Procedure: B. Preparation of the Standard Solutions (in 6-inch test tubes) Test Tube Blank 1 2 3 4 5 0.20 M KSCN, mL 1.

0 1.0 1.0 1.0 1.0 1.0 0.002 M FeCl3, mL 0 0.1 0.25 0.5 1.0 2.0 0.10 M HCl, mL 9.0 8.9 8.75 8.5 8.0 7.0

C. Preparation of Unknown solutions (in 6-inch test tubes) Test Tube Blank 1 2 3 Guide Questions: Experimental Detail: 1. What is the significance of using HCl in the solutions? 2. Why should KSCN be in excess in the standard solutions? 3. What is the use of AUTOZERO? 4. What is the use of blabk solution? 4. Why should the cuvette be rinsed three times with distilled water and then rinsed once with the solution to analyze? Results and Discussion: 1. Briefly discuss spectrophotometry: its principles, its instrumentation, and its application. 2. Explain why the absorbances in the standard solution corresponds only to the FeSCN2+ complex? 3. What does the y-intercept in the calibration curve mean? 4. Calculate the equilibrium constant from the Unknown solutions. Find for literature values of Keq and calculate for per cent deviation 0.002 M KCSN, mL 5.0 5.0 5.0 5.0 0.002 M FeCl3 0 3.0 4.0 5.0 0.10 M HCl, mL 5.0 2.0 1.0 0