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Code No: R05222303

Set No. 1

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 MOLECULAR BIOLOGY (Bio-Technology)

Time: 3 hours

Max Marks: 80

Answer any FIVE Questions

All Questions carry equal marks

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1. What does “right handed helix” & “left handed helix” mean? Explain taking

suitable examples.

[16]

2. How does rolling circle replication produce multimers of a replicon?

[16]

3. How is the lagging strand synthesis different from the leading strand synthesis in

DNA replication?

[16]

4. Justify the statement “there are different types of rRNA found in ribosome” [16]

5. Using suitable examples explain how they are acting as inhibitors of transcription.

[16]

6. What is meant by “Wobble hypothesis”? Explain its connection to the genetic

code.

[16]

7. How is the initiation of translation process occurring in prokaryotes and eukaryotes?

Elaborate briefly.

[16]

8. Write short notes on

[2 × 8 = 16]

(a)

Thiamine dimmers and photo reactivation

(b)

Excision type of DNA repair

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Code No: R05222303

Set No. 2

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 MOLECULAR BIOLOGY (Bio-Technology)

Time: 3 hours

Max Marks: 80

Answer any FIVE Questions

All Questions carry equal marks

⋆ ⋆ ⋆ ⋆ ⋆

1. What does “right handed helix” & “left handed helix” mean? Explain taking

suitable examples.

[16]

2. Using neat diagrams explain a circular chromosome undergoing bi-directional semi

conservative replication.

[16]

3. Explain the process of DNA replication in detail.

[16]

4. Discuss in detail the structure of the organelles functioning as sites of protein

synthesis.

[16]

5. Using suitable examples explain how they are acting as inhibitors of transcription.

[16]

6. In the genetic code, how can one justify the presence of 64 codons coding for only

20 amino acids.

[16]

7. Once a protein is synthesized, can it be modified? Justify your answer giving

suitable examples.

[16]

8. With the help of a neat diagram explain how the process of site directed mutagenesis

is done.

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[16]

Code No: R05222303

Set No. 3

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 MOLECULAR BIOLOGY (Bio-Technology)

Time: 3 hours

Max Marks: 80

Answer any FIVE Questions

All Questions carry equal marks

⋆ ⋆ ⋆ ⋆ ⋆

1. Write a note on the different forms of the double helix DNA and elaborate on “B”

form.

[16]

2. How does the bacteriophage Φ×174 replicate through rolling circle mechanism?

[16]

3. What is meant by ‘end replication problem’ in DNA synthesis? Discuss the role of

telomase in it.

[16]

4. Write about the different types of ribonucleic acids.

[16]

5. Where does the synthesis & processing of pre-rRNA occur? Explain the process.

[16]

6. Write briefly about the steps of initiation, elongation and termination in the process

of prokaryotic translation.

[16]

7. What are post transnational modifications? Give some examples.

[16]

8. How is reverse genetics approach used to study a gene function?

[16]

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Code No: R05222303

Set No. 4

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 MOLECULAR BIOLOGY (Bio-Technology)

Time: 3 hours

Max Marks: 80

Answer any FIVE Questions

All Questions carry equal marks

⋆ ⋆ ⋆ ⋆ ⋆

1. Explain how GC base pair content of a ds DNA affects the melting temperature

Tm.

[16]

2. With respect to DNA replication, explain

(a)

Origin of replication

(b)

Replication fork

(c)

Bidirectional replication

(d)

Semi-conservative replication.

[4 × 4 = 16]

3. How does DNA replication proceed in the 5’ > 3’ polarity parental DNA strand?

 

[16]

4. Give a step-by-step description of the process of Transcription.

[16]

5. Describe briefly about the posttranscriptional processing occurring in eukaryotic

mRNA.

[16]

6. In the genetic code, how can one justify the presence of 64 codons coding for only

20 amino acids.

[16]

7. With context to gene transfer mechanisms, explain

[2 × 8 = 16]

(a)

Transduction

(b)

Conjugation

8. Explain how base analogs and alkylating agents act as chemical mutagens in in-

duced mutations.

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[16]