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DETECTION OF PATHOGEN MICROBES IN FEMALE’S GENITAL TRACT AND

ANTIBIOTIC’S SENSITIVITY IN THE WIDER AREA OF ATHENS

P. Papalexis1, P. Christopoulos2, O. Papatzitze3, E. Markanastasaki3, C. Taskou3,


G. Iatrakis , E. Kalkani , C. Papadias
3 3 2

1Medical
School of Athens, National & Kapodistrian University of Athens
2 2nd Department of Obstetrics & Gynecology, Aretaieio Hospital, University of Athens Medical School
3 National Technological Educational Institute of Athens (TEI-Athens), Departments of Medical Laboratory Technologists

and Obstetrics

Introduction:
It is well-known that some factors, such as the disturbances of the normal commensal
flora of the vagina, the alterations of local pH due to topical irritations, stress, the
cyclical endometrial changes, the decreased immune protection and sexually
transmitted microbes, cause important infections in the vagina, the cervix and the
vulva, as well as infertility and chronic inflammation that leads to serious damage in
some organs. Our purpose is to identify pathogen microorganisms in women’s genital
tract, as common as Mycoplasmas, Chlamydia trachomatis, aerobic and anaerobic
bacteria.
Materials & Method: We studied 200 women from 3 years to 58 years old (mean
age: 24,5), from 1 May 2005 to 14 July 2008, located in the wider area of Athens at a
three years perspective study and we examined clinical specimens of all women’s
vagina using direct microscopy in untreated wet films. Then we detected the possible
pathogen microbes by cultivation in aerobic and anaerobic conditions, in appropriate Chlamydial cervicitis
culture media. The detection of Mycoplasma hominis (Mh) and Ureaplasma
Urealyticum (Uu) has been done by inoculation of the vaginal secretion in A7 Ureaplasma urealyticum
appropriate culture media and the detection of Chlamydia trachomatis (D-K serovars) & Mycoplasmas culture in UMMT
was performed by sensitive immunochromatography combined with the determination mycofast medium
of IgA, IgM and IgG antibodies in the blood serum. After the identification of the
microbes, we examined the sensitivity of many antibiotics, separately for the
aerobic,the anaerobic bacteria or
fungi and the Mycoplasmas.
Results: The antibiotics with the most sensitivity in both aerobic and anaerobic
bacteria are in order: 1) Ofloxacin 2) Cefuroxime 3)
(Tabrin) (Radaxef) Cefprozil
(Procef)
Candida albicans culture in
4) Amikacin 5) Trimeth.+Sulfamicole Sabouraud agar
(Briklin) and (Septrin) and Norfloxacin
Ciprofloxacin (Norocin)
Conclusion: (Ciproxin)
The frequent and careful examination from the Gynecologist, the observance of all
hygiene rules, the correct and strict use of condom in order to avoid sexually
transmitted microbes, the thorough washing and pressing of the underwear of the
women, and the correct differential diagnosis of the infection cause, from the normal
microbial flora, as well as the right treatment with new antibiotics that eliminate a
variety of microbes in the woman’s urogenital tract, consist the essential strategies
for woman’s health. E.coli

MICROBES PERCENTAGE ANTIBIOTIC


AEROBIC CULTURE IN A TOTAL OF 200 WOMEN
C. albicans From the anaerobic culture
OF CHOICE IN
we found Bacteroides fragilis ORDER
26
E.coli& C.
albicans in 22 out of 200 women Mycoplasma 2,5 % Doxycycline,
53
not hemolytic
(11%) and Streptococcus hominis Ofloxacin,
Roxythromycin
18 streptococcus saprophyticus in 15 (7,5 %).
S.epidermidis In some cases the women Ureaplasma 13 % Doxycycline,
urealyticum Ofloxacin,
37 have to take an antibiotic’s Roxythromycin
spectrum.
Micrococcus
61
14
References: Chlamydia 15 % Doxycycline,
David Greenwood, Richard Slack C.B.: Medical Microbiology, 16th edition, trachomatis Tetracycline,
Churchill Livingstone editions, United Kingdom,2003, Robin Reid, Fiona
Erythromycin
Roberts: Pathology illustrated, 6th edition, Churchill Livingstone editions,
in babies
London 2005, www.ncbi.nlm.nih.gov, www.chlamydiae.com,
www.clinical-mycology.com, http://panacea.med.uoa.gr/.