Académique Documents
Professionnel Documents
Culture Documents
l a b s
Table of Contents
3. Company Introduction 5. Yeast - Liquid Cultures 6. Yeast - Dried 7. Nutrients & Enzymes 9. Consulting
Yeast Culture Banking & Maintenance Services
11. Analytical Services 12. Vodka 14. Gin 16. Tequilla 18. Rum 19. Bourbon Whiskey 22. Rye Whiskey 24. Brandy
The company was founded by Chris White, Ph.D, following years of research and development of a library of brewers yeast strains from around the world. Chris White received an undergraduate degree in Biochemistry from U.C. Davis, where his interest in brewing was inspired by Dr. Michael Lewis and Brewing and Malting Science courses. He began homebrewing and relocated to San Diego for graduate studies in biochemistry. Chriss doctoral thesis focused on developing high cell density growth techniques for an industrial yeast strain, Pichia pastorus. Because of his passion for craft brewing, Chris began to experiment, using the same high cell density growth techniques on brewers yeast. Chris developed a process to grow pitchable quantities of pure liquid brewers yeast. This innovation caught on quickly within the brewing community and White Labs yeast developed a strong word-of-mouth reputation. White Labs Yeast Culture Collection currently has over 500 deep frozen strains from around the globe. White Labs began providing one strain of yeast for one local San Diego homebrew shop, Homebrew Mart. Today, White Labs provides hundreds of yeast strains to breweries, wineries, distilleries, and homebrew retailers all over the world.
WLP001-California Ale Yeast Suitable for vodka, gin and neutral spirits
WLP070-Bourbon Yeast
Suitable for Bourbon or other American Whiskey with barley, rye, or corn as the base grain
WLP760-Cabernet Yeast
Suitable for vodka, gin, grappa and neutral spirits
gram package.
YEAST-Dried
WLDWHISKEYAG-1KGWhiskey Turbo AG-1kg
Pure culture whiskey strain of saccharomyces cerevisiae used widely in commercial whiskey, with robust and authentic aromatics even in highalcohol fermentations. Includes the advantage of complete nutrition and amylo glucosidase. This enables both complete dextrin conversion of malt or grain for maximum yield as well as rapid fermentation to 15% abv. - very high for standard whiskey low beers.
Danstil EDV 46
Active dried strain, selected in France from a beet molasses fermentation, is also suited for fermentations of other by-products of the sugar industry. It is a fast starter, has high osmotolerance (up to 20%), and an exceptional aptitude for alcohol conversion. Available in 500 gram package.
K1
Lalvin V1116 (K1) is an active dried yeast for use in rum, brandy and fruit brandies. Isolated in 1972 by Pierre Barre of the INRA Montpellier. K1 is also used in white wines, ice wines, roses and basic red wines. Available in 500 gram packages.
71B
Lalvin 71B is an active dried yeast recommended for use in vodka, gin, and neutral spirits. Also used in nouveau wines, 71B was isolated by the INRA (National Agricultural Research Institute) in Narbonne, France. Available in 500 gram bags.
EC-1118
EC-1118 is an active dried yeast for use in whiskey and bourbons. It has been isolated in Champagne and its use validated by the Comit. Interprofessionnel du Vin de Champagne (CIVC). Sold in 500 gram bags.
WLDTY48-1KGTY48 - 1kg
High performance temperature and ethanol tolerant yeast capable of reaching 20%abv from pure sugar wash in 8 days. With AG enzyme to allow starch based feedstock after normal gellatination and alpha amylase treatment.
Go-Ferm
Go-Ferm provides bio-available micro-nutrients in the non-stressful environment of the yeast rehydration water. By making key minerals and vitamins available to the selected yeast at the critical beginning of rehydration, the yeasts viability increases and fermentations finish stronger. 200g services 200 gallons.
