1.2.1 Acacia arabica: (Babhul) It is found all over India mainly in the wild habitat.

The plant extract acts as an antidiabetic agent by acting as secretagouge to release insulin. It induces hypoglycemia in control rats but not in lloxanized animals. Powdered seeds of Acacia arabica when administered (2,3 and 4 g/kg body weight) to normal rabbits induced hypoglycemic effect by initiating release of insulin from pancreatic beta cells. 1.2.2 Aegle marmelos: (Bengal Quince, Bel or Bilva) Administration of aqueous extract of leaves improves digestion and reduces blood sugar and urea, serum cholesterol in alloxanized rats as compared to control. Along with exhibiting hypoglycemic activity, this extract also prevented peak rise in blood sugar at 1h in oral glucose tolerance test . 1.2.3 Allium cepa: (onion) Various ether soluble fractions as well as insoluble fractions of dried onion powder show antihyperglycemic activity in diabetic rabbits. Allium cepa is also known to have antioxidant and hypolipidaemic activity. Administration of a sulfur containing amino acid from Allium cepa, Smethyl cysteine sulphoxide (SMCS) (200 mg/kg for 45 days) to alloxan induced diabetic rats significantly controlled blood glucose as well as lipids in serum and tissues and normalized the activities of liver hexokinase, glucose 6-phosphatase and HMG Co A reductase [18, 19]. When diabetic patients were given single oral dose of 50 g of onion juice, it significantly controlled postprandial glucose levels . 1.2.4 Allium sativum: (garlic) This is a perennial herb cultivated throughout India. Allicin, a sulfur-containing compound is responsible for its pungent odour and it has been shown to have significant hypoglycaemic activity. This effect is thought to be due to increased hepatic metabolism, increased insulin release from pancreatic beta cells and/or insulin sparing effect. Aqueous homogenate of garlic (10 ml/kg/day) administered orally to sucrose fed rabbits (10 g/kg/day in water for two months) significantly increased hepatic glycogen and free amino acid content, decreased fasting blood glucose, and triglyceride levels in serum in comparison to sucrose controls. S-allyl cystein sulfoxide (SACS), the

precursor of allicin and garlic oil, is a sulfur containing amino acid, which controlled lipid peroxidation better than glibenclamide and insulin. It also improved diabetic conditions. SACS also stimulated in vitro insulin secretion from beta cells isolated from normal rats. Apart from this, Allium sativum exhibits antimicrobial, anticancer and cardio-protective activities. 1.2.5 Aloe vera and Aloe barbadensis Aloe, a popular houseplant, has a long history as a multipurpose folk remedy. The plant can be separated into two basic products: gel and latex. Aloe vera gel is the leaf pulp or mucilage, aloe latex, commonly referred to as aloe juice, is a bitter yellow exudate from the pericyclic tubules just beneath the outer skin of the leaves. Extracts of aloe gum effectively increases glucose tolerance in both normal and diabetic rats. Treatment of chronic but not single dose of exudates of Aloe barbadensis leaves showed hypoglycaemic effect in alloxanized diabetic rats. Single as well as chronic doses of bitter principle of the same plant also showed hypoglycemic effect in diabetic rats. This action of Aloe vera and its bitter principle is through stimulation of synthesis and/or release of insulin from pancreatic beta cells. This plant also has an anti-inflammatory activity in a dose dependent manner and improves wound healing in diabetic mice. 1.2.6 Azadirachta indica: (Neem) Hydroalcoholic extracts of this plant showed antihyperglycemic activity in streptozotocin treated rats and this effect is because of increase in glucose uptake and glycogen deposition in isolated rat hemidiaphragm. Apart from having anti-diabetic activity, this plant also has antibacterial, antimalarial, antifertility, hepatoprotective and antioxidant effects. 1.2.7 Caesalpinia bonducella Caesalpinia bonducella is widely distributed throughout the coastal region of India and used ethnically by the tribal people of India for controlling blood sugar. Both the aqueous and ethanolic extracts showed potent hypoglycemic activity in chronic type II diabetic models. These extracts also increased glycogenesis thereby increasing liver glycogen content. Two fractions BM 169 and BM 170 B could increase secretion of insulin from isolated islets. The aqueous and 50% ethanolic extracts of Caesalpinia bonducella seeds showed antihyperglycemic and hypolipidemic activities in streptozotocin (STZ)-diabetic rats. The antihyperglycemic action of the seed extracts may be due to the blocking of glucose absorption. The drug has the potential to act as antidiabetic as well as antihyperlipidemic.

