AS OCR Biology Revision Pack UNIT f211 Cells, exchange, transport Module 1 Cells Cell Structure

1. State the resolution and

magnification that can be achieved by a light microscope, a transmission electron microscope and a scanning electron microscope.

TEM

SEM

Maximum Resolution Maximum Magnification

2.

Light Microscope 0.2 micrometres X 1500

0.0001 micrometres Over x 1,000,000

0.005 micrometres Under x 1, 000, 000

Magnification How much bigger the image is than the specimen. Magnification = Length of Image / Length of specimen Resolution How well a microscope distinguishes between two points that are close together.
3. Explain the need for staining samples for use in light and electron microscopy

Explain the difference between magnification and resolution

In Light microscopes and TEMs the beam of lights/electrons pass through the object, and there is an image produced as some parts of the specimen absorb more light/electrons than others, but sometimes the specimen is transparent so it will look white because light/electrons pass through so the object is stained

Light Microscope Dye- usually methylene blue/eosin

4. Calculate the linear magnification of an image

Electron Microscope Specimen dipped in metal like lead, the metal ions scatter electrons to contrast.

Magnification = Length of Image / Length of specimen

5. Outline the functions of the structures.

Picture

Description

Function

Large and contains chromatin. Enclosed by a nuclear envelope double membrane. Nuclear pores go through the envelope. Nucleolus inside. Flattened membranous sacs called cisternae, rough is studded with ribosomes, smooth is not. Stack of flat, membrane bound stacks. [Pitta bread!]

Nucleus contains the cells genetic material. Chromatin contains DNA and proteins which regulate cell activities. Instructions for making proteins. RER transports proteins and SER is involved in lipid synthesis.

Golgi body receives proteins from ER and modifies them. Packages proteins into vesicles to transport them exocytosis Site of aerobic respiration, ATP is produced.

Sausage shaped. Double membrane separated by fluid filled space. Inner membrane is folded to form cristae and the middle part of the mitochondria is called the matrix.

In plant cells. Double membrane. Membranous sacs called thylakoids, plural=granum. Plural=grana. Spherical sacs surrounded by a single membrane, with no clear internal structure. Contains enzymes.

Site of photosynthesis, carbohydrate molecules made.

Enzymes break down cells. E.g. white blood cell lyosomes break down invading microorganisms and lyosome in the sperms head breaks down the material surrounding the egg. Site of protein synthesis, they are like an assembly line where mRNA from the nucleus is used to make proteins from amino acids. Eukaryotic- 80S Prokaryotic- 70S Involved in cell division to make spindles which move chromosomes in nuclear division.

TINY. Bound to ER to make RER and also in cytoplasm. Consist of two subunits.

Small tubes of microtubules. A pair can be found next to the nucleus in animal cells. Also in some protocytists. Membrane bound sac found in plants filled with cell sap.

Keep the plant supported, rigid and turgid. Also like a garbage disposal for plants.

Network of protein fibres

Support, movement. E.g. Chromosome movement in mitosis.

Thick layer, in plants. Made of cellulose in eukaryotic cells and murein in prokaryotic cells. Thin, flexible layer around all eukaryotic cells. Made of phospholipids and proteins.

Gives the cell strength and rigidity

It separates the cell contents from external environment and even controls movement of substances in and out of the membrane with receptor cells. In eukaryotic cells it contains organelles, in prokaryotic cells it contains enzymes needed for metabolic reactions. Genetic instructions

Enclosed jelly like substance within the cell membrane.

Circular and loose. Unprotected, unlike in eukaryotic cells. Plasmid Small circle of DNA

Exchange DNA easily and quickly between eukaryotic cells. Used in genetic engineering.

A thick polysaccharide layer outside of the cell wall

Useful for sticking cells together, and as a food reserve. Protects against phagocytosis and chemicals.

Rigid tail that rotates. The motor is embedded in the cell membrane and is driven by a H+ gradient across the membrane. Clockwise rotation drives the cell forwards, while anticlockwise rotation causes a chaotic spin. This is the only known example of a rotating motor in nature A tightly-folded area of the cell membrane

Propels the cell

Contains membrane bound proteins needed for respiration

6.

7.

Explain the importance of the cytoskeleton in providing mechanical strength to cells, aiding transport within cells and enabling cell movement.