Consulting
White Labs offers expert, affordable on-site assistance for all of your fermentation needs, including: Laboratory Staff Training Laboratory Set-Up, including protocol and procedure manuals Contamination Risk Assessment/Clean-Up New Product and/or Fermentation Method Assistance Yeast Handling Yeast Propagation Facility Audit Product Development For more information, and to discuss you individual needs, please contact us at info@whitelabs.com
Fermaid K
Fermaid K is a blended complex yeast nutrient that supplies ammonia salts (DAP), alpha amino nitrogen (derived from yeast extract), sterols, unsaturated fatty acids, key nutrients (magnesium sulfate, thiamin, folic acid, niacin, biotin, calcium pantothenate) and inactive yeast. Fermaid K should be hydrated before adding to an active fermentation to avoid CO2 release and overflowing of tanks or barrels. It is available in 10 kg boxes and 2.5 kg bags.
WLN4500 ProMax
Increase the health of your yeast with Free Amino Nitrogen provided by Pro Max, a protease that releases protein from the starches in your mash.
WLN4100 Ultra-Ferm
Enzyme used to provide higher Attenuation, which completely hydrolyzes all sugars into fermentable ones. Increases alcohol yield.
WLN4400 Visco-Buster
Decrease the viscosity of mashes by using Visco-Buster to hydrolyze the Beta Glucans. Particularly useful in mashes with high percentage of corn, rye, or other non-barley starches.
WLN4300 Opti-Mash
Using Corn, Rye, Rice, Buckwheat or other non barley sources? Get better starch liquefaction with Opti-Mash, a thermostable alpha-amylase that keeps working when barley amylases quit.
while staying true to the indigenous microbiological characteristic. After obtaining a pure single-strain or mixed culture unique to the product, White Labs will maintain the strains in a stable format until needed by the client. These native strains are proprietary to the individual client, and can be provided on slants or in pure liquid cultures.
Special Projects
Always eager to stay on the forefront of fermentation science and its applications, White Labs is frequently engaging in Special Projects for clients in all areas of fermentation. Apart from beer, distilling and wine projects these include baking, coffee, cocoa, kombucha, kefir and other fermented products. Fermented foods of all sorts are continuously gaining popularity among consumers and White Labs is proud to offer custom project solutions ranging from the counter-top hobbyist to high technology research projects. The Special Project Team is here to support wherever there is fermentation involved!
Analytical Services
Catalog# Distilled Beverage Analysis
LS6646 LS3450 Alcohol by Volume
Price
$25
Quantity Required
200 mls 200 mls
$138 Distillation Profile by Gas Chromatography (Ethyl Acetate, 1-Propanol, Acetaldhyde, Isoamyl Alcohol, Isoamyl actate, Ethyl octanoate, ethyl hexanoate, acetone, ethyl butyrate, isobutyl acetate, methanol, isobutanol, 2-methylbutanol) $369 Heads, Hearts, and Tails Analysis (Distillation Profile by GC for 5 samples) Methanol by Gas Chromatography Furfural Alcohol Titratable Acidity $89 $89 $23
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Fermentation Targets
Vodka
Fermentation and Sugar Source
In the production of neutral vodkas, much depends on the selection of a suitable spirit, and the fermentation base of the spirit is less critical than the degree of purification achieved in distillation. In Russia, vodka is made from both grain and potato spirit; in the West, grain and molasses spirit are used. The selected spirits are diluted in alcoholic strength and subjected to treatment with activated carbon. For potato vodka, potatoes must be cooked and gelatinized with as little water as possible, to maintain fluidity. Alpha amylase is added to keep liquid and Glucoamylase is used to liquefy product. Saccrafication takes place in approximately 30 minutes, and 100% gluclose is produced at 140F in a cooker. Another method is to add the enzyme directly to fermentation for a slower conversion. This spoon fed approach helps in avoiding a glucose shock effect of stressing the yeast. Cereal grains would use a similar process but would utilize varying gelatinization temperatures.