1.2.8 Capparis deciduas This is found throughout India, especially in dry areas. Hypoglycemic effect was seen in alloxanized rats when the rats were fed with 30% extracts of Capparis decidua (C.decidua) fruit powder for 3 weeks. This extract also reduced alloxan induced lipid peroxidation significantly in erythrocytes, kidney and heart. C. decidua was also found to alter superoxide dismutase and catalase enzyme levels to reduce oxidative stress. C. decidua additionally showed hypolipidaemic activity .

1.2.9 Coccinia indica Dried extracts of Coccinia indica (C. indica) (500 mg/kg body weight) were administered to diabetic patients for 6 weeks. These extracts restored the activities of enzyme lipoprotein lipase (LPL) that was reduced and glucose-6-phosphatase and lactate dehydrogenase, which were raised in untreated diabetics. Oral administration of 500 mg/kg of C. indica leaves showed significant hypoglycemia in alloxanized diabetic dogs and increased glucose tolerance in normal and diabetic dogs. . 1.2.10 Eugenia jambolana: (jamun) In India decoction of kernels of Eugenia jambolana is used as household remedy for diabetes. This also forms a major constituent of many herbal formulations for diabetes. Antihyperglycemic effect of aqueous and alcoholic extract as well as lyophilized powder shows reduction in blood glucose level. This varies with different level of diabetes. In mild diabetes (plasma sugar >180 mg/dl) it showsreduction, whereas in moderate (plasma sugar >280 mg/dl) and severe diabetes (plasma sugar >400 mg/dl) it is reduced to 55.62% and 17.72% respectively. The extract of jamun pulp showed the hypoglycemic activity in streptozotocin induced diabetic mice within 30 min of administration while the seed of the same fruit required 24 h. The oral administration of the extract resulted in increase in serum insulin levels in diabetic rats. Insulin secretion was found to be stimulated on incubation of plant extract with isolated islets of Langerhans from normal as well as diabetic animals. These extracts also inhibited insulinase activity from liver and kidney. 1.2.11 Mangifera indica: (Mango) The leaves of this plant are used as an antidiabetic agent in Nigerian folk medicine, although when aqueous extract given orally did not alter blood glucose level in either normoglycemic or streptozotocin induced diabetic rats. However, antidiabetic activity was seen when the extract and glucose were administered simultaneously and also when the extract was given to the rats 60 min before the glucose. The results indicate that aqueous extract of Mangifera indica possess hypoglycemic activity. This may be due to an intestinal reduction of the absorption of glucose.

