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A C U T E S T I M U L A T O R Y EFFECTS OF P R O S T A G L A N D I N F 2 = ON S E R U M
P R O G E S T E R O N E C O N C E N T R A T I O N S IN ~ R E G N A N T A N D P S E U D O P R E G N A N T
PIGS ~
ABSTRACT:
The objective of this study was to investigate whether PGF2a, administered to
pregnant and pseudopregnant gilts in vivo, would cause an acute increase in serum
progesterone concentrations nrior to luteolysis. Pregnant (n = 9) and pseudopregnant
(n = 4) gilts were fitted with a jugular vein cannula on day 40, were treated with 3 ml
vehicle (control) i.m. on day 42 and with 15 mg PGF2ct on day 45. Blood samples were
collected at frequent (5 and 15 rain) intervals from 1 h before until 1 h after control and
PGF2a injections, at 15 rain intervals for 4 h, and then at 5, 6, 9, 21, 33, 45 and 57 h
post injection. Progesterone was measured by radioimmunoassay (RIA) in all samples.
Porcine LH was measured by RIA in samples collected frequently in the 1 h pre- and 1 h
post-injection periods. Serum progesterone concentrations were unchanged in both
pregnant and pseudopregnant animals in response to control injection on day 42.
However, in both pregnant and pseudopregnant gilts, PGF2ct injection on day 45
resulted in an acute increase (~75-80% above pre-treatment levels; p < 0.05) in serum
progesterone lasting ~1 h, followed by a return to pre-treatment levels by 2 h, and then a
decline to 1 ng/ml or less by 45-57 h (pregnant) or 21-57 h (pseudopregnant),
associated with luteolysis. Serum LH concentrations were unchanged between 1 h pre-
and post-treatment periods in response to either control or PGF2et-treatment, in both
pregnant and pseuodpregnant gi.'lts. These results indicate that PGF2winjection produces
a rapid and transient increase in serum progesterone concentrations which may result
from a rapid and direct stimulatory action of PGF2ct on porcine luteal cell progesterone
synthesis/secretion in vivo.
1 This material is based on work supportedby CSRS-USDA, under agreement No. 89-37240-4680 and
by the State of North Carolina.
Reprint requests to: Dr. John Gadsby, College of Veterinary Medicine, NCSU, 4700 Hillsborough,
Raleigh, NC 27606.
INTRODUCTION:
Animals;
For Experiments 1 and 3, pubertal gilts (Yorkshire-Lax&ace) were purchased
from a local supplier (N.C. Dept. of Agriculture, Cherry Unit, Goldsboro. NC) and
transported to NCSU Dept. of Animal Science Unit I swine facility, where they were
housed for the remainder of the study. For Experiment 2. pubertal gilts (Duroc) were
obtained from NCSU Unit II swine facility and transported to Unit I prior to the
beginning of the study. Estrus detection, breeding, hormone injections, cannulation and
blood sampling were performed at Unit I.
’ .
A. Preguant Gdt~.
B. Pseudoureenant Gilts:
Experiment 3: Four gilts were synchronized with oral ally1 trenbolone (for 14 days; see
Expt. 1) and the day of estrus following withdrawal of ally1 trenbolone was considered
day 0. On days 11-15, all gilts were treated with 5 mg (per gilt) estradiol valerate (Sigma
Chemical Co., St. Louis, MO; in corn oil) i.m. (once daily) to induce pseudopregnancy
(26). Animals were cannulated on day 40 (40.2 + 0.5 days; n = 4). All pseudopregnant
gilts were given the Control treatment (sterile water i.m.) on day 42 (42.2 + 0.5 days; n
= 4), followed by 15 mg PGF2o on day 45 (45.2 it 0.5 days; n = 4). Blood samples
were collected before and after treatments, following a protocol identical to that
described in Experiment 1. A second PGF2o injection (10 mg/gilt i.m.) was given
following the 9 h blood sample to ensure complete luteolysis.
Blood samoles:
Blood samples were allowed to clot at room temperature and serum was
collected after centrifugation at 2300 t-pm. Serum samples were stored at -20 C prior to
assay for progesterone and porcine LH.