Alcohol by Volume
5.8-15%
Recommended Nutrient
Go Ferm @ 2.5lbs / 1000 gal @ yeast rehydration DAP @ 2 lbs / 1000 gal @ start of fermentation Fermaid K @ 2lbs / 1000 gal @ 1/3 depletion of sugar
Recommended Enzyme
*Dependent on fermentation substrate* Visco-Buster Ultra-Ferm Opti-Mash
2lbs active dry yeast / 1000 gal 8 liters liquid yeast / 1000 gal
Pitching Rates
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Gin
Fermentation and Sugar Source
The production of alcohol is not an intrinsic part of the process of manufacturing gin, where a suitable high-quality spirit is selected and redistilled with flavoring materials or processed in some other way. Any material containing carbohydrate which can easily and economically be made available for fermentation can be considered as a suitable source material for spirit production. Materials rich in starch such as grain and potatoes will require a conversion to a more fermentable form while molasses possesses readily fermentable sugars. The transformation of starch granules into a form that is capable of being fermented can be achieved by a number of methods. Traditionally done by enzymes, more modern plants utilize cooking the grain under conditions of elevated temperature and pressure to bring about starch gelatinization. The elevated temperatures and pressures ensure complete gelatinization and consequent higher spirit yield. At the termination of cooking, the gelatinized starch is commonly blown down or discharged from the cooker at high pressures to aid in the breakdown of large solids. The now fermentable sugars are then cooled to a temperature favoring amylolytic enzyme activity and mixed with an enzyme medium in a suitable conversion vessel. Commonly, separation of wort from the grains does not take place after conversion, and the entire contents of the mash tun go forward to the fermenter and still. Enzymatic conversion is allowed to continue during fermentation, leading to an improved attenuation and an enhancement of spirit yield. Commercial enzyme preparations of amylase and amyloglucosidase are available in liquid form and are replacing barley malt as the agent of conversion in grain and potato spirit distilleries. The amylase may be substituted for pre-malt in the cooling stage.
Fermentation Targets
Start Gravity pH Duration 13.5-25 Brix 4.8-5.2 End 1.5 Brix 3.9
Alcohol by Volume
5.8-15%
Recommended Nutrient
Go Ferm @ 2.5lbs / 1000 gal @ yeast rehydration DAP @ 2 lbs / 1000 gal @ start of fermentation Fermaid K @ 2lbs / 1000 gal @ 1/3 depletion of sugar
Recommended Enzyme
Ultra-Ferm Opti-Mash Pro-Max
Pitching Rates
2lbs active dry yeast / 500 gal 4 liters liquid yeast / 500 gal
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Tequila
Fermentation and Sugar Source
The pine or head of the Agave tequilana plant is highly rich in carbohydrates, specifically inulin. Inulin is an oligosaccharide of fructose with a terminal glucose that belongs to the fructans - the most abundant non-structural polysaccharide found in nature after starch. Adjunct sugars used in tequila wort formulation are analyzed for solids content, reducing and fermentable sugar content. The amount of organoleptic compounds produced is higher in slow fermentations compared to more rapid fermentations. The rate of fermentation depends mainly on the yeast strain used, the medium composition (levels of fermentable sugars and yeast nutrients) and the temperature of fermentation. Significant losses in alcohol levels may occur if open fermenters are used. Tequila fermentations are by no means classified as fermentations carried out by pure yeast cultures. Bacteria and wild yeast also play an active role in fermentation. Low levels of bacteria are welcomed as they tend to produce positive organoleptic properties and are attributed to an overall complexity in the final product. The amount and variety are factors of the abundance of organisms introduced by the raw materials and the overall hygiene standards of the distillery. Lactobacillus, Streptococcus, Leuconostoc, and Pediococcus are the most common bacteria, while Brettanomyces is also commonly encountered. A key point is the amounts of these organisms present. Fermentations overrun by bacteria will lower alcohol yields and produce compounds, when at elevated levels, are considered off flavors. Higher alcohols are closely associated with Tequila fermentations. Yeast strain, carbon:nitrogen ratio (higher ratios enhance higher alcohol production) and temperature are the biggest factors.