Keep cells organelles in position with support Strengthen the cell to maintain its shape Transport material within the cell Help the cell to move, e.g. cilia and flagella by protein filaments.
Compare and contrast, with the aid of diagrams and electron micrographs, the structure of prokaryotic cells and eukaryotic cells.

Prokaryotic Cells

Eukaryotic Cells

Prokaryotic cells are smaller (0.2-2.0 m)

Eukaryotic cells are bigger 10-100 m

Dont have a nucleus, DNA floats free in cytoplasm and is circular

DNA is protected in nucleus and is linear

Less organelles and no membranous ones

Many organelles, plant & animal

70S Ribosomes

80S Ribosomes

8.

Compare and contrast, with the aid of diagrams and electron micrographs, the structure and ultrastructure of plant cells and animal cells.

Cell Membranes 9. Describe, with the aid of

diagrams, the fluid mosaic model of membrane structure

10. Describe the roles of the

components of the cell membrane; phospholipids, cholesterol, glycolipids, proteins and glycoproteins

Phospholipid molecules form a bilayer that is fluid as they always move, with hydrophilic heads and hydrophobic tails the protein molecules are scattered and can move. Some of these proteins have a carbohydrate chain attached to them, and these are called glycoproteins. Some lipids have a carbohydrate chain attached to them which are called glycolipids. Cholesterol is present in the membrane to provide mechanical stability.
11. Outline the effect of changing temperature on membrane structure and permeability

Cell Signalling How cells communicate with each other, One cell releases a messenger molecule (e.g. a hormone) The molecule travels to another cell (E.g. in blood) The messenger molecule is detected by the cell as it binds to a receptor on Its cell membrane
13. Explain the role of membrane-bound receptors as sites where hormones and drugs can bind.

12.

Explain the term cell signalling.

Membrane bound proteins can act as receptors for messenger molecules Receptor proteins have specific shapes so messenger molecule shapes are complementary on binding. Different cells have different receptor types and respond to different messenger molecules A cell that responds to a messenger molecule is a target cell Drugs either trigger a response in the cell or block the receptor to stop it working
14.

Diffusion the movement of particles from an area of higher concentration to an area of lower concentration Facilitated diffusion uses carrier and channel proteins Active transport Moves substances against a concentration gradient using ATP Endocytosis Cells take substances in, with part of a cells cell membrane surrounding it, the membrane pinches off to make a vesicle inside the cell containing the substance. Exocytosis Cells secrete substances. Vesicles with these substances pinch off from golgi body sacs and move towards the cell membrane. The vesicles fuse with the cell membrane and release their contents outside of the cell.
15. Explain what is meant by osmosis, in terms of water potential.

Explain what is meant by passive transport (diffusion and facilitated diffusion including the role of membrane proteins), active transport, endocytosis and exocytosis.

Osmosis is the diffusion of water molecules across a partially permeable membrane from a region of higher water potential to a region of lower water potential
16. Recognise and explain the effects that solutions of different water potentials can have upon plant and animal cells.

Cell Division, Diversity and Cellular Organisation

Explain the meaning of the term homologous pair of chromosomes

Humans have 46 chromosomes in total 23 pairs. One chromosome in each pair comes from the mother, and then the other comes from the father. Same size, same genes although they can have different versions of the genes (alleles).

Define the term stem cell

Stem cells are cells that are not specialized and can differentiate into specialized cells with mitosis and the correct stimulation.
Define the term differentiation, with reference to the production of erythrocytes (red blood cells) and neutrophils derived from stem cells in bone marrow, and the production of xylem vessels and phloem sieve tubes from cambium.

Bones are living organs containing nerves and blood vessels, and the main bones have marrow in the middle, adult stem cells divide and differentiate to replace worn out erythrocytes and neutrophils to fight infection. In plant cells stem cells are in the cambium. In the root and stem the stem cells of the vascular cambium divide to differentiate into the xylem and phloem, the vascular cambium then forms a ring inside the root and shoots. These cells divide and grow from the ring differentiating and moving away from the cambium.
Describe and explain, with the aid of diagrams and photographs, how cells of multicellular organisms are specialised for particular functions, with reference to erythrocytes (red blood cells), neutrophils, epithelial cells, sperm cells, palisade cells, root hair cells and guard cells.