Hormone radioimmunoa~
b) Luteinizing hormone: Serum samples collected during the immediate pre (-60 to 0)
and post (+5 to +60)-treatment (Control and PGF2o) periods were assayed for LH
concentrations using the double antibody RIA procedure described elsewhere (27). This
procedure employed the anti-porcine LH serum GDN #566 (kindly supplied by Dr.
G.D. Niswender, Colorado State Univ.) as the primary antibody, purified ovine LH
LER #1056-C2 (kindly supplied by Dr. L.E. Reichert, Jr., Albany Medical College) as
the radiolabelled (iodmated) ligand and unlabelled porcine LH LER #786-3 (supplied by
Dr. Reichert) as reference standards. Using pig serum LH pools of 5 ng/ml (high) and 1
ng/ml (low), interassay variation was 5.1% and 8.2% (5 assays). Irma-assay variation
was generally less than 5% for alI samples.
statistics:
Hormone data were analyzed using the least squares analysis of variance for
repeated measures using the General Linear Models procedures of the Statistical
Analysis System (28). In initial analyses, no significant differences (p > 0.05) were
observed between pregnant females in Experiments 1 and 2, suggesting that differences
in breed, use of estrus synchronization and the use of one versus two injections of
PGF2o were not confounding influences. Therefore, these data from Experiments 1 and
2 were pooled for subsequent analyses. Two pregnant females failed to abort after
PGF2o and were classified as a separate treatment in the statistical analyses. The
statistical model for the pregnant animals included animal, treatment (control, PGF2,
with abortion, or PGF2o without abortion), period (time before or after treatment),
animal-within-treatment and two-way interactions. The main effect of treatment was
tested using the animal-within-treatment mean square as the error term. When a
significant treatment-by-period interaction was present (p < O.OS), modifications of this
model were used to evaluate differences among periods within each treatment. When a
significant effect of period was observed, differences between the pretreatment (-1 h)
and all post-treatment (1 h to 57 h) periods were evaluated with pre-planned orthogonal
contrasts. Hormone data from pseudopregnant gilts were analyzed in a similar manner
as those from pregnant females except that in the statistical model, treatment groups
were control and PGF2o.
RESULTS:
hetmant frilts;
la
16
PGF2e - day 45
___(3___4___“““---’ I
6
4
I
0
-60 0 60 120 160 240 300 360 420 460 5 0
Minutes - pre and post injection
lb
20
I
18 ___e_. Control - day 42
16 _ PGF2a - day 45
14
12 I
.d
.’
10 .’
o-..,....-
-60 0 60 120 180 240 300 360 420 480 6 0
b) Serum LH: Figure 4a summarizes the mean serum LH concentrations for the
immediate pm (-60 to 0 min.; -1 h) and post (+5 to +60 min.; 1 h)-treatment periods. In
this Figure, data for all pregnant animals (n = 9) receiving PGF2, was combined since
all animals displayed an acute increase in serum progesterone concentrations lh post
treatment, whether or not abortion was induced. As shown, serum LH concentrations
did not differ significantly between pre and post-treatment periods for either control or
PGFb treatments.
. .
Pseudo-
b) Serum LH: As shown in Fig. 4b, serum LH concentrations did not differ
significantly between pre and post -treatment periods for either control or PGF2o
treatments.
DISCUSSION:
The data described in this paper show clearly that the administration of a
luteolytic dose of PGF2, to both pregnant and pseudopregnant gilts in vivo, causes an
acute increase in serum progesterone concentrations lasting approximately 1h.
Subsequently however, serum progesterone concentrations declined (significantly by 3-
4 h) to reach 1 ngIml or less between 21 and 57 h, a pattern more characteristic of a
luteolytic response to PGFzo-treatment (6-16). Similar transient elevations in serum
progesterone concentrations, preceding luteolysis, have been observed in response to
PGF2o administration to non-pregnant (7, 23. 24) and pregnant gilts (6) in vivo. In
view of the evidence indicating that PGF2o has dose-dependent stimulatoty effects on
progesterone production by porcine luteal cells during short term incubations (4-6 h) in
vitro (17; Earnest and Gadsby, unpublished), it is conceivable that the acute transient
increase in serum progesterone concentrations observed in this study results from a
direct stimulatory effect of PGFza on luteal steroidogenesis. Support for this conclusion
comes from the studies of Watson and Maule-Walker (29) who showed, using
superfused (in vitro) porcine luteal tissue slices, that a transient stimulation in
progesterone output preceded the inhibitory (“luteolytic”) effects of PGF2o on
progesterone production by this tissue.
