DeanInstrumentalMethodofAnalysis,6 edition, 1986,CBSPublishers&Distributors,NewDelhi. rd Ewing Galen WInstrumental Method of Chemical Analysis, 3 edition, 1969, Mc GrawHillBookCompany,NewYork. th Beckett & StenlakePractical Pharmaceutical Chemistry, 4 edition, 1988, CBS Publishers&Distributors,India. th Vogel textbook of Practical Organic Chemistry 4 edition, 1984, Flbs & Longmans, London. James W. MunsonPharmaceutical Analysis: Modern methods, Marcel Dekker Inc., 1981,USA

60 |P a g e

PHARMACONOSYII
Drugsmentionedinboldarefordetailedstudy S.No. 1. Topic

3hrs/week

Hours 7

EvaluationandSignificanceofqualitycontrolofdrugsofnaturalorigin (DONO) Study oforganoleptic, microscopic,physical,chemicaland biological methodsofevaluation,Physicochemicalparameterssuchasmoisture content, ash value, acid insoluble ash, heavy metals content and pesticides residue, microbial bioburden etc. for crude drugs with respect to pharmacopeias. Introduction to W.H.O. guidelines and monographsofdrugsofnaturalorigin. Detailed morphology and microscopy of organized drugs and evaluationparametersforunorganizeddrugsmentionedinbold. Quantitative microscopy, Lycopodium spore method, Leaf constants, camera lucida, diagrams of microscopic objects to the scale with cameralucida. Detailed study of carbohydrates with respect to chemistry, sources preparationanduses.Allcellulosederivatives,starches,honey,inulin, alginic acid, gums (tragacanth, acacia, sterculia, Nanthan, guar). Mucilages(agar,isapghol,linseed)maltandmaltextract,dextram, pectins, chitin, hyaluronic acid, Study of sources, constituents and uses of carbohydrate containing herbs i.e. fig, bael, (Cassis latin fistula,tamarkindkernelpowder(TKP). Introductiontoorganicacids,fruits(citrus,tamarind,garcinia,amla). Introduction to Pesticides of natural origin Introduction study of followingwithrespecttotheiroccurrence,chemistryandapplications, Pyrethrum,nicotin,neem,Redsquill. Study ofchemistry, classification,extraction,properties, sources and usesofTannins. Detailed pharmacognostic study and application of galls, catechu (paleandblack),Kino,arjuna,ashoka,harda,behra Study of Lipids (Waxes, fats, fixed oils) their chemistry and classification.Studyoffollowingwithrespecttosources,extraction& composition of lipids and uses: Arachis, castor, sesame, linseed, jajoba, olive, almond, mustard, wheatgerm, cottonseed, coconut, safflower, sunflower, croton, neem, rice bran, hydnocarpus, cod halibutandsharkliveroil,Studyofkokumbutter,cocabutter,woolfat, spermacetiwax,beeswax,carnubawax,lecithin. Hydrocarbons Introduction to composition, properties & sources of alkanes, isoalkanes and anti isoalkanes, alkenes, ethylene, polyacetylenes (stearolic acid, falcarinone, dehydro matricaria esters, thiophenes fromtagetes). 61 |P a g e

2. 3.

5 3

4.

5. 6.

2 2

7.

8.

9.

10. Study of sources, structure and properties of sulphides from Allium species(A.cepa&Asativum) 11. Study of structure, occurrence and uses of essential amino acids, Study of peptides, proteins, protein hydrolysates, Sources, preparationandusesofgelatin. ReferenceBooks: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15.