Neutrophills protect the body against illness, they are flexible so they can engulf pathogens and they have lots of lysosomes with digestive enzymes that can break down the pathogens. Erythrocytes carry oxygen in the blood and they have a biconcave disc shape to give a large surface area to volume ratio for gaseous exchange, they dont have a nucleus so they have more room for haemoglobin. Epithelial cells cover organ surfaces and cilia can beat to move particles, and other like microvilli can fold in the cell membrane to increase surface area to volume ratio Sperm cells have a flagellum that enables them to swim to the egg and they have lots of mitochondria to provide energy to swim, the acrosome contains digestive enzymes so the sperm can penetrate the egg surface.
Explain the meaning of the terms tissue, organ and organ system.

A tissue is a group of similar cells that are specialized to work together to carry out a particular function. E.g. Ciliated epithelium, xylem tissue, squamous epithelium tissue, phloem tissue Organs are groups of different tissues that work together to form a function. E.g. Lungs squamous epithelium, ciliated epithelium, elastic connective tissue and vascular tissue.

Organ systems are different organs working together for a different function, e.g. the respiratory system is made of all of the organs, tissues and cells involved in breathing like the lungs, trachea, larynx, nose, the diaphragm and mouth.
Discuss the importance of cooperation between cells, tissues, organs and organ systems.

Mulitcellular organisms work efficiently as they have different cells that are specialized for various functions It is beneficial because every different cell can carry out a specialized function in a more efficient way than unspecialized cells could. Each cell depends on the other cells for the functions it cannot carry out So cells, tissues and organs in multicellular organisms cooperate to keep the organism alive and working well. E.g. Muscle cells can move well but to do so they need oxygen, so they need erythrocytes to carry oxygen to them from lungs. Module 2 Exchange and Transport Exchange Surfaces & Breathing
Explain, in terms of surface area:volume ratio, why multicellular organisms need specialised exchange surfaces and single-celled organisms do not.

Smaller organisms have a higher surface area to volume ratio, so single celled organisms can diffuse substances directly in or out of the cell across the cell surface membrane. However, diffusion in multicellular organisms is too slow because: Some cells are deep in the body, so large diffusion distance from external environment Large animals have a smaller surface area to volume ratio so it is difficult to exchange enough substances to supply a large volume of animal through a small outer surface A lot of multicellular organisms are active so many cells are respiring and so they need a constant rapid supply glucose and oxygen.
Describe the features of an efficient exchange surface, with reference to diffusion of oxygen and carbon dioxide across an alveolus.

a large surface area a thin permeable surface a moist exchange surface

Describe the features of the mammalian lung that adapt it to efficient gaseous exchange.

On inhalation the air enters the trachea The trachea divides into two bronchi, and one bronchus goes to each lung The bronchus divides into bronchioles, which end in small air sacs called alveoli where gaseous exchange occurs. The ribcage, intercostals muscles and diaphragm work together to move air in/out
Describe the distribution of cartilage, ciliated epithelium, goblet cells, smooth muscle and elastic fibres in the trachea, bronchi, bronchioles and alveoli of the mammalian gaseous exchange system

Goblet cells secrete mucus which traps pathogens and dust in the inhaled air, the cilia on the surface of cells beat rhythmically to waft mucus at the back of the throat where its swallowed and the stomachs acidity kills any pathogens. Elastic fibres in the walls of the trachea, bronchi, bronchioles and alveoli aid ventilation. They stretch and recoil to push air out when exhaling. Smooth muscle is in the walls of the trachea, bronchi and bronchioles and it can relax to dilate the lumen, to allow air in/out easily. Rings of cartilage are in the trachea walls and bronchi to give support and flexibility to stop them collapsing on inhalation and pressure dropping.

Part of lung

Cartillage

Smooth Muscle

Elastic Fibres

Goblet Cells

Epithelium

Trachea

Large-C shape

Yes

Yes

Yes

Ciliated

Bronchi

Small pieces Yes

Yes

Yes

Ciliated

Larger Bronchiole

None

Yes

Yes

Yes

Ciliated

Small Bronchiole

None

Yes

Yes

No

Ciliated

Smallest Bronchiole

None

No

Yes

No

No cilia

Alveoli

None

No

Yes

No

No cilia

Outline the mechanism of breathing (inspiration and expiration) in mammals, with reference to the function of the rib cage, intercostal muscles and diaphragm

Inspiration Intercostal and diaphragm muscles contract Ribcage moves upwards and outwards and diaphragm flatten increasing volume of thorax As the volume of the thorax increases lung pressure decreases below atmospheric pressure Air flow into the lungs Active process needing energy (ATP)

Expiration Intercostal and diaphragm muscles relax Ribcage moves downwards inwards and diaphragm curved again The thorax volume decreases causing air pressure to increase above atmospheric pressure Air forced out of lungs Passive process not requiring energy.

