Casework Questions To Ask Your Representatives. 1. 2. 3. 4. Specific questions about vaccines, vaccine safety or the vaccine-autism link.

Why has this issue been largely ignored. When will we see a vaccinated verses an unvaccinated study. Why has the U.S. Vaccine Court made it difficult to prove obvious vaccine injury 5. Why wasnt Dr. Boyle of the CDC not prepared and did not have a clue about the studies she was asked about the 2012 Autism Hearing? 6. Why is Poul Thorsen still at large after lying, embezzling and fleeing the United States while his studies continues to be cited as the gospel truth? 7. Why is it ethical for vaccine-patent holder Dr. Paul Offit to be the nations goto guy when vaccines, autism and the vaccine-autism link hit pharmaceutically-funded mainstream news sites? This list of questions was compiled by Cathy Jameson in an a recent article. Jameson, C. (2013). An autism mom show how easy it is to be a congressional armchair advocate. Age of Autism. Retrieved from http://www.ageofautism.com/2013/06/an-autism-mom-shows-how-easy-it-is-tobe-a-congressional-armchair-advocate.html#comment-captcha Here are some specific Questions I have regarding the science. 1. Why is it that when conducting vaccine safety data and efficiency trials they only measure humoral immunity? In essence in Human trials they send the patients home with diary cards to fill out and document anecdotal evidence and then draw blood for antibody response. Seems like a simplistic view based on cell-mediated immunity. I want to know the measured response of macrophages, natural killer cells, cytotoxic T-lymphocytes because we know in an over abundance will cause a cascade of inflammation leading to grades of encephalopathy. In the U.S. a known table injury for vaccine adverse events is encephalopathy (HRSA, n.d.). Yet there is no effort in knowing the chain reaction at the molecular level at least by our health authority. HRSA. (n.d). Vaccine injury table. U.S. Department of Health and Human Services. Retrieved from http://www.hrsa.gov/vaccinecompensation/vaccinetable.html 2. How does the body distinguish between a vaccine antigen and a naturally occurring antigen? Recognition of antigens in CNS parenchyma results in retention of recruitment of T-lymphocytes, leading to inflammation. Blymphocytes probably also randomly traffic through the brain in a similar fashion and, if they find specific antigens, they aggregate and produce IgG antibodies (Block et al, 2007).

Block ML, Zecca, Hong J-S. Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. Nat Rev Neuroscience 2007; 8:57-69. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed?term=%22Nature+reviews.+Neuroscience %22[Jour]+AND+57[page]+AND+2007[pdat] 3. If antibodies trigger inflammation why are they not studying this in vaccine safety trials? 4. Can the ingredients in vaccines trigger cell-mediated immunity? Microglia cells have receptors that enable them to sense damaged tissue and to recognize viruses, environmental and endogenous toxins, and other pathogens. Such recognition leads to upregulation (activation) of microglia cells (Block et al, 2007). This is what I find most disturbing. However, persistent activation of microglia has damaging effects and is thought to contribute to the neurodegeneration that occurs in Alzheimers disease, Parkinsons disease, HIV encephalopathy, and other conditions. Microglial activation also develops progressively with advancing age in absence of stimulation (Block et al 2007) And this is specific with antigens. The most important of these cells are the perivascular monocytes, which reside just outside the vascular basement membrane. These cells are the main antigenpresenting cells of the CNS, thus playing an important role in immune reactions involving the brain (Block et al 2007). So I ask you why isnt this part of the safety analysis when studying vaccines? Why are they just looking at one arm of immunity never bothering to see what effects there are on the other? Especially because the risks of over-activation is so damaging Block ML, Zecca, Hong J-S. Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. Nat Rev Neuroscience 2007; 8:57-69. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed?term=%22Nature+reviews.+Neuroscience %22[Jour]+AND+57[page]+AND+2007[pdat]

5. Innate immunity comes first, neutrophils, basophils, eosinophils, macrophages (can act as a bridge to the adaptive immune response), dendritic cells (can act as a bridge to the adaptive immune response). Adaptive Immunity (occurs second), Humoral immunity, Th2 CD4+T cells, B Cells Cell Mediated Immunity, Th1 CD4+ T cells, CD8+T cells (also called Cytotoxic T cells). And there are other cell types (e.g. Natural Killer Cells) and other response profiles (e.g. Th17). Why isnt this entire process studied during vaccine safety trials?

6. I would like to ask specifics on cell-production thru specific cell activations. (A) Antigen/Pathogen/Toxin presents itself in the CNS. (B) The cell-mediated (TCell) immune system is activated. (C) Cytotoxic cells, (IFN)-y, (TNF)-a, (IL)-6, IL-10 and macrophages are produced when T cells (cell mediated immunity) is activated. This is how its described in MedScape: These cytokines are produced by activated T cells and histiocytes that infiltrate all tissue and lead to tissue necrosis and organ failure (Ravelli, 2012). Ravelli, A. (2012). Macrophage Activation Syndrome. Medscape. Retrieved from http://emedicine.medscape.com/article/1380671-overview#a0104 7. So again why isnt this area of the immune system included in safety analysis? When you assault the brain repeatedly in utero and after delivery with neurotoxins, antigens and pathogens hoping to get good antibody uptake rates to combat against infectious disease? What is that doing to the cell-mediated system that produces or activates cytokines that are known to cause neuro deterioration, encephalopathy, AZ to name a few disorders? 8. Can we discover the mechanism and design protocols to protect those with increased risks? Here is a CDC report that outlines the systemic reactions in greater detail: CDC. (1996). Update: vaccine side effects, adverse reations, contraindications, and precautions recommendations of the advisory committee on immunization practices (ACIP). MMWR. Retrieved from http://www.cdc.gov/mmwr/preview/mmwrhtml/00046738.htm 9. Why isnt the HHS doing a better job offering General Pediatricians training on how to better pre-screen their patients who have mitochondrial disease (MD) which is known to increase the risk of vaccine adverse events that result in an autism diagnosis? According to the government expert witness of Hannah Poling case, Dr. Andrew Zimmerman, M.D. Pediatric Neurologist, The cause for regressive encephalopathy in Hannah at age 19 months was underlying mitochondrial dysfunction, exacerbated by vaccine-induced fever and immune stimulation that exceeded metabolic energy reserves. This acute expenditure of metabolic reserves led to the permanent irreversible brain injury. Thus, if not for this event, Hannah might have led a normal full productive life. Presently, I predict Hannah will have a normal lifespan but with significant lifelong disability (Zimmerman, 2007).

Zimmerman, A. (2007). Hannah Poling (DOB: 12/27/98); Report of the Office of Special Masters, United States Court of Federal Claims, November 9, 2007. Kennedy Krieger Institute. Retrieved from http://www.scribd.com/doc/115393727/Andrew-Zimmerman-Poling-v-HHSExhibit-3 There is an associated prevalence of MD and autistics. Frye et al. (2013). Unique acyl-carnitine profiles are potential biomarkers for acquired mitochondrial disease in autism spectrum disorder. Translational Psychiatry. Retrieved from http://www.nature.com/tp/journal/v3/n1/full/tp2012143a.html Excerpt: Autism spectrum disorder (ASD) has been associated with mitochondrial disease (MD). Interestingly, most individuals with ASD and MD do not have a specific genetic mutation to explain the MD, raising the possibility of that MD may be acquired, at least in a subgroup of children with ASD