2 2

TreaseD.&EvansW.C.:TextbookofPharmacognosyW.B.Saunders. TylerV.E.,BradyL.R.&RobbersJ.E.:PharmacognosyLeaFeibger,USA. WallisT.E.:TextbookofPharmacognosy:CBSPublishers,Delhi. KokateC.K.PurohitA.P.&GokhaleS.B.:Pharmacognosy:NiraliPublications,Pune. Harbone J.B.: Photochemical Methods: A guide to modern techniques of Plant Analysis:Chapman&Hall,London. BrunetonJ.:Pharmacognosy,Phytochemistry,MedicinalPlants,InterceptLimited. Vasudevan T.N. &Laddha K.S.: A TextbookofPharmacognosy, VrindaPublication House,Jalgaon. TheIndianPharmacopoeia:TheControllerofPublicationDelhi. BrainK.R.&TurnerT.D.:ThePracticalEvaluationofPhytopharmaceuticalsWright, Scientica,Bristol. lyengerM.A.&NayakS.G.:AnatomyofCrudeDrugs:ManipalPowerPress,Manipal. lyengerM.A.:PharmacognosyofPowderedDrugs:ManipalPowerPress,Manipal. KokateC.K.:PracticalPharmacognosy:VallabhPrakashan. Wagner,Bladt&Zgainski:PlantDrugAnalysis:SpringerVerlag. Khandelwal K.R.: Practical Pharmacognosy Technique and Experiments: Nirali Prakashan,Pune. Vasudevan T.N. Laddha K.S.: Practical Pharmacognosy: New Vrinda Publishing House.Jalgaon.

PHARMACEUTICSV
S.No. 1. Topic

3hrs/week
Hours 12

Tabletcoating Needfortabletcoating Sugar coating Raw materials, steps in detail, coating defects/problems Film coating Raw materials, Aqueous film coating, defects/problems Entericcoating CoatingEquipmentsConventional&modifiedpans,coating columns (fluidized bed coating), Spray equipment & other accessories. Q.C.ofcoatedtablets. Microencapsulation Definition,need/reasons,conceptsofcore&coat Methods with equipments Wuster process, coacervation 62 |P a g e

2.

phase separation, spray drying and related processes, interfacial polymerization,multiorifice centrifugalprocess, pan coating,solventevaporation. 3. Biologicalproducts Sutures & Ligatures definition, classification, catgut manufacturing&processingdetails,otherabsorbablesutures (brief), Non absorbable types silk, linen, polyamides, polyesters, polyolefins, metallic wires, Q.C. testing of sutures &ligatures. Blood products Need, problems/ hazards, Blood banking procedures. Whole human blood, Red cell cone, cone of platelets. Plasmapheresis, plasma, serum, Fractionation of plasma, StudyofsomefractionsclottingfactorslikeFibrinogen,AHF, Factor IX complex, Prothrombin, Albumin preparations, globulinpreparations.Q.C.aspectsofbloodproducts. Plasma substitutes (Plasma volume expanders) need, properties desired, exampleshydrolyzed gelatin based products,HETAstarch,Dextran(indetail) Glandularproducts Insulin extraction from pancreas, purification, insulin injections(official) ThyroidProcessing&product(official) ACTHpreparations Oxytocinandvasopressin ReferenceBooks: 1. 2. 3. 4. 5. 6. 7. LachmanLeon,LiebermanHerbertA.kanigJosephL.,TheTheoryandPracticeof rd IndustrialPharmacy,3 Edition1987,VarghesePublishingHouse,Mumbai. Deasy Patrick B., Microencapsulation and Related Drug Processes, Volume 20, 1984,MarcelDekkerInc.,NewYork. ColeGPharmaceuticalCoatingTechnology,Taylor&FrancisLtd.Bristol,PA,1995. Isaac GhebreSellassie, Pharmaceutical Pelletization Technology, Volume 37, 1989,MarcelDekkerInc.,NewYork. th E.A.Rawlins,Ed.,BentleysTextbookofPharmaceutics,8 Edn.,BallierweTindall, 1995. th S. J. Carter Ed., Tutorial Pharmacy, Cooper & Gunn, 6 Edn., CBS Publishers & distribution,India,1986. st Remington, The science and practice of pharmacy, 21 ed., Vol I & II, B. L., PublicationsPvt.Ltd.,2005. 4

3 4

63 |P a g e

COSMETICOLOGY
S.No. 1. Topic Definitionofcosmetics Historicalbackground Classificationofcosmeticsandfunctions

3hrs/week
Hours 1

2. 3.

