Shin Yoshioka1, R yosuke Sakumot o 2, Kiyosh i Okuda1.

1
Okayama University Graduate School of Natural Science and Technology, Okayama, Japan; 2National instit ute of Agrobiological S ciences, Tsukuba, Japan
The hippo signaling pathway controls organ size in mammals through the cell proliferation and apoptosis. It has been demonstrated that the cell density regulates hippo sign aling pathway. Recently,
we found that yes-associated protein 1 (YAP1), one of the target of hippo signaling pathway and which an indicator of cell density, expresses in bovine corpus luteum (CL) and lo calizes in nuclear throughout
the estrous cycle. Furthermore, nucleic expression of YAP1 was higher at the early lu teal st age (Days 2-3 after ovulatio n) than at the oth er lu teal stages and gradually decreased toward the regression stage
(Days 18-19 after ovulation). Since YAP1 is known to be expressed in nuclear under the low cell density, the C L seems to be low cell density at the early luteal stage and increases cell density following C L
growth. However, roles of cell density in CL are not known. The CL is a transient endocrine gland form ed from a ruptured follicle after ovulation and the bovine CL is to produce not only progesterone (P 4)
but also est radiol (E2). P 4 plays ess ential roles for the establis hm ent and maintenance of pregnancy in mammals. Alth ough follicular E2 is known to in duce cell proli feration of uterine epithelial cells, a
physiolo gi cal rol e of lu teal E2 is not well understood in cattle. To investigate whether cell density affects th e C L functions, we examined 1) P 4 and E2 production throughout the estrous cycle in the bovi ne
C L tissue and 2) effects of cell density on P4 and E2 production with or without the lu teinizing horm one (LH) in the cultured bovine luteal cells. The CL tissues were collected at the early (Days 2-3),
developing (Days 5-6), m id (Days 8-
12) and late (Days 15-17) luteal stages. B oth P4 and E2 productions (per gram ti ssue) were higher at the early luteal stage th an at the ot her luteal stages in the medium of cultured bovine C L tissue and this
ability of CL decreased toward the regression stage. C ultured luteal cells were exposed to LH (10 ng/ml) for 24 h, and P4 and E2 concentrations in the cultured medium were determ ined by EIA. LH stimulated
P4 production but not E2 production in cultured luteal cells under the both low and high cell density. Interestingly, low cell densit y up-regulated E2 production (about 4 fold) but did not affect P4 production in
the cultured bovi ne luteal cells. To investigate how E2 production is up- regulated by low cell density, we examined th e effect of lo w cell densit y on th e expression of aromatase, an enzym e responsible for the
biosynt hesis of estrogen, in the cultured bovine luteal cells. The aromatas e mR NA level was determ ined by real-time RT-PCR. The low cell density did not affect aromatas e expression in mRNA level. In
conclusion, since E2 production by bovine luteal tissue was higher at th e early luteal stage (a timing of luteal growth), and E2 production by bovine luteal cells was increased by low cell density, the increased
E2 production by low cell densit y may induce the luteal cell proliferation in the bovine C L at the early stage. However, it is still not clear how E2 production  is regulated by cell density in bovine C L.

851.   Publi cation Rates of Abst racts on Male Infertility Presented at th e Society for th e Stud y of Reproduction (S S R) Annual Meetings Between 1999 and 2009.
Burak Ozkosem 1.
1
Urology Research Laborato ry, McGill Universit y, Montreal, Quebec, C anada
The conversion of scientific knowledge in fertility research from bench si de discovery into bedside practice remains less than ideal. A key step in this conversion occurs when presentations from
major reproduction meetings such as th e Society for th e Study of Reproduction (SSR) are published in peer-reviewed literature. To our knowledge, no previous st udies have looked at the rate of publication of
these studies focusing on male in fertility in a peer-reviewed journal. Our objective was to define the fate of abstracts presented with male infertility focus at the annual meetings of the Society for the Stu dy of
Reproduction (SSR) between 1999-2009 and to describe the factors predicting and influencing ultimate publication in scientific journals. All abstracts presented at the SSR annual m eetings from 1999 to 2009
were surveyed to find male infertility abstracts. P ublication status as of May 2013 was assessed usin g th e Web of Science (ISI) database and Harzing’s Publish   or P erish search engin es. Abstract parameters
were logged including number of authors, country of origin, clinical or basic research, and institution. Univariate and m ul tivariate analyses were perform ed to determ ine the association between abstract
characteristics and subsequent full publication. We assessed the abstract publication rates, journal impact factors and citations for each published abstract usin g multivariable logistic regression modeling. A
total of 109 male infertility abstracts were presented from 1999 to 2009. 41(38%) of these were published in peer-reviewed journals at 3.8 years. On m ulti variate analysis, the predictors of publ ication
included the num ber of authors (OR 1.3, C I 1–1.4, P
< 0.01) and basic science research (OR 5.8, CI 2.7–12.6, P < 0.01). Median impact factors for subsequent publications varied from 4.1 (interquartile range, 3.6–11. 1, P < 0.01). C linical science was less likely
to be published compared with basic science. Approxim ately two- fifths of abstracts on m ale infertility presented at SSR m eetings are subsequently published in peer-reviewed journals. Basic science content
and the m eeting at which the abst ract was present ed are ass ociated with publi catio n. Our findings su ggest that efforts to understand the difficulties to publication and to facilitate the rapid delivery of new
knowledge are needed to speed up the conversion of scientific discovery into clinical practi ce.

852.   Rep roductive Neu roendocrin e Disruptio n in Male Goldfish Exposed to Vinclo zolin.
Mahdi Golshan 1, Azadeh Hatef2, Ava Zare3, M agdalena S ocha4, Miroslaw Sokolowsk i - Mikolajczyk 4, P ascal Fontaine5, Hamid. R Habibi 3, Sayyed Mohammad Hadi Alavi1.
1
Faculty of Fis heries and Protection of Waters, vodnany, C zech Republic; 2University of S askatchewan, S askatoon, Canada; 3University of Calgary, C algary, Canada; 4Agricultural Universit y in Krakow,
Krakow, Pol and; 5Universite de Lorraine, Nancy, France
In m amm als, vi nclozolin (VZ) is known as anti -androgen, which causes m ale in fertilit y m ediated by disruption in androgenesis via androgen receptor antagonism. In fish, the VZ effects on
reproductive functions are largely unknown and existed literature show som ewhat contradictory results. To understand VZ adverse effects on male reproduction, mature goldfish (Carassius auratus) were
exposed to three nominal VZ concentrations (100, 400, and 800 μg/L) for 30 days. Sperm production and motility kinetics were evaluated. Alternations in

379