Exam Name___________________________________

MULTIPLECHOICE.Choosetheonealternativethatbestcompletesthestatementoranswersthequestion. 1) Ifthereare20centromeresinacellatanaphase,howmanychromosomesarethereineach daughtercellfollowingcytokinesis? A) 20 B) 40 C) 80 D) 10 E) 30 2) Whichofthefollowingareprimarilyresponsibleforcytokinesisinplantcellsbutnotinanimal cells? A) kinetochores B) centriolesandcentromeres C) cyclin- dependentkinases D) actinandmyosin E) Golgi- derivedvesicles 3) Youhavethetechnologynecessarytomeasureeachofthefollowinginasampleofanimalcells: chlorophylls,organelledensity,picogramsofDNA,cellwallcomponents,andenzymaticactivity. WhichwouldyouexpecttoincreasesignificantlyfromMtoG 1 ? A) organelledensityandcellwalls B) chlorophyllandDNA C) cellwallcomponentsandDNA D) chlorophyllandcellwalls E) organelledensityandenzymaticactivity 4) Usingwhichofthefollowingtechniqueswouldenableyourlabgrouptodistinguishbetweena cellinG2 andacellfromthesameorganisminG 1 ? A) spectrophotometry B) radioactive- labelednucleotides C) fluorescencemicroscopy D) electronmicroscopy E) labeledkinetochoreproteins 5) Whydochromosomescoilduringmitosis? A) toallowthechromosomestomovewithoutbecomingentangledandbreaking B) toallowthesisterchromatidstoremainattached C) toprovideforthestructureofthecentromere D) toallowthechromosomestofitwithinthenuclearenvelope E) toincreasetheirpotentialenergy 5) 4) 1)

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UsethedatainTable12.1toanswerthefollowingquestions. Thedatawereobtainedfromastudyofthelengthoftimespentineachphaseofthecellcyclebycellsofthreeeukaryotic organismsdesignatedbeta,delta,andgamma. CellType Beta Delta Gamma G1 18 100 18 S 24 0 48 G2 12 0 14 M 16 0 20

Table12.1:MinutesSpentinCellCyclePhases 6) Thebestconclusionconcerningdeltaisthatthecells A) divideintheG1 phase. B) containnoDNA. C) containnoRNA. D) areactuallyintheG0 phase. E) containonlyonechromosomethatisveryshort. 7) AmutationresultsinacellthatnolongerproducesanormalproteinkinasefortheMphase checkpoint.Whichofthefollowingwouldlikelybetheimmediateresultofthismutation? A) Thecellwouldneverleavemetaphase. B) Thecellwouldundergonormalmitosis,butfailtoenterthenextG 1 phase. C) Thecellwouldprematurelyenteranaphase. D) Thecellwouldneverenterprophase. E) Thecellwouldneverentermetaphase. Usethefollowinginformationtoanswerthequestionsbelow. Theresearchteamestablishedsimilarlymphocyteculturesfromanumberofhumandonors,includinghealthyteenagersof bothgenders,patientsalreadysufferingfromlong - termbacterialinfections,andelderlyvolunteers.Theyfoundthatthe increaseinlymphocyteincorporationafterpathogenintroductionwasslightlylowerinsomeofthefemaleteenagersand significantlylowerineachoftheelderlypersons.Theyrepeatedthestudywithalargernumberofsamplesbutgotthesame results. 8) Whatmightbeamongtheresearchteamsconclusions? A) Theyoungmenhadhigherresponsesbecausetheyaregenerallyhealthier. B) Theyoungwomenshowedtheseresultsbecausetheyhavepoorernutrition. C) Theelderlydonorsamplesrepresentcellsnolongercapableofanycelldivision. D) Thepatientsamplesshouldhavehadthelowestresponsebutdidnot,sotheexperimentis invalid. E) Theelderlypersonssamplesdemonstratedtheirloweredimmuneresponses. 8) 7) 6)

Usethefollowinginformationtoanswerthequestionsbelow. TheletteredcircleinFigure12.1showsadiploidnucleuswithfourchromosomes.Therearetwopairsofhomologous chromosomes,onelongandtheothershort.Onehaploidsetissymbolizedasblackandtheotherhaploidsetisgray.The chromosomesintheunletteredcirclehavenotyetreplicated.Choosethecorrectchromosomalconditionsforthefollowing stages.

Figure12.1 9) Whatisthecorrectchromosomalconditionattheprometaphaseofmitosis? A) A B) B C) C D) D 9) E) E 10)

10) Whatisthecorrectchromosomalconditionforonedaughternucleusattelophaseofmitosis? A) A B) B C) C D) D E) E 11) AgroupofcellsisassayedforDNAcontentimmediatelyfollowingmitosisandisfoundtohave anaverageof8picogramsofDNApernucleus.Howmanypicogramswouldbefoundattheend ofSandtheendofG2 ? A) 8;8 B) 16;8 C) 16;16 D) 8;16 E) 12;16

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ThefollowingquestionsarebasedonFigure12.3.

