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Buccal Epithelial Smear and Barr Body

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Cell Biology and Genetics Cell Cycles Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain Meiosis in Grasshopper Testis (Poecilocerus Pictus) Mitosis in Onion Root Tip (Allium Cepa) Differential Staining of Blood Buccal Epithelial Smear and Barr Body Vital Staining of DNA and RNA in Paramecium Induction of Polyploidy Mounting of Genitalia in Drosophila Melanogaster Mounting of Genitalia in the Silk Moth Bombyx Mori Mounting of the Sex Comb in Drosophila Melanogaster Mounting of the Mouth Parts of the Mosquito Normal Human Karyotyping Karyotyping Black and White Film Development and Printing for Karyotype Analysis Study of Drumsticks in the Neutrophils of Females Study of the Malaria Parasite Vital Staining of DNA and RNA in Paramecium Sex-Linked Inheritance in Drosophila Melanogaster Preparation of Somatic Chromosomes from Rat Bone Marrow Chromosomal Aberrations Study of Phenocopy Study of Mendelian Traits Estimation of Number of Erythrocytes [RBC] in Human Blood Estimation of Number of Leucocytes (WBC) in Human Blood Culturing Techniques and Handling of Flies Life Cycle of the Mosquito (Culex Pipiens) Life Cycle of the Silkworm (Bombyx Mori) Vital Staining of Earthworm Ovary Culturing and Observation of Paramecium Culturing and Staining of E.coli (Grams Staining) Breeding Experiments in Drosophila Melanogaster Preparation of Salivary Gland Chromosomes Observation of Mutants in Drosophila Melanogaster ABO Blood Grouping and Rh Factor in Humans Determination of Blood Group and Rh Factor Demonstration of the Law of Independent Assortment Demonstration of Law of Segregation

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The study of the Barr body from the (female) smear of Buccal epithelial cells. Materials Buccal epithelial cells Giemsa stain Carnoys fixative Slides Cover slip Microscope, etc. Procedure 1. Gently rub the inside of the cheek with a flat rounded piece of wood and transfer the scraping over a clean glass slide. 2. Then, made a thin film of cells on the slide and keep them for air-drying. 3. Air-dried smear was kept in Carnoys fixative for 3035 minutes. 4. Then, the Giemsa stain was poured and allowed to stand for 2025 minutes. 5. After staining, the slide was washed with distilled water to remove the excess stain. 6. Finally, the slide was kept for air-drying and then observed under the microscope.

Observations We found that very lightly stained cells are scattered here and there in the smear. In the cells, violet-Barr bodies are observed inside a pink nucleus. A Barr body is nothing but an inactivated (heterochromatinized) X chromosome. It was first observed by Murray Barr in 1949. It is found only in female cells, because in those 1 X chromosome is enough for metabolic activity. It is absent in male somatic cells, because there only 1 X chromosome is present, which is in an active state. Precautions The smear or film should be uniform and thin over the glass slide so that the cells will not overlap each other.

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