Structure of skin, hair, nails and skin appendages and interaction with cosmetics. Toxicologyofcosmeticsirritationandsensitizationreactiontocosmetics, teststopredictsuchreactions Microbiologicalaspectsofcosmetics A brief review on perfumes, colors and other raw materials used in cosmetics. Study offollowing cosmetics with respect to raw materials,formulation, processingandqualitycontrol: Personnel hygiene products pedicure and manicure preparations, dental care preparations including tooth paste, tooth powder, mouth washes and denture cleaners, antiperspirantsanddeodorants. Facial makeup products skin creams and lotions including cleansing cream, cold cream, vanishing cream, foundation makeup, bleach cream, face powder, roage, lipstick, eye makeup,facepacks,andmoisturizers. Protective preparationshand and body creams and lotions, barrier preparations and emollient preparations, sunscreen and antisunburnpreparations,insectrepellants. Hair care preparations shampoo, womens and mens hair dressing, hair tonics and hair conditioners, hair rinses, hair colorants,hairwavingandstraighteningpreparations. Miscellaneous preparations Introduction to depilatories shaving preparations, nail products, bath oils, baby cosmetics, antilice preparations,herbalcosmetics. Schedule S of drug and cosmetics Act in relation to cosmetic manufacture,hygiene,pollutioncontrolecologicalconcern.

2 2

4. 5.

2 4

ReferenceBooks: 1.
th Harrys Cosmetology, 7 Ed., edited by J.B. Wilkinson & R.J. Moore, Longman Singapore,PublishersPvt.Ltd.

64 |P a g e

2. 3.

Cosmetics Science and Technology, Vol 1,2 & 3 M.S. Balsam & Edward Sagarin nd Ed.,2 edition,WileyIntersciencepublications,1972. rd CosmeticsFormulations, Manufacturing & Quality Control by P.P. Sharma 3 edition,VandanaPublicationsPvt.Ltd.,2005.

PHARMACEUTICALMANAGEMENT
1.

3hrs/week

2.

Basicprincipalsand types ofviableBusiness and enterprise. Healthcare industry positioning the pharmacist fit in the industry Major players, major brands, Core, auxiliaryandalliedproductsandmarketing,MajorComponentsofPharmabusiness (Lectures3) Financial management, Sourceoffunds, basicsof balancesheetandprofitanloss account. Product:DPCO,Costsofinputsinaproduct,Direct/indirect,Activitybasedcosting, Taxes,costbenefitanalysisofaproduct,SWOTanalysisofaproductincompetitive market,Nationalbudget. (Lectures3) Product Cost, value of a product, Market economics, COST OUTPUT relationship, PROFIT Management, Pricing analysis, Pricing policy Performance management MaterialsandInventorymanagementVendordevelopment,Stockaudits. (Lecturers3) Quality management: FDA regulations and approvals, WHO requirements, General awarenessofGlobalrequirementsofMHRA/MCA/TGA/USFDA/ISOupgradation. Sixsigmaconcept,ProductandprocessenvironmentManagement. (Lecturer3) Marketresearchprocess.Marketforecastingprocess.AnalysisofVolumeandgrowth ofPeripheralmarkets:Nutraceuticals. Cosmeceuticalsimplants. (Lecturer3) Marketing and sales: uniqueness of medical products marketing. Role of retail chemist,distributors,stockist,wholesaler,C&Fagents,whatisamarket,amarket share,competitivemarketing. Changeinoffingmalls,chainofcorporateretailsoutlets,BrandsandGenericmarket. (Lecturer6) Export market management: market search, preparations and Development, RegulatedandunregulatedmarketrequirementsContinentsandCountriesforGlobal market,Hospitals,Govt./Corporatepurchasers,ESISschemes,NGOs. (Lecturer3) Role of product development and clinical research, Major Diseases and major molecules, Clinical research, patent registration, IPR, PRODUCT and PROMOTIONALPOLICIES. (Lecture4) 65 |P a g e

3.