Explain the meanings of the terms tidal volume and vital capacity.

Tidal Volume Volume of air inhaled/exhaled in a normal breath- normally 0.4 dm3 Vital Capacity The maximum volume of air that can be inhaled/exhaled

Describe how a spirometer can be used to measure vital capacity, tidal volume, breathing rate and oxygen uptake

1. 2. 3. 4.

A spirometer has an oxygen filled chamber with a lid that can move The person will breathe through a tube connected to O 2 chamber On inspiration/expiration the lid of the chamber moves up/down The movements are recorded by a pen attached to the lid of the chamber, and writes on a rotating drum to create a spirometer trace 5. The soda lime in the tube the person breathes into absorbs CO2
Analyse and interpret data from a spirometer.

Transport in animals
Explain the meaning of the terms single circulatory system and double circulatory system, with reference to the circulatory systems of fish and mammals.

In a single circulatory system blood passes through the heart once, whereas in a double circulatory system the blood goes through the heart twice for each complete circuit of the body.
Explain the meaning of the terms open circulatory system and closed circulatory system, with reference to the circulatory systems of insects and fish.

Mammals and fish have closed circulatory system, which means the blood is inside blood vessels. The heart pumps blood into arteries which branch into capillaries, and substances like oxygen and glucose diffuse from blood in capillaries to body cells but blood will stay in the blood vessels, veins take blood back to the heart. Whereas insects have open circulatory systems meaning that blood isnt contained in blood vessels, it flows free through the body cavity.

Describe, with the aid of diagrams and photographs, the external and internal structure of the mammalian heart.

Describe the cardiac cycle, with reference to the action of the valves in the heart

SAN is a pacemaker and sends out a wave of excitation that spreads over atrial walls Ventricles are electrically insulated by a collagen tissue band, the right and left atria contract The wave of excitation spreads to the AVN from the SAN After a small delay of 0.1s so the atria have emptied the AVN passes the wave to the bundle of His The Bundle of His passes the wave to the Purkyne fibres The Purkyne fibres carry the wave of excitation to the apex of the ventricle walls causing them to contract simultaneously from the bottom up.

Describe the cardiac cycle, with reference to the action of the valves in the heart

Interpret and explain electrocardiogram (ECG) traces, with reference to normal and abnormal heart activity.

Too fast heartbeats (i.e. 120 beats a minute) are fine during exercise, at rest however it shows that the heart doesnt pump blood efficiently. With the atria contracting but the ventricles not, e.g. some Ps not followed by a QRS this could indicate a problem with the AVN, i.e. no impulse from the atria to ventricles. Fibrillation is when the atria lose their rhythm and dont contract properly, resulting in chest pain, fainting or even death.
Describe, with the aid of diagrams and photographs, the structures and functions of arteries, veins and capillaries.

Explain the differences between blood, tissue fluid and lymph.

Blood Erythrocytes
Yes

Tissue Fluid
No

Lymph
No

Explanation
Erythrocytes are too large and cannot get through capillary walls to tissue fluid Most white blood cells are in lymphatic system & only go to tissue

Leukocytes

Yes

Few

Yes

Platelets Proteins Water

Yes Yes Yes

No Few Yes

No Antibodies Yes

Dissolved Solutes

Yes

Yes

Yes

fluid on infection In tissue fluid if capillaries are damaged Too big to go through capillary walls Tissue Fluid and Lymph have a higher water potential than blood Solutes like salt can move freely

Describe how tissue fluid is formed from plasma.

Tissue fluid surrounds the cells and is made from substances that leave the blood, like oxygen, water, etc. At the start of the capillary bed the pressure inside the capillaries near the arteries is more than the pressure in tissue fluid, this difference forces fluid out of the capillaries and into spaces surrounding cells to form tissue fluid. When the fluid leaves the pressure is less in the capillaries, so the pressure is lower at the end of the capillary bed nearest veins. Because of the fluid loss the water potential at the end of the capillaries nearer the veins is lower than the water potential in the tissue fluid, so some water will re enter the capillaries from the tissue fluid near the veins by osmosis down a water potential gradient.