Figure12.3 12) Atwhichofthenumberedregionswouldyouexpecttofindcellsatmetaphase? A) IandIV B) IIonly C) IIIonly D) IVonly 13) Whatexplainsanchoragedependenceofanimalcellsinvitroorinvivo? A) responseoftheplasmamembranetocellcyclecontrols B) themakeupoftheextracellularmatrixofthesubstrate C) thebindingofcell- surfacephospholipidstothesubstrate D) thebindingofcell- surfacephospholipidstothoseofadjoiningcells E) attachmentofspindlefiberstocentrioles 14) Whatisacleavagefurrow? A) aringofvesiclesformingacellplate B) thespacethatiscreatedbetweentwochromatidsduringanaphase C) agrooveintheplasmamembranebetweendaughternuclei D) themetaphaseplatewherechromosomesattachtothespindle E) theseparationofdividedprokaryotes 15) Throughamicroscope,youcanseeacellplatebeginningtodevelopacrossthemiddleofacelland nucleiformingoneithersideofthecellplate.Thiscellismostlikely A) aplantcellintheprocessofcytokinesis. B) ananimalcellintheprocessofcytokinesis. C) aplantcellinmetaphase. D) ananimalcellintheSphaseofthecellcycle. E) abacterialcelldividing. 16) Whichofthefollowingmostaccuratelydescribesacyclin? A) ItdecreasesinconcentrationwhenMPFactivityincreases. B) ItactivatesaCdkmoleculewhenitisinsufficientconcentration. C) ItactivatesaCdkwhenitsconcentrationisdecreased. D) ItisactivatedtophosphorylatebycomplexingwithaCdk. E) Itispresentinsimilarconcentrationsthroughoutthecellcycle. 12) E) Vonly 13)

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17) AfterwhichcheckpointisthecellfirstcommittedtocontinuethecellcyclethroughM? A) S B) G2 C) previousM D) G1 E) G0 Usethefollowinginformationtoanswerthequestionsbelow.

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NucleotidescanberadiolabeledbeforetheyareincorporatedintonewlyformingDNAandcanthereforebeassayedtotrack theirincorporation.Inasetofexperiments,astudentfacultyresearchteamusedlabeledTnucleotidesandintroduced theseintothecultureofdividinghumancellsatspecifictimes. 18) Theresearchteamusedthesetuptostudytheincorporationoflabelednucleotidesintoacultureof lymphocytesandfoundthatthelymphocytesincorporatedthelabelednucleotideatasignificantly higherlevelafterapathogenwasintroducedintotheculture.Theyconcludedthat A) infectioncauseslymphocytestodividemorerapidly. B) infectioncauseslymphocyteculturestoskipsomepartsofthecellcycle. C) thepresenceofthepathogenmadetheexperimenttoocontaminatedtotrusttheresults. D) theirtissueculturemethodsneededtoberelearned. E) infectioncausescellculturesingeneraltoreproducemorerapidly. 19) Whichofthefollowingisafunctionofthosespindlemicrotubulesthatdonotattachto kinetochores? A) maintaininganappropriatespacingamongthemovingchromosomes B) pullingthepolesofthespindlesclosertooneanother C) maintainingtheregionofoverlapofmicrotubulesinthecellscenter D) providingtheATPneededbythefibersattachedtokinetochores E) producingacleavagefurrowwhentelophaseiscomplete 20) Whichofthefollowingisaproteinsynthesizedatspecifictimesduringthecellcyclethat associateswithakinasetoformacatalyticallyactivecomplex? A) proteinkinase B) Cdk C) MPF D) cyclin E) PDGF 21) Whichofthefollowingdescribe(s)cyclin- dependentkinase(Cdk)? A) Cdkisinactive,orturnedoff,inthepresenceofcyclinanditispresentthroughoutthecell cycle. B) Cdkisanenzymethatattachesphosphategroupstootherproteins. C) Cdkisinactive,orturnedoff,inthepresenceofcyclin. D) Cdkispresentthroughoutthecellcycle. E) Cdkispresentthroughoutthecellcycleandisanenzymethatattachesphosphategroupsto otherproteins. 18)

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22) Whichofthefollowingwasadiscoverythathadtobemadebeforehumanchromosomescouldbe correctlycounted? A) whentoseechromosomesseparatefromoneanother B) howtovisualizespermnuclei C) whentoseechromosomesinpairs D) howtouseahypotonicsolutiontoswellnuclei E) howtovisualizechromosomes 23) Foranewlyevolvingprotist,whatwouldbetheadvantageofusingeukaryote - likecelldivision ratherthanbinaryfission? A) Celldivisionallowsforlowerratesoferrorperchromosomereplication. B) Binaryfissionwouldnotallowtheorganismtohavecomplexcells. C) Celldivisionwouldallowfortheorderlyandefficientsegregationofmultiplelinear chromosomes. D) Celldivisionwouldbefasterthanbinaryfission. E) Binaryfissionwouldnotallowfortheformationofneworganisms. ThefollowingquestionsarebasedonFigure12.3.

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Figure12.3 24) Inthefigureabove,mitosisisrepresentedbywhichnumberedpart(s)ofthecycle? A) I B) II C) III D) IV 25) ThecyclincomponentofMPFisdestroyedtowardtheendofwhichphase? A) G0 B) G1 C) G2 D) M 24) E) V 25) E) S

AnswerKey Testname:CH12

1) D 2) E 3) E 4) B 5) A 6) D 7) B 8) E 9) B 10) E 11) C 12) C 13) A 14) C 15) A 16) B 17) D 18) A 19) C 20) D 21) E 22) D 23) C 24) D 25) D