Fermentation Targets
Gravity 100% Agave Agave + Adjunct Agave Juice pH Duration Start 4-11% w/v 8-16% w/v 20% w/v 4.0-4.5 End 0.5% w/v 0.5% w/v 0.5% w/v 3.9
Alcohol by Volume
4-12%
Recommended Nutrient
Fermaid K @ 2lbs / 1000 gal @ 1/3 depletion of sugar Urea, ammonium sulfate, ammonium phosphate or magnesium sulphate (optional)
Recommended Enzyme
Inulinase if desired but not widely practiced
Pitching Rates
1-2lbs active dry yeast / 1000 gal 8 liters liquid yeast / 1000 gal
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Rum
Fermentation and Sugar Source
For fermentation, concentrated molasses is cut with water to a gravity ranging from 20-25 brix. Cane juice on the other hand, with a sugar content of 12-16% w/ sucrose, can be used as is for fermentation. Although abundant in fermentable sugars, any type of molasses or pure sugar fermentation medium is lacking in essential nutrients required by the yeast for a healthy metabolism. It does however, have a healthy population of bacteria and/or wild yeast that can take over fermentation, producing excess amounts of acetic a butyric acids which are toxic to yeast. An acid addition is usually practiced to keep bacteria in check. Additionally excess salts and minerals can adversely affect yeast performance. A portion of dunder, the naturally fermented residue from a previous wash distillation, is normally pitched with a pure yeast culture for more complexity. Gentle aeration for the first 48 hours is beneficial for fermentation performance. Fermentation temperatures in the Caribbean can reach 90F, however lower temperatures (7580F) will result in a better alcohol yield.
Fermentation Targets
Start Gravity pH Duration 13.5-25 Brix 4.8-5.2 End 0* 3.9
Alcohol by Volume
5-10%
Recommended Nutrient
Go Ferm @ 2.5lbs / 1000 gal @ yeast rehydration DAP @ 2 lbs / 1000 gal @ start of fermentation Fermaid K @ 2lbs / 1000 gal @ 1/3 depletion of sugar
Recommended Enzyme
Ultra-Ferm Opti-Mash Pro-Max
Pitching Rates
2lbs active dry yeast / 500 gal 4 liters liquid yeast / 500 gal *Always adjust pitching rate to Brix concentration*
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Bourbon Whiskey
Fermentation and Sugar Source
In practice, bourbon mash is fermented in grains where the solids are not separated from the liquefied sugar solution. The maize and rye must be cooked before added to the mash containing malted barley in order that the starch in these grains can become exposed to malt enzymes. The transformation of starch granules into a form that is capable of being fermented can be achieved by a number of methods. Traditionally done by enzymes, more modern plants utilize cooking the grain under conditions of elevated temperature and pressure to bring about starch gelatinization. The elevated temperatures and pressures ensure complete gelatinization and consequent higher spirit yield. At the termination of cooking, the gelatinized starch is commonly blown down or discharged from the cooker at high pressures to a waiting mash tun already charged with malted barley and water. Cold water may be needed to achieve a conversion temperature of 60-65C for the enzymes to start their work on the now available starch.