Describe the role of haemoglobin in carrying oxygen and carbon dioxide.

Hb + 4O2 HbO8 Erythrocytes contain Hb, a large protein with a quaternary structure (Because it has 4 polypeptide chains) CONFIRMATIONAL CHANGE Each chain has a haem group containing iron, and is why Hb is red. Hb has a high affinity for oxygen and each molecule can carry 4 O 2 molecules It is reversible, and oxygen can dissociate from Hb near the body cells to leave Hb. The pO2 is a measure of O2 concentration, the greater the concentration the higher the partial pressure. So pCO2 is the measure of CO2 concentration in a cell. Haemoglobins affinity for oxygen varies depending on the partial pressure of oxygen, i.e. Oxygen loads onto haemoglobin to form oxyhaemoglobin when there is a high partial pressure, and unloads at lower partial pressures. Oxygen enters blood capillaries at the alveoli, alveoli have a high partial pressure so oxygen will combine with Hb to form oxyhaemoglobin. Respiring cells use oxygen and have a lower partial pressure so erythrocytes take oxyhaemoglobin to respiring tissues and the oxygen dissociates.

Then the more oxygen A lot of CO2

haemoglobin goes back to the lungs to pick up diffuses into erythrocytes to form carbonic acid by the enzyme carbonic anhydrase. 10% combines with haemoglobin and is carried to the lungs. The carbonic acid splits to produce H+ ions and Hydrogencarbonate ions. The increase in H+ ions causes oxyhaemoglobin to unload oxygen so it can take up H+ ions to stop the cell acidity increasing (BUFFER) to form haemoglobinic acid. The Hydrogencarbonate ions diffuse out of the erythrocytes and transported in blood plasma. When the reaches lungs to low pCO2 causes Hydrogencarbonate and H+ ions to which then diffuses into the alveoli and is

are blood recombine into CO2 exhaled.

Explain the significance of the different affinities of fetal haemoglobin and adult haemoglobin for oxygen.

The fetus gets oxygen from its mothers blood across the placenta By the time the mothers blood reaches the placenta its oxygen saturation has decreased as some has been used by the mothers body. For the fetus to get enough oxygen to survive its haemogobin must have a higher affinity for oxygen than adults. Transport in plants
Explain the need for transport systems in multicellular plants in terms of size and surface area to volume ratio.

Plants need water, CO2 minerals like nitrates and potassium, and sugars to live and they need to get rid of waste substances. They are multicellular and have a small surface area to volume ratio so need transport systems to move substances to and from cells quickly as diffusion alone is too slow.

Describe, tissue in roots,

with the aid of diagrams and photographs, the distribution of xylem and phloem stems and leaves of dicotyledonous plants.

Leaf Cross

Section

Root Cross Section

Describe, with the aid of diagrams and photographs, the structure and function of xylem vessels, sieve tube elements and companion cells.

Phloem tissue transports solutes like sucrose around plants, it is only a transport tissue. Sieve tube elements are living cells that form the tube for transportation of solutes around the plant, they are joined end-end to make sieve tubes. The sieves are end walls with holes in them for solutes to pass through, although they have no nucleus, a

thin layer of cytoplasm and few organelles. The cytoplasm of nearby cells is joined through holes in sieve plates. Companion cells are there for each sieve tube element to carry out metabolic processes for the sieve tube elements that cannot survive on their own as they have no nucleus, etc., and itself- e.g. they provide energy for active transport of solutes .

Define the term transpiration.

The loss of water from the plants surface

Explain why transpiration is a consequence of gaseous exchange.

A plant must open its stomata for absorption of carbon dioxide for photosynthesis, which as a consequence allows water to escape because there is a higher water potential inside the leaf than outside. So water moves out of the leaf by osmosis down the water potential gradient.
Describe, with the aid of diagrams, how a potometer is used to estimate transpiration rates.

1. Cut a shoot under water to stop air from going into the xylem at a slant to increase surface area to volume ratio for water uptake 2. Check that the apparatus has no air bubbles and is full with water 3. Put the shoot into the apparatus underwater to prevent air entering 4. Remove the photometer from the water and make it air and water tight 5. Dry the leaves, let the shoot acclimatize and shut the tap 6. Keep conditions constant throughout the experiment 7. Record the starting position of the air bubble 8. Start a stopwatch and record the distance moved by the bubble per unit time
Explain, in terms of water potential, the movement of water between plant cells, and between plant cells and their environment.

