NEURITEOUTGROWTHASSAY

QUANTIFICATIONOFNEURITELENGTH,BRANCHING,...
DOMAIN:neurology,brainresearch

The Neurite Outgrowth Assay (NEO) is a fully automated software package for quantifying neurite outgrowth of neuronal cell cultures (cell lines and primary cells). Regeneration of neurons represents a promising strategy for drugs targeted against neurodegenerative disorders such as Alzheimers and Parkinsons disease. Novel therapies focus on identifying molecules that affect the differentiation of neurons and neurite outgrowth so the automated measurement and analysis of neuronal cells is an essential tool for neuroscience researchanddrugdiscovery. The DCILABS software package automatically quantifies the different aspects of the cells and the neurites by a variety of output features including neurite length, number of neurites/cell, number of positive cells, branching, The software is able to analyse nonstained samples,fluorescentsamplesand/oracombinationofboth.Theimage importertoolallowsusingimagesfromdiversimagingsystemsbutyou can also send your samples to our service lab, where we can record andanalysethem.

Includesapowerfulvisualisationmoduleforquickreviewofanalyseddata. Assuresexcellentneuritedetectionandquantificationbyacombinationofastateoftheartimaging algorithmandanextensivepanelofcellandneuriterelatedparameters. Algorithmsettingscansimplybeadjustedtoassurecorrectanalysisofawiderangeofcelltypesand images.

DigitalCellImagingLabsMwamiMutaradreef18,3140Keerbergen,Belgium [TEL]+322652460[FAX]+3232652245info@dcilabs.comwww.dcilabs.com

 NEURITEOUTGROWTHASSAY (NEO)
Usage Imageandsystem requirements usedfor application domain keywords imageinput Quantificationofneuriteoutgrowthofneuronalcellcultures(cell linesandprimarycells). Neurology,brainresearch,drugdevelopment Neuriteoutgrowth,AlzheimersandParkinsonsdisease. Imagesof2Dcellcultures(bothnonfluorescentaswellas fluorescentimages) ImageimportersareavailableforTIFFfiles,MAIAScientific,Incell, NikonandAndormicroscopes. Specificimporterscanbedevelopedonrequest. APCrunningWindowsXP/Vista/7(32or64bit),2GBRAM recommended. Alargemonitorwithaminimumresolutionof1280x1024. Celllinesorprimarycellculturesofneuronsormixedpopulations 1channelassay:nonstainedsamples 2channelassays:nucleusstain+tubulinstain 3channelassay:nucleusstain+tubulinstain+neuronalmarker Totalnumberofcells/nuclei. cellswithatleastoneneurite/overlappingwithtubulin Numberofneurites Numberofbranchingpoints Totallengthofallneurites Totalneuritearea Averageneuritelengthpercell/nucleus Averageneuritelengthperpositivecell/nucleus Averagenumberofneuritespercell/nucleus Averagenumberofneuritesperpositivecell/nucleus Averagenumberofbranchingpointspercell/nucleus Averageneuritelengthperbranchingpoint Averagenumberofbranchesperneurite Numberofnegativenuclei(toindicatenonneuronalcells; measuredin2or3channelassayonly) Areaofthenucleus Diameterofthecell

imageimporters minimumSystem Requirements Samplerequirements sampletypes staining

Output

outputfeatures

DigitalCellImagingLabsMwamiMutaradreef18,3140Keerbergen,Belgium [TEL]+322652460[FAX]+3232652245info@dcilabs.comwww.dcilabs.com