Effect of temperature on enzyme reactions You studied the enzyme reaction 2 H2O2 Hydrogen peroxide catalase enzyme 2 H2O

Water + O2 ( g ) Oxygen gas

practically . This practical aims to show what is the effect of temperature on the enzyme and to compare that with the effect of temperature on the same reaction but with a chemical ( non enzyme ) catalyst .

Procedure Take the injection syringe out of the socket . Set up the apparatus ( if it has not been done for you ) : Plunger

Injection syringe Vinyl / rubber tube 5.0 cm

3

Gas syringe

Nozzle Needle socket V Bung

Conical flask

so that the vinyl rubber tube is both : i ) straight ( like in the diagram ) and ii ) will not leak gas ( unlike in the diagram ! ) when the bung is in the top of the conical flask . Now take the bung out of the conical flask . Set the gas syringe so that V = 0.00 cm3 . Set the injection syringe at 0.00 cm3 . Measure 40 cm3 1.25 vol hydrogen peroxide ( CARE ! GOGGLES ! ) in a 50 cm3 measuring cylinder and put it into the conical flask . This should already have been done for you and the conical flask of hydrogen peroxide put in a water bath ( except the reaction that is being done at room temperature ) . Take the hydrogen peroxide that is in the 35C - 40 C . first . Put the bung in the top of the conical flask so that it will not leak gas . Draw up 5.00 cm3 of potato extract from the beaker that is at your selected temperature into the injection syringe . Put the injection syringe nozzle into the needle socket BUT DO NOT PUSH THE PLUNGER YET . Get ready to start the stopwatch . Get ready to read the volume V on the gas syringe . Get ready to record the volume V after every 20 seconds in a table : Temperature = Time / s Volume / cm
3

20

40

60

There is no need to continue the timing and recording beyond 60 seconds for each experiment . Continue your experiments in the order : 20 C 5C 60 C

Temperature = Time / s Volume / cm

3

20

40

60

Temperature = Time / s Volume / cm

3

20

40

60

Temperature = Time / s Volume / cm

3

20

40

60

Processing the results

Construct a new table : Temperature / C V20 / cm3 ( V20 5.0 ) / cm3 Initial rate / cm3 s -1

V20 = the volume of gas V produced after 20 seconds in that experiment ( V20 5.0 ) is the volume of oxygen gas actually produced . Remember how you injected 5.0 cm 3 of potato extract and it would be unfair to count that as oxygen gas . Initial rate = ( V20 5.0 ) / 20 because you measured the volume after 20 seconds . Use these results to draw a graph of initial rate ( y axis ) against temperature ( x axis ) .

Interpreting results Use your graph to try to estimate what the rate of reaction would have been at 60 C .___________ Your result for 60 C should be very , very much less than that . What was catalyzing the reaction at 5 / 20 / 35 C ?______________________________________

So what has happened to the catalase enzyme at 60 C ? _________________________ An enzyme is what type of a biochemical ? ____________________________ The correct action of an enzyme depends in it having the correct____________________ Heating the enzyme causes ____________________________________ to be broken and so when it has been heated the enzyme has the wrong shape to catalyse the reaction .