4.

5.

6.

7.

8.

9.

Other regulatory requirements Factory Act., Pollution Act., Firesafety and hazard managementHazaopstudy,(MSDS)safetydatasheets. (Lecturer3)

10. Leadership,Motivation,Delegation,communication,Conflictmanagement,Shopfloor management. (Lecture3) 11. Management concepts that help to create value Ps: product, place, price, people, packing,pace,OrganizationZSconcept. (Lecturer2) ReferenceBooks: 1) 2) 3) 4) 5) 6) 7) 8) 9) 10) 11) 12)
th MarketingManagement12 editionbyKotler,Loshy&Jha. nd MarketingManagement2 editionbyDr.RajanSaxena. IntroductiontoMarketingManagementbyAdrianPalmer. FinancialManagementbyPrasannaChandra. FinancialManagementbyI.M.Pandey. HumanResourcemanagementbyAshwathapa. Personnel&HumanResourceManagementbySubbaRao. Production&OperationsManagementbyK.Ashwathapa. Production&OperationsManagementbyS.N.Chary. Production&OperationsManagementbyS.A.Chunawala. BusinessLogistics/SupplyChainManagementbyRonaldBallon. IntroductiontoSupplyChainManagementbyRobertHanfiels.

PHARMACOLOGYIII
S.No. 1. Topic Autonomicnervoussystem BriefintroductiontoAnatomyandPhysiologyofANS Adrenergicagents(Sympathomimeticagents) adrenergicblockingagents Cholinergicdrugs(Cholinomimeticagentse.g.Anticholinesterases) Antochoilinergicdrugs a)Muscarrinicdrugs b)Nicotinicblockers Drugsactingatneuromuscularjunction(skeletalmusclerelaxants) Drugsactingonautonomicganglia(stimulantsandblockers) Cardiovascularsystem Drugsusedinthetreatmentof: Hypertension Congestivecardiacfailure Anginapectoris Cardiacarrhythmias 66 |P a g e

3hrs/week
Hours 18

2.

14

Drugsusedinthetreatmentofhyperlipoproteinaemia 3. Diureticdrugs 6

ReferenceBooks: 1. Goodman&GilmansPharmalogicalBasisofTherapeuticsJoelG.Hardman.Lee th E.Limbird,AlfredGoodmanGillMan11 Edition,TheMcGrawHillCompaniesInc, 2001. th Satoskar,R.S.BhandarkarSD&RegeN.N. Pharmacology&Therapeutics20 Edition,PopularPrakashan,2007. th Rang&DalePharmacology5 Edition,ChurchillLivingston2003. rd LippincottsIllustratedReviewPharmacologyLippincottRaven3 EditionHowland &NyeetsPublishersNY,2006. th LewisPharmacologyByCrossland5 Edition,ChurchillLivingstone. th LaurenceD.R.&BennetClinicalPharmacology9 Edition,Elsevier,NY,2006. rd Kulkarni, S. K. Handbook of Experimental Pharmacology 3 Edition Vallabh and Prakashan,NewDelhi,2005. th B. G. Katzung Basic and Clinical Pharmacology 9 Edition, Appleton and Lange publication,2004. rd Gosh M. N. Fundamentals of Experimental Pharmacology, 3 Edition, Hilton & Company,Calcutta,2005.

2. 3. 4. 5. 6. 7. 8. 9.