Light Temperature

Humidity Wind

Lighter= faster rate of transpiration as the stomata open for photosynthesis Higher= faster rate as water molecules have higher kinetic energy so they evaporate from cells quicker, increasing the water potential gradient between inside and outside of leaf making water diffuse out quicker. Lower= faster, if the air around the plant is dry the water potential gradient between the leaf and air is steeper Higher= faster, air movement blows the water molecules from the stomata, steepening the water potential gradient

Describe, with the aid of diagrams and photographs, how the leaves of some xerophytes are adapted to reduce water loss by transpiration.

Describe, with the aid of diagrams, the pathway by which water is transported from the root cortex to the air surrounding the leaves, with reference to the Casparian strip, apoplast pathway, symplast pathway, xylem and the stomata.

Water travels through the roots via the root cortex into the xylem by two ways

The Symplast Pathway Goes through living parts of the cells, the cytoplasm. The cytoplasm of nearby cells connect through plasmodestmata, which are small spaces in cell walls.

The Apoplast Pathway Goes through non living parts of the cells, the cell walls, the walls are absorbent and water can diffuse by osmosis through them and pass through spaces between them.

When water is in the Apoplast pathway it goes to the endodermis cells in the root, but the path is blocked by the Casparian strip- which is just a waxy strip. The water then must take the Symplast pathway. This is not a hindrance because the water than has to go through the cell membrane which controls substances entering/leaving. If the water goes past the barrier it moves into the Xylem. The main pathway used is the Apoplast pathway as it provides the least resistance.
Explain the mechanism by which water is transported from the root cortex to the air surrounding the leaves, with reference to adhesion, cohesion and the transpiration stream.

Cohesion and tension move water up from roots to the leaves against gravity, water evaporates from the leaves at the top of the xylem via transpiration This creates suction/tension which pulls more water into the leaf Water molecules are cohesive, meaning they stick together, so if one is pulled into the leaf so are more. The whole column of water in the xylem moves upwards, and it enters the stem through the roots. Adhesion is the water molecules being attracted to the walls of the xylem vessels, helping water rise up.
Explain translocation as an energy-requiring process transporting assimilates, especially sucrose, between sources (e.g. leaves) and sinks (e.g. roots, meristem).

Translocation is the movement of dissolved substances like sucrose amino acids when they are needed in a plant- called assimilates. This requires energy and happens in the phloem. Translocation moves substances from sources (where it is produced- higher concentration) to sinks (where it is used- lower concentration) E.g. The source for sucrose is the leaves and the sinks are mainly food storage organs and the meristems (growth areas) in the roots, stems and leaves. Enzymes maintain the concentration from the source to the sink by changing the dissolved substances at the sink, like by breaking them down or changing them into something else, to make sure there is a lower concentration at the sink than the source to keep a steep concentration gradient. and
Describe, with the aid of diagrams, the mechanism of transport in phloem involving active loading at the source and removal at the sink, and the evidence for and against this mechanism.

At the source active transport is said to actively load the dissolved solutes into sieve tubes of the phloem. Lowering the water potential inside sieve tubes and water enters them via osmosis. Creating a high pressure inside the sieve tubes at the source end of the phloem. At the sink the solutes are removed from the phloem to be used Increasing water potential inside the sieve tubes so water leaves by osmosis Lowering pressure inside the sieve tubes Creating a pressure gradient from the source to the sink This gradient is responsible for pushing solutes along the sieve tubes to where they are required in the plant.

For Removing a ring of bark from a tree taking the phloem not the xylem from a woody stem a bulge will form above the ring. On analysis of the fluid in the bulge, there will be a higher sugar concentration above the ring than below- so there must be a downward sugar flow. Aphids pierce the phloem with their mouthparts and sap flows into them, the sap flows out quicker nearer the leaves than further down the stem, so there must be a pressure gradient. A metabolic inhibitor stopping ATP production in the phloem stops translocation, proving it is active transport. There are experimental mass flow models

Against Sugar travels to many sinks not one with the highest water potential, as the model indicates

Sieve plates would make a barrier to mass flow, a lot of pressure would be needed for solutes to pass at a reasonably quick rate