In our studies we could find no evidence of an increase in serum LH
concentrations in association with the transient increase in serum progesterone
concentrations following PGF2o-treatment, suggesting that the stimulatory effects of
PGF2o on the corpora lutea of pregnancy and pseudopregnancy, were not mediated via
elevated pituitary LH secretion. This observation was of particular interest since the
corpora lutea of the extended luteal phases of pregnant and pseudopregnant pigs are
dependent on pituitary LH-secretion (25). Similarly, serum LH concentrations were
m found to be increased in association with increased serum progesterone
concentrations in response to PGF2o when given to non-pregnant cycling gilts (23,24).
Concerning a possible mechanism for the stimulatory effects of PGF2o on luteal
progesterone secretion, it has been shown that an early response of luteal cells to PGF2o
in vifro involves an immediate increase (within minutes) in intracellular calcium
concentrations (30-32). This increase in intracellular calcium levels in luteal cells
provoked by PGF2o is probably mediated by a stimulation of phospholipase C.
increased phospholipid turnover and elevated intracellular inositol triphosphate
concentrations (30, 33). as has been demonstrated for other hormones/growth factors
(34,35). In recent studies, it has been shown that short term (4h) incubations of porcine
(Ford, personal communication; Earnest and Gadsby, unpublished) and ovine (36)
luteal cells with the calcium ionophore A23187, also results in increased progesterone
production. Thus, it is possible that the acute stimulatory action of PGF2o on porcine
luteal cell steroidogenesis may be mediated by a rapid elevation in intracellular calcium
concentrations.
In the present study, the acute transient increase in serum progesterone
concentrations preceded complete luteolysis in a majority (7/9) of the pregnant animals
and all pseudopregnant animals, suggesting that there may be a causal relationship
between the early stimulatory, and later inhibitory (luteolytic), actions of PGF2o on
porcine luteal cells. Even in the two pregnant animals which did not abort, the acute
stimulatory phase was followed by a period of decreased luteal function reminiscent of
luteolysis, although normal luteal function recovered subsequently. In other studies (23,
24), acute transient increases in serum progesterone accompanied the action of PGF2o
given early in the estrous cycle, when it has been shown that PGF2o administration
results in incomplete luteolysis, followed by full luteal recovery (9), similar to that
shown in the two pregnant animals described above. Thus, although from our studies it
is not possible to determine a physiological role for the acute stimulatory actions of
PGF2o on the pig corpus luteum, it is tempting to speculate that the early effects of
PGF2o on luteal cells (i.e. increased phospholipid turnover and increased intracellular
calcium), of which the transient increase in steroidogenesis may be m
manifestation, may be important in initiating intracellular events which lead
ultimately to decreased progesterone production and diminished cellular function
associated with luteolysis (37).
4a
0 Control - day 42
0.6 -
0.6 -
0.4 -
0.2 -
0.0 +
-1 1
4b
0.4 -
0 COnWol -day 42
n WF2a -day 46
0.3 -
0.2 -
0.1 -
0.0 . 7
-1 1
ACKNOWLEDGEMENTS:
We thank the Department of Animal Science for supplying some of the pigs
used in this study, and for the use of the Unit I Swine Facility, where these studies were
conducted. In addition, we thank Dr. G.D. Niswender (Dept. of Physiology and
Biophysics, Colorado State University) for supplying the anti-porcine LH serum and
Dr. L.E. Reichert, Jr. (Dept. of Biochemistry, Albany Medical College) for supplying
porcine LH, used for the LH radioimmunoassay. We also thank Joan Metcalf for
assisting with estrus detection, animal care/feeding, Greg Richards, Jack Bradley and
Brian Ameson for their assistance with estrous detection, hormone injection and blood
sampling, and Mary Lancaster for performing serum progesterone assays, data analysis
and figure preparation. We gratefully acknowledge Dr. Billy Flowers for his invaluable
advice/assistance with statistical analysis of data, and we thank Drs. Jack Britt and Billy
Flowers for their helpful criticisms/comments during the preparation of this manuscript.
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