1.2.12 Momordica charantia: (bitter gourd) Momordica charantia is commonly used as an antidiabetic and antihyperglycemic agent in India as well as other Asian countries. Extracts of fruit pulp, seed, leaves and whole plant was shown to have hypoglycemic effect in various animal models. Polypeptide p, isolated from fruit, seeds and tissues of M. charantia showed significant hypoglycemic effect when administered subcutaneously to langurs and humans. Ethanolic extracts of M. charantia (200 mg/kg) showed an antihyperglycemic and also hypoglycemic effect in normal and STZ diabetic rats. This may be because of inhibition of glucose-6-phosphatase besides fructose-1, 6-biphosphatase in the liver and stimulation of hepatic glucose-6-phosphate dehydrogenase activities. 1.2.13 Ocimum sanctum: (holy basil) It is commonly known as Tulsi. Since ancient times, this plant is known for its medicinal properties. The aqueous extract of leaves of Ocimum sanctum showed the significant reduction in blood sugar level in both normal and alloxan induced diabetic rats. Significant reduction in fasting blood glucose, uronic acid, total amino acid, total cholesterol, triglyceride and total lipid indicated the hypoglycemic and hypolipidemic effects of tulsi in diabetic rats. Oral administration of plant extract (200 mg/kg) for 30 days led to decrease in the plasma glucose level by approximately 9.06 and 26.4% on 15 and 30 days of the experiment respectively. Renal glycogen content increased 10 fold while skeletal muscle and hepatic glycogen levels decreased by 68 and 75% respectively in diabetic rats as compared to control. This plant also showed antiasthemitic, antistress, antibacterial, antifungal, antiviral, antitumor, gastric antiulcer activity, antioxidant, antimutagenic and immunostimulant activities. 1.2.14 Phyllanthus amarus: (bhuiawala) It is a herb of height up to 60 cm, from family Euphorbiaceae. It is commonly known as Bhuiamala. It is scattered throughout the hotter parts of India, mainly Deccan, Konkan and south Indian states. Traditionally it is used in diabetes therapeutics. Methanolic extract of Phyllanthus amarus was found to have potent antioxidant activity. This extract also reduced the blood sugar in alloxanized diabetic rats. The plant also shows antiinflammatory, antimutagenic, anticarcinogenic, antidiarrhoeal activity. 1.2.15 Pterocarpus marsupium:

It is a deciduous moderate to large tree found in India mainly in hilly region. Pterostilbene, a constituent derived from wood of this plant caused hypoglycemia in dogs showed that the hypoglycemic activity of this extract is because of presence of tannates in the extract. Flavonoid fraction from Pterocarpus marsupium has been shown to cause pancreatic beta cell regranulation. Marsupin, pterosupin and liquiritigenin obtained from this plant showed antihyperlipidemic activity. () Epicatechin, its active principle, has been found to be insulinogenic, enhancing insulin release and conversion of proinsulin to insulin in vitro. Like insulin, () epicatechin stimulates oxygen uptake in fat cells and tissue slices of various organs, increases glycogen content of rat diaphragm in a dose-dependent manner. 1.2.16 Trigonella foenum graecum: (fenugreek) It is found all over India and the fenugreek seeds are usually used as one of the major constituents of Indian spices. 4-hydroxyleucine, a novel amino acid from fenugreek seeds increased glucose stimulated insulin release by isolated islet cells in both rats and humans. Oral administration of 2 and 8 g/kg of plant extract produced dose dependent decrease in the blood glucose levels in both normal as well as diabetic rats. Administration of fenugreek seeds also improved glucose metabolism and normalized creatinine kinase activity in heart, skeletal muscle and liver of diabetic rats. It also reduced hepatic and renal glucose-6-phosphatase and fructose 1,6-biphosphatase activity. This plant also shows antioxidant activity. 1.2.17 Tinospora cordifolia: (Guduchi) It is a large, glabrous, deciduous climbing shrub belonging to the family Menispermaceae. It is widely throughout India and commonly known as Guduchi. Oral administration of the extract of Tinospora cordifolia (T.cordifolia) roots for 6 weeks resulted in a significant reduction in blood and urine glucose and in lipids in serum and tissues in alloxan diabetic rats. The extract also prevented a decrease in body weight. T. cordifolia is widely used in Indian ayurvedic medicine for treating diabetes mellitus. Oral administration of an aqueous T. cordifolia root extract to alloxan diabetic rats caused a significant reduction in blood glucose and brain lipids. Though the aqueous extract at a dose of 400 mg/kg could elicit significant antihyperglycemic effect in different animal models, its effect was equivalent to only one unit/kg of insulin. It is reported that the daily administration of either alcoholic or aqueous extract of T. cordifolia decreases the blood glucose level and increases glucose tolerance in rodents.