PHARMACOLOGYLABORATORYI
S.No. 1. Topic

4hrs/week
Hours

Experiments a) Dose Response Curve (DRC) of any agonist (eg. Ach/ Histamine) usingasuitableisolatedtissuepreparation. b) L V infusion a simulated model using rubber tube mounted on a wooden board (the students are taught to calculate the dose and concentration to be used, actual dilution for preparation of infusion, setting of L V, infusion including cannulation, setting of drop rate, for infusionoveragiven period of time) * Indian JournalofPharmacology, Vol39(I),Feb.2007:5759. (FreeFulltextavailableatwww.jiponline.com) Demonstration a)effectsofdrugonisolatedfrogheart(CDs/actual) Adrenaline,Acetylcholine AtropinePropranolol Effectofexcessofcalciumandpotassiumonisolatedheart EffectoflackofCa,Konisolatedheart Effectofdigitalisonhypodynamicheart b)Stimulatedexperiment(CDs)Expharm effectofdrugoneye effectofdrugonGImotility c)DemonstrationwiththehelpofCDsorKymographrecordings 67 |P a g e

2.

EffectNeostigmineonDRCofAch EffectofPanecuroniumonDRCofAch (Give the reading to thestudentsand ask themto plot thegraphs and draw the conclusion from the results, eg. Identify type of antagonism existing between two drug by studying the nature of thegraph,competitiveandnoncompetitive FindoutthepotencyofthedrugsbystudyingtheDRCbystudying IC50values) A CalculationofP 2valueforatropineusingAchasagonist. 3. Tutorials LaboratoryAnimalHandling CareandEthicsinAnimalexperimentation.

ReferenceBooks: 1. 2. 3. 4.
rd Kulkarni, S. K. Handbook of Experimental Pharmacology, 3 Edition Vallbh Prakashan,NewDelhi,2005. rd Gosh M. N. Fundamentals of Experimental Pharmacology, 3 Edition, Hilton & Company,Calcutta,2005. st S.B.Kasture.AHandbookofExperimentsinPreClinicalPharmacology,1 Edition, CareerPublications,2006. nd W.L.M.Perry,PharmacologicalExperimentsonIsolatedPreparations,2 Edition,E &SLivingstone,Edinburgh&London,1970.

Websites: IndianjournalofPharmaceuticaleducationandresearch,Vol.41(1),JanMar,2007 5261(www.ipper.org)

PHARMACOGNOSYLABORATORYI
S.No. 1. Topic

4hrs/week
Hours 3

Quantitativeestimationbylycopodiumsporemethod a) Determinationonnumberofparticles(starchgrains/stonecells/ lignifiedfibers)ingivensampleofcrudedrugs. b) Determinationof%purityofgingerpowder. c) Histochemical identification of starches (maize, rice, wheat, potato) IdentificationandMeasurementofdimensionsofdifferenttypesofstarch grainsandcalciumoxalatecrystals,trichomesandstomata. Leaf constants Stomatal index, stomatal number, palisade ratio, vein isletnumber,veinletterminationnumber. Determinationofalcoholsolubleandwatersolubleextractives,totalash value and acid insoluble ash and water soluble ash value for any one crudedrugasperI.P. Identification of unorganized drugs by chemical tests (agar, acacia, tragacanth,gelatin,sterculia,palecatechu,blackcatechuandKino) 68 |P a g e

2. 3. 4.

1 3 3

5.

6. 7.

Detectionofadulterantsfixedoils Visittomedicinalplantgarden Total

1 2 14

COSMETICOLOGYLABORATORY

4hrs/week

FormulationProcessing,PackagingandEvaluationofcosmeticpreparations. 1. Skincleansers Cleansingmilk Clearcleansinggel 2. Skinmoisturizers Coldcream Handandbodylotion Moisturizinglotion 3. Suncareproducts Medicateddustingpowder Sunscreencream 4. Facialcosmetics Vanishingcream Foundationlotion Eyeshadow Lipstick 5. Haircareproducts Clearliquidshampoo Antidandruffshampoo Menshairdressingpreparation 6. Nailcareproducts NailLacquer NailLacquerremover 7. Shavingpreparations Lathershavingcream Brushlessshavingcream Aftershavelotion 8. Dentalcareproducts Toothpaste Medicatedtoothpaste Mouthwash 9. Herbalcosmetics Powderfacescrub Antiwrinklecream Antiacnecream

69 |P a g e