Académique Documents
Professionnel Documents
Culture Documents
Dr.Aravind
Facultative Anaerobes
Lipopolysaccharide (LPS): Antigenic & Endotoxin Belong To Diverse Taxonomic Groups Cause Diseases In The GI Tract, Outside The GI Tract, Or In Both Locations.
Oxidase Negative
Non Spore-forming
Dr.Aravind
Enterobacteriaceae
Classification
Non Lactose Fermenters Lactose fermenters Escherichia Coli (Motile) Klebsella Pneumonia (Non motile) Motile and H2S producing Salmonella Proteus
Dr.Aravind
Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Enterobacteriales Family: Enterobacteriaceae Genus: Escherichia Species: E. coli
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Virulent Factors
P Pili (Pyelonephritis-associated Pili):
Binds To Galactose Dissaccharide Of UT Epithelium Most Imp In UTI Bind To The D-mannose Receptors On Epithelial Cell Surfaces Bind To Enterocytes
Type I Pili:
CFA Or BFA:
E.Coli fimbreae
Capsule (K Antigens) :
K1 Capsule In Neonatal Meningitis Antibodies And Complement Bacterial Surface, Phagocytosis
O Antigen :
Outermembrane Endotoxin
H Antigen:
Flagella Motility And Invasive Disease
Alpha-hemolysins
Pore formation on membrane, toxic to many mammalian cells. Lyse Lymphocytes, Beta-hemolysins, Inhibit Phagocytosis And Chemotaxis Of Neutrophils
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Exotoxins:
Heat-labile Enterotoxin (LT) Similar Function To The Cholera Toxin Binds Identical Ganglioside Receptors On Enterocytes Heat Stable Toxin (ST) Binding To Guanylate Cyclase Receptors On The Apical Membranes Of Host Cells, Thereby Activating The Enzyme Diarrhea. Resistant to boiling 300C Shiga toxins (verotoxin) protein synthesis by destructing 28S ribosome HUS syndrome O157:H7 serotype
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Epidemiology
Human Bowel Is Usually Colonized Within 40 Hours Of Birth. 700 Serotypes Of E. Coli Based On O, H, And K Antigens Bacteroides Species : E. Coli = 20:1 Most Common Cause Of UTI 2rd Most Common Cause Neonatal Meningitis A Leading Cause Of Infantile Diarrhea In Developing Countries Transmission:
UTI - intestinal E. coli perineal Urethra Neonatal Meningitis Birth Canal Intestinal disease contaminated Food or water
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Pathogenic e.coli
Intestinal diseases
E.coli Strains ETEC EPEC EIEC EAEC EHEC
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Rectum
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Clinical conditions
Extra intestinal infections
Urinary Tract Infection: Uropathogenic E. Coli (UPEC) Cause 90% Of The UTI pregnancy Dysuria, Lower Abdominal Pain, Increase Discharges Cystitis, Increased Frequency Of Urination, Dyspareunia Pyelonephritis: Fever, Dysuria, Radiating Back Pain, Tenderness At The Costo Vertebral Angle, Shock.
Dr.Aravind
Neonatal meningitis: 2 nd most common Transmission - Birth canal Virulent factor K1 capsular antigen Fever, irritability, constipation, poor feeding, bulging frontanella, seizures Neonatal Pneumonia: Birth canal Respiratory symptoms Septicemia
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Intestinal Diseases
Enterotoxigenic E. Coli (ETEC): Infants And Travelers To Underdeveloped Countries Or Regions Of Poor Sanitation In U.S Outbreaks By Water, Soft Cheeses, Mexican-style Foods And Raw Vegetables Infective Dose 108 Onset In 1 2 Days Virulent Factors:
CFA, LT And ST
Watery Diarrhea Similar To Cholera Syndrome Non Inflammatory, Non Invasive Adults in endemic areas evidently develop immunity Self limiting
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Virulent Factors:
Intimin, E. Coli Secretion Proteins (Esps), Tir (Translocated Intimin Receptor) A/E Lesions
Enteropathogenic Escherichia coli (EPEC) attachment to epithelial cells. The EPEC are attaching to and effacing the microvilli on the epithelial cell surface. The cells filamentous actin is rearranged at the attachment point
EPEC Pathogenesis
Dr.Aravind
Courtesy by Crankshaft
Symptoms:
Watery Diarrhea ,Severe Cramps And Progresses To Bloody Diarrhea. Fever Is Low Grade Or Absent
Hemolytic-uremic Syndrome (HUS) Thrombotic Thrombocytopenic Purpura, Renal Failure And Microangiopathic Hemolytic Anemia HUS Ensues In Approximately 5% To 10% Antibiotics may induce Shiga toxin.
Complication:
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EHEC Pathogenesis
Dr.Aravind
Courtesy by Crankshaft
Diagnosis
Extra intestinal Infections:
Specimens - bladder or CSF Culture on MacConkey agar (pink colonies) Serological tests
Intestinal infections:
Culture on MacConkey agar ( Pink colonies) EMB agar EHEC MacConkey sorbitol agar - No growth
Treatment
Extra Intestinal Infections: UTI: Ciprofloxacin, Trimethoprim, Sulfamethoxazole. Meningitis: Cefalosporins Intestinal Infections: Rehydration And Supportive Measures Trimethoprim/Sulfamethoxazole ETEC, EPEC And EIEC. EHEC: Hemodialysis Or Hemapheresis No Anti Motility Drugs
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Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Enterobacteriales Family: Enterobacteriaceae Genus: Klebsiella Species: K. pneumoniae
Klebsiella Pneumoniae
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Gram-negative Rod Shaped Bacterium Non-motile, Oxidase (-) Lactose Fermenting, Facultative Anaerobic Found In The Normal Flora Of The Mouth, Skin, And Intestines Thick Capsule-made Of Polysaccharides Virulent Factors: Capsule & Endotoxin Susceptible Pts:
VAP Hospitals Alcoholics & Diabetics Immuno-compromised Patients & Catheter
Causes Pneumonia, Meningitis And UTI Thickness And Bloody Sputum- B/C Of Thick Capsule Diagnosis: Culture, Serology Treatment: Cephalosporin's - Cefotaxime & Fluor quinolones
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