1.3.3 Origin and Distribution The original home of Syzygium cumini is India or the East Indies. It is found in Thailand, Philippines, Madagascar and some other countries. The plant has been successfully introduced into many other tropical countries such as the West Indies, East and West Africa and some sub tropical regions including Florida, California, Algeria and Israel. [Ross. I. A, 2003]

1.3.4 Phytochemistry Phyto is the Greek word for plant. There are many families of phytochemicals and they help the human body in a variety of ways. Phytochemicals may protect human from a host of diseases. Phytochemicals are non nutritive plant chemicals that have protective or disease preventive properties. Fruit of Syzygium cumini contains Malic acid is the major acid ( 0.59 of the wt of fruit), a small quantity of oxalic acid is also reported to be present. Gallic acid and tannins account for a stringency of the fruit. The purple colour of the fruit is due to presence of cyaniding diglycosides. Fruit contain sugar (8.09%), non-reducing sugar (9.26%) and sulfuric acid (1.21%) [Kapoor, L. D, 2001]. Glucose, Fructose, mannose and galactose are the principal sugars [Williamson, E. M, 2002]. The mineral constituents reported to be present ( mg/100g of edible pulp ) are Ca, 15, Mg, 35: P,15 (phytin P,2); Fe, 1.2 ( ionisable Fe, 0.1) ; Na, 26.2; K 55; Cu, 0.23; S,13 and Cl,8. The vitamins present ( in 100g. edible pulp) are vit.A, 80 IU; thiamine,0.03 mg, riboflavin,0.01mg; nicotinic acid , 0.2 mg; vit.C, 18 mg; choline,7 mg; Folic acid, 3 g [Williamson, E. M, 2002]. Seed contains a glucoside jamboline, a new phenolic substance, a trace of pale yellow essential oil, chlorophyll, fat, resin, albumen, tannins (19%), Phenolic such as ellagic acid, gallic acid (1-2%), caffeic and ferulic acids and derivaties, guaicol, resorcinol dimethyl ether and corilaginin [Williamson, E. M, 2002]. The seeds are fairly rich in protein and calcium. Monoterpenoids like -pinene, terpinene, terpinolene, borbeneol, -phellandrene,a-terpineol and eugenol [Williamson, E. M, 2002] and flavonoid such as rutin, quercetin.11 -sitosterol also present syzygium cumini seed [Gupta. D. R. & Agrawal, S. K, 1970]. Oil of Syzygium cumini found to contain lauric (2.8%), myristic (31.7%), palmitic (4.7%), stearic (6.5%), oleic (32.2%),linoleic (16.1%), malvalic (1.2%) and vernolic (3%) acids [Daulatabad C.M.J et.al, 2006]. Novel compounds such as 5,6-dihydroxy-3-[(4-hydroxy-6(hydroxymethyl)-3,5-di-3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2hydroxy-2-pyranyl)oxy]2-methoxy-10,13 dimethylperhydro cyclopenta [a] phenanthren-17-yl (phenyl) methyl acetate, 3,15dihydroxy androstene [16, 17-C](6methyl , 2-1,3- dihydroxy-1-propene) H pyran and 3-hydroxy androstane [16,17- C](6methyl, 2-1-hydroxy isopropene-1-yl) 4,5,6 H pyran [Shankar,M. B et.al, 2007]. Stem bark of Syzygium cumini contains betulinic acid, -sitosterol, friedelin, epi-friedelanol, and new ester of epi-friedelanol (eugenin) [Sengupta, P. and Das, P. B, 1965]. It also contains sitosterol-D-glucoside, kaempferol-3-oglucoside, quercetin,[Bhargava, K. K , et.al, 1974] myricetin, astragalin and gallic acid.[Williamson, E. M, 2002]. Gupta and Sharma isolated sitosterol,betulinic acid and crategolic (maslinic)acid and also detected n-hepatcosane, nnonacosane, n-hentriacontane ,n-octacosanol, n-triacontanol and ndotricontanol by GLC and sugars glucose, fructose ,acidsoxalic,

