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Microbiology Final Review Media

Selective (chooses what will grow or not grow; like the salt in the agar will chose what will grow or not grow)/ differential (can show you different colors) if something will grow or not; may grow different colors; may matabolize one of the compounds and turn a different color)/ general (just want it to grow, dont know what we have just want it to grow)/ enriched (provides nutrients; provides something in the agar that the bacteria need to grow) Components that allow for S/D/E

Test for?

pH or other Examples of results indicator

Lab #

Trypticase soy Agar (TSA)

GENERAL

Microbiology Final Review Blood Agar (BAP) ENRICHED The iron Hemolysis (breaking down RBC by endotoxin) Alpha: Green/Yellow NOT clear Beta: CLEAR/see through/transparent Gamma: didnt do anything, bacteria just grew. Mannitol Salt Agar (MSA) **Selective agent 7.5% Salt; Differential mannitol and phenol red Differential agent: mannitol & phenol red Mannitol tests for: fermentation of sugars Phenol red tests for: Ph Phenol: pH indicator low pH yellow neutral pH orangish red high pH pinkish red -Staphylococcus aureus yellow (low pH) & (+) for fermentation - Staphylococcus epidermis pink (high pH) & (-) for fermentation Staph and Strep *Strep Pyogenese

Phenol Red Broth (PRB) Triple sugar Iron (TSI) SIM (Sulfur [indole] motility) Endol? If it can ferment can carry out metabolic processes without oxygen NEGATIVE: YELLOWISH POSITIVE: BLACK

Microbiology Final Review MacConkey (MAC) Selective & Differential Bile Salts: - selective - supports gram negative Neutral Red: - differential - pH indicator - low pH red/purple/pin k colonies - neutral colorless Lactose: - differential - (LF) - lactose fermentation - (NLF) - no lactose fermentation Urea slant Citrate Mueller-Hinton (MH) Looking to see if they produce urease Yellow went to hot pink if there was presence

Microbiology Final Review Other Tests: Test For/ How it works Positive BUBBLES if POSITIVE Negative

Catalase (if the bacteria have With hydrogen peroxide; the enzyme present the bubbles) bubbled on slide (production Dont use for gram negative, of gas and bubbles) just positive Coagulase

Bacitracin/optochin

Kirby Bauer

Oxidase (use to look for presence of cytochrome 3: protein)

Put the discs to inhibit the growth of bacteria Looking for inhibition of growth with the measure of the diameters) PAPER; turned purple/black for positive

Is the inhibited part if it inhibits it is is positive

Enterotube: **COMPOUND MICROSCOPE**

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