Fermentation Targets
Start Gravity pH Duration 13.5-25 Brix 4.8-5.2 End 1.5 3.9
Alcohol by Volume
8%
Recommended Nutrient
Go Ferm @ 2.5lbs / 1000 gal @ yeast rehydration DAP @ 2 lbs / 1000 gal @ start of fermentation Fermaid K @ 2lbs / 1000 gal @ 1/3 depletion of sugar
Recommended Enzyme
Ultra-Ferm Opti-Mash Pro-Max
Pitching Rates
2lbs active dry yeast / 500 gal 4 liters liquid yeast / 500 gal
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Rye Whiskey
Fermentation and Sugar Source
In nature, starch exists in a tightly packed crystalline structure. Water is required to hydrolyze these starch granules into fermentable material. At specific temperature ranges the starch granules swell or gelatinize into a liquid phase. Enzymes present can now more easily convert the high molecular weight carbohydrates into simpler fermentable sugars. The result is a substantial increase in viscosity. If suitable liquefaction enzymes are not present, the viscosity will overload most mechanical mixing systems. Pre gelatinized flakes are an option for those plants without adequate mashing temperature control. Starch conversion can be achieved by the newer method of pressure cooking, however this requires specialized equipment and the inherent higher energy demand.
Gelatinization Temperatures
Grain Starches Barley Wheat Rye Maize/Corn Rice Sorghum C 52-59 58-64 57-70 62-72 68-77 68-77 F 126-138 136-147 135-158 144-162 154-171 154-171
Fermentation Targets
Start Gravity pH Duration 13.5 Brix 4.8-5.2 End 1.5 Brix 3.9
Alcohol by Volume
8%
Rye grains, like wheat, do not have husks and therefore can be difficult to work with. Rye also is high in beta glucan, a gum like substance that tends to increase viscosities and cause transfer problems. Rye has also been known to cause excess foaming during fermentation. Sufficient freeboard or the use of a non foaming agent may be necessary. Back set, a small amount from the previous ferment, is often added (25% of total fermenter volume) to the new ferment to assist in pH adjustment and with overall consistency of the product. Fermentation temperatures are normally held at 90-95F with slow, constant mixing.
Pitching Rates
2lbs active dried yeast / 500 gallons 4 liters liquid yeast / 500 gallons
Recommended Enzyme
WLN4100 Ultra Ferm WLN4300 Opti-Mash
Superstart Ethanol Technology WLP001 - California Ale Yeast K1 Lallemand 71B Lallemand WLP050 - Tennessee Whiskey Yeast WLP 065 - American Whiskey Yeast
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Brandy
Fermentation and Sugar Source
Grape juices have distinctly varied properties conferred by their composition. Tastes arising from the balances of organic acids, sugars and phenolic compounds; and aroma from a diverse mixture of secondary metabolites. This is typical of fruits. From the total amounts and balance of sugars and organic acids, sweetness and sourness in fruit juices can be estimated. Average grapes contain per 100g by weight: 6.2g gluclose, 6.7g fructose, 1.8g sucrose, 1.9g maltose and 1.6g other mono and oligosaccharides. In apples, sugars constitute between 7 and 14% of the fruit on a fresh weight basis, being almost entirely fructose, glucose and sucrose with traces of other sugars including xylose. The soluble protein content of many fruit juices is very low and in apples is in the range of 10-250ppm but generally <100ppm. Amino acid contents juices decreases with storage. Grape juices are typically between pH 3 and 4; apple juices near pH 3. Carefully monitor the ph of many fruit mashes as high pH conditions are prone to bacterial infections.
Fermentation Targets
Start Gravity pH Duration 20-25 Brix 4.8-5.2 End 1.5 3.9
Alcohol by Volume
5-8%
Recommended Nutrient
RapidaseC80
Recommended Enzyme
Ultra-Ferm Opti-Mash Pro-Max
Pitching Rates
2lbs active dry yeast / 500 gal 4 liters liquid yeast / 500 gal
EC1118 Lallemand DV10 Lallemand WLP720 - Sweet Mead WLP775 - English Cider
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How to Order
Yeastman, White Labs custom-built tracking and ordering technology, makes it fast and easy to order yeast whenever you need it. Yeastman tracks yeast throughout the factory, regularly updates data between the internal and online systems, and provides up-to-the minute availability information for employees and customers alike. Yeastman protects your yeast and gets it to you when you need it.
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info@whitelabs.com
whitelabs.com | yeastman.com
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