citric,glycolic acids and aminoacids glycine, alanine, tyrosine and leucine by co-paper chromatography in the leaves of E.jambolana [Gupta, G. S., Sharma, 1974]. Quercetin (0.0085%), myricetin ( 0.023%), myricitrin (0.009%),myricetin 3-0-( 4-acetyl ) a-L-rhamnopyranoside (0.059%) also found to contain in E.jambolana leaves [Timbola, A. K, et.al, 2002]. A complicated mixture of polyphenols such as gallic acid, methylgallate, kaempferol, ellagic acid, 3-0-methylellagic acid, myricetin 4-methyl ether 3-0-a-Lrhamnopyroside (querecet), kaempferol 3-0--Dglucuronopyranoside, myricetin 3-0--D-glucaronopyranoside, ellagitannin,nilocitin and two acylated flavonol glycosides such as mearnsetin 3-0-(4-O-acetyl )- a-L rhamnopyranoside and myricetrin 4-0- acetyl-2-0-gallate reported in E. jambolana leaves [Mahmoud, I. I, et.al., 2001]. Steam distillation of leaves give an essential oil with pleasant odour. The oil contains terpenes, 1limonene and dipentene. Oleanolic acid, erategolic acid (maslinic acid), flavonoids - isoquercitrin, quercetin, kaempferol and myricetin were found in the flowers of Syzygium cumini [Nair, A. R. and Subramanian, S.S, 1974]. 1.3.5 Traditional and Medicinal Uses Entire plant of Syzygium cumini such as seed, fruit, leaves, flower, bark used in folk medicine. Charaka used seeds, leaves and fruits in decoctions for diarrhoea and the bark as an astringent. Sushruta prescribed the fruit internally in obesity, in vaginal discharges and menstrual disorders, cold infusion in intrinsic haemorrhage [Khare, C. P, 2004]. The bark is astringent, its juice is given (56112 ml ) doses in chronic diarrhoea, dysentery, menorrhagia. Decoction of the bark is an efficacious mouth-wash and gargle for treating spongy gums, stomatitis, relaxed throat and other diseases of mouth. Bark also used for inflammation of skin. The bark is used in dyeing and tanning and for colouring fishnets. According to Ayurveda, its bark is acrid, sweet, digestive, astringent to the bowels, anthelmintic and in good for sore throat, bronchitis , asthma , thirst, biliousness, dysentery, blood impurities and to cure ulcers [Kirtikar. K.R., Basu, B. D, 1975]. The juice of Jambu, Amra and Amalaka leaves mixed with goat milk and honey prescribed in diarrhoea with blood [Khare, C. P, 2004]. Leaf juice is taken orally to treat diabetes. The juice is taken mixed with milk every morning. Fresh leaf juice is taken orally for stomach pain [Bhandary, M. J, 1995]. Syrup prepared from the juice of the ripe fruit is a very pleasant drink. Syrup or vinegar prepared from the ripe fruit is useful in spleen enlargement and efficient astringent in chronic diarrhoea [Nadkarni, K. M, 1944]. Hot water extract of dried fruits is used for stomach ulcers [Deka, L et.al., 1983], reduce acidity and for diabetes [Jain, S. R.and Sharma, S. N, 1967]. The ethanolic extract of Syzygium cumini seeds decreased blood sugar level in alloxan induced diabetic rats [Singh, N. and Gupta, M., 2007]. Seed powdered in combination with mango kernels were administered with card to overcome problems of diarrhoea and

dysentery, enlargement of spleen and as diuretic in scanty or suppressed urine [Sagrawat, H, et.al, 2006] and seed also having antibacterial activity. Thus, Syzygium cumini having pharmacological activity like anti-diarrhoel, antioxidant, gastro protective, anti-allergic, astringent, analgesic, anti-inflammatory, anti-plaque, antimicrobial but the most important activity is anti-diabetic. The Present work concentrates on the anti-diabetic activity of the Syzygium cumini. \