Tryptone Soy Agar

DM 225 A general purpose isolation medium not based on meat products. Typical formula* Casein hydrolysate, enzymic Agar A Soy peptone Sodium chloride pH approx.7.3 grams per litre 15.0 12.0 5.0 5.0 satisfactory. Combining the two peptones gives a medium supporting the growth of a wide variety of aerobic and anaerobic micro-organisms, with or without added blood, giving typical colonies and clear haemolytic reactions. "Blood Agar" Cool to about 45-50C and add asceptically 510% sterile defibrinated animal blood. Mix gently and pour. "Chocolate Agar" Add 5-10% sterile defibrinated animal blood to the medium at 75C, mix gently and maintain at this temperature for 10 minutes. Pour plates. Alternatively heated blood plates may be prepared by keeping blood plates at 55C for 1-2 hours.

In Use

Directions
1. Suspend 37g of powder in 1 litre or the contents of the sachet, with agitation, in the stated volume of distilled or deionised water. 2. Autoclave at 121C (15p.s.i.) for 15 minutes. 3. Thoroughly mix before pouring.

Description
Leifson produced several peptones from casein, and found that pancreatic digests gave a peptone equivalent to other peptones prepared from pure 2 proteins. Brewer found from studies of various vegetable extracts that soy peptone was the most
1

References
1. Leifson E. Bull Johns Hopkins Hosp. 1943; 72: 179-199 2. Brewer JH. J Bacteriol. 1943; 46: 395-397

Tryptone Soy Broth

DM 226 Soybean Casein Digest Medium USP, a medium for sterility tests. Typical formula* Casein hydrolysate, enzymic di-Potassium hydrogen phosphate Soy peptone Sodium chloride D-Glucose pH approx. 7.3 grams per litre 17.0 2.5 3.0 5.0 2.5 Tryptone Soy Broth utilises the pancreatic digest of casein and the soy peptone which are used in Tryptone Soy Agar. Accordingly, the medium will grow many micro-organisms without the addition of blood or serum. The medium is listed by the U.S. Pharmacopoeia as a medium for detecting low level contamination by aerobic bacteria and fungi. The addition of a small amount of agar permits growth of anaerobic strains deep in the broth. The presence of glucose in the medium, while permitting excellent growth, prohibits its use as a maintenance medium for acid sensitive and fastidious organisms.
1

Directions
1. Dissolve 30g of powder in l litre or the contents of the sachet in the stated volume of distilled or deionised water. 2. Distribute into suitable containers. 3. Autoclave at 121 C (15p.s.i.) for 15 minutes.

References
1. U.S. Pharmacopoeia 1970; XVIII: 852.

MAST is a Registered Trademark *Formulation may change to meet performance criteria

Description

PG 4/97 V0.1